FastQCFastQC Report
Fri 27 May 2016
SRR940087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660067
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53700.8135537756015677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43220.6547820145530681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.38480942086182157No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.18058772821546903No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.1271083087019954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.105292341535026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.082.598871
GTATCAA37550.055.874081
ATAACGC952.593879E-950.185493
TAACGCA1004.083631E-947.6762124
TGGTATC6600.044.2449722
GTGGTAT7200.043.8753971
ATCAACG47950.043.0030483
TCAACGC48100.042.8689464
TATCAAC48900.042.4113542
CAACGCA49100.042.311825
AACGCAG51300.040.6016
TATAACG1304.1392923E-836.674012
CGCAGAG57500.035.8010378
ACGCAGA58300.035.6159257
GTACATG34050.032.559181
GCAGAGT63800.032.1725739
TACATGG35200.031.829292
ACATGGG36000.029.4665473
GAGTACT45750.029.39034712-13
CAGAGTA63250.028.64271410-11
Produced by FastQC (version 0.11.5)