##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660067 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.122896615040595 34.0 33.0 34.0 31.0 34.0 2 33.17105536256168 34.0 33.0 34.0 31.0 34.0 3 33.224833236625976 34.0 33.0 34.0 31.0 34.0 4 36.45228590430971 37.0 37.0 37.0 35.0 37.0 5 36.466036023615786 37.0 37.0 37.0 35.0 37.0 6 36.55243331358786 37.0 37.0 37.0 35.0 37.0 7 36.54039211170987 37.0 37.0 37.0 35.0 37.0 8 36.54743079111666 37.0 37.0 37.0 35.0 37.0 9 38.419943732984684 39.0 39.0 39.0 37.0 39.0 10-11 38.4009433890802 39.0 39.0 39.0 37.0 39.0 12-13 38.38137719352732 39.0 39.0 39.0 37.0 39.0 14-15 39.97199450964827 41.0 40.0 41.0 38.0 41.0 16-17 39.982872193277345 41.0 40.0 41.0 38.0 41.0 18-19 40.04273354068602 41.0 40.0 41.0 38.0 41.0 20-21 40.04475227514783 41.0 40.0 41.0 38.0 41.0 22-23 39.99557544915895 41.0 40.0 41.0 38.0 41.0 24-25 39.96169782764477 41.0 40.0 41.0 38.0 41.0 26-27 39.8624912319507 41.0 40.0 41.0 37.0 41.0 28-29 39.768487895925716 41.0 40.0 41.0 37.0 41.0 30-31 39.58245678090255 41.0 40.0 41.0 36.5 41.0 32-33 39.361463305997724 41.0 39.5 41.0 35.0 41.0 34-35 39.22857831098964 41.0 39.0 41.0 35.0 41.0 36-37 39.11153943463315 41.0 39.0 41.0 35.0 41.0 38-39 38.97976644795149 41.0 39.0 41.0 35.0 41.0 40-41 38.81954786408046 41.0 39.0 41.0 35.0 41.0 42-43 38.62116270015014 41.0 39.0 41.0 35.0 41.0 44-45 38.355717677144895 41.0 38.0 41.0 35.0 41.0 46-47 37.961790242505685 41.0 38.0 41.0 34.0 41.0 48-49 37.601810876774636 41.0 38.0 41.0 33.0 41.0 50-51 37.57683159436845 41.0 38.0 41.0 33.0 41.0 52-53 37.46076686154587 41.0 38.0 41.0 33.0 41.0 54-55 37.30517659570923 41.0 37.0 41.0 33.0 41.0 56-57 37.11577385326035 40.0 37.0 41.0 33.0 41.0 58-59 36.90174027788088 40.0 36.0 41.0 33.0 41.0 60-61 36.66772918506757 40.0 36.0 41.0 32.0 41.0 62-63 36.40223340963872 39.0 35.0 41.0 32.0 41.0 64-65 36.10477875730797 39.0 35.0 41.0 31.5 41.0 66-67 35.78166231609821 38.5 35.0 41.0 31.0 41.0 68-69 35.43810779814776 37.0 35.0 40.5 31.0 41.0 70-71 35.07944345043761 37.0 35.0 39.5 31.0 41.0 72-73 34.715935655016835 36.0 35.0 39.0 30.5 41.0 74-75 34.35672060563549 36.0 35.0 39.0 30.0 41.0 76-77 34.006474342756114 35.5 35.0 37.5 30.0 39.5 78-79 33.677867549809335 35.0 35.0 37.0 29.5 39.0 80-81 33.37354768531073 35.0 35.0 37.0 29.0 39.0 82-83 33.09816276226505 35.0 35.0 36.0 29.0 38.0 84-85 32.8556495022475 35.0 35.0 36.0 29.0 37.0 86-87 32.651648241769394 35.0 35.0 35.5 29.0 37.0 88-89 32.544949982350275 35.0 34.5 35.0 29.0 36.5 90-91 32.53295347290502 35.0 35.0 35.0 29.0 36.0 92-93 32.44508815014234 35.0 35.0 35.0 29.0 36.0 94-95 32.36364641771214 35.0 35.0 35.0 29.0 36.0 96-97 32.2941405948184 35.0 35.0 35.0 29.0 36.0 98-99 32.23388762049913 35.0 35.0 35.0 29.0 35.5 100-101 32.16919721785818 35.0 35.0 35.0 29.0 35.0 102-103 31.69580739530987 35.0 33.5 35.0 26.0 35.0 104-105 32.06869529911357 35.0 34.0 35.0 29.0 35.0 106-107 32.11031228648001 35.0 35.0 35.0 29.0 35.0 108-109 32.090761998403195 35.0 35.0 35.0 29.0 35.0 110-111 32.04687175695801 35.0 35.0 35.0 29.0 35.0 112-113 32.011762442297524 35.0 35.0 35.0 29.0 35.0 114-115 31.95275934109719 35.0 35.0 35.0 27.0 35.0 116-117 31.896768055364078 35.0 35.0 35.0 27.0 35.0 118-119 31.833650220356418 35.0 34.0 35.0 27.0 35.0 120-121 31.753974217768803 35.0 34.0 35.0 26.0 35.0 122-123 31.647114611092512 35.0 34.0 35.0 25.0 35.0 124-125 30.619116695729375 34.5 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10842339823760483 1101 2 0.09656819617173085 1101 3 0.09028222410712772 1101 4 0.028075805171511092 1101 5 0.05967268199098186 1101 6 0.06629929431159809 1101 7 0.08140167228918216 1101 8 0.12727252482111595 1101 9 0.15395231976241064 1101 10-11 0.1579211238892242 1101 12-13 0.11221115434792495 1101 14-15 0.19869602122252417 1101 16-17 0.1520668932279321 1101 18-19 0.16917422696762685 1101 20-21 0.18863152059473975 1101 22-23 0.2394619241355258 1101 24-25 0.20488740698502284 1101 26-27 0.28424752359043737 1101 28-29 0.19532127697113566 1101 30-31 0.2188633124612025 1101 32-33 0.25214026357672026 1101 34-35 0.30452135377787215 1101 36-37 0.23507808956855314 1101 38-39 0.19948630210829066 1101 40-41 0.13842176675606055 1101 42-43 0.25079661882836035 1101 44-45 0.1802809759491666 1101 46-47 0.14760113420281584 1101 48-49 0.21719119207501336 1101 50-51 0.2508649030222827 1101 52-53 0.136486606283988 1101 54-55 0.21660650580358976 1101 56-57 0.3713457158141793 1101 58-59 0.39587884482214264 1101 60-61 0.33479902437432685 1101 62-63 0.3025176283389257 1101 64-65 0.28451640748059503 1101 66-67 0.22934167199300504 1101 68-69 0.2542163588612496 1101 70-71 0.19308400665303793 1101 72-73 0.2461502613400839 1101 74-75 0.27765863336549046 1101 76-77 0.3319955667712904 1101 78-79 0.3688175802318199 1101 80-81 0.2239536616416018 1101 82-83 0.25348731543395076 1101 84-85 0.2602687333878251 1101 86-87 0.14611752601705064 1101 88-89 0.08413880837519372 1101 90-91 0.19421910564319234 1101 92-93 0.25835076702066573 1101 94-95 0.35008416653523255 1101 96-97 0.23755760361058975 1101 98-99 0.13347563159278053 1101 100-101 0.15208531806698034 1101 102-103 0.1375392536484341 1101 104-105 0.05434161402333615 1101 106-107 0.1048689496655939 1101 108-109 0.051733346184725804 1101 110-111 0.013826264964009738 1101 112-113 0.002317889280732288 1101 114-115 0.0040997760825263185 1101 116-117 0.01205000782191945 1101 118-119 0.019530652325272513 1101 120-121 -0.056569821037872 1101 122-123 -0.03893811826791449 1101 124-125 0.048609204572304776 1102 1 0.139228604691219 1102 2 0.13289225565575435 1102 3 0.15254092682582865 1102 4 -0.08149127451932259 1102 5 0.0999703766724096 1102 6 0.12837647588307988 1102 7 0.14369698431487876 1102 8 0.17284653587407917 1102 9 0.162491440620407 1102 10-11 0.1652170536969777 1102 12-13 0.20267198925367325 1102 14-15 0.25998152653011175 1102 16-17 0.25624248976896524 1102 18-19 0.2902221378727745 1102 20-21 0.25570053371460233 1102 22-23 0.22959495320483825 1102 24-25 0.20872909613844115 1102 26-27 0.16362138240968704 1102 28-29 0.2257504753340882 1102 30-31 0.2585508520259481 1102 32-33 0.25702302989799364 1102 34-35 0.06896563212453799 1102 36-37 -0.0012868503082898997 1102 38-39 0.07495059201739451 1102 40-41 0.02023700873595402 1102 42-43 0.04106418710090054 1102 44-45 0.03927870464674754 1102 46-47 0.08176463441864712 1102 48-49 0.13395001758743064 1102 50-51 0.1502167657495903 1102 52-53 0.27012888149140224 1102 54-55 0.1995305521692714 1102 56-57 0.05491393344431117 1102 58-59 -0.062383494421048624 1102 60-61 -0.01898416615728138 1102 62-63 -0.014406854287670967 1102 64-65 -0.012321006010843405 1102 66-67 0.06459902779429427 1102 68-69 0.03826873457646229 1102 70-71 -0.03191069262437907 1102 72-73 0.03449584099507064 1102 74-75 -0.02969632894979668 1102 76-77 0.038943403084978456 1102 78-79 -0.03329675978626767 1102 80-81 -0.12136820280939276 1102 82-83 -0.0908971982190323 1102 84-85 -0.07537149474917015 1102 86-87 0.021826640666724018 1102 88-89 -0.12050492967911453 1102 90-91 0.022408577111576733 1102 92-93 0.026372079738898435 1102 94-95 0.0011326418338626354 1102 96-97 -0.02156688481247926 1102 98-99 -0.01222559680200419 1102 100-101 0.01780848153821779 1102 102-103 -0.02837107490484314 1102 104-105 0.06645090500784079 1102 106-107 0.019629517170869804 1102 108-109 0.056503233809415576 1102 110-111 0.1432050812935941 1102 112-113 0.1369120064701761 1102 114-115 0.11442344811804972 1102 116-117 0.10089011819898275 1102 118-119 0.047723632185579845 1102 120-121 0.05464849093748825 1102 122-123 0.09608005672762232 1102 124-125 -0.01778101098024365 1103 1 0.12552106605302527 1103 2 0.0747059823556242 1103 3 0.1251505268931865 1103 4 -0.10537604003375378 1103 5 0.03640153922207645 1103 6 0.14417142747920053 1103 7 0.07135172214713492 1103 8 0.14663339039823597 1103 9 0.2212075510065219 1103 10-11 0.19112390978887106 1103 12-13 0.16949402641195377 1103 14-15 0.16071255654792793 1103 16-17 0.19759895980558895 1103 18-19 0.23836923226071605 1103 20-21 0.15403132915248818 1103 22-23 0.18542250792684456 1103 24-25 0.15262950999201053 1103 26-27 0.18629623227708692 1103 28-29 0.21737324294173987 1103 30-31 0.04701085513239178 1103 32-33 0.06270999634115526 1103 34-35 0.13600453958594727 1103 36-37 0.0782598414034652 1103 38-39 0.11351474803046813 1103 40-41 0.005307941583097886 1103 42-43 0.09924610557567348 1103 44-45 0.07975535479895512 1103 46-47 -0.05193285908902823 1103 48-49 -0.14529227183135873 1103 50-51 -0.11343234564829885 1103 52-53 -0.1598448008473099 1103 54-55 -0.1316394006969972 1103 56-57 -0.11846835780350773 1103 58-59 -0.04593023839071009 1103 60-61 -0.08008599503877178 1103 62-63 -0.13915010104223313 1103 64-65 -0.06358891341564998 1103 66-67 0.1348612318294684 1103 68-69 0.06107785822592149 1103 70-71 0.04761785398168428 1103 72-73 0.003594000210391357 1103 74-75 0.07164771531844849 1103 76-77 -0.04975350252127697 1103 78-79 0.12181558745008658 1103 80-81 0.05445351644663532 1103 82-83 0.1399840297255892 1103 84-85 0.03658090410637982 1103 86-87 -0.05743045515831113 1103 88-89 -0.0516776406014543 1103 90-91 0.014135860527964894 1103 92-93 0.036949950548645916 1103 94-95 -0.06524796119332166 1103 96-97 -0.09050159291579263 1103 98-99 -0.03254521195157167 1103 100-101 0.03776156555295529 1103 102-103 -0.12648819357819008 1103 104-105 -0.2344580922065269 1103 106-107 -0.13744685630476283 1103 108-109 -0.08353996605820058 1103 110-111 -0.017089508126211683 1103 112-113 -0.030811915021033087 1103 114-115 -0.0078085160098062545 1103 116-117 -0.020015801605460126 1103 118-119 -0.07832461056980833 1103 120-121 -0.0979242673072882 1103 122-123 -0.08360439262648356 1103 124-125 -0.02368252515302416 1104 1 0.04853739863132489 1104 2 0.10947302881008625 1104 3 0.16728767059375826 1104 4 0.07917017315833874 1104 5 0.03206293200440058 1104 6 0.05459203252827649 1104 7 0.06833726296154907 1104 8 0.07110302156694814 1104 9 0.06241197221332584 1104 10-11 0.14661876931279494 1104 12-13 0.10375284413083818 1104 14-15 0.09454986090611328 1104 16-17 0.16087194718572562 1104 18-19 0.14737485811580342 1104 20-21 0.11124204609420474 1104 22-23 0.14608197054698735 1104 24-25 0.198751161302539 1104 26-27 0.14566560855084987 1104 28-29 0.18792407716919257 1104 30-31 0.21393582291570823 1104 32-33 0.11260610819972783 1104 34-35 0.16855828608752432 1104 36-37 0.022790386220123082 1104 38-39 0.11926620558676859 1104 40-41 0.0509647928073349 1104 42-43 0.07036534418345752 1104 44-45 0.11635037838667017 1104 46-47 0.08954802946681184 1104 48-49 -0.005675550949838737 1104 50-51 0.07670708018522276 1104 52-53 0.014881712201145092 1104 54-55 -0.053858831753309744 1104 56-57 0.031411574754358185 1104 58-59 -0.06317183792747016 1104 60-61 0.042555942844060723 1104 62-63 -0.011357042813784801 1104 64-65 -0.017490679514047258 1104 66-67 -0.233724526979735 1104 68-69 -0.20007237651586252 1104 70-71 -0.07164700968641569 1104 72-73 -0.15338353610788857 1104 74-75 -0.11389409256229044 1104 76-77 -0.12005611221410106 1104 78-79 -0.118347681563435 1104 80-81 -0.012738835569535922 1104 82-83 0.006161343507514516 1104 84-85 -0.047223615879318004 1104 86-87 -0.019866092764679877 1104 88-89 0.0037158854074874625 1104 90-91 -0.07031654110472374 1104 92-93 -0.14874726377315284 1104 94-95 -0.1334451329380144 1104 96-97 -0.06349351461933139 1104 98-99 -0.0917869988276081 1104 100-101 -0.1203023966158554 1104 102-103 -0.04740192668217347 1104 104-105 -0.06624019960302263 1104 106-107 -0.03951738102502844 1104 108-109 -0.12620435626887172 1104 110-111 -0.14137847043695473 1104 112-113 -0.08199527475217394 1104 114-115 -0.05730369344280106 1104 116-117 -0.13623924058678938 1104 118-119 -0.10855648549529562 1104 120-121 -0.10294623709457085 1104 122-123 -0.11452601668765539 1104 124-125 -0.19496596947068312 1105 1 0.14997388641046427 1105 2 0.13806809648389162 1105 3 0.11013052004016544 1105 4 0.07089098986183728 1105 5 0.019174123745010263 1105 6 0.0812822283480017 1105 7 0.13363066270427026 1105 8 0.14108301079191676 1105 9 0.16313104072274598 1105 10-11 0.18162525632229176 1105 12-13 0.22086123216395492 1105 14-15 0.29403777197911296 1105 16-17 0.23896344700430916 1105 18-19 0.22709360777221121 1105 20-21 0.22617068898982495 1105 22-23 0.2036665490562939 1105 24-25 0.19717201428930764 1105 26-27 0.25743595742002867 1105 28-29 0.333769092617068 1105 30-31 0.3555093075393003 1105 32-33 0.3075957179896136 1105 34-35 0.20620905408348023 1105 36-37 0.14186549259608938 1105 38-39 0.1968780515257862 1105 40-41 0.24970092545018474 1105 42-43 0.16587968707138145 1105 44-45 0.10993483595172648 1105 46-47 0.18713629127812226 1105 48-49 0.03560904185287228 1105 50-51 0.2232640174371454 1105 52-53 0.10977621583497665 1105 54-55 0.07432145213581975 1105 56-57 0.10655918170755285 1105 58-59 0.0290990002161422 1105 60-61 0.09355593184912436 1105 62-63 0.1850302976222764 1105 64-65 0.14270821879382822 1105 66-67 0.11099955521837757 1105 68-69 0.046440855884007703 1105 70-71 0.19824694420084654 1105 72-73 0.04028668192160012 1105 74-75 -0.027752928638854257 1105 76-77 0.03365440223873861 1105 78-79 -0.041754241139472015 1105 80-81 0.030955021562327545 1105 82-83 -0.018632226656642104 1105 84-85 0.02527696135457802 1105 86-87 -0.12728382319095033 1105 88-89 -0.11578664892418544 1105 90-91 -0.06196451638812306 1105 92-93 0.03267460074729911 1105 94-95 0.004222402328217356 1105 96-97 -0.05636605038034759 1105 98-99 -0.017711397679732954 1105 100-101 0.07869349127982161 1105 102-103 0.04539941689843374 1105 104-105 0.02807981886846278 1105 106-107 -0.012094647904994815 1105 108-109 -0.03002482003508078 1105 110-111 0.07203726990674397 1105 112-113 0.09853600033000731 1105 114-115 0.048323237542010844 1105 116-117 0.14651328549869191 1105 118-119 0.13507332616153178 1105 120-121 0.16837430913361828 1105 122-123 0.1927974322024042 1105 124-125 0.1172680906276149 1106 1 0.1417771650989863 1106 2 0.18256458360333028 1106 3 0.15579796734695606 1106 4 -0.012247183440265985 1106 5 0.048448128428852044 1106 6 0.052008223664159914 1106 7 0.10669857839513952 1106 8 0.1574764534148656 1106 9 0.158447199973331 1106 10-11 0.151765771544774 1106 12-13 0.21676287150822304 1106 14-15 0.2173398797074455 1106 16-17 0.23486508634857728 1106 18-19 0.2821287365778318 1106 20-21 0.17406296065259141 1106 22-23 0.18434570596495803 1106 24-25 0.2557200236569912 1106 26-27 0.26504719863781645 1106 28-29 0.27170820268732854 1106 30-31 0.1564460756891819 1106 32-33 0.1899142191605847 1106 34-35 0.235483058767322 1106 36-37 0.22617462608555172 1106 38-39 0.13657360187708179 1106 40-41 0.23447844301458076 1106 42-43 0.22384221634538903 1106 44-45 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2216 124-125 -1.2644677433258913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 10.0 5 42.0 6 82.0 7 186.0 8 353.0 9 597.0 10 1149.0 11 1804.0 12 2445.0 13 3895.0 14 7411.0 15 7750.0 16 3323.0 17 2377.0 18 2185.0 19 1773.0 20 1614.0 21 1421.0 22 1356.0 23 1437.0 24 1796.0 25 2204.0 26 2671.0 27 3237.0 28 3885.0 29 4690.0 30 5786.0 31 7130.0 32 9111.0 33 11971.0 34 17724.0 35 34064.0 36 79665.0 37 253112.0 38 179658.0 39 2151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.17393063382363 18.41072086091762 13.991578111575626 22.423770393683128 2 15.731886165854606 19.415254648146803 41.53561559159334 23.317243594405255 3 19.759084697590847 24.969775749697757 29.039065790390655 26.23207376232074 4 12.377174152147035 16.650459293196697 37.40277472256567 33.5695918320906 5 13.091054034679312 37.59678820036464 33.87515023104891 15.43700753390713 6 31.17937264575486 36.8624408692411 16.96344423601999 14.994742248984059 7 27.858081073587986 33.559774992538635 20.171134142443115 18.411009791430264 8 26.432619718907326 35.36368277765742 18.822028672846848 19.38166883058841 9 26.95302143570274 16.26653051887157 19.88631457109657 36.894133474329124 10-11 25.414162501685432 26.913252745554615 26.095229726679257 21.577355026080685 12-13 26.541851054514165 24.239584769424923 27.297986416530446 21.92057775953047 14-15 23.10947222024431 25.019657095416072 26.70175906385261 25.169111620487012 16-17 21.562583798311383 28.583234732231727 27.06854001184728 22.785641457609607 18-19 21.373739332522305 25.848588097874913 31.238646985836287 21.539025583766495 20-21 22.40454378116161 24.836190871532736 31.732990741848933 21.02627460545672 22-23 21.884217814252192 24.914061754337062 31.49475735039019 21.706963081020564 24-25 21.47418368135356 25.213804053224898 31.924940952963865 21.387071312457675 26-27 21.34760562185354 25.498623624571444 31.57452198034442 21.579248773230596 28-29 21.213225324095887 25.481201150792266 31.77541067800693 21.530162847104915 30-31 21.512074919723997 25.25889436897646 31.69945755465548 21.529573156644066 32-33 21.418204515602206 25.23584727004986 31.575203729318385 21.77074448502955 34-35 21.671577799156143 25.44734230719702 31.402210388370843 21.478869505275995 36-37 21.66757945625011 25.23548835000216 31.28504865812356 21.811883535624172 38-39 21.661425929867807 25.367564901935978 31.279812924255385 21.691196243940833 40-41 21.94943846609511 25.27978220392778 31.08154172227971 21.6892376076974 42-43 21.76631179939975 25.465072061739935 30.798534676445588 21.970081462414722 44-45 21.940438928464296 25.59668637188628 30.52781405383131 21.93506064581812 46-47 22.155856905435357 25.945472202064334 29.768720448075726 22.12995044442458 48-49 22.296096537409763 26.04010271871283 29.392399118255298 22.271401625622104 50-51 22.36265696644287 26.44573508056544 28.927200598133336 22.264407354858356 52-53 22.502791383298966 26.414969995470155 28.875477792405924 22.206760828824955 54-55 22.36515384044347 26.459207928892063 28.891461018351166 22.284177212313296 56-57 22.309225965478948 26.570347286084168 28.708255752119882 22.41217099631701 58-59 22.42424150604947 26.201561074935835 28.987907123313406 22.386290295701286 60-61 22.408576770060996 26.224506182752528 29.07901075959531 22.287906287591166 62-63 22.240166528549373 26.25710723305361 29.28763292211245 22.21509331628456 64-65 22.166983178057162 26.23017132520428 29.214443221492576 22.38840227524598 66-67 22.317044533492915 26.444895578465754 28.876853037200885 22.36120685084045 68-69 22.35699070693359 26.207916832055172 29.069466201659843 22.365626259351394 70-71 22.372377035706627 26.19733524023213 28.969964071420833 22.460323652640408 72-73 22.436661732824092 26.336417363691865 28.78791092419406 22.43900997928998 74-75 22.18852191407987 26.664661818165293 28.560758650832906 22.58605761692193 76-77 22.296627810788944 26.51777300369974 28.64482172454647 22.540777460964843 78-79 22.393300038556895 26.50298039506805 28.620655436939025 22.48306412943603 80-81 22.25698300324058 26.81462639398728 28.391284521116795 22.53710608165535 82-83 22.37197133018315 26.682215593265536 28.414691235889688 22.53112184066163 84-85 22.46802218562661 26.493068127932467 28.361893565350183 22.677016121090737 86-87 22.368200779769918 26.472559961761426 28.438649742109316 22.720589516359336 88-89 22.30697792799822 26.591921729157797 28.343789342597038 22.757311000246943 90-91 22.369202473692024 26.53704326537043 28.525405035254046 22.56834922568349 92-93 22.30887226588391 26.455979547391344 28.566310008521924 22.668838178202822 94-95 22.070090279261223 26.519362545696396 28.471600438139994 22.938946736902384 96-97 22.28705570798116 26.367550566836396 28.582931732687744 22.7624619924947 98-99 22.242211775471276 26.30157241613351 28.347273837352876 23.108941971042334 100-101 22.07860874623996 26.401279265043748 28.503794693413465 23.01631729530282 102-103 22.375152825395 26.06205127661283 28.515211334606942 23.047584563385232 104-105 22.197367842961395 26.191886581210692 28.360378567630256 23.250367008197653 106-107 22.229595222295952 26.22586226225862 28.34731678347317 23.197225731972257 108-109 22.310302773211728 26.039405210092948 28.150182179027823 23.5001098376675 110-111 22.175400376022434 26.266575968803167 28.337577851945333 23.22044580322907 112-113 22.104624465299278 26.345112719874013 28.30575147466426 23.244511340162454 114-115 21.952278183089426 26.430696253112433 28.163507710348163 23.45351785344998 116-117 21.75904287996534 26.376628915248308 28.3832540354072 23.481074169379156 118-119 21.890505054789895 26.467388916579683 28.095102466870784 23.547003561759638 120-121 21.690785466907855 26.565752515657525 28.200892032008923 23.5425699854257 122-123 21.827421934002103 26.602849864369727 28.094820749121492 23.47490745250668 124-125 21.764421150389317 26.561989869156527 27.99306430366169 23.680524676792462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 273.0 1 200.0 2 362.0 3 528.5 4 290.0 5 191.0 6 503.5 7 1276.5 8 2375.5 9 2734.0 10 1818.0 11 926.5 12 752.0 13 797.0 14 878.0 15 958.0 16 1027.5 17 1068.0 18 1143.5 19 1236.0 20 1328.5 21 1547.5 22 1822.5 23 2179.0 24 2592.0 25 3088.5 26 3852.0 27 4756.5 28 5634.0 29 6527.5 30 7468.5 31 8625.0 32 10163.5 33 11425.0 34 12573.5 35 13531.0 36 14710.0 37 16105.0 38 16759.0 39 17673.0 40 18751.0 41 19677.5 42 20514.5 43 21452.5 44 22463.5 45 23053.5 46 23437.5 47 23678.0 48 24002.5 49 24276.5 50 24047.0 51 23585.0 52 22992.0 53 22452.0 54 22215.5 55 21064.0 56 19045.0 57 17405.5 58 16166.0 59 14207.0 60 11857.0 61 9930.0 62 8104.5 63 6129.0 64 4426.0 65 3362.0 66 2595.5 67 2027.0 68 1432.5 69 976.0 70 752.0 71 586.0 72 467.5 73 418.0 74 330.0 75 247.5 76 170.5 77 99.0 78 53.5 79 21.0 80 14.0 81 9.5 82 4.5 83 2.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018179972639141177 2 0.0028784956678640197 3 1.5149977199284314E-4 4 0.13665279433754451 5 0.03787494299821079 6 0.01348347970736304 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 7.574988599642157E-5 32-33 0.0 34-35 3.029995439856863E-4 36-37 3.787494299821079E-4 38-39 0.0020452469219033828 40-41 0.0 42-43 0.0012119981759427451 44-45 7.574988599642158E-4 46-47 0.0 48-49 0.0018179972639141178 50-51 0.0020452469219033828 52-53 0.0 54-55 0.0 56-57 9.089986319570589E-4 58-59 0.0013634979479355885 60-61 7.574988599642158E-4 62-63 0.0 64-65 9.847485179534806E-4 66-67 3.029995439856863E-4 68-69 7.574988599642158E-4 70-71 0.0011362482899463235 72-73 0.0 74-75 7.574988599642157E-5 76-77 0.0031814952118497065 78-79 5.30249201974951E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 7.574988599642157E-5 88-89 0.0 90-91 1.5149977199284314E-4 92-93 6.817489739677943E-4 94-95 6.059990879713726E-4 96-97 0.0 98-99 0.0 100-101 7.574988599642157E-5 102-103 0.0 104-105 0.0 106-107 1.5149977199284314E-4 108-109 3.029995439856863E-4 110-111 0.0 112-113 2.2724965798926472E-4 114-115 0.0018937471499105392 116-117 5.30249201974951E-4 118-119 0.0 120-121 1.5149977199284314E-4 122-123 7.574988599642157E-5 124-125 5.30249201974951E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 660067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.44143843437811 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42691993422903 48.34648254236685 2 13.50684408302039 16.867735465053237 3 4.277186364547693 8.012210071627988 4 1.815127714933741 4.533567418502745 5 0.8939233918153551 2.7908931217544475 6 0.5447530019466914 2.0409096619798195 7 0.3310646245812943 1.4470505961515374 8 0.22588827917914117 1.1283831261929567 9 0.1653747097142712 0.9293611279704135 >10 0.7622409526659198 8.22925934014155 >50 0.030794203426473207 1.3137331140801007 >100 0.018185432872070983 2.012906584646346 >500 7.274173148313347E-4 0.31507022207391433 >1k 7.274173148313347E-4 1.21940200316353 >5k 2.4247243827711155E-4 0.8130356042945314 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5370 0.8135537756015677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4322 0.6547820145530681 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2540 0.38480942086182157 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1192 0.18058772821546903 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.1271083087019954 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 0.105292341535026 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.029995439856863E-4 0.0 14-15 0.0 0.0 0.0 6.059990879713726E-4 0.0 16-17 0.0 0.0 0.0 6.059990879713726E-4 0.0 18-19 0.0 0.0 0.0 6.817489739677941E-4 0.0 20-21 0.0 0.0 0.0 7.574988599642157E-4 0.0 22-23 0.0 0.0 0.0 7.574988599642157E-4 0.0 24-25 0.0 0.0 0.0 9.847485179534804E-4 0.0 26-27 0.0 0.0 0.0 0.0013634979479355883 0.0 28-29 0.0 0.0 0.0 0.002120996807899804 0.0 30-31 0.0 0.0 0.0 0.0039389940718139215 0.0 32-33 0.0 0.0 0.0 0.00924148609156343 0.0 34-35 0.0 0.0 0.0 0.015528726629266423 0.0 36-37 0.0 0.0 0.0 0.02552771158079407 0.0 38-39 0.0 0.0 0.0 0.044692432737888727 0.0 40-41 0.0 0.0 0.0 0.06870514659875437 0.0 42-43 0.0 0.0 0.0 0.09112711285369515 0.0 44-45 0.0 0.0 0.0 0.1176395729524427 0.0 46-47 0.0 0.0 0.0 0.15119677244885746 0.0 48-49 0.0 0.0 0.0 0.19013221385101814 0.0 50-51 0.0 0.0 0.0 0.23073415274510012 0.0 52-53 0.0 0.0 0.0 0.27262383970112125 0.0 54-55 0.0 0.0 0.0 0.3208765170808418 0.0 56-57 0.0 0.0 0.0 0.3764011835162188 0.0 58-59 0.0 0.0 0.0 0.43647084311138107 0.0 60-61 0.0 0.0 0.0 0.5034337423322177 0.0 62-63 0.0 0.0 0.0 0.5706996410970402 0.0 64-65 0.0 0.0 0.0 0.6350870441939984 0.0 66-67 0.0 0.0 0.0 0.7143214249462555 0.0 68-69 0.0 0.0 0.0 0.8071150352918719 0.0 70-71 0.0 0.0 0.0 0.9130891258008657 0.0 72-73 0.0 0.0 0.0 1.0276987033134515 0.0 74-75 0.0 0.0 0.0 1.1366270393763056 0.0 76-77 0.0 0.0 0.0 1.250024618712949 0.0 78-79 0.0 0.0 0.0 1.3937221524481607 0.0 80-81 0.0 0.0 0.0 1.5584781544903774 0.0 82-83 0.0 0.0 0.0 1.731945393422183 0.0 84-85 0.0 0.0 0.0 1.8978376437543463 0.0 86-87 0.0 0.0 0.0 2.0725926307480904 0.0 88-89 0.0 0.0 0.0 2.274844826358536 0.0 90-91 0.0 0.0 0.0 2.5073969763675503 0.0 92-93 0.0 0.0 0.0 2.7535083559699243 0.0 94-95 0.0 0.0 0.0 3.0076492234879186 0.0 96-97 0.0 0.0 0.0 3.262168840435895 0.0 98-99 0.0 0.0 0.0 3.5328231831011094 0.0 100-101 0.0 0.0 0.0 3.8496849562241406 0.0 102-103 0.0 0.0 0.0 4.19813443180768 0.0 104-105 0.0 0.0 0.0 4.548932153857108 0.0 106-107 0.0 0.0 0.0 4.900108625336519 0.0 108-109 0.0 0.0 0.0 5.267116822989181 0.0 110-111 0.0 0.0 0.0 5.676544956799839 0.0 112-113 0.0 0.0 0.0 6.135513516052159 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1970 0.0 82.59887 1 GTATCAA 3755 0.0 55.87408 1 ATAACGC 95 2.593879E-9 50.18549 3 TAACGCA 100 4.083631E-9 47.676212 4 TGGTATC 660 0.0 44.244972 2 GTGGTAT 720 0.0 43.875397 1 ATCAACG 4795 0.0 43.003048 3 TCAACGC 4810 0.0 42.868946 4 TATCAAC 4890 0.0 42.411354 2 CAACGCA 4910 0.0 42.31182 5 AACGCAG 5130 0.0 40.601 6 TATAACG 130 4.1392923E-8 36.67401 2 CGCAGAG 5750 0.0 35.801037 8 ACGCAGA 5830 0.0 35.615925 7 GTACATG 3405 0.0 32.55918 1 GCAGAGT 6380 0.0 32.172573 9 TACATGG 3520 0.0 31.82929 2 ACATGGG 3600 0.0 29.466547 3 GAGTACT 4575 0.0 29.390347 12-13 CAGAGTA 6325 0.0 28.642714 10-11 >>END_MODULE