FastQCFastQC Report
Fri 27 May 2016
SRR940086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832842
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16000.037.66681
ATCAACG17050.034.6179963
GTACATG34150.034.4217451
GGTATCA11250.033.94571
TCAACGC17500.033.729854
CAACGCA17500.033.7095535
TACATGG34450.033.080752
AACGCAG18500.032.2095076
TATCAAC19450.030.3701382
ACATGGG35950.029.8513983
GAGTACA24800.027.9102481
ACGCAGA21350.027.9098787
CGCAGAG22400.026.6016038
AGTACAT25100.026.1487052
GTGTAGC6750.025.636081
CATGGGG21100.024.8666634
CATGGGA16850.024.0616994
CGGTTAC1252.0203515E-823.78851128-29
CTGTTCG1852.6688456E-522.5358079
ATTCGCG953.313744E-521.91507580-81
Produced by FastQC (version 0.11.5)