##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832842 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52046126396123 34.0 31.0 34.0 30.0 34.0 2 31.58166374894638 34.0 31.0 34.0 30.0 34.0 3 31.808159290717807 34.0 31.0 34.0 30.0 34.0 4 34.95565785587182 37.0 35.0 37.0 33.0 37.0 5 35.071198378564 37.0 37.0 37.0 33.0 37.0 6 35.06350904493289 37.0 37.0 37.0 33.0 37.0 7 35.029244442523314 37.0 37.0 37.0 33.0 37.0 8 34.97776889253904 37.0 37.0 37.0 33.0 37.0 9 36.60985517060859 39.0 39.0 39.0 33.0 39.0 10-11 36.61815686528777 39.0 39.0 39.0 33.0 39.0 12-13 36.565857629658446 39.0 39.0 39.0 33.0 39.0 14-15 38.06140420391863 41.0 40.0 41.0 33.0 41.0 16-17 38.10415240825991 41.0 40.0 41.0 33.0 41.0 18-19 38.12999764661244 41.0 40.0 41.0 33.0 41.0 20-21 38.13035545757779 41.0 40.0 41.0 33.0 41.0 22-23 38.15387492465558 41.0 39.5 41.0 33.0 41.0 24-25 38.205578008793985 41.0 39.0 41.0 33.0 41.0 26-27 38.22781752121051 41.0 39.0 41.0 33.0 41.0 28-29 38.18158186066505 41.0 39.0 41.0 33.0 41.0 30-31 38.12478177133238 41.0 39.0 41.0 33.0 41.0 32-33 38.05985048784763 41.0 39.0 41.0 33.0 41.0 34-35 37.971845800283845 41.0 39.0 41.0 32.5 41.0 36-37 37.84800838574424 41.0 39.0 41.0 32.0 41.0 38-39 37.75096416847373 41.0 38.0 41.0 32.0 41.0 40-41 37.65598036602381 41.0 38.0 41.0 31.5 41.0 42-43 37.50173622367748 40.5 38.0 41.0 31.0 41.0 44-45 37.35526606487185 40.0 38.0 41.0 31.0 41.0 46-47 37.19080389797825 40.0 38.0 41.0 30.0 41.0 48-49 37.034804920981415 40.0 37.0 41.0 30.0 41.0 50-51 36.867601537866726 40.0 37.0 41.0 30.0 41.0 52-53 36.99484055799299 40.0 37.0 41.0 30.0 41.0 54-55 36.80053599602326 40.0 36.5 41.0 30.0 41.0 56-57 36.68210236755591 40.0 36.0 41.0 30.0 41.0 58-59 36.48589048102762 40.0 35.5 41.0 30.0 41.0 60-61 36.23754805833519 39.0 35.0 41.0 30.0 41.0 62-63 35.95061308147284 39.0 35.0 41.0 30.0 41.0 64-65 35.63788209528337 38.5 35.0 41.0 30.0 41.0 66-67 35.3130773904294 37.5 35.0 40.5 29.5 41.0 68-69 34.95996959807502 37.0 35.0 40.0 29.0 41.0 70-71 34.59996433897426 36.5 35.0 39.0 29.0 41.0 72-73 34.22466146039705 36.0 35.0 39.0 29.0 41.0 74-75 33.85629927405198 35.5 34.0 38.5 28.0 40.5 76-77 33.49651914768948 35.0 34.0 37.0 28.0 39.5 78-79 33.150804114105675 35.0 34.0 37.0 27.5 39.0 80-81 32.8344962189707 35.0 34.0 36.5 27.0 39.0 82-83 32.53350395393124 35.0 34.0 36.0 26.5 37.0 84-85 32.29667091717276 35.0 34.0 36.0 26.0 37.0 86-87 32.08500231736632 35.0 34.0 35.0 26.0 37.0 88-89 31.911210049445152 35.0 33.5 35.0 26.0 36.0 90-91 31.756325329414224 35.0 33.0 35.0 25.0 36.0 92-93 31.610305436085117 35.0 33.0 35.0 25.0 36.0 94-95 31.493048501396423 35.0 33.0 35.0 25.0 36.0 96-97 31.390054776296104 35.0 33.0 35.0 24.5 35.0 98-99 31.31992802956623 35.0 33.0 35.0 24.0 35.0 100-101 31.221644081350362 35.0 33.0 35.0 24.0 35.0 102-103 30.52381123910658 34.0 31.0 35.0 23.5 35.0 104-105 31.441699025745578 35.0 33.5 35.0 25.0 35.0 106-107 31.553530561619134 35.0 34.0 35.0 25.0 35.0 108-109 31.54040742421732 35.0 34.0 35.0 25.0 35.0 110-111 31.491219222853793 35.0 34.0 35.0 25.0 35.0 112-113 31.442244747503125 35.0 34.0 35.0 25.0 35.0 114-115 31.379800130156738 35.0 34.0 35.0 24.0 35.0 116-117 31.30619553288619 35.0 34.0 35.0 24.0 35.0 118-119 31.22362645015501 35.0 34.0 35.0 23.5 35.0 120-121 31.134753650752486 35.0 33.5 35.0 23.0 35.0 122-123 30.999944167080912 35.0 33.0 35.0 19.5 35.0 124-125 29.749093465507265 34.5 31.0 35.0 9.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.13816003300735247 1101 2 0.05091935368353262 1101 3 0.07782447654723512 1101 4 -0.04308311128301057 1101 5 -0.10138425413340002 1101 6 0.19599849851554296 1101 7 0.1473704638878388 1101 8 0.1424845088397788 1101 9 0.18269353499954377 1101 10-11 -0.04089726262041893 1101 12-13 -0.02319433814615479 1101 14-15 0.1327991975049727 1101 16-17 0.11202871343974152 1101 18-19 0.17363078521922404 1101 20-21 -0.06855038710148165 1101 22-23 -0.11344712968433868 1101 24-25 0.05369308747893342 1101 26-27 0.022520472403037672 1101 28-29 -0.06988121735436437 1101 30-31 -0.13394443046004056 1101 32-33 0.0951508630033544 1101 34-35 0.1093221073994286 1101 36-37 0.10763796171880102 1101 38-39 0.020504406590404756 1101 40-41 0.011523344938396463 1101 42-43 0.02560994408070627 1101 44-45 -0.013328927542268332 1101 46-47 -0.005331626415923552 1101 48-49 -0.10024778435121107 1101 50-51 -0.1322532308713562 1101 52-53 -0.22190665636482976 1101 54-55 0.06770301613689611 1101 56-57 -0.002611892098975943 1101 58-59 0.11734255369515978 1101 60-61 0.023174755143813286 1101 62-63 0.061125237648440134 1101 64-65 0.10276895703486133 1101 66-67 0.018736313689913686 1101 68-69 0.1618443255417361 1101 70-71 0.06682089266730884 1101 72-73 0.018606628370847034 1101 74-75 -0.02654341598491783 1101 76-77 -0.021187213249696413 1101 78-79 0.07213324377540431 1101 80-81 0.04276115658470303 1101 82-83 0.10169213965983204 1101 84-85 0.05125668832918251 1101 86-87 0.10650822424500461 1101 88-89 0.06812881870782306 1101 90-91 0.05513828204024662 1101 92-93 -0.10270393465433969 1101 94-95 -0.03197781962841617 1101 96-97 0.06540065809946327 1101 98-99 0.10300579528955822 1101 100-101 0.11282079109575704 1101 102-103 0.049872641361108805 1101 104-105 0.0706713089201898 1101 106-107 0.1427067339810506 1101 108-109 0.06967960519676097 1101 110-111 0.03826092902022893 1101 112-113 0.12932709606399584 1101 114-115 0.08630796778554384 1101 116-117 0.06404831535087396 1101 118-119 0.023901854977978587 1101 120-121 0.06132067852550449 1101 122-123 -0.0664108207389269 1101 124-125 -0.12173574926826447 1102 1 -0.01976989299725318 1102 2 0.08028359950994357 1102 3 0.09926512412139488 1102 4 -0.017921730064706765 1102 5 -0.09753086585970294 1102 6 0.05828103640902782 1102 7 0.08441898071759368 1102 8 0.16570821760706167 1102 9 0.18642982125542318 1102 10-11 0.18530861370521023 1102 12-13 0.13587777474018736 1102 14-15 0.2753722764829618 1102 16-17 0.23393786057111043 1102 18-19 0.16013432023638785 1102 20-21 -0.13001476613143126 1102 22-23 0.12238306873039306 1102 24-25 0.14817657609442847 1102 26-27 0.2844474958893102 1102 28-29 0.2915006413474188 1102 30-31 0.35063972929638965 1102 32-33 0.30883002543117044 1102 34-35 0.4469496388157026 1102 36-37 0.3971914553843092 1102 38-39 0.3682109452743134 1102 40-41 0.417487222625347 1102 42-43 0.31632713904824783 1102 44-45 0.447568147102956 1102 46-47 0.4787392971530551 1102 48-49 0.49807811422380155 1102 50-51 0.5127661824475354 1102 52-53 0.4315656014352598 1102 54-55 0.38537654668954247 1102 56-57 0.4676551736904031 1102 58-59 0.34457032809812205 1102 60-61 0.3808706448629877 1102 62-63 0.5187768481671284 1102 64-65 0.4386715208371683 1102 66-67 0.47966540841812133 1102 68-69 0.608514877311805 1102 70-71 0.46705957951455446 1102 72-73 0.340223660859337 1102 74-75 0.34855127197479874 1102 76-77 0.33791298582373486 1102 78-79 0.3627799945603485 1102 80-81 0.3246156500086954 1102 82-83 0.18053519002101837 1102 84-85 0.31327199575857634 1102 86-87 0.2595020105191779 1102 88-89 0.24508003107964882 1102 90-91 0.18373047792264785 1102 92-93 0.2615871773025056 1102 94-95 0.30442288517935623 1102 96-97 0.11844101072291835 1102 98-99 0.13422080191314123 1102 100-101 0.20745660382035425 1102 102-103 0.21494577523067093 1102 104-105 0.25752038637585883 1102 106-107 0.3195737792775759 1102 108-109 0.2664873858939565 1102 110-111 0.3037124487848537 1102 112-113 0.3253865216785954 1102 114-115 0.24416606209658198 1102 116-117 0.24242487884578168 1102 118-119 0.3731700588525939 1102 120-121 0.32207256496582204 1102 122-123 0.41552344843469413 1102 124-125 0.2171286722111745 1103 1 -0.17339584719572443 1103 2 -0.05902937758929383 1103 3 0.007662070686279776 1103 4 -0.11334157739295136 1103 5 0.01983554635403806 1103 6 0.10026576923345232 1103 7 0.026212873847363483 1103 8 -0.09478796559904623 1103 9 -0.10937170546213082 1103 10-11 -0.08854634812684736 1103 12-13 -0.012500087855229935 1103 14-15 -0.00134528076894469 1103 16-17 0.09891877660164283 1103 18-19 0.09811141947302815 1103 20-21 0.13238981402123784 1103 22-23 -0.040784870200901935 1103 24-25 -0.048388309401758534 1103 26-27 0.08549711420992168 1103 28-29 0.045317435240541215 1103 30-31 -0.005276301237962855 1103 32-33 0.11178804069835024 1103 34-35 0.0991442953042565 1103 36-37 0.21350824927744583 1103 38-39 0.08195140328958672 1103 40-41 0.13456737529709528 1103 42-43 0.07491492530778743 1103 44-45 0.1250490631334813 1103 46-47 -0.08566909979351323 1103 48-49 -0.02100585524184595 1103 50-51 -0.07072618396468044 1103 52-53 -0.1571748565800064 1103 54-55 -0.006799025829536731 1103 56-57 -0.0738524598973882 1103 58-59 -0.021841885642338355 1103 60-61 0.010164537992764622 1103 62-63 -0.036566663283252865 1103 64-65 -0.05846588374298989 1103 66-67 -0.12672772135287147 1103 68-69 -0.14291642039811592 1103 70-71 -0.14124194720300665 1103 72-73 -0.15500534677424582 1103 74-75 -0.21347162878855386 1103 76-77 -0.20264044929076874 1103 78-79 -0.2083077916991911 1103 80-81 -0.2817774797622974 1103 82-83 -0.21164038249806794 1103 84-85 -0.30743411111165386 1103 86-87 -0.23238730245791572 1103 88-89 -0.16331691548523608 1103 90-91 -0.1788649419246795 1103 92-93 -0.19070289903337212 1103 94-95 -0.21704658046949987 1103 96-97 -0.23270402744502405 1103 98-99 -0.31663797671281557 1103 100-101 -0.26200904503460976 1103 102-103 -0.26406185835711327 1103 104-105 -0.20669717087605477 1103 106-107 -0.2114376711041004 1103 108-109 -0.10803551486940322 1103 110-111 -0.1228142687723981 1103 112-113 -0.1860638600007931 1103 114-115 -0.06308584630036407 1103 116-117 -0.09345298374963917 1103 118-119 -0.00469253656725499 1103 120-121 0.02817943519482924 1103 122-123 0.04672573851103223 1103 124-125 -0.08726064076592266 1104 1 -0.2702660037784277 1104 2 -0.15979961673641796 1104 3 -0.028405545229823304 1104 4 -0.2671703213997887 1104 5 0.02393298685695555 1104 6 0.09645259658690009 1104 7 0.0608989841939902 1104 8 0.11358942623432 1104 9 -0.055433237683985226 1104 10-11 -0.08687423751948842 1104 12-13 -0.11915575800311728 1104 14-15 -0.1735418927306469 1104 16-17 -0.08979886756018374 1104 18-19 -0.053235865316288766 1104 20-21 -0.03099087314650717 1104 22-23 0.03904822654431683 1104 24-25 0.07716664663630723 1104 26-27 0.06682687216579808 1104 28-29 0.16902012365400054 1104 30-31 0.11715351900335946 1104 32-33 0.21484600237106832 1104 34-35 0.17197299585613024 1104 36-37 0.15972006323227816 1104 38-39 0.07692963959765109 1104 40-41 0.05582155783689302 1104 42-43 0.15111047986548698 1104 44-45 0.007680840296451663 1104 46-47 0.020639835994543887 1104 48-49 0.041687881450073405 1104 50-51 0.09512509868085317 1104 52-53 0.054037971323872114 1104 54-55 0.17342226854883336 1104 56-57 -0.008828451032570683 1104 58-59 0.13403738923924635 1104 60-61 0.008377452806556107 1104 62-63 0.10112671128401018 1104 64-65 -0.057803195619229086 1104 66-67 0.024161475744335803 1104 68-69 -0.1374501486887496 1104 70-71 -0.018861919102128866 1104 72-73 0.0654000155676826 1104 74-75 -0.05138372664912083 1104 76-77 -0.06469630339799437 1104 78-79 -0.10678976513372618 1104 80-81 -0.12010058910945531 1104 82-83 -0.052406022662033536 1104 84-85 -0.012023679024515843 1104 86-87 -0.06479872676661813 1104 88-89 -0.10466177403934651 1104 90-91 -0.08890140787772083 1104 92-93 -0.05905157438137465 1104 94-95 -0.044686107100147865 1104 96-97 -0.08195243771777427 1104 98-99 -0.07112198528792746 1104 100-101 -0.23111437985506456 1104 102-103 -0.24387250337355582 1104 104-105 -0.2451738210781187 1104 106-107 -0.3060744238598687 1104 108-109 -0.20606990222690413 1104 110-111 -0.16588990212842702 1104 112-113 -0.12462488524812443 1104 114-115 -0.19039258037050288 1104 116-117 -0.11103232526215834 1104 118-119 -0.31692094307391017 1104 120-121 -0.23726608677165117 1104 122-123 -0.262665421301822 1104 124-125 -0.39110405082140076 1105 1 -0.08550905287929211 1105 2 0.02673736762384138 1105 3 0.05810583470823261 1105 4 -0.1559489203117792 1105 5 -0.23973994997027148 1105 6 0.007128237662008985 1105 7 -0.03709405000019217 1105 8 0.06839937477995761 1105 9 0.08578216565581442 1105 10-11 -0.05236000251299089 1105 12-13 -0.07289944019963457 1105 14-15 0.056597142789357235 1105 16-17 0.02084029598191961 1105 18-19 -0.05818576132024589 1105 20-21 -0.09187240183574374 1105 22-23 -0.06883495415625873 1105 24-25 -0.10624607209736325 1105 26-27 -0.041900728349098415 1105 28-29 0.010506828645461042 1105 30-31 0.10384185005100477 1105 32-33 0.06529776258741293 1105 34-35 -0.001304054514434938 1105 36-37 0.09019441145943574 1105 38-39 0.03482264117249656 1105 40-41 0.10377022272410841 1105 42-43 0.10549778007207777 1105 44-45 -0.05394163828176346 1105 46-47 0.09789328820721721 1105 48-49 0.038250575456181934 1105 50-51 -0.012431888695815019 1105 52-53 0.12332774674151636 1105 54-55 0.25512492755054694 1105 56-57 0.08514328589426867 1105 58-59 -0.04462535843092752 1105 60-61 0.11721290756564429 1105 62-63 0.14710243267983714 1105 64-65 0.021901967705417746 1105 66-67 0.08603649108945888 1105 68-69 0.083807133664898 1105 70-71 0.11794022886336819 1105 72-73 0.18332591707132906 1105 74-75 0.1501423670630615 1105 76-77 0.09639935542483613 1105 78-79 0.05185856689600854 1105 80-81 0.12269772871508167 1105 82-83 0.21693671532756298 1105 84-85 0.08741004235027106 1105 86-87 0.0680908550395074 1105 88-89 0.03399757139938586 1105 90-91 0.07682409167157189 1105 92-93 0.03247283575236537 1105 94-95 0.10977256981764327 1105 96-97 0.06371217241105143 1105 98-99 0.008887449766280753 1105 100-101 0.13804351525097758 1105 102-103 0.13034752629951285 1105 104-105 0.0906150231102032 1105 106-107 0.010619834786883331 1105 108-109 0.10635753729579989 1105 110-111 0.0919641666948614 1105 112-113 -0.03160805297111935 1105 114-115 0.02946435053614138 1105 116-117 0.14068545087101114 1105 118-119 0.12469483626507127 1105 120-121 0.13700480100030887 1105 122-123 0.14849074112141025 1105 124-125 0.009699857886555918 1106 1 -0.2368219810080312 1106 2 0.034341749487492024 1106 3 -0.09108343896796356 1106 4 -0.18065466675083286 1106 5 -0.13139789549598646 1106 6 -0.13208155901082108 1106 7 -0.08402511447773975 1106 8 -0.23614966294974238 1106 9 -0.09833527753217908 1106 10-11 -0.1497448494739544 1106 12-13 -0.11063168305055626 1106 14-15 -0.13397536047257574 1106 16-17 -0.1174316948846652 1106 18-19 -0.06883121635993206 1106 20-21 -0.2263923200155773 1106 22-23 -0.18159930666251256 1106 24-25 -0.21683240459708486 1106 26-27 -0.13465261075639745 1106 28-29 -0.19437230993233356 1106 30-31 -0.08468175139511658 1106 32-33 -0.23672242742019733 1106 34-35 -0.3028995136315302 1106 36-37 -0.24937623657796593 1106 38-39 -0.16831170290708997 1106 40-41 -0.28208759776082815 1106 42-43 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-0.7707701360397046 2216 100-101 -0.7831515854628996 2216 102-103 -0.9940110469013668 2216 104-105 -0.8632742251120931 2216 106-107 -0.8525911830086201 2216 108-109 -0.7711348175665478 2216 110-111 -0.739433681370091 2216 112-113 -0.7213441745095643 2216 114-115 -0.742211451640987 2216 116-117 -0.6694787084037692 2216 118-119 -0.7316243111183844 2216 120-121 -0.8776204298033718 2216 122-123 -0.9594239132503901 2216 124-125 -1.0007286696984181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15482.0 3 2537.0 4 1611.0 5 1262.0 6 1289.0 7 1469.0 8 1563.0 9 1741.0 10 2071.0 11 2303.0 12 2628.0 13 2675.0 14 2708.0 15 2744.0 16 2516.0 17 2343.0 18 2054.0 19 1853.0 20 1789.0 21 1648.0 22 1856.0 23 2394.0 24 3982.0 25 6549.0 26 7949.0 27 6528.0 28 6766.0 29 8277.0 30 9023.0 31 10777.0 32 14042.0 33 19121.0 34 28712.0 35 51496.0 36 126180.0 37 305087.0 38 168794.0 39 1023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9859157805966 18.310806671143567 13.617388316729153 24.08588923153068 2 15.228994914966743 19.147249426475135 41.291023418581126 24.332732239977 3 19.963537794709136 23.653834836778785 27.622882796108293 28.75974457240378 4 12.784686119542027 16.05577425849686 36.299546233133995 34.859993388827114 5 14.462651111735614 37.599530181514226 33.18081046090731 14.75700824584285 6 33.178783605557065 35.46579926632922 17.079466587229707 14.275950540884008 7 29.898921954295666 32.02618539983784 19.753400798774813 18.32149184709168 8 25.736914848867254 37.06411874286401 18.93972717985698 18.259239228411754 9 25.91034377475475 18.22250216669812 19.224292021622507 36.642862036924626 10-11 25.56534410173521 27.29100332698449 26.13920237664059 21.004450194639706 12-13 25.676790752478173 24.09920378971593 26.89212545746959 23.33188000033631 14-15 22.69680097653705 25.773457093673464 24.45005244743698 27.079689482352503 16-17 23.195670437778457 28.9046440584121 25.023795897557232 22.875889606252212 18-19 23.208017490960202 27.833691721826458 26.384140408202487 22.57415037901085 20-21 23.909027972839755 27.123315190815223 26.336765154335705 22.630891682009313 22-23 23.59997043175053 26.814255502128375 26.301498785718536 23.28427528040256 24-25 23.371628173563224 27.027241365637018 26.206011100336806 23.395119360462953 26-27 23.24050262003935 27.32388513978046 26.16490726435708 23.270704975823104 28-29 23.086769615024377 27.35608444006821 26.34153077643555 23.215615168471864 30-31 23.32063083027779 27.102442374925772 26.19679613533299 23.380130659463443 32-33 23.208959346000885 27.113993544544563 26.3558137524256 23.32123335702895 34-35 23.54374382331715 27.23798556382382 26.094511202986663 23.123759409872367 36-37 23.57327861037631 27.060846468724222 26.009755031514132 23.35611988938534 38-39 23.232326871153266 27.0786539116213 26.278851805753938 23.410167411471495 40-41 23.521002793007632 26.914939348746508 26.101713028672158 23.462344829573702 42-43 23.268986792657877 26.924076845992793 26.120180388865172 23.686755972484153 44-45 23.29240300135754 27.095615585108014 26.24987016012484 23.36211125340961 46-47 23.410422152102054 27.238703130488563 26.02938041725837 23.321494300151016 48-49 23.54108184544808 27.08600662305339 26.115065795201737 23.257845736296794 50-51 23.36156694439958 27.142092897726762 26.228318189814647 23.268021968059013 52-53 23.492195587066217 27.054737767402315 26.19263774506625 23.260428900465214 54-55 23.39680736470088 26.903890701536753 26.170518860277475 23.52878307348489 56-57 23.2962658292453 27.250800093669504 26.236647592513645 23.21628648457155 58-59 23.494973889202793 26.948792574530007 26.128930955450425 23.427302580816775 60-61 23.419346045610734 27.0177222972401 26.343281924241484 23.219649732907683 62-63 23.246739911695247 27.147557087948677 26.427536479903253 23.178166520452827 64-65 23.226517412636547 26.93376652233706 26.30674345562315 23.532972609403245 66-67 23.21418276852715 27.23536420150465 26.087416047733893 23.463036982234307 68-69 23.164761303062114 27.009039567023237 26.43514837333436 23.391050756580288 70-71 23.186800978817313 26.817252405758808 26.422963174250498 23.572983441173378 72-73 23.22078982605093 26.992908738283806 26.267270314573416 23.51903112109185 74-75 23.18010181811195 27.292453583537622 26.048517008812265 23.478927589538156 76-77 23.437494371052985 26.999371959618085 26.131283255818982 23.431850413509952 78-79 23.322688502381574 26.948256273404414 26.351060840072925 23.377994384141086 80-81 23.271761282504865 27.194562636681667 26.24480144304615 23.28887463776731 82-83 23.326818166806277 27.155334180004743 26.2928101984874 23.225037454701578 84-85 23.48094463225272 27.11008100107481 26.111708228003916 23.297266138668558 86-87 23.38971142384454 27.077697275776234 26.2931280382166 23.239463262162623 88-89 23.317407586228434 27.066143495064942 26.298380762273993 23.318068156432634 90-91 23.37211641530225 27.152581151722423 26.355341105963387 23.119961327011943 92-93 23.22512569574064 26.967952090284736 26.38931928519781 23.41760292877682 94-95 23.217179975751257 27.05950670203292 26.394678007567673 23.32863531464815 96-97 23.378274423093206 26.950061163199752 26.355063634349158 23.316600779357884 98-99 23.203793120015373 27.049642067838953 26.169164985106182 23.577399827039493 100-101 22.976564489088087 27.015148894877672 26.42842045088799 23.579866165146253 102-103 23.062344163611993 26.78145913303323 26.491465330457213 23.66473137289756 104-105 23.062561889028803 26.927800086351894 26.414252506637027 23.595385517982272 106-107 23.0067364473813 27.03575295729259 26.294905895727698 23.662604699598408 108-109 23.097995791739127 26.931068110414003 26.24752596535391 23.723410132492962 110-111 22.951635731060485 27.173233589997487 26.33862974610005 23.53650093284198 112-113 23.037437111393753 27.08903068975995 26.416157132339606 23.457375066506692 114-115 22.92077338253687 27.198923062235547 26.383053841484323 23.497249713743265 116-117 22.820504968810962 27.050493878974883 26.58930800503636 23.539693147177797 118-119 22.82424355980898 27.27856888914512 26.337822970414997 23.559364580630906 120-121 22.875365249943254 27.33312596304085 26.433226965984467 23.358281821031433 122-123 22.848762151808284 27.251694798215432 26.37057986417596 23.528963185800322 124-125 22.901557266503097 27.26609477624577 26.319088914429006 23.513259042822128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 597.0 1 590.0 2 533.0 3 426.0 4 330.5 5 272.5 6 237.0 7 208.0 8 201.0 9 203.0 10 194.5 11 184.5 12 181.0 13 181.5 14 189.0 15 217.5 16 241.0 17 268.5 18 336.0 19 423.5 20 548.0 21 766.0 22 1075.5 23 1433.5 24 1945.5 25 2657.0 26 3549.5 27 4619.5 28 5608.5 29 6758.5 30 8225.5 31 9839.0 32 11418.5 33 12557.5 34 13702.0 35 15057.0 36 16850.0 37 18614.5 38 19387.0 39 20090.5 40 21183.0 41 22576.0 42 24531.0 43 26380.0 44 28169.5 45 30062.5 46 30809.0 47 31162.0 48 32210.5 49 32355.5 50 31604.0 51 31527.5 52 31770.5 53 31963.0 54 32322.0 55 30856.5 56 27506.0 57 24851.5 58 23644.0 59 21609.5 60 18618.0 61 15637.0 62 12269.5 63 9486.0 64 7248.0 65 5350.0 66 3955.5 67 3127.5 68 2341.5 69 1659.0 70 1327.0 71 1019.5 72 770.5 73 688.0 74 570.0 75 380.5 76 265.5 77 180.5 78 118.5 79 77.0 80 52.0 81 31.0 82 23.5 83 21.0 84 15.0 85 16.0 86 16.0 87 10.0 88 8.0 89 8.0 90 5.0 91 4.0 92 4.5 93 3.5 94 2.5 95 2.5 96 3.0 97 2.0 98 2.0 99 2.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05054980416453541 2 0.1895917833154428 3 0.02389408795425783 4 0.11010491785957001 5 0.02161274287319804 6 0.03794237082183655 7 0.038062441615576544 8 0.09509606864207136 9 0.11178590897192986 10-11 0.04880877765530557 12-13 0.032779326691017026 14-15 0.012427327152088871 16-17 0.02887702589446738 18-19 0.049469167020875504 20-21 0.18652997807507307 22-23 0.10428148436318052 24-25 0.07384353815009329 26-27 0.015068884614368632 28-29 0.007444389211879325 30-31 0.007984707783709275 32-33 0.006964106036919367 34-35 0.008765167943019205 36-37 0.004922902543339553 38-39 0.008825203339889199 40-41 0.005763398099519477 42-43 0.010266052864769068 44-45 0.010266052864769068 46-47 0.017350229695428425 48-49 0.017350229695428425 50-51 0.010506194452249045 52-53 0.012967645723918822 54-55 0.013567999692618769 56-57 0.015248990804978614 58-59 0.01686994652046847 60-61 0.009305486514849155 62-63 0.01891115001404828 64-65 0.0308581939911772 66-67 0.027135999385237537 68-69 0.033619822247196944 70-71 0.02455447731982777 72-73 0.015248990804978614 74-75 0.009905840483549101 76-77 0.011166583817818985 78-79 0.024014158747997817 80-81 0.019091256204658267 82-83 0.02035199953892815 84-85 0.016449698742378508 86-87 0.026355539225927607 88-89 0.02737614097271751 90-91 0.026355539225927607 92-93 0.03313953907223699 94-95 0.026595680813407584 96-97 0.02803653033828745 98-99 0.033859963834676925 100-101 0.0208923181107581 102-103 0.028576848910117403 104-105 0.024314335732347793 106-107 0.03379992843780693 108-109 0.023293733985557886 110-111 0.02059214112640813 112-113 0.026295503829057612 114-115 0.013267822708268795 116-117 0.011706902389648938 118-119 0.030978264784917185 120-121 0.023173663191817895 122-123 0.017650406679778397 124-125 0.019811680967098202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 832842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.317393115765 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04412349093715 51.48229357173445 2 11.881757534098815 15.284073404536718 3 3.5463301034826125 6.842721221518882 4 1.5438592579383226 3.9718801123292935 5 0.7968446507064744 2.562548532584138 6 0.4912801825339214 1.8958716378011404 7 0.3326238529980306 1.4975449379068335 8 0.2303724868527685 1.1853566239972735 9 0.16847604842349065 0.9752346213339878 >10 0.9011825622385478 10.325920293684765 >50 0.04540185996679916 1.9777229592361718 >100 0.017187507629095343 1.7838690202405243 >500 5.604621940361222E-4 0.2149630630958599 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.003539686999455E-5 0.0 18-19 0.0 0.0 0.0 1.200707937399891E-4 0.0 20-21 0.0 0.0 0.0 1.200707937399891E-4 0.0 22-23 0.0 0.0 0.0 1.200707937399891E-4 0.0 24-25 0.0 0.0 0.0 1.200707937399891E-4 0.0 26-27 0.0 0.0 0.0 2.401415874799782E-4 0.0 28-29 0.0 0.0 0.0 4.202477780899619E-4 0.0 30-31 0.0 0.0 0.0 0.0021612742873198037 0.0 32-33 0.0 0.0 0.0 0.00540318571829951 0.0 34-35 0.0 0.0 0.0 0.009425557308589145 0.0 36-37 0.0 0.0 0.0 0.01596941556741855 0.0 38-39 0.0 0.0 0.0 0.028816990497597388 0.0 40-41 0.0 0.0 0.0 0.046167220193025806 0.0 42-43 0.0 0.0 0.0 0.06375759147593421 0.0 44-45 0.0 0.0 0.0 0.08182824593380257 0.0 46-47 0.0 0.0 0.0 0.10410137817257055 0.0 48-49 0.0 0.0 0.0 0.12505373168019865 0.0 50-51 0.0 0.0 0.0 0.15008849217498638 0.0 52-53 0.0 0.0 0.0 0.17656410219465396 0.0 54-55 0.0 0.0 0.0 0.20724218999522118 0.0 56-57 0.0 0.0 0.0 0.24482434843583778 0.0 58-59 0.0 0.0 0.0 0.2816260467171444 0.0 60-61 0.0 0.0 0.0 0.3325960986597698 0.0 62-63 0.0 0.0 0.0 0.3803842745682855 0.0 64-65 0.0 0.0 0.0 0.43075397254221093 0.0 66-67 0.0 0.0 0.0 0.48628671464695583 0.0 68-69 0.0 0.0 0.0 0.5538865715225697 0.0 70-71 0.0 0.0 0.0 0.6300114547537228 0.0 72-73 0.0 0.0 0.0 0.7083576476690656 0.0 74-75 0.0 0.0 0.0 0.7906061413809582 0.0 76-77 0.0 0.0 0.0 0.88167983843274 0.0 78-79 0.0 0.0 0.0 0.9890831634331602 0.0 80-81 0.0 0.0 0.0 1.1116154084448189 0.0 82-83 0.0 0.0 0.0 1.235048184409528 0.0 84-85 0.0 0.0 0.0 1.356379721483787 0.0 86-87 0.0 0.0 0.0 1.4913993290444045 0.0 88-89 0.0 0.0 0.0 1.65331479440278 0.0 90-91 0.0 0.0 0.0 1.8350419407282534 0.0 92-93 0.0 0.0 0.0 2.028175812459026 0.0 94-95 0.0 0.0 0.0 2.212184303865559 0.0 96-97 0.0 0.0 0.0 2.4018361225778717 0.0 98-99 0.0 0.0 0.0 2.6162225248006226 0.0 100-101 0.0 0.0 0.0 2.86260779355508 0.0 102-103 0.0 0.0 0.0 3.130725875976476 0.0 104-105 0.0 0.0 0.0 3.4112712855499603 0.0 106-107 0.0 0.0 0.0 3.6762074919372463 0.0 108-109 0.0 0.0 0.0 3.96671877739115 0.0 110-111 0.0 0.0 0.0 4.29799409731978 0.0 112-113 0.0 0.0 0.0 4.657606124571047 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1600 0.0 37.6668 1 ATCAACG 1705 0.0 34.617996 3 GTACATG 3415 0.0 34.421745 1 GGTATCA 1125 0.0 33.9457 1 TCAACGC 1750 0.0 33.72985 4 CAACGCA 1750 0.0 33.709553 5 TACATGG 3445 0.0 33.08075 2 AACGCAG 1850 0.0 32.209507 6 TATCAAC 1945 0.0 30.370138 2 ACATGGG 3595 0.0 29.851398 3 GAGTACA 2480 0.0 27.910248 1 ACGCAGA 2135 0.0 27.909878 7 CGCAGAG 2240 0.0 26.601603 8 AGTACAT 2510 0.0 26.148705 2 GTGTAGC 675 0.0 25.63608 1 CATGGGG 2110 0.0 24.866663 4 CATGGGA 1685 0.0 24.061699 4 CGGTTAC 125 2.0203515E-8 23.788511 28-29 CTGTTCG 185 2.6688456E-5 22.535807 9 ATTCGCG 95 3.313744E-5 21.915075 80-81 >>END_MODULE