FastQCFastQC Report
Fri 27 May 2016
SRR940086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832842
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37220.4469034943002394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.34148133739652897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.19835695125846198No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.10266052864769068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.054.409061
GTATCAA35000.051.106661
ATCAACG42000.041.868993
TCAACGC43050.040.8477974
CAACGCA43600.040.2792055
TATCAAC44450.039.695362
AACGCAG44750.039.222896
ACGCAGA51550.034.0448767
CGCAGAG52350.033.5246168
GTACATG33700.031.4930271
TACATGG34100.030.5916862
GCAGAGT57500.030.5219759
GTGGTAT11150.030.4806541
ACATGGG34600.029.2881953
CCTATAC2502.1827873E-1128.6128443
GAGTACT37850.027.506212-13
CATGGGG20600.026.9113524
TGGTATC11750.026.8879552
GTACTTT38850.026.49192614-15
AGAGTAC53500.025.35359610-11
Produced by FastQC (version 0.11.5)