##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832842 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.120408192670396 34.0 33.0 34.0 31.0 34.0 2 33.17746223173183 34.0 33.0 34.0 31.0 34.0 3 33.22943127267837 34.0 34.0 34.0 31.0 34.0 4 36.460204936830756 37.0 37.0 37.0 35.0 37.0 5 36.45860079102639 37.0 37.0 37.0 35.0 37.0 6 36.53722674889114 37.0 37.0 37.0 35.0 37.0 7 36.526158623124196 37.0 37.0 37.0 35.0 37.0 8 36.530495580194085 37.0 37.0 37.0 35.0 37.0 9 38.40345587758542 39.0 39.0 39.0 37.0 39.0 10-11 38.37964103635504 39.0 39.0 39.0 37.0 39.0 12-13 38.35736190057658 39.0 39.0 39.0 37.0 39.0 14-15 39.97388520271552 41.0 40.0 41.0 38.0 41.0 16-17 39.98314446197478 41.0 40.0 41.0 38.0 41.0 18-19 40.01377812358166 41.0 40.0 41.0 38.0 41.0 20-21 40.00668854356529 41.0 40.0 41.0 38.0 41.0 22-23 39.9670801904803 41.0 40.0 41.0 38.0 41.0 24-25 39.94024977126514 41.0 40.0 41.0 38.0 41.0 26-27 39.849060806251366 41.0 40.0 41.0 37.5 41.0 28-29 39.77213805259581 41.0 40.0 41.0 37.0 41.0 30-31 39.64402671815302 41.0 40.0 41.0 37.0 41.0 32-33 39.48564853837823 41.0 40.0 41.0 35.5 41.0 34-35 39.381524947108815 41.0 40.0 41.0 35.0 41.0 36-37 39.2848493471751 41.0 40.0 41.0 35.0 41.0 38-39 39.170929780198406 41.0 39.0 41.0 35.0 41.0 40-41 39.046302299835986 41.0 39.0 41.0 35.0 41.0 42-43 38.889238294898675 41.0 39.0 41.0 35.0 41.0 44-45 38.70080999757457 41.0 39.0 41.0 35.0 41.0 46-47 38.43591221384128 41.0 39.0 41.0 35.0 41.0 48-49 38.181502013587206 41.0 38.0 41.0 34.5 41.0 50-51 38.19664654280164 41.0 39.0 41.0 35.0 41.0 52-53 38.09385633769671 41.0 38.0 41.0 34.5 41.0 54-55 37.93155184296661 41.0 38.0 41.0 34.0 41.0 56-57 37.735716978730665 40.5 37.0 41.0 34.0 41.0 58-59 37.51042454631251 40.0 37.0 41.0 34.0 41.0 60-61 37.26932959673023 40.0 36.0 41.0 33.5 41.0 62-63 37.0011220615675 39.0 35.5 41.0 33.0 41.0 64-65 36.6878375490189 39.0 35.0 41.0 33.0 41.0 66-67 36.36453492979461 38.5 35.0 41.0 33.0 41.0 68-69 36.01312253704785 37.0 35.0 40.5 32.0 41.0 70-71 35.65161939479517 37.0 35.0 39.5 32.0 41.0 72-73 35.29475638836658 36.0 35.0 39.0 32.0 41.0 74-75 34.939547357121754 36.0 35.0 39.0 31.5 41.0 76-77 34.58570953434145 35.0 35.0 37.5 31.0 39.5 78-79 34.267548946859065 35.0 35.0 37.0 31.0 39.0 80-81 33.965311547688515 35.0 35.0 36.5 31.0 39.0 82-83 33.69946280326881 35.0 35.0 36.0 31.0 38.0 84-85 33.46252650562772 35.0 35.0 36.0 31.0 37.0 86-87 33.2630715069605 35.0 35.0 35.5 30.0 37.0 88-89 33.16790939938188 35.0 35.0 35.0 30.5 36.0 90-91 33.16704248825107 35.0 35.0 35.0 31.0 36.0 92-93 33.09031665069725 35.0 35.0 35.0 31.0 36.0 94-95 33.01636564918676 35.0 35.0 35.0 31.0 36.0 96-97 32.95020063829634 35.0 35.0 35.0 31.0 36.0 98-99 32.9007807003009 35.0 35.0 35.0 31.0 35.5 100-101 32.84170166730304 35.0 35.0 35.0 31.0 35.0 102-103 32.36450971492792 35.0 33.5 35.0 29.5 35.0 104-105 32.7565156416223 35.0 34.5 35.0 30.5 35.0 106-107 32.801854373338514 35.0 35.0 35.0 31.0 35.0 108-109 32.79070519978579 35.0 35.0 35.0 31.0 35.0 110-111 32.75312964523883 35.0 35.0 35.0 31.0 35.0 112-113 32.7231893924658 35.0 35.0 35.0 31.0 35.0 114-115 32.665432338907024 35.0 35.0 35.0 30.5 35.0 116-117 32.61004128033889 35.0 35.0 35.0 30.0 35.0 118-119 32.55167967033363 35.0 35.0 35.0 30.0 35.0 120-121 32.48017391053766 35.0 35.0 35.0 30.0 35.0 122-123 32.381147924816474 35.0 35.0 35.0 30.0 35.0 124-125 31.318490181811196 34.5 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10561332189021044 1101 2 0.10057173479057724 1101 3 0.08812831149786149 1101 4 -0.06410667064531594 1101 5 -0.035673893735364004 1101 6 0.039216534254897795 1101 7 0.09268874427846185 1101 8 0.11529507786519844 1101 9 0.09081808572534555 1101 10-11 0.08106270616595168 1101 12-13 0.07720237648391759 1101 14-15 0.14200874134930785 1101 16-17 0.10683824128700792 1101 18-19 0.11480354509274093 1101 20-21 0.12259392726946317 1101 22-23 0.13886298321428825 1101 24-25 0.09433921416663793 1101 26-27 0.1734631777204072 1101 28-29 0.1894591663322629 1101 30-31 0.19705225215996336 1101 32-33 0.1549859479080311 1101 34-35 0.156842103084891 1101 36-37 0.14625702286821962 1101 38-39 0.14674937097687035 1101 40-41 0.0970646971222422 1101 42-43 0.08982524036328243 1101 44-45 4.4258902961047397E-4 1101 46-47 0.07287372125356484 1101 48-49 0.11482309977238714 1101 50-51 0.08330150225665278 1101 52-53 0.02800703901213808 1101 54-55 0.08688835854361798 1101 56-57 0.13303349086488225 1101 58-59 0.0607630096034697 1101 60-61 0.042413685920486444 1101 62-63 -0.08819583315999324 1101 64-65 -0.09561185569245367 1101 66-67 -0.022841649758454707 1101 68-69 0.012547051250876962 1101 70-71 0.049884179141741924 1101 72-73 -0.04547178036084176 1101 74-75 -0.04621353396039041 1101 76-77 -0.025260428899720466 1101 78-79 -0.023651171294794437 1101 80-81 -0.04264721155443141 1101 82-83 -0.003105557492006028 1101 84-85 -0.014060122043439094 1101 86-87 0.04679111882836651 1101 88-89 0.08354824887891255 1101 90-91 0.012954669552485143 1101 92-93 -0.020128959374737576 1101 94-95 0.10956446832547329 1101 96-97 0.10251494869493882 1101 98-99 0.05527362327529772 1101 100-101 0.06387852654732029 1101 102-103 0.06892603528164898 1101 104-105 0.12283659087513854 1101 106-107 0.029169282660014062 1101 108-109 -0.001078562149260165 1101 110-111 -0.01782324277149172 1101 112-113 -0.04000174663902811 1101 114-115 -0.0634053019795715 1101 116-117 -0.14972542212791495 1101 118-119 -0.16160680179234532 1101 120-121 -0.22425514236589095 1101 122-123 -0.18239153095836258 1101 124-125 -0.4255363032545034 1102 1 0.19605450374232447 1102 2 0.17455825342077702 1102 3 0.11134104195097194 1102 4 0.0036377615196769852 1102 5 0.02140968051097758 1102 6 0.10131628321745012 1102 7 0.12881471677413003 1102 8 0.14647166038918158 1102 9 0.18852508183901762 1102 10-11 0.20660841986614997 1102 12-13 0.15949444721353245 1102 14-15 0.21527069124428522 1102 16-17 0.2523182863712776 1102 18-19 0.24146858940854088 1102 20-21 0.2740887162296701 1102 22-23 0.21649430214895204 1102 24-25 0.19425559600704645 1102 26-27 0.21949021730820562 1102 28-29 0.22338581655585443 1102 30-31 0.11371403957589621 1102 32-33 0.19761062496262127 1102 34-35 0.2872699645824781 1102 36-37 0.21279795734231755 1102 38-39 0.2515163379614265 1102 40-41 0.18740158498974324 1102 42-43 0.2982901609900779 1102 44-45 0.25410431423509294 1102 46-47 0.31077470417861974 1102 48-49 0.336552568495172 1102 50-51 0.3891079326212079 1102 52-53 0.41185601766297 1102 54-55 0.31946577395570586 1102 56-57 0.27617332531190897 1102 58-59 0.33938347464485474 1102 60-61 0.3160943670150971 1102 62-63 0.3352908062316615 1102 64-65 0.3074834799877948 1102 66-67 0.3165780914460896 1102 68-69 0.3825907998328404 1102 70-71 0.23493510022230168 1102 72-73 0.22856112164672737 1102 74-75 0.279063851444306 1102 76-77 0.34074238520814504 1102 78-79 0.36682367738743693 1102 80-81 0.41034181745028064 1102 82-83 0.3398217550898721 1102 84-85 0.33820378931033446 1102 86-87 0.4335809133875088 1102 88-89 0.4038025981210467 1102 90-91 0.40268573744970126 1102 92-93 0.3367403547733758 1102 94-95 0.3664501584589033 1102 96-97 0.361175008856101 1102 98-99 0.4065330188825058 1102 100-101 0.4621269365214786 1102 102-103 0.36059834358199083 1102 104-105 0.40667495715560875 1102 106-107 0.30180885362749876 1102 108-109 0.3060836900152992 1102 110-111 0.3372936579772272 1102 112-113 0.42508126771328847 1102 114-115 0.41535897897744434 1102 116-117 0.4389944515482824 1102 118-119 0.48853399508688966 1102 120-121 0.4833603312019932 1102 122-123 0.48013966575107503 1102 124-125 0.4655235980870458 1103 1 0.04052015259728847 1103 2 0.09058878777192092 1103 3 0.012104400729597842 1103 4 -0.06697292550322231 1103 5 -0.08259795307680662 1103 6 0.04692697024034942 1103 7 0.004768915247410632 1103 8 -0.007154293809286116 1103 9 0.042532715426801815 1103 10-11 0.027696206125696676 1103 12-13 0.024952462480712256 1103 14-15 0.10553786681680322 1103 16-17 0.16691752301250062 1103 18-19 0.17181210085892218 1103 20-21 0.09326810554264853 1103 22-23 0.08767750825582254 1103 24-25 0.09835864944216155 1103 26-27 0.053936782193702015 1103 28-29 0.11842398449478964 1103 30-31 0.1709659479728387 1103 32-33 0.07451902190918247 1103 34-35 0.03870126229240611 1103 36-37 -0.01048448540576885 1103 38-39 0.11077206315226107 1103 40-41 0.12299318804317494 1103 42-43 0.16231687854732968 1103 44-45 0.09379897072364685 1103 46-47 0.08701516219388594 1103 48-49 0.014510583762344709 1103 50-51 -0.005930235317727295 1103 52-53 0.06500487262481158 1103 54-55 0.03797722433738926 1103 56-57 0.027127523785189567 1103 58-59 0.08938347464485474 1103 60-61 0.11237299296929848 1103 62-63 0.07908851615533052 1103 64-65 0.115689586858025 1103 66-67 0.08661625938501771 1103 68-69 0.09060606700841589 1103 70-71 0.22253052006962548 1103 72-73 0.14172906057802948 1103 74-75 0.1855524010626297 1103 76-77 0.24102864475013064 1103 78-79 0.02569772318896213 1103 80-81 0.19803265714493534 1103 82-83 0.24440190776163462 1103 84-85 0.07913890381415456 1103 86-87 0.07766488285316342 1103 88-89 0.058859850029442384 1103 90-91 0.03722772218252146 1103 92-93 -0.022992469654106173 1103 94-95 -0.008549841541096725 1103 96-97 -0.16172575450268312 1103 98-99 -0.10587156127016328 1103 100-101 -0.19436161309683797 1103 102-103 -0.14655814496762787 1103 104-105 -0.18492809627950635 1103 106-107 -0.20391633721219904 1103 108-109 -0.20870638632057137 1103 110-111 -0.2328399298090318 1103 112-113 -0.15411720556915753 1103 114-115 -0.17672117369431106 1103 116-117 -0.19411623547461687 1103 118-119 -0.18083623392074344 1103 120-121 -0.14211676803465423 1103 122-123 -0.19543285333289617 1103 124-125 -0.1303733484778391 1104 1 0.18561444985817133 1104 2 0.21801321235583515 1104 3 0.22125854995393013 1104 4 0.1053350397907522 1104 5 0.1789483916368937 1104 6 0.18492543483179702 1104 7 0.22275709549857936 1104 8 0.19658609178105024 1104 9 0.2295972605258072 1104 10-11 0.20977067344031752 1104 12-13 0.18871338053914144 1104 14-15 0.2785483539468956 1104 16-17 0.2745980059419537 1104 18-19 0.26503023026778294 1104 20-21 0.15094913165907542 1104 22-23 0.19587130288768861 1104 24-25 0.24174643425320141 1104 26-27 0.30668892959292293 1104 28-29 0.16300877236216138 1104 30-31 0.2107670691107728 1104 32-33 0.24101579175723487 1104 34-35 0.21738714812174464 1104 36-37 0.036216015774755306 1104 38-39 0.07345783310170617 1104 40-41 -0.013295142851852404 1104 42-43 0.09830410278939894 1104 44-45 0.0924183704658148 1104 46-47 -0.027122003383986737 1104 48-49 -0.17652465820935248 1104 50-51 -0.1788737293861189 1104 52-53 -0.20643109649319769 1104 54-55 -0.3088132648205999 1104 56-57 -0.21220066254968373 1104 58-59 -0.1945620907765857 1104 60-61 -0.2054058792296658 1104 62-63 -0.2825119661947184 1104 64-65 -0.17789211570726593 1104 66-67 -0.2784714834195583 1104 68-69 -0.21935724976374615 1104 70-71 -0.10936306597843526 1104 72-73 -0.07193263532417404 1104 74-75 -0.062001446873985344 1104 76-77 -0.1328922237696517 1104 78-79 -0.24596728977510196 1104 80-81 -0.14605185550770017 1104 82-83 -0.07895640010226401 1104 84-85 -0.04406043132497217 1104 86-87 -0.005976859980385996 1104 88-89 -0.07776254086240186 1104 90-91 -0.08318959027311479 1104 92-93 -0.0913833325247353 1104 94-95 -0.10317210931056309 1104 96-97 -0.013645739438295834 1104 98-99 -0.041724799388859424 1104 100-101 -0.08099667835170266 1104 102-103 -0.0796700994268349 1104 104-105 -0.03084677032386196 1104 106-107 -0.07605102585741719 1104 108-109 -0.1168344553270515 1104 110-111 -0.18854665229117984 1104 112-113 -0.17131541087934465 1104 114-115 -0.125227952667899 1104 116-117 -0.14692415007113624 1104 118-119 -0.17321532049750488 1104 120-121 -0.17882987115326898 1104 122-123 -0.17723789990213135 1104 124-125 -0.1454341129954173 1105 1 0.12086507506204214 1105 2 0.09927358126996211 1105 3 0.12689923923900182 1105 4 -0.06284041714295796 1105 5 -0.08371089066764625 1105 6 0.021867354882871837 1105 7 0.021069658851985196 1105 8 0.0025869967044584996 1105 9 0.07667397207620752 1105 10-11 0.14673819791359222 1105 12-13 0.19308805390738826 1105 14-15 0.2703566925706369 1105 16-17 0.21941872237472637 1105 18-19 0.2466064455132937 1105 20-21 0.19632683196107337 1105 22-23 0.22018410474804284 1105 24-25 0.24614741353467195 1105 26-27 0.24873437218114702 1105 28-29 0.24161259478672292 1105 30-31 0.275296351099648 1105 32-33 0.276548942469077 1105 34-35 0.29415218721233316 1105 36-37 0.2620908627620224 1105 38-39 0.38677441249119937 1105 40-41 0.2602346196583625 1105 42-43 0.37057907323908523 1105 44-45 0.3229309499535091 1105 46-47 0.2550343449343657 1105 48-49 0.32859885682184853 1105 50-51 0.4429910441901157 1105 52-53 0.5722169198655678 1105 54-55 0.548977845183714 1105 56-57 0.5253461181326671 1105 58-59 0.5899267151804821 1105 60-61 0.487307733554303 1105 62-63 0.4487257796340387 1105 64-65 0.53531468033772 1105 66-67 0.48783555218461316 1105 68-69 0.3845300627189303 1105 70-71 0.5052263688653227 1105 72-73 0.5453364952127799 1105 74-75 0.48155676109706036 1105 76-77 0.35565768907009243 1105 78-79 0.40102313837994785 1105 80-81 0.4068107539687418 1105 82-83 0.5142928010748307 1105 84-85 0.49272926517493687 1105 86-87 0.3214440732131152 1105 88-89 0.4553478352420157 1105 90-91 0.47017984646467426 1105 92-93 0.5557421326514955 1105 94-95 0.5355223106791343 1105 96-97 0.470990730378297 1105 98-99 0.4369139222139111 1105 100-101 0.44681919428558814 1105 102-103 0.5017782690804324 1105 104-105 0.5157278490295738 1105 106-107 0.4960499748195204 1105 108-109 0.491127643113181 1105 110-111 0.5839028140495301 1105 112-113 0.6110838287043805 1105 114-115 0.5471406668328456 1105 116-117 0.5945737787766276 1105 118-119 0.5557785309337078 1105 120-121 0.5802105943561742 1105 122-123 0.4903395359377498 1105 124-125 0.5315617166404749 1106 1 0.15987915574052636 1106 2 0.12432251026854857 1106 3 0.15700926152888428 1106 4 0.09119248760858767 1106 5 0.10624915065017859 1106 6 0.09870626974640828 1106 7 0.12094257936954733 1106 8 0.10891412783417564 1106 9 0.09869711821082205 1106 10-11 0.15059135655227607 1106 12-13 0.21891084258847826 1106 14-15 0.19854064521823744 1106 16-17 0.1414593674220228 1106 18-19 0.1186681572127597 1106 20-21 0.1496149286230235 1106 22-23 0.18226243191994485 1106 24-25 0.28819152438603624 1106 26-27 0.23332258708818188 1106 28-29 0.2271394649617804 1106 30-31 0.17495112983183958 1106 32-33 0.19178369972687648 1106 34-35 0.1262314318029638 1106 36-37 0.16152732981649365 1106 38-39 0.22961888623263604 1106 40-41 0.22211757062063242 1106 42-43 0.15576378020875126 1106 44-45 0.22703945680357407 1106 46-47 0.2988682717583302 1106 48-49 0.25439327347945806 1106 50-51 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-0.4460405932185125 2216 36-37 -0.420608433155536 2216 38-39 -0.4128612500890583 2216 40-41 -0.5255978081214465 2216 42-43 -0.4199836428816326 2216 44-45 -0.38241134927602616 2216 46-47 -0.26594667193142385 2216 48-49 -0.1695769568517278 2216 50-51 -0.3300006013914043 2216 52-53 -0.3909084743876008 2216 54-55 -0.3429275560473002 2216 56-57 -0.3424667276979747 2216 58-59 -0.3648872473051199 2216 60-61 -0.5182907865030018 2216 62-63 -0.46833834755065595 2216 64-65 -0.5053692483283214 2216 66-67 -0.2947418813884113 2216 68-69 -0.3048121805279038 2216 70-71 -0.37626050169167513 2216 72-73 -0.3925454492663718 2216 74-75 -0.3855965385333562 2216 76-77 -0.2506054756120193 2216 78-79 -0.27201039425846574 2216 80-81 -0.3360601989068215 2216 82-83 -0.30639535628453984 2216 84-85 -0.3310856760082359 2216 86-87 -0.33966664357540566 2216 88-89 -0.4423570239564967 2216 90-91 -0.4073637425663179 2216 92-93 -0.3509859383745564 2216 94-95 -0.35601045569605816 2216 96-97 -0.33009198506263004 2216 98-99 -0.26121135707478516 2216 100-101 -0.3848880581734022 2216 102-103 -0.4549680962926672 2216 104-105 -0.39848715853308647 2216 106-107 -0.35153662092239557 2216 108-109 -0.3482642347965452 2216 110-111 -0.41863615141014066 2216 112-113 -0.4051676495888188 2216 114-115 -0.4461589035998941 2216 116-117 -0.4847592073306686 2216 118-119 -0.4614846347480892 2216 120-121 -0.5361751924357101 2216 122-123 -0.6292112728623316 2216 124-125 -0.527580008566435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 12.0 5 33.0 6 104.0 7 205.0 8 345.0 9 614.0 10 1000.0 11 1535.0 12 2081.0 13 3246.0 14 5353.0 15 5919.0 16 2924.0 17 2261.0 18 2005.0 19 1710.0 20 1463.0 21 1352.0 22 1429.0 23 1589.0 24 1974.0 25 2473.0 26 3098.0 27 3920.0 28 4986.0 29 6057.0 30 7474.0 31 9395.0 32 11965.0 33 16075.0 34 23379.0 35 42713.0 36 106632.0 37 334316.0 38 220965.0 39 2226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5930480416469 18.139699657145602 13.840869925484709 23.42638237572279 2 15.686823083019139 19.050094858432796 41.186570927691456 24.076511130856606 3 20.07936698525527 23.563709716151962 28.44375870515345 27.913164593439316 4 12.941756444786456 16.011446710273184 36.52799153520585 34.51880530973451 5 14.15357007298218 36.95329428778714 33.42021688055234 15.472918758678345 6 32.81400481781873 35.621356914866794 16.810848978435097 14.753789288879387 7 29.448442801875984 32.12337994481546 19.863911762375096 18.564265490933455 8 26.196085211840902 35.94859529178404 18.71975716882674 19.13556232754832 9 26.570706088309663 16.585378739304694 19.51450575259173 37.32940941979391 10-11 25.806155309170286 26.465944320771527 26.021502277742957 21.70639809231523 12-13 26.524118620338548 23.320209595577552 27.257390957708665 22.89828082637523 14-15 23.24240372123404 24.635825282586612 25.738255275310323 26.38351572086902 16-17 22.68389442415248 28.126943645973668 26.0812374976286 23.10792443224525 18-19 22.553197365166504 26.177534274208075 28.896357292259516 22.37291106836591 20-21 23.198757987709552 25.381164734727594 29.31948676939924 22.100590508163613 22-23 22.91539091448318 25.162755960914556 29.238078771243526 22.68377435335874 24-25 22.747832121819027 25.34736480628979 29.18764903787273 22.717154034018456 26-27 22.57955290439243 25.816121185050704 29.04656585522824 22.55776005532862 28-29 22.419932244010415 25.835708235000297 29.23641533232914 22.507944188660147 30-31 22.662276067431918 25.54392200182508 29.090041304452235 22.703760626290766 32-33 22.56507852648768 25.634815810960088 29.102528696988617 22.697576965563616 34-35 22.884836477704233 25.701472376063755 28.94520239542528 22.46848875080673 36-37 22.950445677317255 25.568616055910336 28.784204766018657 22.69673350075375 38-39 22.637749775312535 25.728920953839555 28.920113325332437 22.713215945515465 40-41 22.984311550089934 25.55316614676013 28.72753775626109 22.734984546888846 42-43 22.797981586933748 25.588098447166747 28.568109433769695 23.04581053212981 44-45 22.93201958850217 25.779460649792185 28.455679422887233 22.83284033881841 46-47 23.075274407613954 26.22161893137874 27.94962294655813 22.75348371444918 48-49 23.340071407172776 26.114408771543275 27.762465186451728 22.78305463483222 50-51 23.20189570842016 26.3716959734207 27.6348611989143 22.79154711924484 52-53 23.314746374462384 26.31177342160938 27.438877962446657 22.934602241481578 54-55 23.087332291118845 26.196145247237773 27.561590313648924 23.154932147994458 56-57 23.130041154733995 26.47212974997974 27.523781792857505 22.87404730242876 58-59 23.25169797659665 26.266000423856262 27.49062082935343 22.99168077019366 60-61 23.206397425660544 26.2322068597021 27.71581405680597 22.84558165783138 62-63 23.030192419566028 26.354054162766865 27.80463029279881 22.811123124868296 64-65 22.950195688313155 26.072023145193583 27.827184315637233 23.15059685085603 66-67 22.943139997718635 26.314035793404457 27.61279244988503 23.130031758991876 68-69 22.874079873107892 26.19941440876238 27.817685047491487 23.10882067063825 70-71 22.950260646961603 26.136859616861273 27.686092564942612 23.226787171234516 72-73 23.00448344343825 26.340890589091327 27.482943943749234 23.171682023721186 74-75 22.931041417894544 26.538214700173686 27.24405213243112 23.286691749500655 76-77 23.129890059221047 26.370657483321562 27.287851279760616 23.211601177696767 78-79 23.036528200029778 26.392170847561825 27.389486045271816 23.18181490713658 80-81 23.03528160203256 26.579171079268338 27.247965400400076 23.13758191829903 82-83 23.141544254492448 26.588836778164403 27.223831170858336 23.045787796484806 84-85 23.35040343883579 26.474532923490706 27.056517458335332 23.118546179338168 86-87 23.16124535399678 26.456838322389846 27.259814165874698 23.122102157738674 88-89 23.11837059130063 26.465944320771527 27.218427985140035 23.197257102787805 90-91 23.143206892556258 26.484903171735763 27.34821373360205 23.023676202105925 92-93 22.93732642203355 26.38919406992228 27.299454036134236 23.37402547190994 94-95 22.894044374962853 26.514031299135667 27.277868532103316 23.314055793798165 96-97 23.158830027376208 26.30871475913741 27.25805676960761 23.274398443878777 98-99 22.890716366369613 26.398344463895913 27.068879811536885 23.642059358197592 100-101 22.78939867129181 26.348249781770544 27.2776611085696 23.58469043836804 102-103 22.852113606182208 26.054401675227712 27.39289084844424 23.700593870145838 104-105 22.847130668241995 26.211814485820838 27.264355063745583 23.67669978219158 106-107 22.818395872431527 26.255734901943832 27.12451025948593 23.801358966138714 108-109 22.881982237810185 26.19445713534409 26.973301482343775 23.95025914450195 110-111 22.809308368213898 26.341791120044377 27.126573827928947 23.722326683812774 112-113 22.804819404412484 26.36203887534423 27.08414506241584 23.74899665782745 114-115 22.6893404977036 26.4184552577072 27.04721880347012 23.844985441119082 116-117 22.596132487227365 26.276993642198033 27.200585950398338 23.926287920176264 118-119 22.498946374989647 26.439984198626625 27.081164546809166 23.979904879574562 120-121 22.554018446509268 26.50633584701993 27.184556946978443 23.755088759492363 122-123 22.458176089679014 26.4582985626239 27.116830784415196 23.96669456328189 124-125 22.44304093848121 26.6055701309383 27.003007798663603 23.94838113191689 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 81.0 1 78.0 2 179.0 3 288.5 4 180.0 5 99.0 6 293.0 7 838.5 8 1547.5 9 1730.5 10 1186.0 11 672.0 12 575.0 13 635.5 14 696.0 15 733.5 16 798.5 17 913.5 18 1011.5 19 1074.5 20 1220.5 21 1438.5 22 1691.5 23 2029.5 24 2552.5 25 3266.5 26 4078.0 27 5056.0 28 6071.0 29 7190.0 30 8514.5 31 10076.0 32 11509.5 33 12555.5 34 13738.5 35 15087.0 36 16627.0 37 18114.5 38 19135.0 39 19828.5 40 20692.5 41 22246.5 42 24118.5 43 25650.0 44 27497.5 45 29533.5 46 30177.0 47 30587.5 48 31355.5 49 31580.0 50 31277.5 51 30995.5 52 31380.5 53 31709.5 54 31947.0 55 30493.0 56 27124.5 57 24639.0 58 23351.5 59 21092.5 60 18168.0 61 15223.5 62 11983.5 63 9329.5 64 7090.0 65 5132.0 66 3715.0 67 2934.0 68 2188.5 69 1514.0 70 1203.0 71 941.5 72 703.5 73 607.0 74 458.5 75 341.0 76 260.5 77 117.0 78 59.5 79 36.0 80 15.0 81 9.0 82 7.0 83 2.0 84 0.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015248990804978614 2 0.00264155746227976 3 2.401415874799782E-4 4 0.13952226232586734 5 0.03722194605939662 6 0.012007079373998909 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.003539686999455E-5 30-31 2.401415874799782E-4 32-33 2.401415874799782E-4 34-35 5.403185718299509E-4 36-37 0.0010206017467899074 38-39 0.0022213096841897984 40-41 0.0 42-43 0.0011406725405298964 44-45 7.80460159309929E-4 46-47 3.0017698434997277E-4 48-49 0.0015008849217498636 50-51 0.0016209557154898528 52-53 0.0 54-55 0.0 56-57 0.0011406725405298964 58-59 0.0015008849217498636 60-61 8.404955561799238E-4 62-63 6.003539686999455E-5 64-65 0.0015008849217498636 66-67 8.404955561799238E-4 68-69 0.0010206017467899074 70-71 0.0012607433342698856 72-73 0.0 74-75 1.8010619060998366E-4 76-77 0.003602123812199673 78-79 7.204247624399346E-4 80-81 0.0 82-83 0.0 84-85 2.401415874799782E-4 86-87 3.0017698434997277E-4 88-89 0.0 90-91 1.8010619060998366E-4 92-93 3.602123812199673E-4 94-95 9.005309530499183E-4 96-97 2.401415874799782E-4 98-99 0.0 100-101 3.602123812199673E-4 102-103 0.0 104-105 0.0 106-107 3.602123812199673E-4 108-109 6.6038936556994E-4 110-111 0.0 112-113 6.003539686999455E-5 114-115 0.0020412034935798147 116-117 8.404955561799238E-4 118-119 3.602123812199673E-4 120-121 1.8010619060998366E-4 122-123 6.003539686999455E-4 124-125 8.404955561799238E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 832842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.18576778367115 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22954272410844 47.8653463494008 2 12.854766197274287 15.730574789196197 3 3.9739841899605444 7.294538214687191 4 1.6548516873010177 4.050134842224658 5 0.869640436302907 2.660480889546006 6 0.5585369570202952 2.0504707530505257 7 0.35233898426506677 1.5090691890663854 8 0.2433020899363613 1.190930014090246 9 0.19374212912515548 1.0668834830309215 >10 0.9945348816028269 10.940053846501467 >50 0.05199937073515023 2.162034454569316 >100 0.021386882841326255 2.1880778556292246 >500 7.848397295143622E-4 0.30481343357430934 >1k 5.886297971357717E-4 0.9865918854327194 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3722 0.4469034943002394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.34148133739652897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1652 0.19835695125846198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.10266052864769068 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.003539686999455E-5 0.0 18-19 0.0 0.0 0.0 1.200707937399891E-4 0.0 20-21 0.0 0.0 0.0 1.200707937399891E-4 0.0 22-23 0.0 0.0 0.0 1.200707937399891E-4 0.0 24-25 0.0 0.0 0.0 1.200707937399891E-4 0.0 26-27 0.0 0.0 0.0 2.401415874799782E-4 0.0 28-29 0.0 0.0 0.0 4.202477780899619E-4 0.0 30-31 0.0 0.0 0.0 0.002221309684189798 0.0 32-33 0.0 0.0 0.0 0.005523256512039498 0.0 34-35 0.0 0.0 0.0 0.009545628102329133 0.0 36-37 0.0 0.0 0.0 0.016149521758028536 0.0 38-39 0.0 0.0 0.0 0.02905713208507736 0.0 40-41 0.0 0.0 0.0 0.04658746797111577 0.0 42-43 0.0 0.0 0.0 0.06399773306341419 0.0 44-45 0.0 0.0 0.0 0.08188828133067257 0.0 46-47 0.0 0.0 0.0 0.1044615905537905 0.0 48-49 0.0 0.0 0.0 0.12547397945828861 0.0 50-51 0.0 0.0 0.0 0.1509289877311663 0.0 52-53 0.0 0.0 0.0 0.17764473933831387 0.0 54-55 0.0 0.0 0.0 0.2083228271388811 0.0 56-57 0.0 0.0 0.0 0.24608509177010765 0.0 58-59 0.0 0.0 0.0 0.2827667192576743 0.0 60-61 0.0 0.0 0.0 0.3339168773909097 0.0 62-63 0.0 0.0 0.0 0.38158498250568534 0.0 64-65 0.0 0.0 0.0 0.43225485746396075 0.0 66-67 0.0 0.0 0.0 0.4885080243311456 0.0 68-69 0.0 0.0 0.0 0.5559878104130195 0.0 70-71 0.0 0.0 0.0 0.6321126936441726 0.0 72-73 0.0 0.0 0.0 0.7107590635438654 0.0 74-75 0.0 0.0 0.0 0.7933677696369779 0.0 76-77 0.0 0.0 0.0 0.8847416436731097 0.0 78-79 0.0 0.0 0.0 0.9922650394672698 0.0 80-81 0.0 0.0 0.0 1.1149773906695388 0.0 82-83 0.0 0.0 0.0 1.2390105206029474 0.0 84-85 0.0 0.0 0.0 1.3615427656146064 0.0 86-87 0.0 0.0 0.0 1.497162727143924 0.0 88-89 0.0 0.0 0.0 1.6596185110741293 0.0 90-91 0.0 0.0 0.0 1.8435669670837926 0.0 92-93 0.0 0.0 0.0 2.039222325483105 0.0 94-95 0.0 0.0 0.0 2.224971843398868 0.0 96-97 0.0 0.0 0.0 2.4158844054454507 0.0 98-99 0.0 0.0 0.0 2.632372046558651 0.0 100-101 0.0 0.0 0.0 2.8811587311879086 0.0 102-103 0.0 0.0 0.0 3.1518583356747136 0.0 104-105 0.0 0.0 0.0 3.4342048071542983 0.0 106-107 0.0 0.0 0.0 3.701422358622644 0.0 108-109 0.0 0.0 0.0 3.9942149891576073 0.0 110-111 0.0 0.0 0.0 4.330413211629577 0.0 112-113 0.0 0.0 0.0 4.691586159199463 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2115 0.0 54.40906 1 GTATCAA 3500 0.0 51.10666 1 ATCAACG 4200 0.0 41.86899 3 TCAACGC 4305 0.0 40.847797 4 CAACGCA 4360 0.0 40.279205 5 TATCAAC 4445 0.0 39.69536 2 AACGCAG 4475 0.0 39.22289 6 ACGCAGA 5155 0.0 34.044876 7 CGCAGAG 5235 0.0 33.524616 8 GTACATG 3370 0.0 31.493027 1 TACATGG 3410 0.0 30.591686 2 GCAGAGT 5750 0.0 30.521975 9 GTGGTAT 1115 0.0 30.480654 1 ACATGGG 3460 0.0 29.288195 3 CCTATAC 250 2.1827873E-11 28.612844 3 GAGTACT 3785 0.0 27.5062 12-13 CATGGGG 2060 0.0 26.911352 4 TGGTATC 1175 0.0 26.887955 2 GTACTTT 3885 0.0 26.491926 14-15 AGAGTAC 5350 0.0 25.353596 10-11 >>END_MODULE