FastQCFastQC Report
Fri 27 May 2016
SRR940085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634902
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA6710.10568560187241495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.052.1565361
GTATCAA12750.049.1305271
TCAACGC14450.043.7253274
CAACGCA14600.043.252195
ATCAACG14950.041.8609283
AACGCAG15350.041.138896
CCGTAAG905.0585277E-639.7723351
TATCAAC16300.038.7963262
TCTACGC700.002751917334.0905762
GTACATG35150.033.2661931
TACATGG34650.032.885352
ACGCAGA19250.032.4947857
ACATGGG36450.030.5777023
CGCAGAG20750.029.8586628
TACCGTG850.007164528.0347167
CATGGGG19200.027.3197364
GCAGAGT24000.025.0627219
GTATAAG4050.025.0418421
TAAGATA3002.1827873E-1023.8426784
GAGTACA26100.023.5433941
Produced by FastQC (version 0.11.5)