##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940085_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634902 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3694459932399 33.0 31.0 34.0 28.0 34.0 2 31.366251799490314 34.0 31.0 34.0 28.0 34.0 3 31.555840113907344 34.0 31.0 34.0 28.0 34.0 4 34.74639078156944 37.0 35.0 37.0 32.0 37.0 5 34.85411291821415 37.0 35.0 37.0 32.0 37.0 6 34.90361977124029 37.0 37.0 37.0 32.0 37.0 7 34.824606002186165 37.0 37.0 37.0 32.0 37.0 8 34.77513852531572 37.0 36.0 37.0 32.0 37.0 9 36.35781427684903 39.0 38.0 39.0 32.0 39.0 10-11 36.39349143647366 39.0 38.5 39.0 32.0 39.0 12-13 36.35389713688097 39.0 38.5 39.0 32.0 39.0 14-15 37.82858063134153 41.0 39.0 41.0 32.0 41.0 16-17 37.87902463687309 41.0 39.0 41.0 32.0 41.0 18-19 37.901869894881415 41.0 39.0 41.0 32.0 41.0 20-21 37.953334530368465 41.0 39.0 41.0 32.0 41.0 22-23 37.98082538722511 41.0 39.0 41.0 32.0 41.0 24-25 38.05776560792059 41.0 39.0 41.0 32.0 41.0 26-27 38.11890102724515 41.0 39.0 41.0 32.5 41.0 28-29 38.08441145247613 41.0 39.0 41.0 32.0 41.0 30-31 38.02280273176018 41.0 39.0 41.0 32.0 41.0 32-33 37.99089623280443 41.0 39.0 41.0 32.0 41.0 34-35 37.9084528005897 41.0 39.0 41.0 32.0 41.0 36-37 37.75353991639655 41.0 38.0 41.0 31.0 41.0 38-39 37.646927399819184 41.0 38.0 41.0 31.0 41.0 40-41 37.551145688625965 41.0 38.0 41.0 31.0 41.0 42-43 37.362974128290666 40.0 38.0 41.0 30.0 41.0 44-45 37.17987500433138 40.0 38.0 41.0 30.0 41.0 46-47 37.00845248558045 40.0 37.0 41.0 30.0 41.0 48-49 36.84366721163266 40.0 37.0 41.0 30.0 41.0 50-51 36.65700690815275 40.0 36.5 41.0 30.0 41.0 52-53 36.782254584172044 40.0 36.5 41.0 30.0 41.0 54-55 36.574527249874784 40.0 36.0 41.0 30.0 41.0 56-57 36.44334952480855 40.0 35.5 41.0 30.0 41.0 58-59 36.23420701147579 39.0 35.0 41.0 30.0 41.0 60-61 35.970196975281226 39.0 35.0 41.0 30.0 41.0 62-63 35.670806675675934 38.5 35.0 41.0 30.0 41.0 64-65 35.3455060781034 37.5 35.0 40.5 29.5 41.0 66-67 35.01236805050228 37.0 35.0 40.0 29.0 41.0 68-69 34.657936972950154 36.5 35.0 39.5 29.0 41.0 70-71 34.28837757638186 36.0 34.5 39.0 28.5 41.0 72-73 33.9234062894746 35.5 34.0 39.0 28.0 41.0 74-75 33.559634400269644 35.0 34.0 37.5 28.0 40.0 76-77 33.21314864341268 35.0 34.0 37.0 27.5 39.0 78-79 32.88805122680351 35.0 34.0 36.5 27.0 39.0 80-81 32.5916157139212 35.0 34.0 36.0 27.0 38.0 82-83 32.308152281769466 35.0 34.0 36.0 26.0 37.0 84-85 32.0910164088316 35.0 33.0 35.0 26.0 37.0 86-87 31.896125701289336 35.0 33.0 35.0 25.5 36.5 88-89 31.736302610481616 35.0 33.0 35.0 25.0 36.0 90-91 31.602528421709174 35.0 33.0 35.0 25.0 36.0 92-93 31.46516470258402 35.0 33.0 35.0 25.0 36.0 94-95 31.35536586748821 35.0 33.0 35.0 24.0 35.0 96-97 31.27354379101027 35.0 33.0 35.0 24.0 35.0 98-99 31.20603573464881 35.0 33.0 35.0 24.0 35.0 100-101 31.10349156247736 35.0 33.0 35.0 23.5 35.0 102-103 30.3858485246542 34.0 31.0 34.5 23.0 35.0 104-105 31.32838847570176 35.0 33.0 35.0 25.0 35.0 106-107 31.453513298115297 35.0 34.0 35.0 25.0 35.0 108-109 31.44540889775115 35.0 34.0 35.0 25.0 35.0 110-111 31.39570673899279 35.0 34.0 35.0 24.5 35.0 112-113 31.346143971825573 35.0 34.0 35.0 24.0 35.0 114-115 31.282783012181408 35.0 33.0 35.0 24.0 35.0 116-117 31.205925481412876 35.0 33.0 35.0 24.0 35.0 118-119 31.12192669104838 35.0 33.0 35.0 23.0 35.0 120-121 31.0403936355532 35.0 33.0 35.0 22.5 35.0 122-123 30.909311988306854 35.0 33.0 35.0 19.5 35.0 124-125 29.629677493534437 34.0 30.5 35.0 8.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0018000812702041458 1101 2 0.09840483457267624 1101 3 0.10934895042825232 1101 4 -0.04226985649102488 1101 5 0.14964649514851658 1101 6 0.28259368145634767 1101 7 0.3919601885768742 1101 8 0.28618995763721244 1101 9 0.3790768507666442 1101 10-11 0.18429381347009155 1101 12-13 0.2769605814586171 1101 14-15 0.34663313238524296 1101 16-17 0.36807066172010394 1101 18-19 0.2912232734326494 1101 20-21 0.24917993895568458 1101 22-23 0.10450354586816246 1101 24-25 0.1430650754260725 1101 26-27 0.260072226547031 1101 28-29 0.3106158649324655 1101 30-31 0.2429032042466801 1101 32-33 0.36908682797351133 1101 34-35 0.21634769912235186 1101 36-37 0.2789138830826303 1101 38-39 0.3632448600623519 1101 40-41 0.2167679560704343 1101 42-43 0.1679936594759326 1101 44-45 0.29242503426844735 1101 46-47 0.3248505145475562 1101 48-49 0.40274575824675907 1101 50-51 0.29079616945873 1101 52-53 0.38137930537406817 1101 54-55 0.3577076509291359 1101 56-57 0.47710155286780065 1101 58-59 0.4439525161710236 1101 60-61 0.37519164405824057 1101 62-63 0.36116202945859754 1101 64-65 0.3405129937082023 1101 66-67 0.46253198394047956 1101 68-69 0.47559366782109436 1101 70-71 0.4431578788584929 1101 72-73 0.44472575568118344 1101 74-75 0.46381868098759327 1101 76-77 0.36490740672444133 1101 78-79 0.403771218291169 1101 80-81 0.21916858717973042 1101 82-83 0.17495601406967154 1101 84-85 0.09430712517031026 1101 86-87 0.153625116016741 1101 88-89 0.298106863289199 1101 90-91 0.21260164879072008 1101 92-93 0.22371386449468034 1101 94-95 0.32520753866078067 1101 96-97 0.4053066111216843 1101 98-99 0.49515157668203 1101 100-101 0.19808562135500907 1101 102-103 0.2125961111922301 1101 104-105 0.34818999124371075 1101 106-107 0.3133317810459886 1101 108-109 0.38652072569261264 1101 110-111 0.43799004472136716 1101 112-113 0.37926289896730125 1101 114-115 0.22157170544977234 1101 116-117 0.21156214769859005 1101 118-119 0.181663046233961 1101 120-121 0.29223293216609036 1101 122-123 0.27979602706652784 1101 124-125 0.16929832372398934 1102 1 -0.23516590784707603 1102 2 -0.0979558389743076 1102 3 0.11024472598882795 1102 4 -0.012976089754793918 1102 5 0.017548188545504217 1102 6 0.12719091598754062 1102 7 -0.02901620678414929 1102 8 0.038317424593572014 1102 9 0.20230888522541335 1102 10-11 0.020278737757998044 1102 12-13 0.09530396844319 1102 14-15 0.24783099395933306 1102 16-17 0.2694138485846054 1102 18-19 0.1968714242278935 1102 20-21 -0.056532059672065316 1102 22-23 -0.11626701170447973 1102 24-25 -0.001647942861090712 1102 26-27 0.11481840758899153 1102 28-29 0.1591607061228899 1102 30-31 0.31437465854782687 1102 32-33 0.2965170956579257 1102 34-35 0.2677833224010868 1102 36-37 0.09166343757723183 1102 38-39 0.06478721404617005 1102 40-41 0.136026654146427 1102 42-43 0.1757554594130326 1102 44-45 0.12634681590886032 1102 46-47 0.022881237743376914 1102 48-49 0.047213391207485245 1102 50-51 -0.011859233306736883 1102 52-53 0.10966389517557218 1102 54-55 0.24595726736566803 1102 56-57 0.1547438525333149 1102 58-59 0.2583316198236929 1102 60-61 0.23410222850414897 1102 62-63 0.21919113263479062 1102 64-65 0.34455113087621214 1102 66-67 0.2517960185517296 1102 68-69 0.1418386529073814 1102 70-71 0.052323378158078526 1102 72-73 0.021826778199461216 1102 74-75 0.1523246417068833 1102 76-77 0.006513799131312226 1102 78-79 0.09334925082497847 1102 80-81 0.10164569249052846 1102 82-83 0.11648998206952399 1102 84-85 0.13816200952950197 1102 86-87 0.13776321792551016 1102 88-89 0.08622735463162456 1102 90-91 0.014013448251347427 1102 92-93 0.1739292318103054 1102 94-95 0.14722694077824272 1102 96-97 0.11709558927047681 1102 98-99 0.07911624119502036 1102 100-101 0.17534816936000652 1102 102-103 0.20278069813486965 1102 104-105 0.2758751737818059 1102 106-107 0.2123473808820826 1102 108-109 0.18556205525225167 1102 110-111 0.1793777344780807 1102 112-113 0.10299475712981732 1102 114-115 0.06880385426287106 1102 116-117 0.1738133079185289 1102 118-119 0.1477474587862524 1102 120-121 0.205912092042972 1102 122-123 0.18624227576812658 1102 124-125 0.1676949807658339 1103 1 -0.1835859501801025 1103 2 -0.05435538455922284 1103 3 -0.07088038396968699 1103 4 -0.1083199374456214 1103 5 -0.04273711875350017 1103 6 0.1221372305946744 1103 7 0.02209747870485046 1103 8 -0.0838058282877796 1103 9 0.08334859206679823 1103 10-11 0.01736653953303602 1103 12-13 0.04739330042417578 1103 14-15 0.23753294829669613 1103 16-17 0.18624995952212942 1103 18-19 0.17138828773832415 1103 20-21 -0.00948663899361435 1103 22-23 -0.09634148706815893 1103 24-25 0.04687881331060595 1103 26-27 0.13382741096825868 1103 28-29 0.22366729882693903 1103 30-31 0.12768152172178304 1103 32-33 0.17316006416132979 1103 34-35 0.1794856324512537 1103 36-37 0.28169739052380294 1103 38-39 0.23620713300133644 1103 40-41 0.37136351576613436 1103 42-43 0.25913966608538175 1103 44-45 0.17811824960767808 1103 46-47 0.23336627297015866 1103 48-49 0.36677086526207603 1103 50-51 0.31403457520761435 1103 52-53 0.27881093755185304 1103 54-55 0.36463315051882006 1103 56-57 0.24618687679709694 1103 58-59 0.14837951286629902 1103 60-61 0.12863498692868802 1103 62-63 0.031044700738576125 1103 64-65 0.13673474277112518 1103 66-67 0.005881508193596119 1103 68-69 -0.08141278636747273 1103 70-71 -0.0477929495399394 1103 72-73 -0.16135249303795973 1103 74-75 -0.19651023865664996 1103 76-77 -0.2664885610711565 1103 78-79 -0.29028139110843654 1103 80-81 -0.2123637835701615 1103 82-83 -0.17788959552954253 1103 84-85 -0.15885897592313114 1103 86-87 0.090890846715169 1103 88-89 -0.03469416583227414 1103 90-91 -0.026097976378398613 1103 92-93 -0.07547991641777685 1103 94-95 -0.13974355588975484 1103 96-97 -0.07794798554697735 1103 98-99 -0.05496264693009678 1103 100-101 -0.19067388803794216 1103 102-103 -0.23545986946738395 1103 104-105 -0.010061833007181775 1103 106-107 -0.0756606114396483 1103 108-109 0.07904850531866714 1103 110-111 0.0022103299477080895 1103 112-113 -0.02709037359463551 1103 114-115 -0.21208988214753788 1103 116-117 -0.21841212426450696 1103 118-119 -0.16709601957214915 1103 120-121 -0.20176201094389512 1103 122-123 -0.2861819710260498 1103 124-125 -0.09539549045748075 1104 1 0.24515289399508333 1104 2 0.285875367340239 1104 3 0.2883825492768608 1104 4 0.30328462376903076 1104 5 0.18337251812675248 1104 6 0.2868741846946392 1104 7 0.3683770971850251 1104 8 0.28683823675083175 1104 9 0.54088917861597 1104 10-11 0.4532815675086468 1104 12-13 0.4269565491004954 1104 14-15 0.437948983895204 1104 16-17 0.4053234471490299 1104 18-19 0.2946523287441991 1104 20-21 0.4020357790509692 1104 22-23 0.5575948136415576 1104 24-25 0.49761481611442804 1104 26-27 0.38473567973445455 1104 28-29 0.38129534829433 1104 30-31 0.4388727072120133 1104 32-33 0.5539844060771841 1104 34-35 0.5564429868466192 1104 36-37 0.49266608537053 1104 38-39 0.40530080437858373 1104 40-41 0.37264651385103065 1104 42-43 0.3887389012933511 1104 44-45 0.5221221111189891 1104 46-47 0.43802950161919796 1104 48-49 0.45075873623628837 1104 50-51 0.4026568856055306 1104 52-53 0.39593301638426226 1104 54-55 0.46073440097256224 1104 56-57 0.35820747361024985 1104 58-59 0.48390698154236844 1104 60-61 0.5626497695700365 1104 62-63 0.5195297929266758 1104 64-65 0.4557686452131975 1104 66-67 0.43691410260347396 1104 68-69 0.4251023404928702 1104 70-71 0.493444486466629 1104 72-73 0.4395240137024743 1104 74-75 0.4282284678309338 1104 76-77 0.41464964598596055 1104 78-79 0.6031976928935165 1104 80-81 0.6268632570226558 1104 82-83 0.715975952529682 1104 84-85 0.46462958974759516 1104 86-87 0.5892206022571003 1104 88-89 0.7122610606638275 1104 90-91 0.6208608487708673 1104 92-93 0.6043932858202652 1104 94-95 0.4647519442291568 1104 96-97 0.6258766004514449 1104 98-99 0.5830942055313777 1104 100-101 0.5683274387698347 1104 102-103 0.3340817559973104 1104 104-105 0.4390266124998199 1104 106-107 0.48711711070677666 1104 108-109 0.35710157137116383 1104 110-111 0.38689696548863495 1104 112-113 0.3467760506584092 1104 114-115 0.3175340742430457 1104 116-117 0.39395190923113077 1104 118-119 0.3424983026919364 1104 120-121 0.4677103013315822 1104 122-123 0.3777249458982581 1104 124-125 0.3827403446178117 1105 1 -0.20195931722603788 1105 2 -0.0036730875052448653 1105 3 -0.12837262624069368 1105 4 -0.1614678514784913 1105 5 -0.4495332724523067 1105 6 -0.24972347507133463 1105 7 -0.3117354140660993 1105 8 -0.26809575664850627 1105 9 -0.09194160446233468 1105 10-11 -0.1990714177898809 1105 12-13 -0.04488038459287935 1105 14-15 0.06828726772358351 1105 16-17 -0.027055790774767274 1105 18-19 -0.31322420890957403 1105 20-21 -0.42461363589051615 1105 22-23 -0.2681462604658549 1105 24-25 -0.30961890725790653 1105 26-27 -0.20984802863637952 1105 28-29 -0.16393642488298354 1105 30-31 -0.2736700470634119 1105 32-33 -0.2420979521586375 1105 34-35 -0.34940759821189005 1105 36-37 -0.14081726296430475 1105 38-39 -0.21136211191304 1105 40-41 -0.12672942374273788 1105 42-43 -0.20760898349512757 1105 44-45 -0.32562691378350195 1105 46-47 -0.21362066339730745 1105 48-49 -2.2986262360547016E-4 1105 50-51 -0.07540342042506865 1105 52-53 0.002773700452671335 1105 54-55 0.1462084483773083 1105 56-57 0.06985388825171412 1105 58-59 0.026212712115423642 1105 60-61 0.08302030662374449 1105 62-63 -0.029086318684484525 1105 64-65 -0.11228491014233555 1105 66-67 -0.16799888869564938 1105 68-69 -0.1862267924204204 1105 70-71 -0.13186604458648787 1105 72-73 -0.17224166286518283 1105 74-75 -0.14914326934277966 1105 76-77 -0.10622269124776551 1105 78-79 -0.19408478535430618 1105 80-81 -0.3097081501180625 1105 82-83 -0.25426932199776786 1105 84-85 -0.1581831915305294 1105 86-87 -0.25844141394452436 1105 88-89 -0.15762792823506544 1105 90-91 -0.3358125685708657 1105 92-93 -0.2413282863369446 1105 94-95 -0.21142267323259034 1105 96-97 -0.3180927964882514 1105 98-99 -0.13484158804797985 1105 100-101 -0.17243613758780185 1105 102-103 -0.28772742492077796 1105 104-105 -0.23463525368679683 1105 106-107 -0.12481358236253115 1105 108-109 -0.2084257313591067 1105 110-111 -0.21275499970788303 1105 112-113 -0.15355323226988205 1105 114-115 -0.3213355627206518 1105 116-117 -0.23467845380517005 1105 118-119 -0.21193914105244005 1105 120-121 -0.16737745744429944 1105 122-123 -0.22997157375370492 1105 124-125 -0.09216214563121383 1106 1 0.35962260367791643 1106 2 0.44263405168324965 1106 3 0.3977818972019662 1106 4 0.23494861341254847 1106 5 0.32604087091844747 1106 6 0.5812428467424411 1106 7 0.40678538716656476 1106 8 0.37900674536925294 1106 9 0.4961279530987426 1106 10-11 0.5513130564929014 1106 12-13 0.4783538952515727 1106 14-15 0.5696270578769358 1106 16-17 0.6044879761927291 1106 18-19 0.5595742355837956 1106 20-21 0.511796225434594 1106 22-23 0.5215419044765497 1106 24-25 0.5296671738925838 1106 26-27 0.6237993409432008 1106 28-29 0.7532372489008594 1106 30-31 0.7345029663638414 1106 32-33 0.6692227610264823 1106 34-35 0.6390251554751032 1106 36-37 0.7543291160803776 1106 38-39 0.6513167346641069 1106 40-41 0.6488014295847293 1106 42-43 0.6493665833109432 1106 44-45 0.5601812893128368 1106 46-47 0.5584535900328831 1106 48-49 0.6337682296795748 1106 50-51 0.6019023246772903 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2216 120-121 -1.1515510694458335 2216 122-123 -1.125459884220863 2216 124-125 -1.1370546532176569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12105.0 3 2037.0 4 1270.0 5 983.0 6 1004.0 7 1116.0 8 1235.0 9 1360.0 10 1536.0 11 1849.0 12 2141.0 13 2241.0 14 2200.0 15 2220.0 16 1922.0 17 1926.0 18 1436.0 19 1410.0 20 1297.0 21 1268.0 22 1370.0 23 1848.0 24 3336.0 25 5785.0 26 6706.0 27 5264.0 28 5439.0 29 6547.0 30 7092.0 31 8483.0 32 10764.0 33 15088.0 34 23075.0 35 43054.0 36 108535.0 37 233488.0 38 106041.0 39 431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51901742265702 18.191359269811656 13.202201154786819 24.08742215274451 2 15.310079048576448 19.546729244817023 40.44654791757738 24.69664378902915 3 19.732209109427508 24.510790879544516 27.439665127475582 28.317334883552398 4 12.912452656442666 16.172180752261927 35.601566713024724 35.313799878270686 5 14.341571922108079 38.203652621314255 32.41295762133788 15.041817835239776 6 32.87220566084112 35.75698466619188 17.036094007020903 14.334715665946089 7 29.950257535817883 32.46716004720571 19.170937921971927 18.411644495004484 8 26.944335838966804 36.13582761578045 18.571379023307436 18.348457521945313 9 26.80836167706594 17.87492174922222 18.580716170040322 36.73600040367152 10-11 25.681264334580028 27.496866188000357 25.50509328162708 21.316776195792535 12-13 25.977151482112866 24.332442626618477 26.36386839808288 23.32653749318578 14-15 22.958113554397404 25.66059700058363 23.921767231364306 27.459522213654665 16-17 23.519462810210324 29.02693432675212 24.45721317041391 22.996389692623644 18-19 23.628394099677656 27.651871211542208 25.886911801668987 22.83282288711115 20-21 24.368801735218256 26.74060333393562 25.8366202695143 23.05397466133182 22-23 24.09244566656996 26.55692238802205 25.83344895874065 23.51718298666734 24-25 23.766073280604253 26.89206825568456 25.802600096463323 23.539258367247864 26-27 23.293788879559806 27.300323087271373 25.69465338611528 23.71123464705354 28-29 23.3611634626971 27.257670349610585 25.85563668410611 23.525529503586203 30-31 23.504635321377293 26.769876829851295 25.869275352502584 23.856212496268824 32-33 23.241002313066502 27.023342518919186 25.796637587291286 23.93901758072303 34-35 23.783754834776843 27.208481956860915 25.51147626972207 23.496286938640175 36-37 24.107803134295562 26.773663348714205 25.475779262048942 23.64275425494129 38-39 23.533544247470488 26.86761003094405 25.684033610798046 23.914812110787416 40-41 23.698264299895964 26.524260344777346 25.986200400552235 23.791274954774455 42-43 23.621926314239154 26.554849258361696 25.669829383415937 24.15339504398321 44-45 23.46240632592867 26.83447599620381 25.87456042592906 23.828557251938456 46-47 23.83004028843844 27.12156238800562 25.38856879556079 23.659828527995145 48-49 23.774207294068166 26.85051358412485 25.540501903344893 23.83477721846209 50-51 23.662261357192364 26.99026526368849 25.644414340621257 23.70305903849789 52-53 24.006745224429938 26.594094856884514 25.787645149526757 23.61151476915879 54-55 23.645081968504307 26.683280378439772 25.76852856807552 23.903109084980397 56-57 23.67881728391172 27.138041303697168 25.64168806414518 23.541453348245934 58-59 23.710283782070114 26.510927448859917 25.723829770140384 24.054958998929585 60-61 23.48951372245723 26.679383511314025 26.110118318635717 23.720984447593025 62-63 23.40333023519589 26.951196458671372 26.010885489689507 23.634587816443233 64-65 23.24592119121462 26.83699023260101 25.876005473335606 24.041083102848766 66-67 23.333401603186207 27.095831967936274 25.65901414131229 23.911752287565225 68-69 23.376745124051318 26.643815868044125 26.204472370280623 23.774966637623937 70-71 23.468289777990638 26.658138347816774 25.99874280816954 23.87482906602305 72-73 23.39732078158573 26.836291689508073 25.797558962338456 23.96882856656774 74-75 23.327145524812533 27.15883503281481 25.718417170656714 23.79560227171594 76-77 23.622370341317115 26.88558469369512 25.628593863584992 23.863451101402774 78-79 23.68281340092822 26.951203758259002 25.760127417835342 23.60585542297743 80-81 23.65568021777555 27.170587697823823 25.75482606627157 23.41890601812906 82-83 23.690613566277683 27.04969103103868 25.763482192439024 23.496213210244616 84-85 23.717357983500392 26.950880824444752 25.65197391946793 23.67978727258693 86-87 23.61687127558596 27.052962004576624 25.72952236913103 23.600644350706386 88-89 23.575726257115264 27.148207024437276 25.64275664970925 23.633310068738215 90-91 23.62430434388748 27.29193334304856 25.707196225240942 23.376566087823015 92-93 23.546895432559754 26.81539600762553 25.959808416442154 23.67790014337256 94-95 23.468415173824066 26.8796740769215 26.06053397316428 23.591376776090154 96-97 23.674645837009404 26.757423558770892 25.896829548032567 23.671101056187137 98-99 23.411018451265875 26.870847441070488 25.800261226187533 23.9178728814761 100-101 23.170407992066536 26.935888761373043 26.16341580028971 23.73028744627071 102-103 23.279087042727717 26.40459661099194 26.196003488105546 24.120312858174795 104-105 23.17350788887051 26.846263518995517 26.01452528928484 23.965703302849132 106-107 23.292671879000334 26.93369045040077 25.814128443980543 23.95950922661835 108-109 23.482201371209637 26.81207937388148 25.707738890429262 23.99798036447962 110-111 23.24107353136936 27.118222197016777 25.97980256260599 23.660901709007874 112-113 23.16823790378483 27.064060471623208 26.08870662818396 23.678994996408 114-115 22.988625247508377 27.170236930691356 26.011338522148552 23.829799299651715 116-117 23.04901753844312 27.120633110387914 26.12738133118264 23.70296801998633 118-119 23.0906965422603 27.3459520009579 25.722719115958036 23.840632340823763 120-121 22.972298104159382 27.558140267629554 25.959972146865418 23.509589481345646 122-123 22.98542754454795 27.35015402646688 25.972461910841872 23.6919565181433 124-125 23.138518250129962 27.469084264087336 25.756628176247265 23.635769309535437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 445.0 1 445.0 2 446.5 3 408.0 4 319.0 5 249.0 6 210.5 7 198.5 8 217.0 9 204.0 10 180.0 11 174.5 12 170.0 13 189.0 14 205.5 15 216.5 16 236.0 17 236.0 18 281.0 19 385.5 20 471.5 21 618.0 22 812.5 23 1109.5 24 1534.5 25 1976.5 26 2540.5 27 3176.0 28 3867.0 29 4688.0 30 5598.0 31 6674.0 32 8041.5 33 8994.5 34 9647.5 35 10389.0 36 11616.0 37 13156.0 38 14120.0 39 14592.0 40 14992.5 41 16338.5 42 17596.5 43 18410.0 44 19728.0 45 21343.0 46 22099.5 47 22590.0 48 23393.0 49 23622.5 50 23801.5 51 23828.5 52 24463.0 53 25966.0 54 26730.5 55 25808.0 56 23377.0 57 21276.5 58 20139.5 59 18179.0 60 15450.0 61 12827.5 62 10454.5 63 8106.0 64 6315.5 65 4973.5 66 3562.5 67 2822.5 68 2203.5 69 1425.0 70 981.5 71 731.5 72 601.0 73 516.5 74 377.5 75 296.0 76 208.0 77 132.0 78 92.5 79 63.0 80 53.0 81 37.0 82 26.0 83 20.0 84 16.5 85 19.0 86 16.0 87 8.5 88 7.5 89 6.5 90 7.0 91 8.0 92 5.5 93 3.0 94 4.5 95 5.0 96 3.5 97 4.5 98 2.5 99 1.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05134650701998103 2 0.19546323684600142 3 0.024098207282383738 4 0.11151327291456005 5 0.019688077845084754 6 0.03559604474391324 7 0.036698577103237984 8 0.09418776441088546 9 0.11387584225597022 10-11 0.04591259753473764 12-13 0.03150092455213561 14-15 0.012364112886713225 16-17 0.030162135258669842 18-19 0.05142525933136137 20-21 0.19002932736075803 22-23 0.10552809724965427 24-25 0.07308214496095461 26-27 0.014017911425700346 28-29 0.006063927976286103 30-31 0.007638974203892884 32-33 0.00480389099420068 34-35 0.007638974203892884 36-37 0.0035438540121152558 38-39 0.007323964958371528 40-41 0.0044888817486793235 42-43 0.009056515808738987 44-45 0.008584001940456953 46-47 0.016459233078490853 48-49 0.015514205341926785 50-51 0.009765286611162037 52-53 0.012994131377755937 54-55 0.013387892934657633 56-57 0.01449042529398238 58-59 0.01598671921020882 60-61 0.007638974203892884 62-63 0.01764051774919594 64-65 0.029689621390387805 66-67 0.026145767378272552 68-69 0.032288447665938996 70-71 0.024255711905144414 72-73 0.014175416048461023 74-75 0.008111488072174919 76-77 0.008268992694935596 78-79 0.022050647186494923 80-81 0.018900554731281362 82-83 0.020396848647507803 84-85 0.015592957653307125 86-87 0.024334464216524753 88-89 0.027563308983118654 90-91 0.02512198733032815 92-93 0.03024088757005018 94-95 0.023783198036862383 96-97 0.026145767378272552 98-99 0.03197343842041764 100-101 0.01929431628818306 102-103 0.02732705204897764 104-105 0.02315317954581967 106-107 0.029847126013148484 108-109 0.021105619449930856 110-111 0.018664297797140347 112-113 0.02488573039618713 114-115 0.011812846707050852 116-117 0.010710314347726105 118-119 0.028508336719682725 120-121 0.022680665677537636 122-123 0.017089251569533566 124-125 0.01764051774919594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 634902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.450626732657824 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0488710618947 48.57602669262487 2 11.92066574443474 14.650647621320791 3 3.8586829595160093 7.11355458674657 4 1.7025175782124349 4.184830888180835 5 0.9511843668300787 2.9225437740007316 6 0.5753939095455617 2.121498981583739 7 0.3784746772627356 1.6280254284164848 8 0.27522418972310886 1.3530159160378368 9 0.20687436122018002 1.1441303236708473 >10 1.0054688137369943 10.867343774519773 >50 0.05203480974634861 2.1593835616292214 >100 0.023325885800278428 2.802041766329329 >500 0.00128164207691999 0.4769566849389978 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 671 0.10568560187241495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.875231138033901E-5 14-15 0.0 0.0 0.0 0.0 2.3625693414101704E-4 16-17 0.0 0.0 0.0 0.0 4.725138682820341E-4 18-19 0.0 0.0 0.0 0.0 4.725138682820341E-4 20-21 0.0 0.0 0.0 0.0 4.725138682820341E-4 22-23 0.0 0.0 0.0 0.0 4.725138682820341E-4 24-25 0.0 0.0 0.0 1.5750462276067803E-4 4.725138682820341E-4 26-27 0.0 0.0 0.0 2.3625693414101704E-4 6.300184910427121E-4 28-29 0.0 0.0 0.0 0.0011025323593247462 6.300184910427121E-4 30-31 0.0 0.0 0.0 0.0025200739641708485 6.300184910427121E-4 32-33 0.0 0.0 0.0 0.005985175664905765 6.300184910427121E-4 34-35 0.0 0.0 0.0 0.010710314347726107 6.300184910427121E-4 36-37 0.0 0.0 0.0 0.02000308709060611 6.300184910427121E-4 38-39 0.0 0.0 0.0 0.03205219073179798 6.300184910427121E-4 40-41 0.0 0.0 0.0 0.04866892843304951 6.300184910427121E-4 42-43 0.0 0.0 0.0 0.06583693231396341 7.875231138033901E-4 44-45 0.0 0.0 0.0 0.08702130407527461 8.662754251837292E-4 46-47 0.0 0.0 0.0 0.11237954833974377 9.450277365640682E-4 48-49 0.0 0.0 0.0 0.13844656340663597 9.450277365640682E-4 50-51 0.0 0.0 0.0 0.16908121253358788 0.0011025323593247462 52-53 0.0 0.0 0.0 0.2000308709060611 0.0011025323593247462 54-55 0.0 0.0 0.0 0.23302808937442315 0.0011025323593247462 56-57 0.0 0.0 0.0 0.2706716942142252 0.0011025323593247462 58-59 0.0 0.0 0.0 0.3178443287310483 0.0011025323593247462 60-61 0.0 0.0 0.0 0.3710021389127771 0.0011025323593247462 62-63 0.0 0.0 0.0 0.42463246296278795 0.0011025323593247462 64-65 0.0 0.0 0.0 0.47731775927623477 0.0011025323593247462 66-67 0.0 0.0 0.0 0.5400045991349847 0.0011025323593247462 68-69 0.0 0.0 0.0 0.6134017533414606 0.0011025323593247462 70-71 0.0 0.0 0.0 0.6954616617997738 0.0011025323593247462 72-73 0.0 0.0 0.0 0.7787028549287922 0.0011025323593247462 74-75 0.0 0.0 0.0 0.8665904344292505 0.0011025323593247462 76-77 0.0 0.0 0.0 0.9690471915350716 0.0011025323593247462 78-79 0.0 0.0 0.0 1.08016670289273 0.0011025323593247462 80-81 0.0 0.0 0.0 1.2107380351613322 0.0011025323593247462 82-83 0.0 0.0 0.0 1.3475308000289807 0.0012600369820854242 84-85 0.0 0.0 0.0 1.4821972524893605 0.0012600369820854242 86-87 0.0 0.0 0.0 1.6227701283032658 0.0012600369820854242 88-89 0.0 0.0 0.0 1.785708660549187 0.0012600369820854242 90-91 0.0 0.0 0.0 1.9894408900901241 0.0012600369820854242 92-93 0.0 0.0 0.0 2.194748165858668 0.0012600369820854242 94-95 0.0 0.0 0.0 2.3980866338427034 0.0012600369820854242 96-97 0.0 0.0 0.0 2.602921395742965 0.0012600369820854242 98-99 0.0 0.0 0.0 2.8346894481353027 0.0012600369820854242 100-101 0.0 0.0 0.0 3.10811747324784 0.0012600369820854242 102-103 0.0 0.0 0.0 3.404619925594816 0.0012600369820854242 104-105 0.0 0.0 0.0 3.6960822300134506 0.0012600369820854242 106-107 0.0 0.0 0.0 3.9845519465996326 0.0012600369820854242 108-109 0.0 0.0 0.0 4.293497264144703 0.0014175416048461023 110-111 0.0 0.0 0.0 4.657254190410488 0.0014175416048461023 112-113 0.0 0.0 0.0 5.036210312772679 0.0014175416048461023 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 835 0.0 52.156536 1 GTATCAA 1275 0.0 49.130527 1 TCAACGC 1445 0.0 43.725327 4 CAACGCA 1460 0.0 43.25219 5 ATCAACG 1495 0.0 41.860928 3 AACGCAG 1535 0.0 41.13889 6 CCGTAAG 90 5.0585277E-6 39.772335 1 TATCAAC 1630 0.0 38.796326 2 TCTACGC 70 0.0027519173 34.090576 2 GTACATG 3515 0.0 33.266193 1 TACATGG 3465 0.0 32.88535 2 ACGCAGA 1925 0.0 32.494785 7 ACATGGG 3645 0.0 30.577702 3 CGCAGAG 2075 0.0 29.858662 8 TACCGTG 85 0.0071645 28.034716 7 CATGGGG 1920 0.0 27.319736 4 GCAGAGT 2400 0.0 25.062721 9 GTATAAG 405 0.0 25.041842 1 TAAGATA 300 2.1827873E-10 23.842678 4 GAGTACA 2610 0.0 23.543394 1 >>END_MODULE