FastQCFastQC Report
Fri 27 May 2016
SRR940085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634902
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.5772544424178849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26830.4225849028668991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.26413525236965707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.12789375368167055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.070.250861
GTATCAA31500.058.266661
TATCAAC39050.046.837592
ATCAACG39400.046.7239463
TCAACGC40050.045.816874
CAACGCA40750.045.1297725
AACGCAG41400.044.4072156
GTGGTAT6550.042.7598841
ACGCAGA48150.038.0583427
CGCAGAG49100.037.321978
ATAACGC851.6967056E-435.045183
TGGTATC7700.034.8176162
GCAGAGT53600.034.0776029
TAACGCA700.002770471934.0438884
GTACATG35950.032.32331
ACATGGG35800.031.9518073
TACATGG36150.031.807262
GTTCGCA800.005320555529.7954521
CTATTAC1403.0549654E-629.795451
GAGTACT34400.028.4513512-13
Produced by FastQC (version 0.11.5)