##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634902 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.119569634368766 34.0 33.0 34.0 31.0 34.0 2 33.16526172543164 34.0 33.0 34.0 31.0 34.0 3 33.22243117835509 34.0 33.0 34.0 31.0 34.0 4 36.45383066993016 37.0 37.0 37.0 35.0 37.0 5 36.459007846880304 37.0 37.0 37.0 35.0 37.0 6 36.54069446938268 37.0 37.0 37.0 35.0 37.0 7 36.52841068385357 37.0 37.0 37.0 35.0 37.0 8 36.53102998573008 37.0 37.0 37.0 35.0 37.0 9 38.40046967878507 39.0 39.0 39.0 37.0 39.0 10-11 38.37949715074137 39.0 39.0 39.0 37.0 39.0 12-13 38.35758747019225 39.0 39.0 39.0 37.0 39.0 14-15 39.96425826348003 41.0 40.0 41.0 38.0 41.0 16-17 39.97277532595582 41.0 40.0 41.0 38.0 41.0 18-19 40.01019133661573 41.0 40.0 41.0 38.0 41.0 20-21 40.00542839682345 41.0 40.0 41.0 38.0 41.0 22-23 39.96464021219023 41.0 40.0 41.0 38.0 41.0 24-25 39.92633745050417 41.0 40.0 41.0 38.0 41.0 26-27 39.83034704568579 41.0 40.0 41.0 37.0 41.0 28-29 39.746935747564194 41.0 40.0 41.0 37.0 41.0 30-31 39.59240087446567 41.0 40.0 41.0 36.5 41.0 32-33 39.406728912493584 41.0 40.0 41.0 35.0 41.0 34-35 39.294385590217075 41.0 39.5 41.0 35.0 41.0 36-37 39.179971869674375 41.0 39.0 41.0 35.0 41.0 38-39 39.05322081203083 41.0 39.0 41.0 35.0 41.0 40-41 38.902243968360466 41.0 39.0 41.0 35.0 41.0 42-43 38.72559780879568 41.0 39.0 41.0 35.0 41.0 44-45 38.498807690005705 41.0 38.5 41.0 35.0 41.0 46-47 38.17103584490205 41.0 38.0 41.0 34.5 41.0 48-49 37.86457988791971 41.0 38.0 41.0 34.0 41.0 50-51 37.85963739285748 41.0 38.0 41.0 34.0 41.0 52-53 37.73736340411591 41.0 37.5 41.0 34.0 41.0 54-55 37.554519437645496 40.5 37.0 41.0 34.0 41.0 56-57 37.3434073290051 40.0 36.5 41.0 33.0 41.0 58-59 37.09855064246136 40.0 36.0 41.0 33.0 41.0 60-61 36.83427127336188 39.0 35.5 41.0 33.0 41.0 62-63 36.55022743667527 39.0 35.0 41.0 32.5 41.0 64-65 36.22987720939609 38.5 35.0 41.0 32.0 41.0 66-67 35.90135800485744 37.5 35.0 41.0 32.0 41.0 68-69 35.539445457724185 37.0 35.0 40.0 31.5 41.0 70-71 35.1673927629776 36.5 35.0 39.0 31.0 41.0 72-73 34.80879253806099 36.0 35.0 39.0 31.0 41.0 74-75 34.46545923622858 35.5 35.0 38.0 31.0 40.5 76-77 34.125546934802536 35.0 35.0 37.0 31.0 39.0 78-79 33.82150709873335 35.0 35.0 37.0 30.5 39.0 80-81 33.53788143682017 35.0 35.0 36.0 30.0 38.5 82-83 33.29287669593103 35.0 35.0 36.0 30.0 37.0 84-85 33.07867119649961 35.0 35.0 36.0 30.0 37.0 86-87 32.89417106892087 35.0 35.0 35.0 29.0 36.5 88-89 32.81021322975829 35.0 34.5 35.0 29.5 36.0 90-91 32.83023994254231 35.0 35.0 35.0 30.0 36.0 92-93 32.75886436016897 35.0 35.0 35.0 30.0 36.0 94-95 32.696474416524126 35.0 35.0 35.0 30.0 36.0 96-97 32.642633823802726 35.0 35.0 35.0 30.0 35.5 98-99 32.58689136276149 35.0 35.0 35.0 30.0 35.0 100-101 32.531929337126044 35.0 35.0 35.0 30.0 35.0 102-103 32.04803969746512 35.0 33.5 35.0 28.0 35.0 104-105 32.4409727800511 35.0 34.5 35.0 29.0 35.0 106-107 32.49199640259441 35.0 35.0 35.0 30.0 35.0 108-109 32.47030014080913 35.0 35.0 35.0 30.0 35.0 110-111 32.427348630182294 35.0 35.0 35.0 30.0 35.0 112-113 32.4049955741201 35.0 35.0 35.0 29.5 35.0 114-115 32.348641207619444 35.0 35.0 35.0 29.0 35.0 116-117 32.293086177079296 35.0 35.0 35.0 29.0 35.0 118-119 32.23142547983784 35.0 35.0 35.0 29.0 35.0 120-121 32.15347565451046 35.0 35.0 35.0 29.0 35.0 122-123 32.047908181105115 35.0 34.5 35.0 29.0 35.0 124-125 30.981001004879495 34.5 32.5 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1552126707158834 1101 2 0.17903291537169252 1101 3 0.16400812182637736 1101 4 -0.02804112806411041 1101 5 0.019040530142731882 1101 6 0.09014846330672555 1101 7 0.13730239288118895 1101 8 0.15127346100376826 1101 9 0.2113161309988314 1101 10-11 0.16825015421652267 1101 12-13 0.1861694397002509 1101 14-15 0.2043821239626311 1101 16-17 0.19447076747933068 1101 18-19 0.25878991947872976 1101 20-21 0.2951781798505735 1101 22-23 0.3262718390326924 1101 24-25 0.32453824774021456 1101 26-27 0.28441367806426854 1101 28-29 0.28946871253452855 1101 30-31 0.34018011848296226 1101 32-33 0.2741574861688889 1101 34-35 0.3302122352582373 1101 36-37 0.38548518916891084 1101 38-39 0.39673678662525447 1101 40-41 0.3244785696853185 1101 42-43 0.17979788610299607 1101 44-45 0.3521808303027072 1101 46-47 0.3139976853140638 1101 48-49 0.31520905918431197 1101 50-51 0.3214644709230683 1101 52-53 0.23895187755794467 1101 54-55 0.15733413300478816 1101 56-57 0.09381336496699788 1101 58-59 0.1251271694109306 1101 60-61 0.19125265288095505 1101 62-63 0.2221159512151729 1101 64-65 0.2360297890664711 1101 66-67 0.3335428839399839 1101 68-69 0.29438214953096065 1101 70-71 0.21591926948374152 1101 72-73 0.18842834203095293 1101 74-75 0.20429971517018686 1101 76-77 0.08294688292723151 1101 78-79 0.06472194782482177 1101 80-81 -0.04176313073568849 1101 82-83 0.05900194814657311 1101 84-85 0.08304610030636184 1101 86-87 0.12907330042312282 1101 88-89 0.04614997655887265 1101 90-91 0.1890437986178739 1101 92-93 0.10504848129635036 1101 94-95 0.19307501232901814 1101 96-97 0.15992669457070718 1101 98-99 0.23385355425784127 1101 100-101 0.32768008217895783 1101 102-103 0.261957682870424 1101 104-105 0.27901952996533197 1101 106-107 0.2820900002316975 1101 108-109 0.1506221759040116 1101 110-111 0.20150944311887287 1101 112-113 0.27424667278708625 1101 114-115 0.2198620912452398 1101 116-117 0.1090584561806267 1101 118-119 0.055855652164474634 1101 120-121 0.11271412392589752 1101 122-123 0.22107176787660165 1101 124-125 0.08442778948874974 1102 1 0.18139981157182206 1102 2 0.20342850510648702 1102 3 0.2307862839617556 1102 4 -0.00785758936946479 1102 5 0.11085752510161484 1102 6 0.11521111994832722 1102 7 0.19195899636708447 1102 8 0.14405484411935277 1102 9 0.18934104118304163 1102 10-11 0.17535203439225455 1102 12-13 0.2059098553782306 1102 14-15 0.26989762373868587 1102 16-17 0.27535262993013276 1102 18-19 0.2534414723151954 1102 20-21 0.2881573006563869 1102 22-23 0.34452803084302275 1102 24-25 0.2605307956499203 1102 26-27 0.19471700291632033 1102 28-29 0.21350408613965044 1102 30-31 0.20594481539635012 1102 32-33 0.22709353351158512 1102 34-35 0.208274787075041 1102 36-37 0.2628759093152766 1102 38-39 0.17499898203773512 1102 40-41 0.1420572121088668 1102 42-43 0.2523580888913344 1102 44-45 0.16683152548171876 1102 46-47 0.12075078479280421 1102 48-49 0.20410430109136257 1102 50-51 0.12805556843872523 1102 52-53 0.21111136372583417 1102 54-55 0.16068376182970212 1102 56-57 0.08263284713248709 1102 58-59 0.23442131732825544 1102 60-61 0.22991870965787342 1102 62-63 0.24198025074727525 1102 64-65 0.21882661001617976 1102 66-67 0.15780172495888678 1102 68-69 0.19644958046571048 1102 70-71 -0.008703599475730073 1102 72-73 0.140999884956706 1102 74-75 0.15311900674513623 1102 76-77 0.14950348355426968 1102 78-79 0.16572064227958805 1102 80-81 0.10540850557943315 1102 82-83 0.19492397755469426 1102 84-85 0.07294956619542603 1102 86-87 0.03331346356862497 1102 88-89 -0.0027436453285432094 1102 90-91 0.04115506537792868 1102 92-93 0.14622795088286722 1102 94-95 0.07531387945881818 1102 96-97 0.11100924886518726 1102 98-99 0.18563414642266451 1102 100-101 0.1019589352050545 1102 102-103 0.19155830038378951 1102 104-105 0.1669403777120806 1102 106-107 0.050860786512984646 1102 108-109 0.021563788490610136 1102 110-111 0.07204605079776627 1102 112-113 -0.03966786641261422 1102 114-115 0.035868361674168625 1102 116-117 -0.08904850323311564 1102 118-119 0.02569231606758393 1102 120-121 0.01656595883636669 1102 122-123 -0.009373642226606194 1102 124-125 0.023403079424618056 1103 1 0.12243160300234734 1103 2 0.09752383293108124 1103 3 0.063968199010489 1103 4 0.060520985183607934 1103 5 0.07903565068745877 1103 6 0.0035381321691474454 1103 7 0.037129394012332284 1103 8 -0.01066900578266683 1103 9 0.10183889809356117 1103 10-11 0.09299010142969166 1103 12-13 0.1273165552767992 1103 14-15 0.15421245199510025 1103 16-17 0.18108553448534082 1103 18-19 0.2204362920473244 1103 20-21 0.1147769555508873 1103 22-23 0.18058128521452232 1103 24-25 0.12092634186934248 1103 26-27 0.21356176953187855 1103 28-29 0.17434683704935594 1103 30-31 0.19881453638612356 1103 32-33 0.2361698178831375 1103 34-35 0.23919375986621816 1103 36-37 0.13844613134735795 1103 38-39 0.09477613277824304 1103 40-41 0.29554007300113483 1103 42-43 0.2643954419394916 1103 44-45 0.3503288552322843 1103 46-47 0.2350115030442339 1103 48-49 0.25633399541870006 1103 50-51 0.24621550780731383 1103 52-53 0.20042636022478888 1103 54-55 0.3181010946567042 1103 56-57 0.4310447176850758 1103 58-59 0.27018006714197895 1103 60-61 0.2827757083071276 1103 62-63 0.3107113334761351 1103 64-65 0.2991688313624721 1103 66-67 0.34469354821128206 1103 68-69 0.2134885438352967 1103 70-71 0.1551534167989317 1103 72-73 0.22516842232017353 1103 74-75 0.13951940155428844 1103 76-77 0.13486317808991544 1103 78-79 0.1505227329735348 1103 80-81 0.1599968000647678 1103 82-83 0.10138112618015072 1103 84-85 0.14713330947973446 1103 86-87 0.21863902890360976 1103 88-89 0.2777078535522506 1103 90-91 0.27974621838410485 1103 92-93 0.20140108847802196 1103 94-95 0.20102630649363107 1103 96-97 0.18806673493710946 1103 98-99 0.11665791028064376 1103 100-101 0.13507467485533908 1103 102-103 0.23641817037236734 1103 104-105 0.18118090685889854 1103 106-107 0.154392437741393 1103 108-109 0.1808526192690607 1103 110-111 0.16238508354737746 1103 112-113 0.16415396313685449 1103 114-115 0.14229538687369825 1103 116-117 0.21446023496387312 1103 118-119 0.1640742074021304 1103 120-121 0.10266688192709239 1103 122-123 0.12319987575691727 1103 124-125 0.03532827078047873 1104 1 0.07110698571128893 1104 2 0.08050689735635785 1104 3 0.15522378474605603 1104 4 0.13735379488683463 1104 5 0.058722035887292634 1104 6 0.07725732456879086 1104 7 0.08185126506163698 1104 8 0.07948507915806147 1104 9 0.17135236018566502 1104 10-11 0.21135606255172235 1104 12-13 0.23117055635326977 1104 14-15 0.17854091202188727 1104 16-17 0.2167262825045171 1104 18-19 0.18813525062839176 1104 20-21 0.14135457380575644 1104 22-23 0.19851587734630272 1104 24-25 0.18991486998890394 1104 26-27 0.20846576372396441 1104 28-29 0.1667873184552775 1104 30-31 0.1773798628934955 1104 32-33 0.25302637869724265 1104 34-35 0.2745998131137668 1104 36-37 0.38443832696388114 1104 38-39 0.3985947731757875 1104 40-41 0.46086532866196706 1104 42-43 0.3670916154759283 1104 44-45 0.2623026695543018 1104 46-47 0.2545529720457438 1104 48-49 0.28359692240672985 1104 50-51 0.2551135689155828 1104 52-53 0.40165287510130554 1104 54-55 0.3561200830283582 1104 56-57 0.34549299721593485 1104 58-59 0.22539795776796012 1104 60-61 0.17260920018981807 1104 62-63 0.16212842050721576 1104 64-65 0.048630598019542504 1104 66-67 -0.04838811931071518 1104 68-69 -0.1902592187412111 1104 70-71 -0.03246158363916152 1104 72-73 -0.009700023437169136 1104 74-75 -0.07515355910460642 1104 76-77 -0.09556406027235198 1104 78-79 -0.19034951796837163 1104 80-81 -0.07596062943292736 1104 82-83 0.014939130210642304 1104 84-85 0.017199243064247582 1104 86-87 0.08638148424901715 1104 88-89 -0.025388709264589693 1104 90-91 0.009154099121602144 1104 92-93 0.13613951333188368 1104 94-95 0.1584401578661243 1104 96-97 0.15098261407243285 1104 98-99 0.09241394037164241 1104 100-101 0.16829337283133583 1104 102-103 0.07905767294462152 1104 104-105 0.08062906121921287 1104 106-107 0.0805496394286962 1104 108-109 0.08403398513004845 1104 110-111 0.0925938993088522 1104 112-113 0.035355819416039935 1104 114-115 0.044836036008142344 1104 116-117 0.07984557248624213 1104 118-119 0.16105212105949107 1104 120-121 0.12331767488250023 1104 122-123 -0.014047279742449348 1104 124-125 0.02199447848848024 1105 1 0.07280665567828493 1105 2 0.07752459912653364 1105 3 0.11180944331191256 1105 4 0.11405046455379875 1105 5 0.057427406424345406 1105 6 0.08005960479681562 1105 7 0.05927550748587862 1105 8 0.0907722078709341 1105 9 0.08406163111275333 1105 10-11 0.12726815376084488 1105 12-13 0.16892633193082673 1105 14-15 0.2448970476354546 1105 16-17 0.16544365424169172 1105 18-19 0.21655934303763047 1105 20-21 0.14138232658103078 1105 22-23 0.18540831659023382 1105 24-25 0.18575720422233388 1105 26-27 0.13085250239782908 1105 28-29 0.12438782850627916 1105 30-31 0.039252800187227876 1105 32-33 0.08858218427779718 1105 34-35 0.1473345865911142 1105 36-37 0.22271690482167372 1105 38-39 0.16625603930239663 1105 40-41 0.12604384651375256 1105 42-43 0.20373728004238245 1105 44-45 0.36275271455880187 1105 46-47 0.30298606535507844 1105 48-49 0.2515544681613022 1105 50-51 0.34728128568725225 1105 52-53 0.2897379336071566 1105 54-55 0.28529950097015444 1105 56-57 0.25437613552976757 1105 58-59 0.30306747471965423 1105 60-61 0.3730434936191571 1105 62-63 0.3723961972848997 1105 64-65 0.4199327282325598 1105 66-67 0.40442691219243443 1105 68-69 0.5208141385945169 1105 70-71 0.3270493674559489 1105 72-73 0.33723216978214765 1105 74-75 0.3778084871000189 1105 76-77 0.4089596420978836 1105 78-79 0.27312932991642214 1105 80-81 0.2759880654365574 1105 82-83 0.2563020164992764 1105 84-85 0.26171094423435903 1105 86-87 0.32624122022262014 1105 88-89 0.3246120408104147 1105 90-91 0.3369863823499344 1105 92-93 0.3214462940099594 1105 94-95 0.2448818020305481 1105 96-97 0.28936620243126754 1105 98-99 0.3606842674043449 1105 100-101 0.3837794214361949 1105 102-103 0.3863641535926874 1105 104-105 0.3866454128543779 1105 106-107 0.48065915037979323 1105 108-109 0.46272060379190805 1105 110-111 0.33234562448136984 1105 112-113 0.37550208404249474 1105 114-115 0.3004841008146215 1105 116-117 0.3101726051895142 1105 118-119 0.3181545813055138 1105 120-121 0.3376628594009503 1105 122-123 0.3228466596514963 1105 124-125 0.4098162606666982 1106 1 0.07133772912461467 1106 2 0.14984978806189275 1106 3 0.09442739033664083 1106 4 -0.028296002538432674 1106 5 0.03499142432734459 1106 6 0.08534832737367282 1106 7 0.1093936379429934 1106 8 0.16505792215664883 1106 9 0.11129913936721181 1106 10-11 0.23195815302710798 1106 12-13 0.26269550061524427 1106 14-15 0.2966785429762666 1106 16-17 0.3183822410785879 1106 18-19 0.27979582407071746 1106 20-21 0.2652622884270954 1106 22-23 0.3504424350141804 1106 24-25 0.27941484601556965 1106 26-27 0.21319750203096532 1106 28-29 0.2958193348530358 1106 30-31 0.34838920051006994 1106 32-33 0.3657954794572049 1106 34-35 0.3489561288906202 1106 36-37 0.3729326213085784 1106 38-39 0.44504611927891347 1106 40-41 0.40869334390900747 1106 42-43 0.502235335659087 1106 44-45 0.4192021972794677 1106 46-47 0.2805985094082004 1106 48-49 0.3500525237780039 1106 50-51 0.41418568072724327 1106 52-53 0.3786549487953579 1106 54-55 0.4283187980572407 1106 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-0.548772987960163 2216 70-71 -0.5223650873886854 2216 72-73 -0.49629563292759826 2216 74-75 -0.4899818278976369 2216 76-77 -0.41965129092771747 2216 78-79 -0.4656448719043311 2216 80-81 -0.44949084211568646 2216 82-83 -0.47193664657719125 2216 84-85 -0.35148433721197847 2216 86-87 -0.3865749527910154 2216 88-89 -0.4226807745077892 2216 90-91 -0.31571952011619686 2216 92-93 -0.37235889468945516 2216 94-95 -0.3115566302470967 2216 96-97 -0.25478603930912413 2216 98-99 -0.30603283307862483 2216 100-101 -0.3046447355070043 2216 102-103 -0.3579710175831039 2216 104-105 -0.3077709653137717 2216 106-107 -0.285163818376887 2216 108-109 -0.2300562479412207 2216 110-111 -0.31275930350084735 2216 112-113 -0.46938956873349724 2216 114-115 -0.3690696881326758 2216 116-117 -0.4060675110647054 2216 118-119 -0.3831977288197166 2216 120-121 -0.40724674439440633 2216 122-123 -0.378028238839768 2216 124-125 -0.43437273792286746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 1.0 4 13.0 5 27.0 6 86.0 7 180.0 8 315.0 9 531.0 10 912.0 11 1358.0 12 1821.0 13 2870.0 14 5111.0 15 5446.0 16 2643.0 17 2143.0 18 2012.0 19 1649.0 20 1393.0 21 1224.0 22 1226.0 23 1349.0 24 1607.0 25 2045.0 26 2735.0 27 3177.0 28 3863.0 29 4780.0 30 5816.0 31 7189.0 32 9247.0 33 11928.0 34 18186.0 35 34175.0 36 88891.0 37 259265.0 38 148502.0 39 1181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41653801803391 17.903636398006313 13.555365120572649 23.124460463387127 2 15.920943411977252 19.195226867143287 40.56107622301292 24.32275349786655 3 19.815907727055798 24.41396190575194 28.449564418907574 27.320565948284685 4 12.924695840338652 15.917872948479753 36.124578497954396 35.0328527132272 5 13.877504715175066 37.46850641216539 32.834478053361444 15.819510819298104 6 32.56666414628307 35.6794954192028 16.57278237747785 15.181058057036282 7 29.295072310372312 32.650235784420275 19.571996938110132 18.482694967097284 8 27.302953841695256 34.72047654598662 18.513565873158377 19.463003739159745 9 27.52755543375198 16.034758120150826 19.172880224034575 37.26480622206262 10-11 26.07544156420991 26.272794856529043 25.698926763500506 21.95283681576054 12-13 26.91801254366816 23.416291018141383 26.974084189370956 22.691612248819503 14-15 23.409675823985435 24.346040806297665 25.686720155236557 26.557563214480346 16-17 22.745557582115037 27.9873114275904 26.07126769170675 23.195863298587813 18-19 22.810370734381056 25.459362232281517 29.25293982378383 22.4773272095536 20-21 23.428103864848435 24.700662464443333 29.64063745270924 22.230596217999 22-23 23.109550765314964 24.57458001392341 29.62803708288838 22.687832137873247 24-25 22.76595443076254 24.90919858497847 29.607403977306735 22.717443006952255 26-27 22.41629416823384 25.358874282960205 29.50352967859607 22.72130187020989 28-29 22.353852853876873 25.35417022694143 29.664379953047685 22.62759696613401 30-31 22.566904131510267 24.936407408399106 29.679666593689408 22.81702186640122 32-33 22.464648087421367 25.01897930704266 29.579525659078094 22.936846946457877 34-35 22.927777375263428 25.24728381566803 29.17098494561331 22.653953863455232 36-37 23.240506448739666 24.904964076539063 29.122298849488455 22.732230625232816 38-39 22.729141366649813 25.075880361831587 29.104273084247286 23.090705187271315 40-41 22.906817682455756 24.84531040690628 29.322074876299514 22.92579703433845 42-43 23.0610997706711 24.799494720395153 28.91300307451929 23.226402434414457 44-45 22.937385217783014 25.15845064876563 28.9092893661659 22.994874767285452 46-47 23.403296886763627 25.577805708597545 28.065669977413837 22.95322742722499 48-49 23.482840876963216 25.540502334636173 27.797094167465353 23.179562620935258 50-51 23.436723539811858 25.94541595624456 27.558523687080882 23.059336816862697 52-53 23.69034906174496 25.623245792264004 27.57157797581359 23.114827170177445 54-55 23.21547262412152 25.753580867598462 27.59685746776668 23.43408904051334 56-57 23.365002661861663 26.156334758243112 27.442454960985614 23.036207618909614 58-59 23.44224002419332 25.514344307481974 27.559908393002843 23.48350727532187 60-61 23.19731293092925 25.68663198888001 28.051993053942205 23.06406202624854 62-63 23.08834218905167 25.69182100187352 28.076997891795642 23.142838917279164 64-65 22.906919327668884 25.57594123757374 27.977438806858768 23.539700627898608 66-67 22.948858593487284 25.923359161690136 27.621588715641053 23.50619352918153 68-69 22.974822608462816 25.595334661636965 28.067318217973053 23.36252451192717 70-71 23.08373221944485 25.667317169979825 27.792399664195383 23.45655094637994 72-73 23.08789387968537 25.867456709854437 27.501724675619226 23.542924734840966 74-75 23.075154177459023 26.255411272457224 27.209417523820623 23.46001702626313 76-77 23.368046175982535 25.948079584107436 27.161624601988894 23.522249637921135 78-79 23.24673903028136 26.06751819787666 27.285356409046845 23.400386362795135 80-81 23.26870918661463 26.340915605873032 27.20207213081704 23.188303076695302 82-83 23.44361807019036 26.228536057533287 27.07858850657267 23.24925736570368 84-85 23.43823461545428 26.089603298795005 26.978643042943084 23.49351904280763 86-87 23.375135257733906 26.01461019784249 27.198105525445783 23.41214901897782 88-89 23.334231109683067 26.31272227839887 26.910216064841503 23.442830547076557 90-91 23.3741585315529 26.364620051598514 27.083156140632724 23.178065276215857 92-93 23.301672004807084 25.765301903538607 27.28531343297687 23.647712658677438 94-95 23.24360721064113 25.821435040439756 27.279943927736085 23.65501382118303 96-97 23.433588203468744 25.787271508752973 27.14055868772725 23.63858160005103 98-99 23.183971699569383 25.84540606266794 26.87036739528305 24.100254842479625 100-101 22.981844356346443 25.799142855792812 27.265517822519804 23.953494965340944 102-103 23.149241930250653 25.260827655291685 27.38083987765041 24.209090536807253 104-105 23.00236886952632 25.583869636573837 27.15749832257577 24.25626317132408 106-107 23.15128366671917 25.71326193101276 26.932036541187585 24.203417861080485 108-109 23.36964097410846 25.502186569979298 26.89280255032891 24.235369905583333 110-111 23.001108832544233 25.787995627671673 27.045040021924642 24.16585551785945 112-113 23.03886129919877 25.76921683606369 27.1632968393398 24.02862502539774 114-115 22.850325055029 25.78145119055588 27.007485519202067 24.36073823521305 116-117 22.791177151569467 25.717793594866567 27.24221409141955 24.24881516214441 118-119 22.820855899240748 25.945208651132972 26.7934531370713 24.440482312554977 120-121 22.778572739235926 26.111215676762455 26.99686486801496 24.11334671598665 122-123 22.694500949361355 25.922014551886473 26.99438730950755 24.389097189244612 124-125 22.73423164460265 25.962403232030496 26.8486127627403 24.45475236062656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 105.0 1 80.5 2 174.0 3 287.5 4 179.0 5 117.0 6 331.5 7 872.5 8 1547.0 9 1696.0 10 1171.0 11 684.5 12 590.5 13 647.5 14 726.0 15 797.0 16 849.0 17 927.5 18 1002.0 19 1069.0 20 1191.5 21 1279.5 22 1447.0 23 1824.5 24 2200.0 25 2572.5 26 3094.0 27 3697.5 28 4242.5 29 4991.0 30 5860.5 31 6823.5 32 8126.0 33 8978.0 34 9600.0 35 10242.5 36 11385.5 37 12950.5 38 13760.5 39 14070.0 40 14633.0 41 15824.5 42 16981.0 43 17990.5 44 19174.5 45 20691.0 46 21641.0 47 21982.0 48 22693.5 49 23008.5 50 23061.0 51 23502.5 52 24123.0 53 25272.5 54 26066.5 55 25208.0 56 23151.0 57 21072.0 58 19660.5 59 17616.0 60 15024.0 61 12577.5 62 10257.0 63 7981.5 64 6106.0 65 4777.5 66 3391.0 67 2664.5 68 1996.0 69 1210.5 70 851.5 71 623.0 72 501.0 73 440.0 74 358.0 75 270.5 76 180.5 77 87.5 78 49.0 79 29.0 80 12.0 81 7.0 82 6.0 83 4.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015435453030546446 2 0.0023625693414101702 3 4.725138682820341E-4 4 0.1354539755741831 5 0.03921865106740883 6 0.012285360575332888 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.93761556901695E-4 30-31 1.5750462276067803E-4 32-33 0.0 34-35 6.300184910427121E-4 36-37 0.0016537985389871192 38-39 0.0033075970779742384 40-41 2.3625693414101704E-4 42-43 9.450277365640682E-4 44-45 6.300184910427121E-4 46-47 0.0 48-49 0.0010237800479444073 50-51 0.0014962939162264414 52-53 0.0 54-55 0.0 56-57 0.0012600369820854242 58-59 0.0025200739641708485 60-61 0.0014962939162264414 62-63 2.3625693414101704E-4 64-65 0.0010237800479444073 66-67 8.662754251837292E-4 68-69 0.0011025323593247462 70-71 0.0014175416048461023 72-73 0.0 74-75 7.875231138033901E-5 76-77 0.004173872503157967 78-79 0.0010237800479444073 80-81 0.0 82-83 0.0 84-85 5.512661796623731E-4 86-87 4.725138682820341E-4 88-89 0.0 90-91 0.0 92-93 8.662754251837292E-4 94-95 0.0011025323593247462 96-97 5.512661796623731E-4 98-99 0.0 100-101 4.725138682820341E-4 102-103 0.0 104-105 0.0 106-107 3.1500924552135606E-4 108-109 5.512661796623731E-4 110-111 0.0 112-113 4.725138682820341E-4 114-115 0.0014962939162264414 116-117 7.8752311380339E-4 118-119 3.93761556901695E-4 120-121 7.875231138033901E-5 122-123 2.3625693414101704E-4 124-125 0.0011025323593247462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 634902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.29356862162246 #Duplication Level Percentage of deduplicated Percentage of total 1 77.39602453558346 45.1169046730581 2 12.845614478631282 14.976334181939993 3 4.205919331216687 7.355341414538651 4 1.8139613943168078 4.229691320663232 5 0.9781130246668863 2.850884936156092 6 0.6194495053547837 2.1665953348817504 7 0.41689808214037977 1.7011733871631116 8 0.2822085875788146 1.3160756532509426 9 0.23038001877705339 1.208670609026775 >10 1.1208420940489598 11.59984101561814 >50 0.059761694782396055 2.3861126520361626 >100 0.02866393690930238 3.3284474341308252 >500 0.0013520724957151225 0.49891243802969176 >1k 8.112434974290735E-4 1.2650149495065643 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3665 0.5772544424178849 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2683 0.4225849028668991 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.26413525236965707 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 0.12789375368167055 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.5750462276067803E-4 14-15 0.0 0.0 0.0 0.0 1.5750462276067803E-4 16-17 0.0 0.0 0.0 0.0 1.5750462276067803E-4 18-19 0.0 0.0 0.0 0.0 1.5750462276067803E-4 20-21 0.0 0.0 0.0 0.0 1.5750462276067803E-4 22-23 0.0 0.0 0.0 0.0 1.5750462276067803E-4 24-25 0.0 0.0 0.0 1.5750462276067803E-4 1.5750462276067803E-4 26-27 0.0 0.0 0.0 2.3625693414101704E-4 1.5750462276067803E-4 28-29 0.0 0.0 0.0 0.0011025323593247462 1.5750462276067803E-4 30-31 0.0 0.0 0.0 0.0025200739641708485 1.5750462276067803E-4 32-33 0.0 0.0 0.0 0.005985175664905765 1.5750462276067803E-4 34-35 0.0 0.0 0.0 0.010710314347726107 3.1500924552135606E-4 36-37 0.0 0.0 0.0 0.01976683015646509 3.1500924552135606E-4 38-39 0.0 0.0 0.0 0.03173718148627662 3.1500924552135606E-4 40-41 0.0 0.0 0.0 0.048511423810288834 3.1500924552135606E-4 42-43 0.0 0.0 0.0 0.06599443693672409 3.1500924552135606E-4 44-45 0.0 0.0 0.0 0.08717880869803529 3.1500924552135606E-4 46-47 0.0 0.0 0.0 0.11253705296250445 3.1500924552135606E-4 48-49 0.0 0.0 0.0 0.139155334209059 3.1500924552135606E-4 50-51 0.0 0.0 0.0 0.16986873564739124 3.1500924552135606E-4 52-53 0.0 0.0 0.0 0.20073964170848413 3.1500924552135606E-4 54-55 0.0 0.0 0.0 0.2337368601768462 3.1500924552135606E-4 56-57 0.0 0.0 0.0 0.2712229603938876 3.1500924552135606E-4 58-59 0.0 0.0 0.0 0.3183955949107106 3.1500924552135606E-4 60-61 0.0 0.0 0.0 0.3717896620265805 3.1500924552135606E-4 62-63 0.0 0.0 0.0 0.42510497683106996 3.1500924552135606E-4 64-65 0.0 0.0 0.0 0.47731775927623477 3.1500924552135606E-4 66-67 0.0 0.0 0.0 0.5399258468236043 3.1500924552135606E-4 68-69 0.0 0.0 0.0 0.613480505652841 3.1500924552135606E-4 70-71 0.0 0.0 0.0 0.6957766710452952 3.1500924552135606E-4 72-73 0.0 0.0 0.0 0.7796478826653561 3.1500924552135606E-4 74-75 0.0 0.0 0.0 0.8674567098544342 3.1500924552135606E-4 76-77 0.0 0.0 0.0 0.9699134669602553 4.725138682820341E-4 78-79 0.0 0.0 0.0 1.0814267398748154 4.725138682820341E-4 80-81 0.0 0.0 0.0 1.2123918337003192 5.512661796623731E-4 82-83 0.0 0.0 0.0 1.3502871309272928 7.875231138033901E-4 84-85 0.0 0.0 0.0 1.4858198588128562 7.875231138033901E-4 86-87 0.0 0.0 0.0 1.6271802577405647 0.0010237800479444073 88-89 0.0 0.0 0.0 1.7915363315913322 0.0012600369820854242 90-91 0.0 0.0 0.0 1.9974736258509187 0.0012600369820854242 92-93 0.0 0.0 0.0 2.204985966338112 0.0014175416048461023 94-95 0.0 0.0 0.0 2.4102144897952753 0.0014175416048461023 96-97 0.0 0.0 0.0 2.61567927018658 0.0017325508503674581 98-99 0.0 0.0 0.0 2.8497311396089477 0.002047560095888814 100-101 0.0 0.0 0.0 3.124734210949091 0.002047560095888814 102-103 0.0 0.0 0.0 3.4237567372602387 0.002047560095888814 104-105 0.0 0.0 0.0 3.718447886445467 0.002047560095888814 106-107 0.0 0.0 0.0 4.008413896947875 0.002047560095888814 108-109 0.0 0.0 0.0 4.3196430315229755 0.002047560095888814 110-111 0.0 0.0 0.0 4.685053756327749 0.002047560095888814 112-113 0.0 0.0 0.0 5.065663677228927 0.002047560095888814 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 70.25086 1 GTATCAA 3150 0.0 58.26666 1 TATCAAC 3905 0.0 46.83759 2 ATCAACG 3940 0.0 46.723946 3 TCAACGC 4005 0.0 45.81687 4 CAACGCA 4075 0.0 45.129772 5 AACGCAG 4140 0.0 44.407215 6 GTGGTAT 655 0.0 42.759884 1 ACGCAGA 4815 0.0 38.058342 7 CGCAGAG 4910 0.0 37.32197 8 ATAACGC 85 1.6967056E-4 35.04518 3 TGGTATC 770 0.0 34.817616 2 GCAGAGT 5360 0.0 34.077602 9 TAACGCA 70 0.0027704719 34.043888 4 GTACATG 3595 0.0 32.3233 1 ACATGGG 3580 0.0 31.951807 3 TACATGG 3615 0.0 31.80726 2 GTTCGCA 80 0.0053205555 29.795452 1 CTATTAC 140 3.0549654E-6 29.79545 1 GAGTACT 3440 0.0 28.45135 12-13 >>END_MODULE