FastQCFastQC Report
Fri 27 May 2016
SRR940084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770987
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.046.7211761
GTATCAA13350.046.029311
TCAACGC14200.042.3868564
GTACATG34500.041.502151
CAACGCA14700.040.934485
ATCAACG14850.040.531543
TACATGG35250.039.429272
AACGCAG15550.038.6969036
ACATGGG36450.036.6225553
TATCAAC16800.036.57212
CATGGGG21950.030.950564
ACGCAGA19750.030.1660277
CGCAGAG19800.029.4880548
CATGGGA18750.027.3335064
ATGGGGA13000.027.0392045
TTATACG1451.3253269E-424.6593364
GCAGAGT24000.023.823429
GTGTAGC4400.023.0502031
GTAAGGT3900.022.9205384
GTATAGC3201.1785232E-820.50791
Produced by FastQC (version 0.11.5)