FastQCFastQC Report
Fri 27 May 2016
SRR940084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770987
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.3394350358696061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.26213152750954294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.15486642446630097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.062.9845351
GTATCAA30300.055.2939381
ATCAACG35650.046.8135153
TCAACGC36200.046.1022574
CAACGCA36900.045.1689955
TATCAAC37950.044.2904472
AACGCAG37950.043.9050146
ACGCAGA43650.038.1642847
CGCAGAG44600.037.0845768
GCAGAGT49850.033.1789789
GTACATG35850.033.0961721
GTGGTAT8000.032.7926521
TACATGG35200.032.511072
ACATGGG35850.030.5915453
GAGTACT31500.029.55882312-13
TGGTATC8650.028.9404932
CAGAGTA48900.027.4968510-11
GTACTTT34200.027.0512714-15
CATGGGG19200.026.076594
AGAGTAC47250.025.81281510-11
Produced by FastQC (version 0.11.5)