FastQCFastQC Report
Fri 27 May 2016
SRR940083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834691
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG300.0041949259.6471141
GTATCAA16900.052.588281
GGTATCA10850.045.6286661
TCAACGC19250.043.978284
ATCAACG19250.043.9756363
CAACGCA19950.042.4097065
AACGCAG20550.041.1714636
TATCAAC22800.038.1973762
GTACATG46550.035.2372861
TACATGG46800.034.1589472
ACGCAGA26800.031.569917
ACATGGG48350.031.5644363
CGCAGAG26650.031.5240278
CATGGGG21350.029.3205474
GTGTAGC5750.026.9708671
GCAGAGT31550.025.858689
CATGGGA27950.025.3831654
TCGAACG851.4053539E-524.4862552-53
CGTTACG751.6910673E-423.791632108-109
GAGTACA34050.022.422411
Produced by FastQC (version 0.11.5)