FastQCFastQC Report
Fri 27 May 2016
SRR940083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834691
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40500.48520949668799596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.35210634833728893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.21301295928673006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.078.946861
GTATCAA35200.058.610171
ATCAACG43850.047.176013
TCAACGC44500.046.486924
TATCAAC45100.045.7362862
CAACGCA45450.045.4388775
AACGCAG46900.044.147726
ACGCAGA54200.037.9797977
CGCAGAG55600.036.9164738
GTACATG44800.033.140641
GCAGAGT62650.032.6672979
TGGTATC7150.031.683922
TACATGG46700.031.6589222
ACATGGG45350.031.4182453
GTAAGGT3050.031.273864
GTGGTAT7450.031.213891
CATGGGA26750.029.8636134
GAGTACT39550.028.80700912-13
ACAACGC1254.8167538E-528.6155823
CAGAGTA61300.027.75756810-11
Produced by FastQC (version 0.11.5)