FastQCFastQC Report
Fri 27 May 2016
SRR940082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441539
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC6450.048.0459064
GTATCAA6600.047.900411
GGTATCA4550.047.195261
ATCAACG6850.045.2403073
CAACGCA7200.043.0264745
AACGCAG7600.040.761926
GTACATG15050.039.2379231
TATCAAC8350.037.85712
ACATGGG15550.036.792023
TACATGG15950.036.645732
ACGCAGA9450.034.0429277
CGCAGAG9400.032.9564488
CATGGGG11100.030.066154
TCCGCGA400.00537305429.73694426-27
GTGTAGG3250.029.3659421
GTATTAG1903.617788E-828.2550581
TCTATAC1505.2192227E-627.8111883
GCAGAGT11400.027.6814989
GGGCGTA900.00949053126.4628169
TAGGATC2001.7963794E-623.8381614
Produced by FastQC (version 0.11.5)