FastQCFastQC Report
Fri 27 May 2016
SRR940082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441539
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.3782225352686852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.2919334418930151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.16895449779068214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.072.946421
GTATCAA15650.060.5367851
TCAACGC18250.049.615664
ATCAACG18450.049.4007033
CAACGCA18650.048.507525
TATCAAC19450.046.8608252
AACGCAG20050.045.105136
ACGCAGA22750.039.751997
CGCAGAG22950.039.405578
CGCTATA650.001926225436.6593482
GTACATG15900.035.975821
GTATAGG1051.4672167E-534.0485461
GCAGAGT27000.033.494739
TACATGG16950.033.0366672
ACCGTCC750.00390879331.7318538
ACATGGG17100.031.3533883
GTGGTAT3250.031.1675131
TGGTATC3600.029.785722
GCACCGT1454.060912E-628.7227976
GAGTACT19950.028.48112912-13
Produced by FastQC (version 0.11.5)