FastQCFastQC Report
Fri 27 May 2016
SRR940081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484532
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53891.1122072432780497No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT15680.3236112372351052No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.21773587709377298No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT9410.19420801928458803No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9200.189873940214475No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG9080.18739732360298184No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8850.18265047509761997No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8840.18244409037999554No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA8810.18182493622712223No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8140.1679971601462855No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT7860.16221838805280145No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC7740.15974177144130833No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT7650.15788430898268846No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7540.15561407708881975No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.14818422725434027No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC7100.14653314951334484No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG6690.13807137609074324No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6520.13456283589112794No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC6460.13332452758538135No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6310.13022875682101492No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA6200.12795852492714618No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG5680.11722651961067587No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5520.11392436412868501No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG5490.11330520997581171No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT5420.1118605169524407No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT5410.11165413223481627No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA5340.11020943921144527No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT5240.10814559203520097No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5160.10649451429420555No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4980.10277958937696581No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4920.10154128107121924No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA4900.10112851163597038No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4880.10071574220072152No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT4870.10050935748309708No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC4850.10009658804784824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.068.6759341
GTATCAA11350.054.1727641
GAACAAA6700.048.112581
GTTATAT802.2400327E-644.7714231
ATCAACG13350.043.3291633
TCAACGC13600.042.5326734
GTACATG31000.041.59411
CAACGCA14050.041.581945
ATATAGG6300.039.796822
AACGCAG14700.038.9321986
TGGGTAT2200.037.9367686
CATATAG957.3494484E-637.6632233
CTTACTA6050.037.420859
GGGTATG2400.037.2593277
CTGCTCG650.001925378836.6633389
CATGGGT3200.035.4073524
ACATGGG33800.035.1096273
TACATGG35850.034.634892
GGTACTC700.00276115134.065678
GGATGAC3750.033.3843578
Produced by FastQC (version 0.11.5)