FastQCFastQC Report
Fri 27 May 2016
SRR940081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484532
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99902.0617833290680494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68481.4133225462920922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47340.9770252532340487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.3586966392312582No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT16630.343217785409426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.3399156299274351No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.3029727654726623No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.28666837278033236No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT9050.18677816945010856No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG8830.18223770566237113No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8770.18099939735662454No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8290.17109293091065195No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8150.1682035448639099No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA8000.1651077740995435No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC7820.16139284918230376No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT7390.1525183063244533No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT7320.15107361330108227No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7120.14694591894859368No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7040.14529484120759825No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC6830.14096076213748523No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6770.13972245383173867No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC6400.1320862192796348No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA6360.13126068040913705No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.12816490964477062No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG6200.12795852492714618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.12754575549189734No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG5990.12362444585703317No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA5540.11433713356393387No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT5530.11413074884630943No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA5500.11351159469343614No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5390.11124136279956742No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5370.11082859336431856No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.11041582392906969No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5190.10711366844707883No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG5080.10484343655321011No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC5040.10401789768271238No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4970.10257320465934139No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT4890.10092212691834594No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4850.10009658804784824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG355.4569682E-11119.246052
GGTATCA19800.092.766111
ATCACGC453.9835868E-1092.7469253
CCGTTGC304.2639163E-579.325079
TAACGCA551.9572326E-975.883854
TCACGCA603.8962753E-969.560194
CACGCAG603.936293E-969.473975
ATGGGCG359.109783E-568.056135
GTATCAA43100.064.4781341
TATAACG657.3378033E-964.2094042
TCCGTGC350.00779675350.9946948
GTGGTAT6000.050.6900561
ATCAACG55300.050.3507273
TCAACGC56200.049.54444
CAACGCA56600.049.238495
GTATGGT751.4462148E-647.708291
TATCAAC58500.047.698422
CATATAG1251.0913936E-1147.6984183
AACGCAG58400.047.5795866
GTATCAC909.536416E-846.3830531
Produced by FastQC (version 0.11.5)