##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940081_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 484532 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.10759041714479 34.0 33.0 34.0 31.0 34.0 2 33.142411646702385 34.0 33.0 34.0 31.0 34.0 3 33.195809564693356 34.0 33.0 34.0 31.0 34.0 4 36.44132688862655 37.0 37.0 37.0 35.0 37.0 5 36.479039568078065 37.0 37.0 37.0 35.0 37.0 6 36.577825613169 37.0 37.0 37.0 35.0 37.0 7 36.56403705018451 37.0 37.0 37.0 35.0 37.0 8 36.57559872206583 37.0 37.0 37.0 35.0 37.0 9 38.45074215944458 39.0 39.0 39.0 37.0 39.0 10-11 38.42983848331999 39.0 39.0 39.0 37.0 39.0 12-13 38.417050886215975 39.0 39.0 39.0 37.0 39.0 14-15 39.929368958087395 41.0 40.0 41.0 38.0 41.0 16-17 39.955670626501444 41.0 40.0 41.0 38.0 41.0 18-19 40.09383900340947 41.0 40.0 41.0 38.0 41.0 20-21 40.10659770665302 41.0 40.0 41.0 38.0 41.0 22-23 40.01266583012061 41.0 40.0 41.0 38.0 41.0 24-25 39.98813700643095 41.0 40.0 41.0 38.0 41.0 26-27 39.86558679302915 41.0 40.0 41.0 37.0 41.0 28-29 39.76393715998118 41.0 40.0 41.0 37.0 41.0 30-31 39.48491946868319 41.0 39.0 41.0 35.5 41.0 32-33 39.15718260094276 41.0 39.0 41.0 35.0 41.0 34-35 39.00011970313622 41.0 39.0 41.0 35.0 41.0 36-37 38.86851436024865 41.0 39.0 41.0 35.0 41.0 38-39 38.74301800500277 41.0 38.0 41.0 35.0 41.0 40-41 38.598242221359996 41.0 38.0 41.0 35.0 41.0 42-43 38.3913239992405 41.0 38.0 41.0 35.0 41.0 44-45 38.082417673136135 41.0 38.0 41.0 35.0 41.0 46-47 37.599718904014594 41.0 38.0 41.0 33.5 41.0 48-49 37.162880882996376 41.0 38.0 41.0 33.0 41.0 50-51 37.14809135413141 41.0 38.0 41.0 33.0 41.0 52-53 37.104039568078065 41.0 38.0 41.0 33.0 41.0 54-55 37.00621630769484 41.0 38.0 41.0 33.0 41.0 56-57 36.88271672459197 41.0 37.0 41.0 33.0 41.0 58-59 36.721429131615665 41.0 37.0 41.0 32.0 41.0 60-61 36.57383413273014 40.0 36.5 41.0 32.0 41.0 62-63 36.34395251500417 40.0 36.0 41.0 31.0 41.0 64-65 36.09392568499088 39.5 35.5 41.0 31.0 41.0 66-67 35.81149851815773 39.0 35.0 41.0 31.0 41.0 68-69 35.48051625073266 39.0 35.0 41.0 30.5 41.0 70-71 35.134361610791444 37.5 35.0 40.5 30.0 41.0 72-73 34.764236417821735 37.0 35.0 39.5 29.0 41.0 74-75 34.37256672417921 37.0 35.0 39.0 29.0 41.0 76-77 33.99889996945506 36.0 35.0 39.0 29.0 41.0 78-79 33.62989441357846 36.0 35.0 38.0 28.5 39.5 80-81 33.255973805651635 35.5 35.0 37.0 28.0 39.0 82-83 32.90490204981302 35.0 35.0 37.0 26.5 39.0 84-85 32.56147065622085 35.0 35.0 36.0 26.0 38.0 86-87 32.3279556355411 35.0 35.0 36.0 26.0 37.0 88-89 32.14278107534693 35.0 35.0 36.0 26.0 37.0 90-91 32.04975625964849 35.0 35.0 36.0 26.0 36.5 92-93 31.92185655436586 35.0 35.0 35.0 25.0 36.0 94-95 31.80062823508045 35.0 35.0 35.0 25.0 36.0 96-97 31.689499351951987 35.0 35.0 35.0 24.5 36.0 98-99 31.618049994634 35.0 35.0 35.0 24.0 36.0 100-101 31.559317031692437 35.0 35.0 35.0 23.5 36.0 102-103 31.100865371121 35.0 33.5 35.0 20.0 35.0 104-105 31.43525505023404 35.0 34.5 35.0 20.0 35.0 106-107 31.446289409161828 35.0 35.0 35.0 20.0 35.5 108-109 31.426126654173512 35.0 35.0 35.0 19.0 35.5 110-111 31.359724435125027 35.0 35.0 35.0 18.0 35.5 112-113 31.319588799088606 35.0 35.0 35.0 14.0 35.5 114-115 31.26053903560549 35.0 35.0 35.0 4.5 35.5 116-117 31.19705406454063 35.0 35.0 35.0 2.0 35.5 118-119 31.133773414346216 35.0 35.0 35.0 2.0 35.0 120-121 31.040358531531457 35.0 34.5 35.0 2.0 35.0 122-123 30.954513840159166 35.0 34.0 35.0 2.0 35.5 124-125 30.062405578991687 34.5 32.5 35.0 2.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.058847715501258335 1101 2 0.15321061391263413 1101 3 0.09639727152095645 1101 4 0.06801906385722845 1101 5 0.06035907060426382 1101 6 0.13038974494507016 1101 7 0.08564582321054814 1101 8 0.11219395754227435 1101 9 0.09417350513764688 1101 10-11 0.0916848125344174 1101 12-13 0.1084182823045623 1101 14-15 0.10327326322897079 1101 16-17 0.10558456783368086 1101 18-19 0.12184311269609793 1101 20-21 0.12864415488751035 1101 22-23 0.10988016192643357 1101 24-25 0.19571240959998448 1101 26-27 0.15708094181440657 1101 28-29 0.13750964358876416 1101 30-31 0.19655839795329655 1101 32-33 0.3505017286548622 1101 34-35 0.15585810776244813 1101 36-37 0.2723407575783483 1101 38-39 0.2867840100270058 1101 40-41 0.366148276673556 1101 42-43 0.325201334027895 1101 44-45 0.4277498266881139 1101 46-47 0.6192083351673716 1101 48-49 0.5975346775669692 1101 50-51 0.6076063831685588 1101 52-53 0.6652142798180876 1101 54-55 0.6941269323434156 1101 56-57 0.7645034912642359 1101 58-59 0.7729404766167249 1101 60-61 0.7270671506676436 1101 62-63 0.747239208061842 1101 64-65 0.8270149913640168 1101 66-67 0.8662154951233418 1101 68-69 0.9319063491142927 1101 70-71 0.8510271847623301 1101 72-73 0.7344742627322418 1101 74-75 0.686702468649365 1101 76-77 0.695657911419822 1101 78-79 0.6185938732093206 1101 80-81 0.6327314266967434 1101 82-83 0.673050143484275 1101 84-85 0.7334045749742657 1101 86-87 0.6981449939783104 1101 88-89 0.5698719850338492 1101 90-91 0.5794938857352392 1101 92-93 0.7258258736364738 1101 94-95 0.8016744145848591 1101 96-97 0.5860650913924488 1101 98-99 0.5482078032830735 1101 100-101 0.5981397866037987 1101 102-103 0.6194016276566856 1101 104-105 0.680681883917817 1101 106-107 0.6162050785508555 1101 108-109 0.6136155326499626 1101 110-111 0.6698815822991726 1101 112-113 0.7412824877296202 1101 114-115 0.6438197502446847 1101 116-117 0.6585152688196381 1101 118-119 0.5876995232603619 1101 120-121 0.6443094758074217 1101 122-123 0.7077036666604215 1101 124-125 0.6700271714325261 1102 1 0.19980520025609394 1102 2 0.23001142660756813 1102 3 0.26695191869207235 1102 4 0.15187959307915122 1102 5 0.15163315824861456 1102 6 0.142717140012401 1102 7 0.2110285574381905 1102 8 0.14465039075889052 1102 9 0.13456855249253152 1102 10-11 0.17595910848891805 1102 12-13 0.17489062128509403 1102 14-15 0.2534206497926803 1102 16-17 0.17349367612357014 1102 18-19 0.14040411760707627 1102 20-21 0.18150499514787555 1102 22-23 0.12932719835788475 1102 24-25 0.09702190996951998 1102 26-27 0.1733833510782219 1102 28-29 0.13483585442716617 1102 30-31 -0.01265062273218831 1102 32-33 -0.12504893779493642 1102 34-35 -0.033464427685942155 1102 36-37 -0.13433058893274108 1102 38-39 0.028315346436478706 1102 40-41 -0.1240540124552183 1102 42-43 -0.08580600248431125 1102 44-45 -0.18788941437809825 1102 46-47 -0.17528942335836462 1102 48-49 -0.23193921089863778 1102 50-51 -0.04288200425407496 1102 52-53 -0.19814995760982157 1102 54-55 -0.34407229776510206 1102 56-57 -0.19850005571541374 1102 58-59 -0.16538830528254778 1102 60-61 -0.1889610311271852 1102 62-63 -0.11515767128092591 1102 64-65 -0.15654848187953974 1102 66-67 -0.24252525576339679 1102 68-69 -0.2450417074661715 1102 70-71 -0.2922973310592454 1102 72-73 -0.27581426109072993 1102 74-75 -0.48348977593528275 1102 76-77 -0.5419412142482614 1102 78-79 -0.4477771642608275 1102 80-81 -0.6126450867620932 1102 82-83 -0.5196695595168137 1102 84-85 -0.4241035790839405 1102 86-87 -0.42050190319922365 1102 88-89 -0.387812519259338 1102 90-91 -0.3745637391295702 1102 92-93 -0.3810971571905739 1102 94-95 -0.3751138424299114 1102 96-97 -0.4182066934260149 1102 98-99 -0.5281221714058191 1102 100-101 -0.49535437357814516 1102 102-103 -0.37073645628944973 1102 104-105 -0.4545684734910296 1102 106-107 -0.36315794896606235 1102 108-109 -0.3209308506007602 1102 110-111 -0.36088839478671275 1102 112-113 -0.31015442425792017 1102 114-115 -0.35501884934040717 1102 116-117 -0.3564259905721556 1102 118-119 -0.27966683721892593 1102 120-121 -0.35903347403548835 1102 122-123 -0.220952961169246 1102 124-125 -0.18573802593420297 1103 1 0.07374046431061032 1103 2 0.06987514040654474 1103 3 0.16133011289991117 1103 4 -0.026996045762416543 1103 5 0.06645428260977582 1103 6 0.10864558975686123 1103 7 0.09007455403103393 1103 8 0.12080030558001909 1103 9 0.14819717259474885 1103 10-11 0.20491992620612365 1103 12-13 0.18085314257981366 1103 14-15 0.21509015575520607 1103 16-17 0.29700304579989023 1103 18-19 0.28180105116755527 1103 20-21 0.24198199685145028 1103 22-23 0.26731697689280765 1103 24-25 0.2563064074141508 1103 26-27 0.32329817220257695 1103 28-29 0.24301302648849088 1103 30-31 0.3246577247976248 1103 32-33 0.3008454403992715 1103 34-35 0.26636521456278217 1103 36-37 0.2804905354284202 1103 38-39 0.13989967352336663 1103 40-41 0.1553326996401836 1103 42-43 0.21232006225164213 1103 44-45 0.22096918868835047 1103 46-47 0.12624379640313066 1103 48-49 0.19480695605195564 1103 50-51 0.19135990375274048 1103 52-53 0.23881767441743307 1103 54-55 0.12015257446658012 1103 56-57 0.19247098346687608 1103 58-59 0.1472181683120013 1103 60-61 0.15430983769734752 1103 62-63 0.2604811702863685 1103 64-65 0.2633833750199486 1103 66-67 0.2395871803524443 1103 68-69 0.2992513078660224 1103 70-71 0.37976399773120306 1103 72-73 0.3315111222142093 1103 74-75 0.290786203621785 1103 76-77 0.32006900721680864 1103 78-79 0.32394344902708383 1103 80-81 0.4503872812106451 1103 82-83 0.45648035530430775 1103 84-85 0.428536966060868 1103 86-87 0.42787969816363614 1103 88-89 0.422238588066044 1103 90-91 0.4508195658108036 1103 92-93 0.44684151401896344 1103 94-95 0.3386851354235816 1103 96-97 0.292185129401922 1103 98-99 0.24700559690763768 1103 100-101 0.18266947650703358 1103 102-103 0.16163151645330842 1103 104-105 0.19704992514610709 1103 106-107 0.016739836383173667 1103 108-109 0.030006117031266655 1103 110-111 0.14337054899523238 1103 112-113 0.12936857403850155 1103 114-115 0.17734912340235454 1103 116-117 0.23045646257946473 1103 118-119 0.2050009651660858 1103 120-121 0.32921184112634805 1103 122-123 0.2509380098699374 1103 124-125 0.3040405089891358 1104 1 0.21528852168270163 1104 2 0.18532548936516946 1104 3 0.22193625676978002 1104 4 0.04379309530479247 1104 5 0.09289270652580939 1104 6 0.15999746094441747 1104 7 0.19019545308032804 1104 8 0.1795819728878172 1104 9 0.2875003544379098 1104 10-11 0.28429976903735366 1104 12-13 0.24762101640516931 1104 14-15 0.36381522032628766 1104 16-17 0.30964249186242654 1104 18-19 0.36376373732461786 1104 20-21 0.36039025095927 1104 22-23 0.2659197685617656 1104 24-25 0.26545764113399883 1104 26-27 0.35900048577074983 1104 28-29 0.39526751898726076 1104 30-31 0.42228920251373125 1104 32-33 0.5153258492578772 1104 34-35 0.4795748095509609 1104 36-37 0.48911008810193124 1104 38-39 0.5338945627908274 1104 40-41 0.48134522917801803 1104 42-43 0.6537513421329422 1104 44-45 0.7101281159840553 1104 46-47 0.733646115466712 1104 48-49 0.9908777369499049 1104 50-51 1.1477263377803268 1104 52-53 1.1205014684494117 1104 54-55 1.2630346429394095 1104 56-57 1.2516826667113605 1104 58-59 1.2278399741041568 1104 60-61 1.2108246478308828 1104 62-63 1.11832312225647 1104 64-65 1.1121908707610046 1104 66-67 1.1315556366914024 1104 68-69 1.0695637220002183 1104 70-71 1.0649827151022038 1104 72-73 1.0408299659796612 1104 74-75 0.9310194838328059 1104 76-77 0.9951459429754479 1104 78-79 1.033928611416485 1104 80-81 1.0895585731818542 1104 82-83 1.1534386451323115 1104 84-85 1.0896170891580823 1104 86-87 1.0844920518483079 1104 88-89 1.1536943225277696 1104 90-91 1.1334829169126337 1104 92-93 1.1957561702809798 1104 94-95 1.1444676819971917 1104 96-97 1.3142382150753846 1104 98-99 1.185228930424156 1104 100-101 1.1846340860580575 1104 102-103 1.1364748972303609 1104 104-105 1.1803617347850555 1104 106-107 1.2677180196001885 1104 108-109 1.144068874628676 1104 110-111 1.0986598824018365 1104 112-113 1.0840163181721465 1104 114-115 1.1498390942766683 1104 116-117 1.172663969311344 1104 118-119 1.1222661737169446 1104 120-121 1.1147341799734072 1104 122-123 1.1472670751004657 1104 124-125 1.1252184504080525 1105 1 0.06252797577699454 1105 2 0.08218239589949405 1105 3 0.06849332873007796 1105 4 5.342036588018573E-4 1105 5 -0.04390423309371272 1105 6 0.0606178083389608 1105 7 0.04747419584293766 1105 8 0.012770558495759587 1105 9 0.0598431997646216 1105 10-11 0.07683492205638487 1105 12-13 0.1181907020958235 1105 14-15 0.09170500527252301 1105 16-17 0.1699609368582884 1105 18-19 0.1002608423701048 1105 20-21 0.17459103673263598 1105 22-23 0.0693006618620089 1105 24-25 0.031312686626137065 1105 26-27 -0.06172945633016269 1105 28-29 -0.05313508647180498 1105 30-31 -0.04370542114218523 1105 32-33 0.0036271313091589263 1105 34-35 0.06123837448065217 1105 36-37 -0.012621715041156278 1105 38-39 -0.07155415515789088 1105 40-41 -0.037983761723552334 1105 42-43 0.027293525755766268 1105 44-45 -0.10217544067433693 1105 46-47 0.05093829908953751 1105 48-49 0.12659423819059867 1105 50-51 0.23390293000530704 1105 52-53 0.14389373478523027 1105 54-55 0.08738327817513891 1105 56-57 0.11925400666173402 1105 58-59 -0.017993359229166117 1105 60-61 -0.078841398488386 1105 62-63 -0.0787819232356739 1105 64-65 -0.023225795905666757 1105 66-67 -0.1327537098760132 1105 68-69 -0.00960811422806529 1105 70-71 -0.12172117240813662 1105 72-73 -0.20582015808982135 1105 74-75 -0.008749953718890424 1105 76-77 0.048495818905969656 1105 78-79 0.1455032598862971 1105 80-81 -0.047641592244119124 1105 82-83 -0.02600200830028143 1105 84-85 0.07785116690828886 1105 86-87 0.08627555060761694 1105 88-89 0.09124084858236614 1105 90-91 0.023702106325373506 1105 92-93 -0.00981144758501884 1105 94-95 0.03299125519818347 1105 96-97 0.03489578331359766 1105 98-99 0.014674753418365327 1105 100-101 0.11060596364283981 1105 102-103 0.17223792452564624 1105 104-105 0.18253812022757288 1105 106-107 0.14423787071681105 1105 108-109 0.20097540895456234 1105 110-111 0.15380359621490314 1105 112-113 0.16752024703898627 1105 114-115 0.24054939204342318 1105 116-117 0.28765052845113104 1105 118-119 0.2772651398046193 1105 120-121 0.28316883671451976 1105 122-123 0.21504002795406763 1105 124-125 0.10504682096222595 1106 1 0.0830685572427754 1106 2 0.11901865675149992 1106 3 0.15964263218253194 1106 4 0.013122533963667138 1106 5 0.09784571643182716 1106 6 0.1516535442565896 1106 7 0.13276593822129712 1106 8 0.17235565843518685 1106 9 0.16077950099619898 1106 10-11 0.1857285218948661 1106 12-13 0.1572793857219139 1106 14-15 0.2931649790258035 1106 16-17 0.2917608560991596 1106 18-19 0.2374302346576087 1106 20-21 0.1961852725493145 1106 22-23 0.26755172182567577 1106 24-25 0.29435654893180896 1106 26-27 0.33556258889495894 1106 28-29 0.24249256343734515 1106 30-31 0.2527184633722541 1106 32-33 0.27753057366732037 1106 34-35 0.3226342456698319 1106 36-37 0.3506417111651814 1106 38-39 0.37661109822389705 1106 40-41 0.4323612312100238 1106 42-43 0.45841860146745006 1106 44-45 0.5256157969594213 1106 46-47 0.5719747921240668 1106 48-49 0.682140119474667 1106 50-51 0.6798574020424581 1106 52-53 0.7576871428207639 1106 54-55 0.7423272515246211 1106 56-57 0.7880872895206039 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-0.5504508530899379 2216 114-115 -0.5724922567174993 2216 116-117 -0.6149914641000791 2216 118-119 -0.6373737013356191 2216 120-121 -0.5885096477432938 2216 122-123 -0.6376941791795794 2216 124-125 -0.906332279453185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 8.0 5 28.0 6 140.0 7 268.0 8 382.0 9 579.0 10 1233.0 11 1929.0 12 2227.0 13 3731.0 14 7218.0 15 7900.0 16 2703.0 17 1858.0 18 1663.0 19 1496.0 20 1468.0 21 1497.0 22 1562.0 23 1573.0 24 1751.0 25 1914.0 26 2073.0 27 2386.0 28 2639.0 29 3103.0 30 3834.0 31 4695.0 32 5948.0 33 7508.0 34 10815.0 35 27003.0 36 36286.0 37 162973.0 38 171486.0 39 647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1594070667308 21.679096852235265 15.32308505302009 19.838411028013848 2 14.669600160158838 22.714073132322984 43.036908769315545 19.57941793820263 3 16.018236266559896 32.094879769838336 31.47634094140908 20.410543022192687 4 10.194532908156411 19.9048518528469 42.600702244578684 27.299912994418 5 9.567847703997359 40.91261975553353 36.02762636273538 13.491906177733728 6 24.376675838409444 43.55373726156393 18.424056524907165 13.645530375119463 7 23.713810439764558 36.719349805585594 24.42583771556884 15.14100203908101 8 24.36681168632825 35.90516209455723 21.422733689415765 18.30529252969876 9 27.751727440086515 15.974383528848456 22.8119092237458 33.461979807319224 10-11 22.036212262554383 29.42870646314382 29.088894025575197 19.446187248726606 12-13 24.502612830525123 28.00764448994081 29.65201472761345 17.837727951920616 14-15 22.261687566559072 27.729437890583082 29.60268878010121 20.40618576275664 16-17 17.742481404736942 31.158107204477727 30.659584919055916 20.439826471729422 18-19 17.51267202166214 27.999698678312267 36.5664187298259 17.921210570199698 20-21 18.705266112454904 26.90720117556735 37.411667340856745 16.975865371121 22-23 18.782969958640503 26.456766529352034 37.553969603658786 17.206293908348673 24-25 18.1822872379946 26.95343135231522 37.54994510166511 17.314336308025062 26-27 17.44879595155738 27.554423650037563 37.56655907143388 17.43022132697118 28-29 17.75190854661518 25.967791534494182 38.34450220935296 17.935797709537677 30-31 17.9887540619218 26.814720641941015 37.74550828069646 17.45101701544072 32-33 17.756533888921567 27.236825675936444 37.49064818283228 17.515992252309704 34-35 17.832332363321157 26.68441582564547 37.89197779291272 17.591274018120654 36-37 18.354835314294913 26.069337439038737 36.92018434397116 18.655642902695195 38-39 17.84947323992789 27.31784235276878 37.15761111288836 17.675073294414968 40-41 17.657244516358052 26.581422898797193 38.13556173792443 17.62577084692033 42-43 17.838755981354993 26.762210116474538 36.51183165420261 18.88720224796786 44-45 17.953038833669055 27.098537133044942 36.470441336723574 18.477982696562428 46-47 18.550309474522784 26.904780086310264 35.89429778486826 18.650612654298694 48-49 18.422231211534573 27.653108398723703 34.883886520021626 19.0407738697201 50-51 18.938026613831145 27.272211300816785 34.08159579792476 19.70816628742731 52-53 18.676269059628673 27.82200143643763 34.26780893728381 19.233920566649882 54-55 19.039609355006483 27.86100814806865 34.273174939942045 18.826207556982823 56-57 19.050537947317693 28.004837707702563 33.68852509767258 19.25609924730717 58-59 18.499871007687943 27.359269387544504 34.50121252773335 19.63964707703421 60-61 19.200271605091153 28.613369327199518 33.53672757142533 18.649631496284005 62-63 18.822699016783208 28.34962396704449 34.94856892836799 17.879108087804312 64-65 18.919585492655724 27.307559743832645 34.63511837317632 19.137736390335316 66-67 18.960042680579818 28.116244728133168 33.849951344551776 19.073761246735234 68-69 18.830800283987717 28.065870290261863 34.1741860119539 18.92914341379652 70-71 19.52297758739718 27.080458963544825 34.73721251572412 18.659350933333883 72-73 18.995443025434852 28.114964543105515 33.58178613590021 19.307806295559427 74-75 18.813450532576866 28.3329652798273 33.62292608728853 19.23065810030731 76-77 18.71923469861614 27.79542429026965 34.71306357904297 18.772277432071245 78-79 18.290996598751775 27.724713535647062 34.30978189082984 19.674507974771323 80-81 18.710012960960267 28.751455012259253 33.122889716262286 19.41564231051819 82-83 19.63977611385832 27.747393361016403 33.40656551063707 19.206265014488206 84-85 18.489078120743315 27.826438707866558 33.443095605656595 20.241387565733532 86-87 18.574005432045766 28.63495083916026 33.603559723609585 19.187484005184384 88-89 18.913921061973202 27.878654041425538 33.72718416946662 19.48024072713464 90-91 18.808684265109697 27.57983743059017 34.12347804012742 19.48800026417271 92-93 19.183514678202293 28.260079912822377 33.29198725357461 19.26441815540072 94-95 18.830697090777004 27.04735330053165 33.894016444870054 20.227933163821284 96-97 19.50310661557941 27.362467378214582 33.30894546370146 19.825480542504547 98-99 18.70598845896659 27.704774916826956 33.514504717954644 20.074731906251806 100-101 18.661241489192644 27.530643034191826 33.76574460664023 20.042370869975297 102-103 19.5213112859419 27.08190583903643 33.36807476080011 20.02870811422156 104-105 18.79793285066827 27.653488314497288 33.458368074760806 20.09021076007364 106-107 19.03448902134229 26.95565402420072 33.7278729327948 20.281984021662183 108-109 19.419643778507005 27.031143582440713 32.62192227519452 20.927290363857757 110-111 19.044872165305904 27.464130336076874 32.63592497502745 20.855072523589772 112-113 18.487239735703458 27.459824593447486 34.031739938476655 20.021195732372405 114-115 18.84203639455712 27.586203338128428 33.036305810044105 20.535454457270347 116-117 18.561634081757745 27.795033940176957 32.854999391161314 20.788332586903984 118-119 18.351870158978475 26.64576673112763 33.27434157979572 21.728021530098175 120-121 18.018624156918428 27.55070872512032 33.14951334483584 21.281153773125407 122-123 18.216087532157417 27.783596698247067 33.079548139900375 20.92076762969514 124-125 18.350998236433487 27.457254924622752 32.84774198475016 21.344004854193603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 617.0 1 483.5 2 902.5 3 1358.0 4 728.5 5 402.0 6 1173.5 7 3028.0 8 5592.5 9 6039.0 10 3575.5 11 1437.5 12 916.0 13 876.0 14 879.5 15 932.0 16 1001.0 17 1082.0 18 1163.0 19 1177.5 20 1221.5 21 1341.5 22 1454.0 23 1607.0 24 1778.5 25 2128.0 26 2950.5 27 3749.0 28 4079.5 29 5140.0 30 7652.5 31 10011.0 32 13735.5 33 18127.0 34 20679.5 35 22958.0 36 27430.5 37 30318.0 38 28152.5 39 26083.0 40 23436.0 41 20463.5 42 17451.0 43 16322.5 44 17228.5 45 16855.0 46 14425.5 47 11237.5 48 9835.5 49 8788.0 50 7950.5 51 6893.5 52 5918.0 53 5624.5 54 5471.5 55 5118.5 56 4712.0 57 4167.0 58 3863.5 59 3445.0 60 2717.5 61 2267.0 62 1932.5 63 1326.5 64 866.0 65 688.0 66 481.0 67 342.0 68 263.0 69 182.0 70 124.5 71 107.5 72 98.5 73 89.5 74 72.0 75 46.0 76 33.5 77 19.0 78 8.5 79 4.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01919377873907193 2 0.0026830013291175812 3 6.19154152873288E-4 4 0.13518199004400122 5 0.04375356013637902 6 0.01506608438658334 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.0638471762442933E-4 30-31 3.09577076436644E-4 32-33 1.0319235881221467E-4 34-35 4.1276943524885866E-4 36-37 6.19154152873288E-4 38-39 0.002786193687929796 40-41 0.0 42-43 0.0011351159469343613 44-45 8.255388704977173E-4 46-47 2.0638471762442933E-4 48-49 0.001238308305746576 50-51 0.0019606548174320787 52-53 0.0 54-55 0.0 56-57 0.0010319235881221468 58-59 0.0014446930233710054 60-61 0.0010319235881221468 62-63 0.0 64-65 0.0010319235881221468 66-67 5.159617940610734E-4 68-69 8.255388704977173E-4 70-71 0.0011351159469343613 72-73 0.0 74-75 2.0638471762442933E-4 76-77 0.0035085401996152988 78-79 8.255388704977173E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 1.0319235881221467E-4 92-93 8.255388704977173E-4 94-95 8.255388704977173E-4 96-97 3.09577076436644E-4 98-99 0.0 100-101 2.0638471762442933E-4 102-103 0.0 104-105 0.0 106-107 2.0638471762442933E-4 108-109 4.1276943524885866E-4 110-111 0.0 112-113 1.0319235881221467E-4 114-115 0.0018574624586198641 116-117 6.19154152873288E-4 118-119 2.0638471762442933E-4 120-121 0.0 122-123 3.09577076436644E-4 124-125 5.159617940610734E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 484532.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.137809482340458 #Duplication Level Percentage of deduplicated Percentage of total 1 68.54753521389298 17.916824159047586 2 13.198044729388341 6.899359573523201 3 5.468061298179553 4.287694333487291 4 3.055427300370548 3.1944870665690877 5 1.825130816569288 2.3852460781918263 6 1.254517527265385 1.9674204071951689 7 0.8836442534204476 1.616756760224804 8 0.6529820759387589 1.365401687701636 9 0.4792581880225225 1.1274083290245955 >10 3.615311351916866 19.062702506487465 >50 0.5763650842691767 10.588162872394076 >100 0.4152986771583383 19.65229232220195 >500 0.0221071539171739 3.853111446595393 >1k 0.004737247267965836 2.6082949801038597 >5k 0.0015790824226552785 3.47483747725206 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9990 2.0617833290680494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6848 1.4133225462920922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4734 0.9770252532340487 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.3586966392312582 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1663 0.343217785409426 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1647 0.3399156299274351 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1468 0.3029727654726623 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.28666837278033236 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 905 0.18677816945010856 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 883 0.18223770566237113 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 877 0.18099939735662454 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 829 0.17109293091065195 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 815 0.1682035448639099 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 800 0.1651077740995435 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 782 0.16139284918230376 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 739 0.1525183063244533 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 732 0.15107361330108227 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 712 0.14694591894859368 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 704 0.14529484120759825 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 683 0.14096076213748523 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 677 0.13972245383173867 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 640 0.1320862192796348 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 636 0.13126068040913705 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 621 0.12816490964477062 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 620 0.12795852492714618 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 618 0.12754575549189734 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 599 0.12362444585703317 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 554 0.11433713356393387 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 553 0.11413074884630943 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 550 0.11351159469343614 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 539 0.11124136279956742 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 537 0.11082859336431856 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.11041582392906969 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 519 0.10711366844707883 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 508 0.10484343655321011 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 504 0.10401789768271238 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 497 0.10257320465934139 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 489 0.10092212691834594 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 485 0.10009658804784824 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.0638471762442936E-4 0.0 20-21 0.0 0.0 0.0 4.127694352488587E-4 0.0 22-23 0.0 0.0 0.0 0.0013415006645587908 0.0 24-25 0.0 0.0 0.0 0.0033021554819908693 0.0 26-27 0.0 0.0 0.0 0.004540463787737446 0.0 28-29 0.0 0.0 0.0 0.0068106956816061685 0.0 30-31 0.0 0.0 0.0 0.014756507310146699 0.0 32-33 0.0 0.0 0.0 0.027036398008800245 0.0 34-35 0.0 0.0 0.0 0.04003863521913929 0.0 36-37 0.0 0.0 0.0 0.06088349169920666 0.0 38-39 0.0 0.0 0.0 0.09617527841298408 0.0 40-41 0.0 0.0 0.0 0.1414767239315463 0.0 42-43 0.0 0.0 0.0 0.18450793755623984 0.0 44-45 0.0 0.0 0.0 0.23032534486886314 0.0 46-47 0.0 0.0 0.0 0.2860492186274591 0.0 48-49 0.0 0.0 0.0 0.3452816325856703 0.0 50-51 0.0 0.0 0.0 0.414833282425103 0.0 52-53 0.0 0.0 0.0 0.4874807030289021 0.0 54-55 0.0 0.0 0.0 0.5649781644968754 0.0 56-57 0.0 0.0 0.0 0.6558906326104365 0.0 58-59 0.0 0.0 0.0 0.7586702219874022 0.0 60-61 0.0 0.0 0.0 0.8744520485747072 0.0 62-63 0.0 0.0 0.0 0.9948775313085617 0.0 64-65 0.0 0.0 0.0 1.1060157017493168 0.0 66-67 0.0 0.0 0.0 1.2370699974408295 0.0 68-69 0.0 0.0 0.0 1.3922713050944004 0.0 70-71 0.0 0.0 0.0 1.5807005522855042 0.0 72-73 0.0 0.0 0.0 1.767994683529674 0.0 74-75 0.0 0.0 0.0 1.947858964939364 0.0 76-77 0.0 0.0 0.0 2.1397967523300836 0.0 78-79 0.0 0.0 0.0 2.3772423699569893 0.0 80-81 0.0 0.0 0.0 2.649670197221236 0.0 82-83 0.0 0.0 0.0 2.91869267664468 0.0 84-85 0.0 0.0 0.0 3.1917396580618 0.0 86-87 0.0 0.0 0.0 3.4678824102432864 0.0 88-89 0.0 0.0 0.0 3.765695557775338 0.0 90-91 0.0 0.0 0.0 4.114485730560624 0.0 92-93 0.0 0.0 0.0 4.495575111654132 0.0 94-95 0.0 0.0 0.0 4.879966648229631 0.0 96-97 0.0 0.0 0.0 5.266525224340188 0.0 98-99 0.0 0.0 0.0 5.692812858593447 0.0 100-101 0.0 0.0 0.0 6.172141365276184 0.0 102-103 0.0 0.0 0.0 6.696668125118672 0.0 104-105 0.0 0.0 0.0 7.235951392271305 0.0 106-107 0.0 0.0 0.0 7.77203569630076 0.0 108-109 0.0 0.0 0.0 8.296975225578496 0.0 110-111 0.0 0.0 0.0 8.899309023965394 0.0 112-113 0.0 0.0 0.0 9.550452808070467 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 35 5.4569682E-11 119.24605 2 GGTATCA 1980 0.0 92.76611 1 ATCACGC 45 3.9835868E-10 92.746925 3 CCGTTGC 30 4.2639163E-5 79.32507 9 TAACGCA 55 1.9572326E-9 75.88385 4 TCACGCA 60 3.8962753E-9 69.56019 4 CACGCAG 60 3.936293E-9 69.47397 5 ATGGGCG 35 9.109783E-5 68.05613 5 GTATCAA 4310 0.0 64.478134 1 TATAACG 65 7.3378033E-9 64.209404 2 TCCGTGC 35 0.007796753 50.994694 8 GTGGTAT 600 0.0 50.690056 1 ATCAACG 5530 0.0 50.350727 3 TCAACGC 5620 0.0 49.5444 4 CAACGCA 5660 0.0 49.23849 5 GTATGGT 75 1.4462148E-6 47.70829 1 TATCAAC 5850 0.0 47.69842 2 CATATAG 125 1.0913936E-11 47.698418 3 AACGCAG 5840 0.0 47.579586 6 GTATCAC 90 9.536416E-8 46.383053 1 >>END_MODULE