FastQCFastQC Report
Fri 27 May 2016
SRR940080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962404
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.061.741491
GTATCAA16400.051.2933161
CAACGCA20050.041.8857655
TCAACGC20150.041.4038544
ATCAACG20800.040.3943823
AACGCAG21350.039.3353466
TATCAAC23400.035.9360542
GTACATG46500.035.282981
TACATGG50800.031.6976572
ACATGGG49250.031.4580843
ACGCAGA26000.031.3840487
CGCAGAG27000.030.001088
CATGGGG27500.027.7373564
GCAGAGT31550.025.4790449
ATGGGGA17650.024.9717375
GTATTAG2752.050001E-923.8641281
TGGTATC5000.023.8554232
GAGTACA33600.023.79311
CATGGGA24850.023.7408684
GTGTTAA3800.023.5501231
Produced by FastQC (version 0.11.5)