FastQCFastQC Report
Fri 27 May 2016
SRR940080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962404
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.39629926725159076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.2927045191000869No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.17851131125805794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.080.409371
GTATCAA37300.059.9394451
ATCAACG46700.047.3540233
TCAACGC46800.047.2503784
CAACGCA47200.046.789075
TATCAAC48800.045.8048362
AACGCAG49150.045.1561856
ACGCAGA56150.039.4166767
GTGGTAT7400.038.672281
CGCAGAG57350.038.488178
GCAGAGT64750.034.0895279
GTACATG45600.033.993731
TGGTATC7350.032.4393772
TACATGG49900.030.699582
ACATGGG47550.030.587163
TATAACG1004.3914927E-429.8036752
GTATAAC2302.6011548E-1028.5138021
CAGAGTA62250.028.43376710-11
GAGTACT43050.028.05496412-13
GTACTTT43100.027.81535514-15
Produced by FastQC (version 0.11.5)