FastQCFastQC Report
Fri 27 May 2016
SRR940079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100588
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.048.496391
GTATCAA19150.046.115391
TCAACGC21300.040.866734
CAACGCA21300.040.8351025
AACGCAG22050.039.1759766
ATCAACG22650.038.423963
GTACATG55550.037.1659281
TACATGG54600.036.497842
TATCAAC25350.036.0103072
ACATGGG56250.034.9710733
CATGGGG36200.031.78674
ACGCAGA27550.030.9225187
GTGGTAT6750.029.1717931
CGCAGAG29750.028.0350598
AGTACAT40700.025.9473042
ATGGGGA19550.025.9019575
GAGTACA44000.025.6307961
ATGGGGG18800.023.7664225
GCAGAGT35050.023.2731829
TAAGGTG5900.023.2239595
Produced by FastQC (version 0.11.5)