##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100588 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73347519689475 34.0 31.0 34.0 30.0 34.0 2 31.72806717863542 34.0 31.0 34.0 30.0 34.0 3 31.91052510112776 34.0 31.0 34.0 30.0 34.0 4 35.08088312792798 37.0 37.0 37.0 33.0 37.0 5 35.11228725008814 37.0 37.0 37.0 33.0 37.0 6 35.10378361385005 37.0 37.0 37.0 33.0 37.0 7 35.02532373603928 37.0 37.0 37.0 33.0 37.0 8 34.99546878577633 37.0 37.0 37.0 33.0 37.0 9 36.636117239148525 39.0 39.0 39.0 33.0 39.0 10-11 36.67378892010453 39.0 39.0 39.0 33.5 39.0 12-13 36.648250753233725 39.0 39.0 39.0 33.0 39.0 14-15 38.17141246315606 41.0 40.0 41.0 33.5 41.0 16-17 38.22980079739194 41.0 40.0 41.0 34.0 41.0 18-19 38.24967108491097 41.0 40.0 41.0 34.0 41.0 20-21 38.28311048276012 41.0 40.0 41.0 34.0 41.0 22-23 38.317441222328426 41.0 40.0 41.0 34.0 41.0 24-25 38.381578756083115 41.0 39.5 41.0 33.5 41.0 26-27 38.430556666073045 41.0 39.5 41.0 33.5 41.0 28-29 38.388719938796356 41.0 39.0 41.0 33.0 41.0 30-31 38.33321824333902 41.0 39.0 41.0 33.0 41.0 32-33 38.292598138449634 41.0 39.0 41.0 33.0 41.0 34-35 38.21453168669838 41.0 39.0 41.0 33.0 41.0 36-37 38.074379786077984 41.0 39.0 41.0 33.0 41.0 38-39 37.97585063620538 41.0 39.0 41.0 32.0 41.0 40-41 37.87711795876386 41.0 38.0 41.0 32.0 41.0 42-43 37.71060514924749 40.5 38.0 41.0 32.0 41.0 44-45 37.55248921485605 40.0 38.0 41.0 31.0 41.0 46-47 37.37855173779834 40.0 38.0 41.0 31.0 41.0 48-49 37.217370169400354 40.0 37.0 41.0 30.5 41.0 50-51 37.04094038822884 40.0 37.0 41.0 30.0 41.0 52-53 37.155348777199094 40.0 37.0 41.0 31.0 41.0 54-55 36.94064808993011 40.0 36.0 41.0 30.0 41.0 56-57 36.81077478584175 40.0 36.0 41.0 30.0 41.0 58-59 36.60873006065849 39.5 35.0 41.0 30.5 41.0 60-61 36.359712262899464 39.0 35.0 41.0 31.0 41.0 62-63 36.064151617135565 39.0 35.0 41.0 30.0 41.0 64-65 35.750858177628686 38.0 35.0 41.0 30.0 41.0 66-67 35.420040014973814 37.0 35.0 40.0 30.0 41.0 68-69 35.071178315591304 37.0 35.0 40.0 30.0 41.0 70-71 34.70333358168543 36.0 35.0 39.0 29.5 41.0 72-73 34.33570782163716 36.0 35.0 39.0 29.0 41.0 74-75 33.97020774349711 35.0 34.0 38.0 29.0 40.0 76-77 33.61528246719027 35.0 34.0 37.0 29.0 39.0 78-79 33.27837982969103 35.0 34.0 37.0 28.0 39.0 80-81 32.971878668493574 35.0 34.0 36.0 28.0 38.5 82-83 32.688907656634456 35.0 34.0 36.0 27.0 37.0 84-85 32.468020276434046 35.0 34.0 35.5 27.0 37.0 86-87 32.270705750017264 35.0 34.0 35.0 27.0 36.5 88-89 32.11583853358387 35.0 34.0 35.0 27.0 36.0 90-91 31.982706516880068 35.0 34.0 35.0 26.5 36.0 92-93 31.845716108116754 35.0 33.0 35.0 26.0 36.0 94-95 31.748777471678775 35.0 33.0 35.0 26.0 35.5 96-97 31.66124108203978 35.0 33.0 35.0 25.0 35.0 98-99 31.590755123624827 35.0 33.0 35.0 25.0 35.0 100-101 31.50037616256038 35.0 33.0 35.0 25.0 35.0 102-103 30.804317328555282 34.0 32.0 35.0 24.5 35.0 104-105 31.726849647642894 35.0 33.5 35.0 26.5 35.0 106-107 31.84307388414193 35.0 34.0 35.0 27.0 35.0 108-109 31.828981871508685 35.0 34.0 35.0 27.0 35.0 110-111 31.786267431591114 35.0 34.0 35.0 25.5 35.0 112-113 31.742502644041185 35.0 34.0 35.0 25.0 35.0 114-115 31.686344481313625 35.0 34.0 35.0 25.0 35.0 116-117 31.61494946337776 35.0 34.0 35.0 25.0 35.0 118-119 31.52842026262325 35.0 34.0 35.0 25.0 35.0 120-121 31.45461925806932 35.0 34.0 35.0 25.0 35.0 122-123 31.326843014824803 35.0 34.0 35.0 24.5 35.0 124-125 30.074594216909507 34.5 31.5 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05127602338462012 1101 2 0.16003507703354458 1101 3 0.2407028525950956 1101 4 0.1805354587419572 1101 5 0.11980087871847189 1101 6 0.23556382555631217 1101 7 0.24920686518638746 1101 8 0.18354874608582605 1101 9 0.29578638795013745 1101 10-11 0.12911261539647967 1101 12-13 0.12028977654610173 1101 14-15 0.14914985964301763 1101 16-17 0.0914169295927536 1101 18-19 0.18950025537047566 1101 20-21 -0.019514592998909563 1101 22-23 0.12440730101775443 1101 24-25 0.12055579212460543 1101 26-27 0.1348478829844737 1101 28-29 0.09642314511664551 1101 30-31 0.1907093503558741 1101 32-33 0.247052870450581 1101 34-35 0.2491838563517348 1101 36-37 0.2175946933150925 1101 38-39 0.21354377504992783 1101 40-41 0.25342912881647095 1101 42-43 0.2616217858518084 1101 44-45 0.2145227114784376 1101 46-47 0.28876480142587013 1101 48-49 0.17354133638409763 1101 50-51 0.24582417217658303 1101 52-53 0.09398760480920743 1101 54-55 0.22617604730356078 1101 56-57 0.1360047338522179 1101 58-59 0.15268097376255696 1101 60-61 0.06817459814951832 1101 62-63 0.1304340018212926 1101 64-65 0.13134898282382323 1101 66-67 0.24174757749842968 1101 68-69 0.1499804676125791 1101 70-71 0.16902166603117763 1101 72-73 0.19018721541042538 1101 74-75 0.08250023414445451 1101 76-77 0.07289621327898743 1101 78-79 0.1579145296083766 1101 80-81 0.04671132408265066 1101 82-83 0.011065142179745635 1101 84-85 0.05232069660635119 1101 86-87 -0.08096543452942484 1101 88-89 -0.06116946691649616 1101 90-91 -0.0011924933056093323 1101 92-93 0.009876689610784695 1101 94-95 -0.05867859150784582 1101 96-97 0.017126046255118155 1101 98-99 -0.010744866323985747 1101 100-101 -0.08653933216312737 1101 102-103 -0.037648653092592355 1101 104-105 0.03391869111552026 1101 106-107 0.03881919732501515 1101 108-109 0.02006710295571068 1101 110-111 0.048715605028711195 1101 112-113 0.11523775508106482 1101 114-115 0.09689715723871828 1101 116-117 0.09553221304735615 1101 118-119 0.010758386486802607 1101 120-121 0.05516109313329309 1101 122-123 0.09780251342660051 1101 124-125 -0.045405012698338965 1102 1 0.16412763221254778 1102 2 0.15472979076186633 1102 3 0.35099877122552314 1102 4 0.164630879770165 1102 5 0.16049801600168934 1102 6 0.24299724225305397 1102 7 0.23555664345689564 1102 8 0.2564842124912303 1102 9 0.2788634002741688 1102 10-11 0.2864046580579256 1102 12-13 0.260006212029694 1102 14-15 0.37533327633976654 1102 16-17 0.38306291654681246 1102 18-19 0.39321100151599353 1102 20-21 0.30735082450568996 1102 22-23 0.2738520641625257 1102 24-25 0.35162015813295966 1102 26-27 0.3986557539516653 1102 28-29 0.4450914253380276 1102 30-31 0.5073863837356214 1102 32-33 0.41015215240799563 1102 34-35 0.36998184617624474 1102 36-37 0.44025665037863604 1102 38-39 0.44217840911032624 1102 40-41 0.3810124814866853 1102 42-43 0.4107257365900452 1102 44-45 0.3642210025284811 1102 46-47 0.44365450604437484 1102 48-49 0.5417567792414886 1102 50-51 0.46718706729338066 1102 52-53 0.379552144849427 1102 54-55 0.53482666651324 1102 56-57 0.4065429112873744 1102 58-59 0.3521241792146981 1102 60-61 0.31353106268138475 1102 62-63 0.35438078674368256 1102 64-65 0.35865528105857436 1102 66-67 0.30212965264581726 1102 68-69 0.27211404459052346 1102 70-71 0.2596208298187932 1102 72-73 0.22260226207674805 1102 74-75 0.2675551938378149 1102 76-77 0.2533073689365253 1102 78-79 0.3110238989684575 1102 80-81 0.1540685749807409 1102 82-83 0.20545632069776332 1102 84-85 0.09156058556972368 1102 86-87 0.10185343052497586 1102 88-89 0.12484696512943572 1102 90-91 0.18336749612356584 1102 92-93 0.06060483034885067 1102 94-95 0.11883794151953353 1102 96-97 0.1497512202869835 1102 98-99 0.09692311739866 1102 100-101 0.27010890001686505 1102 102-103 0.15972040404142618 1102 104-105 0.27935169744082344 1102 106-107 0.23758588075030218 1102 108-109 0.2433228631956581 1102 110-111 0.1502489654336685 1102 112-113 0.279689006821382 1102 114-115 0.257478079908406 1102 116-117 0.24131693562766898 1102 118-119 0.30321303077425554 1102 120-121 0.2939056895512451 1102 122-123 0.391580122185772 1102 124-125 0.18042389463936104 1103 1 0.053260768783289336 1103 2 0.1989849653075062 1103 3 0.1943221464520697 1103 4 0.14485687445164785 1103 5 0.18956196102108436 1103 6 0.20306018793303338 1103 7 0.21286454586039127 1103 8 0.16312960790781972 1103 9 0.12143885886933958 1103 10-11 0.1549581777652307 1103 12-13 0.19872786212539495 1103 14-15 0.46697397147348596 1103 16-17 0.2614380689884186 1103 18-19 0.2920247094040036 1103 20-21 0.22790731047259527 1103 22-23 0.1911358193921231 1103 24-25 0.28599457589071875 1103 26-27 0.40024553994058465 1103 28-29 0.4230278847123472 1103 30-31 0.4594940477373797 1103 32-33 0.52947132066965 1103 34-35 0.46573158363791833 1103 36-37 0.4994166971273941 1103 38-39 0.45525583462566743 1103 40-41 0.6027623523347714 1103 42-43 0.601589508842288 1103 44-45 0.5524908855718849 1103 46-47 0.5315389447205021 1103 48-49 0.6495155276958968 1103 50-51 0.6129956112219617 1103 52-53 0.4941027491110219 1103 54-55 0.48470434271033724 1103 56-57 0.49241914352369065 1103 58-59 0.4247027925042204 1103 60-61 0.4407864574204652 1103 62-63 0.4945847197371265 1103 64-65 0.529508181260141 1103 66-67 0.4346040551594115 1103 68-69 0.3710012737949526 1103 70-71 0.4687394844369308 1103 72-73 0.49784616864698705 1103 74-75 0.5664836563795248 1103 76-77 0.5018811400877965 1103 78-79 0.41367214843067046 1103 80-81 0.37406404407744276 1103 82-83 0.3816166236331071 1103 84-85 0.4192221736725017 1103 86-87 0.3737568545328287 1103 88-89 0.33379782811830694 1103 90-91 0.31897568520197694 1103 92-93 0.31036240584979424 1103 94-95 0.3175130228217249 1103 96-97 0.3836305204106303 1103 98-99 0.2420504378986692 1103 100-101 0.13898900469343545 1103 102-103 0.10841665778895049 1103 104-105 0.21066008045882967 1103 106-107 0.2448440596659367 1103 108-109 0.18902427455086723 1103 110-111 0.12513341681326295 1103 112-113 0.08359325867185774 1103 114-115 0.18867292568831573 1103 116-117 0.14319032652182173 1103 118-119 0.17112925987705196 1103 120-121 0.18094884111208032 1103 122-123 0.049519154012191535 1103 124-125 0.10133210785435409 1104 1 -0.17896018864991703 1104 2 -0.024050828566739568 1104 3 -0.07197512590014199 1104 4 -0.11311349635585088 1104 5 -0.1666871751521768 1104 6 0.029739920060038116 1104 7 -0.11321290167458642 1104 8 -0.17103248144771754 1104 9 -0.10245364407219881 1104 10-11 -0.1220870404264005 1104 12-13 -0.20239833500258442 1104 14-15 -0.11921846660663249 1104 16-17 -0.13027079901161187 1104 18-19 -0.06123293329500257 1104 20-21 -0.09910397582818575 1104 22-23 -0.03670194527533255 1104 24-25 0.06820903682918811 1104 26-27 0.013636549723635483 1104 28-29 0.046036812604356214 1104 30-31 -0.006723661966105965 1104 32-33 -0.09448697545766294 1104 34-35 -0.04849625749146469 1104 36-37 -0.05821031710950564 1104 38-39 -0.10759995199114059 1104 40-41 -0.19393178596344285 1104 42-43 -0.1640751964523801 1104 44-45 -0.16096995505900225 1104 46-47 -0.20314136303217367 1104 48-49 -0.23567406900895804 1104 50-51 -0.2506639289711501 1104 52-53 -0.30265997767747166 1104 54-55 -0.28278611054829383 1104 56-57 -0.3264943009074628 1104 58-59 -0.24457411916053218 1104 60-61 -0.2418755628289304 1104 62-63 -0.23161779179221043 1104 64-65 -0.17972709072040516 1104 66-67 -0.16316482595783555 1104 68-69 -0.19103145093847473 1104 70-71 -0.1932854237968442 1104 72-73 -0.14394746437405104 1104 74-75 -0.1376312906831032 1104 76-77 -0.1815529536201268 1104 78-79 -0.1772531543033935 1104 80-81 -0.12505181145915145 1104 82-83 -0.14183365178202934 1104 84-85 -0.07469389782724534 1104 86-87 -0.12428384406553761 1104 88-89 -0.08531071821464309 1104 90-91 -0.11558804799967248 1104 92-93 -0.1871875615124381 1104 94-95 -0.14287906522415383 1104 96-97 -0.037551468924764464 1104 98-99 -0.03303522006683579 1104 100-101 -0.12722440852348882 1104 102-103 -0.20461474124542534 1104 104-105 -0.10048215861113974 1104 106-107 -0.08104237539421888 1104 108-109 -0.0030379924100323308 1104 110-111 -0.11746174389345043 1104 112-113 -0.13942415866348412 1104 114-115 -0.14608653059389454 1104 116-117 -0.16639145968811064 1104 118-119 -0.11354092783799885 1104 120-121 -0.10226054117221395 1104 122-123 -0.09369801371281028 1104 124-125 -0.1386038900253581 1105 1 -0.016913280334350134 1105 2 0.1529214528831524 1105 3 0.2165820880302043 1105 4 0.17828664579056408 1105 5 -0.03164808488920556 1105 6 0.21026586178000883 1105 7 0.09076719782790121 1105 8 0.161590119831601 1105 9 0.1185840247266583 1105 10-11 0.022902298592789805 1105 12-13 0.13591012270886438 1105 14-15 0.22015608918582785 1105 16-17 0.19737667788960067 1105 18-19 0.14061497004544066 1105 20-21 0.19123006823719635 1105 22-23 0.18646791968557608 1105 24-25 0.16771152439137182 1105 26-27 0.1966500536326663 1105 28-29 0.26920667756526484 1105 30-31 0.1681164233217416 1105 32-33 0.2214334225522876 1105 34-35 0.1924419088198377 1105 36-37 0.32594035808595123 1105 38-39 0.28721170401454543 1105 40-41 0.22788768809483884 1105 42-43 0.20550547163575317 1105 44-45 0.11052968957497455 1105 46-47 0.1095807407347138 1105 48-49 0.14847660618649172 1105 50-51 -0.007238200470794709 1105 52-53 0.01148359019744305 1105 54-55 0.13263677502384752 1105 56-57 0.13974592652679263 1105 58-59 0.15734485738531134 1105 60-61 0.12628364115116852 1105 62-63 0.16959045269961592 1105 64-65 0.012170563863818984 1105 66-67 0.05041015627385548 1105 68-69 0.13341773171966054 1105 70-71 0.06968543618894074 1105 72-73 -0.023123015191394813 1105 74-75 -0.0924998052531052 1105 76-77 0.030600564503544092 1105 78-79 0.0800318492502683 1105 80-81 0.029532410289249356 1105 82-83 0.11829427998769404 1105 84-85 0.058923728327698655 1105 86-87 0.15005872878450077 1105 88-89 0.0077951808614074025 1105 90-91 0.17403526401169955 1105 92-93 0.2790526659218173 1105 94-95 0.15953742375949886 1105 96-97 0.18632912809016133 1105 98-99 0.14287096330203397 1105 100-101 0.07817715508703316 1105 102-103 0.0946034534951572 1105 104-105 0.08217597927087894 1105 106-107 0.1382184632696024 1105 108-109 0.08829107653097523 1105 110-111 0.05915517152943295 1105 112-113 0.15801720594205548 1105 114-115 0.10693408063567134 1105 116-117 0.035811809679337614 1105 118-119 0.0602330593688265 1105 120-121 0.03633220936083603 1105 122-123 0.10601769346558498 1105 124-125 0.04298814977563836 1106 1 0.0029694612072717064 1106 2 0.10139806457042866 1106 3 0.06915870134511337 1106 4 0.08522562986296123 1106 5 0.04210528566261473 1106 6 0.10123976198257623 1106 7 0.004271945330593496 1106 8 -0.04043189066710795 1106 9 0.15333788504734258 1106 10-11 0.09867417777129361 1106 12-13 0.23046338001704214 1106 14-15 0.25493683007344003 1106 16-17 0.18248681669690114 1106 18-19 0.15006287279401676 1106 20-21 0.1500379422210969 1106 22-23 0.19416463981251297 1106 24-25 0.21462156623134376 1106 26-27 0.25873429880908105 1106 28-29 0.2454755759601781 1106 30-31 0.26765738215402735 1106 32-33 0.29607878402686083 1106 34-35 0.3019306503012871 1106 36-37 0.26890842643854995 1106 38-39 0.23466167533371873 1106 40-41 0.17951647782320634 1106 42-43 0.24344899482480997 1106 44-45 0.2668012217722193 1106 46-47 0.2640924589356217 1106 48-49 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-0.6667525864712616 2216 20-21 -0.6479359948980914 2216 22-23 -0.6780726283377945 2216 24-25 -0.7334929216512336 2216 26-27 -0.7297676542311393 2216 28-29 -0.7055779503639741 2216 30-31 -0.8618925916644073 2216 32-33 -0.8904549638158414 2216 34-35 -0.9655351721816743 2216 36-37 -0.9991410553423279 2216 38-39 -0.8808093208239356 2216 40-41 -0.9042156229711438 2216 42-43 -0.8854412200837984 2216 44-45 -0.8807950744569197 2216 46-47 -0.8371101347149832 2216 48-49 -0.8666855369491984 2216 50-51 -0.7577931961067961 2216 52-53 -0.8833373509063378 2216 54-55 -0.7481446757136396 2216 56-57 -0.7858926771970403 2216 58-59 -0.7769997919502956 2216 60-61 -0.7571875336838545 2216 62-63 -0.7517158086640805 2216 64-65 -0.8009761221158485 2216 66-67 -0.9208127924369478 2216 68-69 -0.8045540145709467 2216 70-71 -1.068156909598244 2216 72-73 -0.9237247739577938 2216 74-75 -0.8951653036856158 2216 76-77 -0.8823558613752098 2216 78-79 -0.8519417236723612 2216 80-81 -0.9560984783621649 2216 82-83 -0.9027925998310593 2216 84-85 -0.82052235648408 2216 86-87 -0.7793040433743847 2216 88-89 -0.7573732930229937 2216 90-91 -0.7713105383642755 2216 92-93 -0.8632832452923864 2216 94-95 -0.8395969518683017 2216 96-97 -0.8923734735943256 2216 98-99 -0.8044074056269892 2216 100-101 -0.9322850402140332 2216 102-103 -0.9249919019299604 2216 104-105 -0.7715022701336096 2216 106-107 -0.796631801862155 2216 108-109 -0.8571315389292167 2216 110-111 -0.7833488843702341 2216 112-113 -0.7893421965703205 2216 114-115 -0.809901405996321 2216 116-117 -0.7258895241672079 2216 118-119 -0.8321564521843676 2216 120-121 -0.8327483295800207 2216 122-123 -0.9385642829772749 2216 124-125 -0.9174282109048413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16669.0 3 2793.0 4 1795.0 5 1369.0 6 1547.0 7 1936.0 8 1954.0 9 2199.0 10 2572.0 11 2877.0 12 3181.0 13 3415.0 14 3459.0 15 3428.0 16 3152.0 17 3088.0 18 2512.0 19 2132.0 20 2214.0 21 2000.0 22 2043.0 23 2977.0 24 5296.0 25 9208.0 26 11275.0 27 8569.0 28 8599.0 29 10477.0 30 11347.0 31 13667.0 32 18118.0 33 24755.0 34 38034.0 35 70268.0 36 180265.0 37 414848.0 38 205629.0 39 921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28118315395601 18.194760224689073 13.204164693197965 24.31989192815695 2 15.302198202397774 19.62148746485644 40.29373097149184 24.782583361253952 3 19.26340202625332 24.40555602005169 28.386994754424933 27.944047199270056 4 12.882608628425436 16.33758057371853 35.87091469531833 34.9088961025377 5 14.282676321679885 38.18537734434427 32.725441923150676 14.806504410825175 6 32.70104400908214 35.70554690042193 17.181611769689013 14.41179732080692 7 29.546572136251054 32.575090326538955 19.866112209421228 18.012225327788762 8 26.264301065684627 36.37216104689394 18.989141240276094 18.374396647145335 9 26.65619303027105 17.642649472194226 18.889934028826456 36.81122346870826 10-11 25.667576888604472 27.14190008526513 26.031635363394894 21.158887662735502 12-13 25.70548386261191 24.108763828038125 26.79168611290741 23.39406619644255 14-15 22.736754295133718 25.416995884904548 24.154115481655182 27.692134338306555 16-17 23.36736168483966 28.83398363476236 24.78843617730007 23.010218503097907 18-19 23.442901934617016 27.53067923866216 26.185147239436695 22.841271587284126 20-21 24.14024448248537 26.815742388995425 25.853132997944062 23.19088013057515 22-23 24.043328655070763 26.491912487686587 25.877401626871116 23.587357230371538 24-25 23.82377558410572 26.79478022639993 25.757845095312287 23.62359909418206 26-27 23.429835354671734 27.078058860841164 25.691277741529188 23.800828042957907 28-29 23.448193142213807 27.047733539418573 26.076506873369798 23.427566444997822 30-31 23.47513145203097 26.73634127868648 25.871776966123928 23.916750303158622 32-33 23.28481950657467 26.86397500797357 25.861758764791993 23.989446720659764 34-35 23.879369605505936 27.029615565130115 25.382978694403512 23.708036134960434 36-37 24.084071723200456 26.56440745036625 25.561850969958744 23.789669856474546 38-39 23.52405054089296 26.58767191127629 25.789440206452554 24.0988373413782 40-41 23.658572045160263 26.510756229981148 25.949024329297384 23.88164739556121 42-43 23.818448563591662 26.439963633736664 25.740944441491166 24.00064336118051 44-45 23.42977818184627 26.744578583673302 25.873317258434664 23.952325976045767 46-47 23.716973553397562 27.069917830328077 25.569926009127542 23.64318260714682 48-49 23.796612849579333 26.737877523868725 25.54798553972486 23.917524086827083 50-51 23.647276378621765 26.825523755866836 25.69509252906726 23.83210733644414 52-53 23.99585802795604 26.51266491100849 25.709169588220004 23.78230747281547 54-55 23.601925968266144 26.631641075994146 25.83381912673505 23.93261382900466 56-57 23.57163109224148 27.01173919681284 25.77052678259858 23.646102928347098 58-59 23.677160445584235 26.340787605938942 25.790214086176093 24.19183786230073 60-61 23.503698386158767 26.57899280314045 26.182256833381796 23.73505197731899 62-63 23.296197575921706 26.80299570056444 26.067156983470213 23.83364974004364 64-65 23.292412925601628 26.713721954509182 25.78612808175478 24.207737038134415 66-67 23.312199568660347 26.999990002662926 25.691793004227055 23.99601742444967 68-69 23.285621837007735 26.645210429876098 26.152492583328485 23.91667514978768 70-71 23.336356679747638 26.689226647375413 25.99997637063925 23.974440302237703 72-73 23.319768741582713 26.682730317862184 25.939646351282335 24.057854589272765 74-75 23.341132960162838 27.04083672579238 25.71232553067752 23.905704783367256 76-77 23.607969144078957 26.794336417320064 25.745920264032762 23.85177417456822 78-79 23.65685406237092 26.802255491842725 25.900940303261777 23.639950142524583 80-81 23.555881302499053 27.139698485155545 25.837462978597337 23.46695723374807 82-83 23.65290520989342 26.955709362685937 25.875707036191532 23.515678391229116 84-85 23.58439426992008 26.936800909848067 25.730425248556553 23.748379571675297 86-87 23.542961372844477 26.631125296960683 26.084372279185757 23.741541051009076 88-89 23.444586881057752 26.91724806404104 25.811453206391562 23.82671184850965 90-91 23.401181680946145 27.240826794947182 25.905550955787394 23.45244056831928 92-93 23.467855620460647 26.678067219940395 26.065972938606976 23.788104220991986 94-95 23.30505181965207 26.762270331039577 26.107816322088436 23.824861527219916 96-97 23.612006698158005 26.72555956202953 25.99921385255418 23.663219887258276 98-99 23.53315691816054 26.814288265664345 25.740755131468383 23.91179968470673 100-101 23.12857351228518 26.829872302101883 26.10293532950947 23.938618856103467 102-103 23.203704107636884 26.373408149836592 26.335372791949773 24.087514950576754 104-105 23.15696582511266 26.635800042805513 26.367924764081174 23.83930936800065 106-107 23.37497438050819 26.827732037611913 25.886025413626978 23.911268168252917 108-109 23.532696835124668 26.693482535226497 25.746778165182054 24.027042464466778 110-111 23.235487504697357 27.020589292441024 26.113017316707765 23.630905886153858 112-113 23.274142734515692 27.003618507600773 26.07420326260968 23.648035495273856 114-115 23.015133051445833 26.96891876787355 26.049649276334264 23.966298904346356 116-117 23.061467987448875 27.01786254644653 26.146998661467624 23.773670804636975 118-119 23.069351519147773 27.237550004703387 25.746281713195334 23.946816762953507 120-121 23.01270193107704 27.220475042134527 26.09414596973948 23.67267705704895 122-123 23.08380172574394 27.100677483994385 26.02065621890321 23.794864571358467 124-125 23.218677014513037 27.206944810476287 25.792537191359425 23.78184098365125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 472.0 1 485.5 2 527.0 3 477.0 4 359.0 5 280.5 6 247.5 7 240.5 8 232.0 9 247.0 10 244.5 11 235.0 12 247.0 13 254.0 14 255.5 15 280.5 16 320.5 17 365.5 18 479.0 19 590.0 20 761.0 21 1085.0 22 1423.5 23 1877.5 24 2447.5 25 3109.5 26 3914.5 27 4748.5 28 5721.5 29 7217.0 30 9001.0 31 11104.5 32 13431.5 33 14840.0 34 15830.5 35 16645.0 36 18705.5 37 21387.0 38 23283.0 39 24673.0 40 26170.5 41 29957.0 42 33475.5 43 35911.0 44 38743.5 45 41853.5 46 43456.5 47 43675.5 48 44511.5 49 45065.5 50 44765.0 51 44007.0 52 43828.0 53 44246.5 54 43325.0 55 40812.0 56 36493.0 57 32444.5 58 30643.0 59 27923.5 60 24242.0 61 20638.0 62 16875.0 63 12890.5 64 10053.5 65 8345.0 66 6452.0 67 5359.0 68 4127.0 69 2705.5 70 2144.5 71 1697.5 72 1402.0 73 1222.0 74 949.0 75 774.5 76 550.5 77 322.5 78 199.0 79 130.5 80 85.5 81 60.5 82 44.5 83 35.5 84 28.5 85 24.5 86 17.5 87 12.5 88 10.5 89 7.5 90 8.0 91 6.5 92 4.0 93 3.5 94 3.5 95 4.0 96 5.0 97 4.5 98 4.0 99 5.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05260824213965626 2 0.2031641268122131 3 0.021079641064594562 4 0.1025815291462382 5 0.020625338455443818 6 0.036889371863040483 7 0.037525395515851524 8 0.10058259766597491 9 0.11993588881579664 10-11 0.044521655696773 12-13 0.030211123508524534 14-15 0.011584716533343996 16-17 0.029893111682119014 18-19 0.05242652109599596 20-21 0.1874906867965124 22-23 0.10694283419408536 24-25 0.07346073189967545 26-27 0.014719404536484135 28-29 0.006587387832685801 30-31 0.008313737747458631 32-33 0.00699626018092147 34-35 0.009313203487590271 36-37 0.004679316874252672 38-39 0.008450028530203855 40-41 0.005724212875299385 42-43 0.010676111315042505 44-45 0.010403529749552057 46-47 0.016536614973087112 48-49 0.0176269412350489 50-51 0.011175844185108324 52-53 0.01326563618720175 54-55 0.014037950622758016 56-57 0.01562800975478562 58-59 0.017263499147728303 60-61 0.009812936357656088 62-63 0.019171570106161436 64-65 0.029257088029307973 66-67 0.026803853939893946 68-69 0.033073229946174225 70-71 0.023805456719499032 72-73 0.01580973079844592 74-75 0.009812936357656088 76-77 0.010766971836872655 78-79 0.022851421240282464 80-81 0.019171570106161436 82-83 0.019989314802632774 84-85 0.016763766277662486 86-87 0.02489578298146082 88-89 0.026667563157148723 90-91 0.026440411852573353 92-93 0.03171032211872199 94-95 0.025577236895186935 96-97 0.02594067898250753 98-99 0.031119728726826022 100-101 0.0197621634980574 102-103 0.0268947144617241 104-105 0.023850886980414106 106-107 0.03193747342329736 108-109 0.022215397587471424 110-111 0.021306792369169936 112-113 0.024759492198715597 114-115 0.013538217752692198 116-117 0.011721007316089218 118-119 0.02875735515924215 120-121 0.022124537065641273 122-123 0.017536080713218754 124-125 0.017990383322369494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1100588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.858604981664584 #Duplication Level Percentage of deduplicated Percentage of total 1 69.93420201761163 32.07084945033582 2 14.469065522679102 13.270623205167265 3 5.795346998283561 7.972995861778842 4 3.0361578137788503 5.569358473763145 5 1.708433746051867 3.9173194148769017 6 1.1263022753193372 3.099039068029171 7 0.7358572893425892 2.362177212838818 8 0.5386772553270824 1.976238997172156 9 0.4072903946135198 1.6809992387466925 >10 2.0315290213740056 16.788262007719524 >50 0.14371441245734445 4.513461492123321 >100 0.06945442930506358 5.426380467310398 >500 0.0037703826632386754 1.2591996930671825 >1k 1.9844119280203556E-4 0.09309541707073266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.543026091507449E-5 0.0 24-25 0.0 0.0 0.0 1.8172104366029796E-4 0.0 26-27 0.0 0.0 0.0 4.543026091507449E-4 0.0 28-29 0.0 0.0 0.0 9.540354792165642E-4 0.0 30-31 0.0 0.0 0.0 0.0033164090468004374 0.0 32-33 0.0 0.0 0.0 0.006950829920006397 0.0 34-35 0.0 0.0 0.0 0.01194815862066459 0.0 36-37 0.0 0.0 0.0 0.020080175324462924 0.0 38-39 0.0 0.0 0.0 0.0354356035137581 0.0 40-41 0.0 0.0 0.0 0.05383485918436327 0.0 42-43 0.0 0.0 0.0 0.07164352146307247 0.0 44-45 0.0 0.0 0.0 0.09226885991851629 0.0 46-47 0.0 0.0 0.0 0.11616517715984546 0.0 48-49 0.0 0.0 0.0 0.14455909023176702 0.0 50-51 0.0 0.0 0.0 0.1819481949648733 0.0 52-53 0.0 0.0 0.0 0.2127953421262089 0.0 54-55 0.0 0.0 0.0 0.24682260755159968 0.0 56-57 0.0 0.0 0.0 0.2904356580300712 0.0 58-59 0.0 0.0 0.0 0.34077238712397373 0.0 60-61 0.0 0.0 0.0 0.39824166718154297 0.0 62-63 0.0 0.0 0.0 0.4565741221964986 0.0 64-65 0.0 0.0 0.0 0.5160877639952461 0.0 66-67 0.0 0.0 0.0 0.5874587038928282 0.0 68-69 0.0 0.0 0.0 0.6673705328424442 0.0 70-71 0.0 0.0 0.0 0.7595031019782152 0.0 72-73 0.0 0.0 0.0 0.8565421392928144 0.0 74-75 0.0 0.0 0.0 0.9542626305211397 0.0 76-77 0.0 0.0 0.0 1.0619323488898662 0.0 78-79 0.0 0.0 0.0 1.180687050921871 0.0 80-81 0.0 0.0 0.0 1.3227020465423938 0.0 82-83 0.0 0.0 0.0 1.472440186518479 0.0 84-85 0.0 0.0 0.0 1.6170901372720765 0.0 86-87 0.0 0.0 0.0 1.7739153979509137 0.0 88-89 0.0 0.0 0.0 1.9592254322235023 0.0 90-91 0.0 0.0 0.0 2.170430715217684 0.0 92-93 0.0 0.0 0.0 2.3867696176952684 0.0 94-95 0.0 0.0 0.0 2.6021544846936364 0.0 96-97 0.0 0.0 0.0 2.830168964226395 0.0 98-99 0.0 0.0 0.0 3.074265756123091 0.0 100-101 0.0 0.0 0.0 3.3543433146645247 0.0 102-103 0.0 0.0 0.0 3.66862985967501 0.0 104-105 0.0 0.0 0.0 3.979009402246799 0.0 106-107 0.0 0.0 0.0 4.287571734381984 0.0 108-109 0.0 0.0 0.0 4.616123381319804 0.0 110-111 0.0 0.0 0.0 4.989423835258971 0.0 112-113 0.0 0.0 0.0 5.399522800539348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1255 0.0 48.49639 1 GTATCAA 1915 0.0 46.11539 1 TCAACGC 2130 0.0 40.86673 4 CAACGCA 2130 0.0 40.835102 5 AACGCAG 2205 0.0 39.175976 6 ATCAACG 2265 0.0 38.42396 3 GTACATG 5555 0.0 37.165928 1 TACATGG 5460 0.0 36.49784 2 TATCAAC 2535 0.0 36.010307 2 ACATGGG 5625 0.0 34.971073 3 CATGGGG 3620 0.0 31.7867 4 ACGCAGA 2755 0.0 30.922518 7 GTGGTAT 675 0.0 29.171793 1 CGCAGAG 2975 0.0 28.035059 8 AGTACAT 4070 0.0 25.947304 2 ATGGGGA 1955 0.0 25.901957 5 GAGTACA 4400 0.0 25.630796 1 ATGGGGG 1880 0.0 23.766422 5 GCAGAGT 3505 0.0 23.273182 9 TAAGGTG 590 0.0 23.223959 5 >>END_MODULE