FastQCFastQC Report
Fri 27 May 2016
SRR940079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100588
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.4715661082984731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.3493587064369228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.20743457133823012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.068.6446461
GTATCAA44900.057.518641
TCAACGC57950.044.4507064
ATCAACG58250.044.2217753
CAACGCA58750.043.8670165
AACGCAG60750.042.4074176
TATCAAC61650.041.688122
GTGGTAT9200.037.6016731
ACGCAGA70750.036.1595087
CGCAGAG73200.035.1118058
GCAGAGT79600.032.2149
GTACATG56750.031.5298861
ACATGGG55550.031.0214543
TACATGG56900.031.0204122
TCTCGCG504.4421272E-429.74750168-69
GAGTACT51000.027.53102112-13
CAGAGTA77250.027.07118810-11
TAAGGTG5800.026.6810845
CCGCGTA450.00953472226.44222514-15
TGGTATC12200.025.9050372
Produced by FastQC (version 0.11.5)