##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940079_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100588 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1492075145286 34.0 33.0 34.0 31.0 34.0 2 33.1952547183869 34.0 33.0 34.0 31.0 34.0 3 33.25123933751777 34.0 34.0 34.0 31.0 34.0 4 36.479416457384595 37.0 37.0 37.0 35.0 37.0 5 36.48057220322228 37.0 37.0 37.0 35.0 37.0 6 36.55771642067695 37.0 37.0 37.0 35.0 37.0 7 36.54654148509706 37.0 37.0 37.0 35.0 37.0 8 36.5478244356653 37.0 37.0 37.0 35.0 37.0 9 38.42365808095309 39.0 39.0 39.0 37.0 39.0 10-11 38.401519006203955 39.0 39.0 39.0 37.0 39.0 12-13 38.38006002246072 39.0 39.0 39.0 37.0 39.0 14-15 40.00585914075022 41.0 40.0 41.0 38.0 41.0 16-17 40.00983701439594 41.0 40.0 41.0 38.0 41.0 18-19 40.04170134509917 41.0 40.0 41.0 38.0 41.0 20-21 40.036397816439944 41.0 40.0 41.0 38.0 41.0 22-23 39.9964005604277 41.0 40.0 41.0 38.0 41.0 24-25 39.96165367966941 41.0 40.0 41.0 38.0 41.0 26-27 39.87007672262463 41.0 40.0 41.0 38.0 41.0 28-29 39.78945072997343 41.0 40.0 41.0 37.0 41.0 30-31 39.64282683438307 41.0 40.0 41.0 36.5 41.0 32-33 39.47780595463516 41.0 40.0 41.0 35.5 41.0 34-35 39.36765665262568 41.0 40.0 41.0 35.0 41.0 36-37 39.25676502015286 41.0 39.5 41.0 35.0 41.0 38-39 39.13335735079794 41.0 39.0 41.0 35.0 41.0 40-41 38.988788720211375 41.0 39.0 41.0 35.0 41.0 42-43 38.82477775516361 41.0 39.0 41.0 35.0 41.0 44-45 38.613590644273785 41.0 39.0 41.0 35.0 41.0 46-47 38.30532542604499 41.0 38.0 41.0 35.0 41.0 48-49 38.011843669020564 41.0 38.0 41.0 34.0 41.0 50-51 37.99777300860994 41.0 38.0 41.0 34.0 41.0 52-53 37.875592864904945 41.0 38.0 41.0 34.0 41.0 54-55 37.69856794731544 41.0 37.0 41.0 34.0 41.0 56-57 37.49664770104708 40.0 37.0 41.0 34.0 41.0 58-59 37.2640397678332 40.0 36.0 41.0 33.5 41.0 60-61 37.0109963946545 39.5 35.5 41.0 33.0 41.0 62-63 36.735593155658606 39.0 35.0 41.0 33.0 41.0 64-65 36.42658924138733 39.0 35.0 41.0 33.0 41.0 66-67 36.097924927402445 37.5 35.0 41.0 32.0 41.0 68-69 35.74873976456222 37.0 35.0 40.0 32.0 41.0 70-71 35.37901467215707 36.5 35.0 39.0 32.0 41.0 72-73 35.02425885072343 36.0 35.0 39.0 31.5 41.0 74-75 34.67337096170411 35.5 35.0 38.5 31.0 40.5 76-77 34.325388792172916 35.0 35.0 37.0 31.0 39.0 78-79 34.00711619606973 35.0 35.0 37.0 31.0 39.0 80-81 33.72301033629296 35.0 35.0 36.5 31.0 39.0 82-83 33.47623724772576 35.0 35.0 36.0 30.5 37.0 84-85 33.25671640977369 35.0 35.0 36.0 30.0 37.0 86-87 33.07264525871625 35.0 35.0 35.0 30.0 36.5 88-89 32.9952688926283 35.0 35.0 35.0 30.5 36.0 90-91 33.013901659840016 35.0 35.0 35.0 31.0 36.0 92-93 32.94059993385354 35.0 35.0 35.0 31.0 36.0 94-95 32.87939083471744 35.0 35.0 35.0 31.0 36.0 96-97 32.83158865987999 35.0 35.0 35.0 31.0 35.5 98-99 32.78064089377678 35.0 35.0 35.0 31.0 35.0 100-101 32.727871828513486 35.0 35.0 35.0 30.5 35.0 102-103 32.255658338996966 35.0 33.5 35.0 29.0 35.0 104-105 32.64366638560479 35.0 34.5 35.0 30.0 35.0 106-107 32.690511799147366 35.0 35.0 35.0 31.0 35.0 108-109 32.67197852420706 35.0 35.0 35.0 31.0 35.0 110-111 32.63838829789167 35.0 35.0 35.0 30.5 35.0 112-113 32.61727367552618 35.0 35.0 35.0 30.5 35.0 114-115 32.566189618640216 35.0 35.0 35.0 30.0 35.0 116-117 32.51690232857345 35.0 35.0 35.0 30.0 35.0 118-119 32.4536347843153 35.0 35.0 35.0 30.0 35.0 120-121 32.38587645876568 35.0 35.0 35.0 30.0 35.0 122-123 32.28818277139129 35.0 35.0 35.0 30.0 35.0 124-125 31.238465256753663 34.5 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1447235201304835 1101 2 0.06946817947470407 1101 3 0.09495991484101296 1101 4 -0.1072885673622892 1101 5 -0.052093424578295355 1101 6 -5.213997164830175E-4 1101 7 0.06445712120692093 1101 8 0.07356760244880434 1101 9 0.11201884500962933 1101 10-11 0.09653867132644933 1101 12-13 0.07988377454308448 1101 14-15 0.16331794740353445 1101 16-17 0.13008068597472544 1101 18-19 0.11212758140108292 1101 20-21 0.13359449877104623 1101 22-23 0.10682450440357627 1101 24-25 0.12832136300507813 1101 26-27 0.11799760281486726 1101 28-29 0.11415486333456215 1101 30-31 0.13357373692277008 1101 32-33 0.16505752073788216 1101 34-35 0.15462988059982052 1101 36-37 0.15997683529601403 1101 38-39 0.19857399093407935 1101 40-41 0.20903537537833472 1101 42-43 0.1651625392362135 1101 44-45 0.24547653734135366 1101 46-47 0.05963669466638777 1101 48-49 0.22241870729784807 1101 50-51 0.19495718777123017 1101 52-53 0.12374297533357748 1101 54-55 0.05479646759192747 1101 56-57 -0.0428771217924222 1101 58-59 -0.011445109756778038 1101 60-61 -0.07035677125507078 1101 62-63 -0.035542341469792404 1101 64-65 -0.00746812770385219 1101 66-67 -0.020094234747347173 1101 68-69 -0.05636441639466483 1101 70-71 -0.17123162259532165 1101 72-73 -0.09383326258213742 1101 74-75 -0.033534285015647924 1101 76-77 -0.040722075457232165 1101 78-79 -0.05635844163934678 1101 80-81 -0.1417481751050218 1101 82-83 -0.07238491320623552 1101 84-85 -0.13602515717155228 1101 86-87 -0.1477363731480139 1101 88-89 -0.047154684357217036 1101 90-91 0.012900360887961426 1101 92-93 -0.08899704065768788 1101 94-95 0.01054819906304516 1101 96-97 0.041367021684244776 1101 98-99 0.01904845523471721 1101 100-101 0.04226400578217948 1101 102-103 -0.02544617121994719 1101 104-105 -0.03761741246459138 1101 106-107 0.009356543597000666 1101 108-109 0.07476686007117905 1101 110-111 0.02893380957800673 1101 112-113 -0.05540661563308191 1101 114-115 -0.10139368530572312 1101 116-117 -0.10160500988124 1101 118-119 -0.10129985909405548 1101 120-121 -0.006479291925799657 1101 122-123 -0.16518383124410008 1101 124-125 -0.22418868424759353 1102 1 0.1663625565525635 1102 2 0.183282334925984 1102 3 0.18477486095559215 1102 4 0.012075808099645258 1102 5 0.12368305834510096 1102 6 0.11858729929862477 1102 7 0.06565815594453994 1102 8 0.09639800517143726 1102 9 0.11721401852584279 1102 10-11 0.1357941372162017 1102 12-13 0.13763630337650312 1102 14-15 0.22291983339331978 1102 16-17 0.25162985964563234 1102 18-19 0.2897338160390106 1102 20-21 0.2710568455800342 1102 22-23 0.2759528625740444 1102 24-25 0.24706454138559764 1102 26-27 0.23613569817574387 1102 28-29 0.18792879808765406 1102 30-31 0.115790526915724 1102 32-33 0.12140103600971486 1102 34-35 0.16832468456689043 1102 36-37 0.1874357336957786 1102 38-39 0.24843036093012927 1102 40-41 0.18980807686856593 1102 42-43 0.03540056541404368 1102 44-45 -0.02548248567381961 1102 46-47 0.031662414857521526 1102 48-49 -0.03191758672576128 1102 50-51 -0.151993602032654 1102 52-53 -0.15947831155688874 1102 54-55 -0.11066809006633349 1102 56-57 -0.07116347726432792 1102 58-59 -0.024410752428529747 1102 60-61 0.004820161143214818 1102 62-63 0.07117947825898341 1102 64-65 0.02914919770566371 1102 66-67 0.06978919612976853 1102 68-69 0.0967156790865289 1102 70-71 -0.021668958231892077 1102 72-73 -0.04834165478551 1102 74-75 -0.05739571912418029 1102 76-77 0.05854376788830962 1102 78-79 0.04308411925032374 1102 80-81 0.03361417643075981 1102 82-83 -0.07101360654920086 1102 84-85 -0.11280667412783885 1102 86-87 -0.07049549800405686 1102 88-89 -0.06915252722148324 1102 90-91 -0.05143100223275354 1102 92-93 -0.042744580577583235 1102 94-95 -0.08620949056723504 1102 96-97 -0.10629257198357323 1102 98-99 -0.13361482318189388 1102 100-101 -0.08079210123238312 1102 102-103 0.0021572166004020232 1102 104-105 -0.007162372845485265 1102 106-107 0.0447215378389032 1102 108-109 0.03898290022312523 1102 110-111 -0.02802644455148595 1102 112-113 0.06035653558986809 1102 114-115 0.009754935760298622 1102 116-117 0.009157679405269903 1102 118-119 -0.08593402692093832 1102 120-121 0.01466853417984737 1102 122-123 0.1103002888851563 1102 124-125 0.19176520375323491 1103 1 0.21152444106957802 1103 2 0.19870487341748344 1103 3 0.18941215579459225 1103 4 0.09619816789571445 1103 5 0.12357296162937104 1103 6 0.13611596678960325 1103 7 0.14204198697148485 1103 8 0.15974172711794665 1103 9 0.2327943975973028 1103 10-11 0.18690918404965373 1103 12-13 0.21492128660793242 1103 14-15 0.2584363457834371 1103 16-17 0.2591706907064619 1103 18-19 0.2781310513410986 1103 20-21 0.2040357345347914 1103 22-23 0.24126234020747717 1103 24-25 0.21151944619428065 1103 26-27 0.26593326842591125 1103 28-29 0.2920977584358866 1103 30-31 0.2853868424867869 1103 32-33 0.30996627466551274 1103 34-35 0.26642509277163384 1103 36-37 0.2627561786558914 1103 38-39 0.2526145654390177 1103 40-41 0.3374075450167382 1103 42-43 0.37639311906502115 1103 44-45 0.4856902137282617 1103 46-47 0.38788166188067663 1103 48-49 0.2758138492741651 1103 50-51 0.2810718292924008 1103 52-53 0.37415464716531943 1103 54-55 0.33566081963742533 1103 56-57 0.2874233784581577 1103 58-59 0.2889819307978101 1103 60-61 0.3107942841805311 1103 62-63 0.32062740674692947 1103 64-65 0.3317195855001742 1103 66-67 0.2770523505700595 1103 68-69 0.2588375921439905 1103 70-71 0.28286649714506495 1103 72-73 0.26228723035821133 1103 74-75 0.10679103666436163 1103 76-77 0.213095208461489 1103 78-79 0.15647791403004163 1103 80-81 0.1613004304323482 1103 82-83 0.21031049700946625 1103 84-85 0.1890634350372835 1103 86-87 0.24819664828907406 1103 88-89 0.23116696497859124 1103 90-91 0.16556830119379384 1103 92-93 0.133725898681881 1103 94-95 0.13845688462543393 1103 96-97 0.14990142689852348 1103 98-99 0.1843230547462511 1103 100-101 0.22220941048021103 1103 102-103 0.2107280341955473 1103 104-105 0.15707281491175706 1103 106-107 0.12250910199141885 1103 108-109 0.09812812626014988 1103 110-111 0.12375802207226627 1103 112-113 0.08412604599824647 1103 114-115 0.05989419751947622 1103 116-117 0.13713558384132085 1103 118-119 0.17859715162205703 1103 120-121 0.2330149344406891 1103 122-123 0.22630491082996684 1103 124-125 0.22009552354185047 1104 1 0.11347810009729642 1104 2 0.13556153279047578 1104 3 0.138487919417436 1104 4 0.04796777682743425 1104 5 0.0411637611434017 1104 6 0.04441703765005656 1104 7 0.09595310974695792 1104 8 0.1252776931104762 1104 9 0.16235790044515142 1104 10-11 0.15081196750000458 1104 12-13 0.14711729336014656 1104 14-15 0.20522513619823712 1104 16-17 0.21231206944382564 1104 18-19 0.25984717160337567 1104 20-21 0.2723881768803409 1104 22-23 0.25499401478640493 1104 24-25 0.23946110301267964 1104 26-27 0.2746872303818151 1104 28-29 0.26806538673907454 1104 30-31 0.19230604938642415 1104 32-33 0.17034624972519197 1104 34-35 0.15483317202991032 1104 36-37 0.19552289341214646 1104 38-39 0.12734713503304107 1104 40-41 0.1501785666125386 1104 42-43 0.15073672721302245 1104 44-45 0.07010064518351555 1104 46-47 0.12174537836225596 1104 48-49 0.03300231129244935 1104 50-51 -0.0761973342023694 1104 52-53 -0.04398900146284035 1104 54-55 -0.08806619092416668 1104 56-57 -0.1634478506631183 1104 58-59 -0.11761918080161848 1104 60-61 -0.07269147089591854 1104 62-63 -0.04129438580937972 1104 64-65 -0.0968407136181142 1104 66-67 -0.07122990892634817 1104 68-69 -0.05736984222717467 1104 70-71 -0.06619456524664713 1104 72-73 -0.11545779749389595 1104 74-75 -0.20979583441152982 1104 76-77 -0.15779666450124097 1104 78-79 -0.18325563133230816 1104 80-81 -0.20370163239884675 1104 82-83 -0.1687387474559614 1104 84-85 -0.2310245031719944 1104 86-87 -0.2188001231342227 1104 88-89 -0.17066841125645738 1104 90-91 -0.17963317037873594 1104 92-93 -0.20967493090237355 1104 94-95 -0.2621097339516396 1104 96-97 -0.2824084626300092 1104 98-99 -0.20928170265495538 1104 100-101 -0.1791463639718387 1104 102-103 -0.2536825629073789 1104 104-105 -0.2552889533978657 1104 106-107 -0.2682071740832086 1104 108-109 -0.288166651023964 1104 110-111 -0.2818530858466701 1104 112-113 -0.14686963629158356 1104 114-115 -0.12530249097645196 1104 116-117 -0.09973204969011107 1104 118-119 -0.17364796966568719 1104 120-121 -0.26985594682047065 1104 122-123 -0.10872475822149852 1104 124-125 -0.16498044921117838 1105 1 0.08149515766659476 1105 2 0.07728150436119563 1105 3 0.06528251785382366 1105 4 0.04157118834129392 1105 5 0.03334570854922703 1105 6 0.10198633402532664 1105 7 0.06965602374837943 1105 8 0.0947162147878089 1105 9 0.07635932190925843 1105 10-11 0.11776292698827007 1105 12-13 0.15209241773825255 1105 14-15 0.1437468135258797 1105 16-17 0.21160133738980846 1105 18-19 0.2378144779289002 1105 20-21 0.20024887339775432 1105 22-23 0.19458179019507327 1105 24-25 0.22240353371629595 1105 26-27 0.2075230512773416 1105 28-29 0.2264875615791624 1105 30-31 0.22855338414122173 1105 32-33 0.1550655105638583 1105 34-35 0.1765104996773843 1105 36-37 0.14754847976609398 1105 38-39 0.18989155578641714 1105 40-41 0.18287224109725742 1105 42-43 0.1518028252940482 1105 44-45 0.1330004319639002 1105 46-47 0.17611638049445588 1105 48-49 0.26185803268548113 1105 50-51 0.3921750893939304 1105 52-53 0.304625071031829 1105 54-55 0.32984425683702057 1105 56-57 0.3017262786901185 1105 58-59 0.37918252495531846 1105 60-61 0.39177192640329395 1105 62-63 0.3464099496561488 1105 64-65 0.3598046310869307 1105 66-67 0.2777395295953369 1105 68-69 0.2855583738353715 1105 70-71 0.30800586119258355 1105 72-73 0.24666018402707124 1105 74-75 0.2635517135628831 1105 76-77 0.31306332128411896 1105 78-79 0.3554792656115424 1105 80-81 0.1975066120929796 1105 82-83 0.27084902795270693 1105 84-85 0.3158838123930394 1105 86-87 0.20906057338319073 1105 88-89 0.3421247657158304 1105 90-91 0.34915147780889555 1105 92-93 0.3159114230421878 1105 94-95 0.34094641388062286 1105 96-97 0.2655659510160433 1105 98-99 0.27330536202165234 1105 100-101 0.3204982700011598 1105 102-103 0.39343897227386293 1105 104-105 0.3278666969219586 1105 106-107 0.2747921151847308 1105 108-109 0.29854265956594617 1105 110-111 0.28104670093371453 1105 112-113 0.2802803281718127 1105 114-115 0.37682970518559955 1105 116-117 0.34305401996162743 1105 118-119 0.3906732812227318 1105 120-121 0.2795399309336162 1105 122-123 0.27293835478489825 1105 124-125 0.2689214697653668 1106 1 0.13178647889331074 1106 2 0.12328130608619148 1106 3 0.14409584686653432 1106 4 0.14358170678111293 1106 5 0.08825062285940533 1106 6 0.11596274916387017 1106 7 0.10755614345321618 1106 8 0.13407774953500962 1106 9 0.09455130045215299 1106 10-11 0.1361960908014055 1106 12-13 0.1012499215841487 1106 14-15 0.17360890388700767 1106 16-17 0.2061796010235497 1106 18-19 0.2590811987512751 1106 20-21 0.261526208344911 1106 22-23 0.2663427928550206 1106 24-25 0.2077913561782978 1106 26-27 0.23479770211357476 1106 28-29 0.2618221901119213 1106 30-31 0.23720547414229287 1106 32-33 0.22572564303325748 1106 34-35 0.18521783047654594 1106 36-37 0.22549423231592414 1106 38-39 0.25157185262678894 1106 40-41 0.1976696232752886 1106 42-43 0.19388299646161045 1106 44-45 0.13179080645213048 1106 46-47 0.16900067519029704 1106 48-49 0.1285052664285402 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-0.5692037702197652 2216 106-107 -0.546948351129771 2216 108-109 -0.5838535541398713 2216 110-111 -0.6681140106086758 2216 112-113 -0.6673247073901152 2216 114-115 -0.7116606560384824 2216 116-117 -0.7071543038755834 2216 118-119 -0.6716432325774591 2216 120-121 -0.6857865584383234 2216 122-123 -0.7669757496167477 2216 124-125 -0.7873398939285501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 3.0 4 20.0 5 50.0 6 119.0 7 248.0 8 477.0 9 869.0 10 1522.0 11 2316.0 12 2904.0 13 4573.0 14 8395.0 15 8879.0 16 3934.0 17 3257.0 18 3119.0 19 2487.0 20 2065.0 21 1962.0 22 1925.0 23 2099.0 24 2561.0 25 3354.0 26 4129.0 27 5145.0 28 6345.0 29 7751.0 30 9748.0 31 11895.0 32 15245.0 33 20675.0 34 30621.0 35 57075.0 36 153052.0 37 446613.0 38 272935.0 39 2213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70520024245927 18.079482656619327 13.614251661454881 23.601065439466517 2 15.726380943381141 19.482677783512273 40.417535927425504 24.373405345681075 3 19.25192579225976 24.650595228360164 29.09913446109618 26.998344518283897 4 12.910278491239657 16.163664667760287 36.30485278423002 34.62120405677003 5 13.915039244470098 37.3421017947471 33.0412532779472 15.7016056828356 6 32.087487289371595 35.92861874657529 16.921242720069934 15.062651243983183 7 28.692844188742743 32.85207543603965 20.16367614402483 18.291404231192782 8 26.745975787488142 34.71089999164083 19.195648144446423 19.347476076424602 9 27.376184366902056 15.919672029860402 19.363740109832197 37.34040349340535 10-11 25.877031186965517 26.04312422087103 26.277180925105487 21.802663667057974 12-13 26.530182048141537 23.331800819198463 27.36664401210989 22.771373120550106 14-15 23.123048770293696 24.31500252592251 25.64692691543066 26.915021788353133 16-17 22.689189778554734 28.05454902288595 26.060296859496923 23.195964339062392 18-19 22.75020261896368 25.744783697441733 29.099808466019983 22.405205217574604 20-21 23.31467361083348 25.035753615340163 29.21006770926087 22.439505064565488 22-23 23.154077638498695 24.705611909270317 29.298838439088925 22.841472013142067 24-25 22.912206929386837 25.16618389442734 29.191759314112094 22.729849862073728 26-27 22.522778732822818 25.4692491649918 29.070006214859696 22.937965887325685 28-29 22.46063572525715 25.46417338771045 29.465225554943252 22.609965332089146 30-31 22.600086408474027 25.07703846994307 29.248178130478614 23.074696991104286 32-33 22.508523856576602 25.206355696389426 29.172328415505355 23.112792031528613 34-35 22.99108745092374 25.479950171863457 28.63764023249496 22.891322144717847 36-37 23.281693660132206 25.061399722872135 28.712854644163276 22.944051972832384 38-39 22.808747926052057 25.089272896112696 28.85104646915474 23.250932708680505 40-41 22.929470428534565 25.076731710685564 28.89805267729614 23.095745183483736 42-43 23.252963442845488 24.982191175909385 28.49366423824572 23.271181142999406 44-45 22.988253006711464 25.324658509448888 28.417029392242902 23.270059091596746 46-47 23.275352152987452 25.79864199740684 27.8506378868731 23.075367962732614 48-49 23.5720126843363 25.77050982672615 27.374087972596016 23.283389516341533 50-51 23.489309659604586 26.021172607359983 27.249260158117632 23.240257574917795 52-53 23.751031266922773 25.79534757784021 27.171066738870497 23.282554416366523 54-55 23.25225243233617 25.900518631858603 27.296953991866168 23.55027494393906 56-57 23.308550776981978 26.317348470909458 27.224921574907608 23.149179177200956 58-59 23.316233072377173 25.684055869332926 27.3450618226225 23.654649235667403 60-61 23.23860414910011 25.697044093274794 27.874767394527954 23.189584363097143 62-63 22.981201386715174 25.92027069203556 27.72594430333281 23.372583617916447 64-65 22.93965186300766 25.768134311914935 27.54988880787045 23.74232501720695 66-67 22.95316642709558 26.074480863691623 27.41427686179444 23.558075847418355 68-69 22.889968525572062 25.85255357194778 27.703084566626927 23.554393335853227 70-71 23.011534002318797 25.878088848344316 27.56434835703616 23.54602879230073 72-73 23.038639345513488 25.983746869855022 27.29940722595558 23.678206558675907 74-75 23.049675354765554 26.33469806085122 26.94759882462615 23.668027759757074 76-77 23.38386596434669 26.1722622378226 26.925708491172085 23.51816330665862 78-79 23.309147524047624 26.251968854504888 27.006300788723003 23.432582832724485 80-81 23.222223029871305 26.61604524127103 26.89008057511076 23.271651153746905 82-83 23.375232148633277 26.351368541179802 26.928514575844915 23.344884734342006 84-85 23.27351809290254 26.39377203092723 26.727276939914148 23.605432936256086 86-87 23.307136623436566 26.021742103166183 27.021984247446923 23.649137025950324 88-89 23.11423529967617 26.2359302481946 26.875997194227086 23.773837257902137 90-91 23.064450694652983 26.58993781037457 26.923669265132062 23.42194222984039 92-93 23.10436315397336 26.012466154209594 27.06254883788548 23.820621853931563 94-95 23.130534888337966 25.919802866772883 27.033265777768083 23.916396467121064 96-97 23.321569860310273 26.046173751705915 26.887501340200004 23.74475504778381 98-99 23.189785823577942 25.99937487960981 26.71272083649831 24.09811846031394 100-101 22.802555004838336 25.962465416119606 27.04130076277616 24.193678816265894 102-103 22.970902826489112 25.467931687425267 27.192736973326987 24.368428512758637 104-105 23.031961097158973 25.605857959563433 27.224447295445707 24.137733647831887 106-107 23.178183955888894 25.70838495363146 26.81570565458627 24.297725435893383 108-109 23.303841374908288 25.526800580601638 26.709083598912393 24.46027444557768 110-111 22.921656423657172 25.859676827295953 27.048359604138877 24.170307144907994 112-113 22.977905851022022 25.81335116626559 26.975550740946524 24.23319224176586 114-115 22.66174859117945 25.81876052867343 26.887029037786014 24.63246184236111 116-117 22.636079199950206 25.832525805676365 27.02348712930392 24.507907865069512 118-119 22.66158452095931 26.054552806007713 26.652462099701523 24.631400573331454 120-121 22.548603608801486 26.067543956088883 26.907822825455867 24.476029609653757 122-123 22.561092509892468 25.951562123779624 26.839271841793227 24.64807352453468 124-125 22.653131966506706 26.009594045313445 26.689463768372722 24.647810219807127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 92.0 1 81.0 2 210.0 3 354.5 4 226.0 5 148.5 6 369.5 7 1001.5 8 1940.0 9 2229.0 10 1549.5 11 986.5 12 951.0 13 1070.0 14 1209.0 15 1352.0 16 1498.5 17 1578.5 18 1720.0 19 1825.5 20 1933.0 21 2189.0 22 2460.0 23 2892.5 24 3431.5 25 4056.0 26 4894.0 27 5705.5 28 6565.0 29 7877.0 30 9445.0 31 11428.5 32 13470.5 33 14656.5 34 15767.5 35 16673.0 36 18575.0 37 20947.0 38 22895.0 39 24037.0 40 25228.0 41 29065.0 42 32641.0 43 35175.5 44 37718.0 45 40656.0 46 42382.5 47 42468.5 48 43139.5 49 44029.5 50 44218.0 51 43603.5 52 43224.5 53 43412.5 54 42431.5 55 39724.5 56 35530.0 57 31696.5 58 29801.0 59 27100.0 60 23486.5 61 20238.0 62 16694.0 63 12835.0 64 10106.5 65 8143.5 66 6089.0 67 5042.5 68 3860.5 69 2488.5 70 1912.0 71 1489.0 72 1208.0 73 1045.5 74 855.5 75 690.0 76 464.0 77 216.0 78 97.5 79 57.0 80 33.5 81 19.5 82 13.0 83 6.5 84 2.0 85 0.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017899522800539348 2 0.003270978785885363 3 1.8172104366029796E-4 4 0.13510959596143152 5 0.03934260595245451 6 0.011902728359749516 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.2715130457537242E-4 30-31 1.3629078274522347E-4 32-33 4.543026091507449E-5 34-35 4.5430260915074484E-4 36-37 0.0011811867837919366 38-39 0.002725815654904469 40-41 0.0 42-43 9.086052183014897E-4 44-45 7.723144355562664E-4 46-47 9.086052183014898E-5 48-49 0.0016354893929426816 50-51 0.0013629078274522346 52-53 0.0 54-55 0.0 56-57 0.001408338088367309 58-59 0.0018172104366029793 60-61 0.0010903262619617877 62-63 1.3629078274522347E-4 64-65 0.001408338088367309 66-67 9.086052183014897E-4 68-69 0.0010903262619617877 70-71 0.0014537683492823836 72-73 0.0 74-75 1.8172104366029796E-4 76-77 0.004088723482356704 78-79 0.001044896001046713 80-81 0.0 82-83 0.0 84-85 4.088723482356704E-4 86-87 4.088723482356704E-4 88-89 0.0 90-91 2.2715130457537242E-4 92-93 7.268841746411918E-4 94-95 0.0011357565228768622 96-97 5.451631309808939E-4 98-99 0.0 100-101 2.7258156549044693E-4 102-103 0.0 104-105 0.0 106-107 3.180118264055214E-4 108-109 4.997328700658193E-4 110-111 0.0 112-113 2.2715130457537242E-4 114-115 0.0019989314802632773 116-117 5.905933918959683E-4 118-119 2.7258156549044693E-4 120-121 9.086052183014898E-5 122-123 2.7258156549044693E-4 124-125 8.631749573864152E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1100588.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.65065499016775 #Duplication Level Percentage of deduplicated Percentage of total 1 66.95131750406665 28.55517544003126 2 15.808765753917896 13.485084279814629 3 6.4563720942382785 8.26105496038511 4 3.317870668790903 5.6603742878639185 5 1.8899007621314963 4.030275268566277 6 1.1994778089457012 3.069510851862327 7 0.8185964213117782 2.4439571479088262 8 0.5943388413490264 2.0279152695706695 9 0.44223372541769634 1.6975402243026072 >10 2.2767581120714397 17.619132156410156 >50 0.15859603319819002 4.673021904759572 >100 0.08130275188549974 6.164185847884886 >500 0.003405350609894886 1.0967468271155192 >1k 8.513376524737215E-4 0.7449018757912556 >5k 2.1283441311843038E-4 0.47112365773295345 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.4715661082984731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3845 0.3493587064369228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2283 0.20743457133823012 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.543026091507449E-5 0.0 24-25 0.0 0.0 0.0 1.8172104366029796E-4 0.0 26-27 0.0 0.0 0.0 4.543026091507449E-4 0.0 28-29 0.0 0.0 0.0 9.540354792165642E-4 0.0 30-31 0.0 0.0 0.0 0.0033164090468004374 0.0 32-33 0.0 0.0 0.0 0.006996260180921471 0.0 34-35 0.0 0.0 0.0 0.011993588881579665 0.0 36-37 0.0 0.0 0.0 0.019898454280802627 0.0 38-39 0.0 0.0 0.0 0.0350721614264375 0.0 40-41 0.0 0.0 0.0 0.053698568401618046 0.0 42-43 0.0 0.0 0.0 0.07146180041941216 0.0 44-45 0.0 0.0 0.0 0.09213256913577106 0.0 46-47 0.0 0.0 0.0 0.11630146794259069 0.0 48-49 0.0 0.0 0.0 0.14474081127542732 0.0 50-51 0.0 0.0 0.0 0.182357067313109 0.0 52-53 0.0 0.0 0.0 0.21361308682268026 0.0 54-55 0.0 0.0 0.0 0.2478675035526464 0.0 56-57 0.0 0.0 0.0 0.29193485664026864 0.0 58-59 0.0 0.0 0.0 0.3423624462560013 0.0 60-61 0.0 0.0 0.0 0.40005887761814596 0.0 62-63 0.0 0.0 0.0 0.45830047211127145 0.0 64-65 0.0 0.0 0.0 0.517223520518123 0.0 66-67 0.0 0.0 0.0 0.5882764485892995 0.0 68-69 0.0 0.0 0.0 0.6682337077998306 0.0 70-71 0.0 0.0 0.0 0.7608205795447525 0.0 72-73 0.0 0.0 0.0 0.858132198424842 0.0 74-75 0.0 0.0 0.0 0.9560798409577427 0.0 76-77 0.0 0.0 0.0 1.0645218737620255 0.0 78-79 0.0 0.0 0.0 1.1829585639676246 0.0 80-81 0.0 0.0 0.0 1.324655547761742 0.0 82-83 0.0 0.0 0.0 1.4743028272159973 0.0 84-85 0.0 0.0 0.0 1.6196796621442355 0.0 86-87 0.0 0.0 0.0 1.777186376736799 0.0 88-89 0.0 0.0 0.0 1.9637230280540947 0.0 90-91 0.0 0.0 0.0 2.176018637310238 0.0 92-93 0.0 0.0 0.0 2.393266145006124 0.0 94-95 0.0 0.0 0.0 2.610468222441095 4.543026091507449E-5 96-97 0.0 0.0 0.0 2.840527063715032 9.086052183014898E-5 98-99 0.0 0.0 0.0 3.0851690187427083 9.086052183014898E-5 100-101 0.0 0.0 0.0 3.3688809981573486 9.086052183014898E-5 102-103 0.0 0.0 0.0 3.6866202429973796 9.086052183014898E-5 104-105 0.0 0.0 0.0 3.9994530196585827 9.086052183014898E-5 106-107 0.0 0.0 0.0 4.3111046095359935 9.086052183014898E-5 108-109 0.0 0.0 0.0 4.643245247086103 9.086052183014898E-5 110-111 0.0 0.0 0.0 5.01922608641926 9.086052183014898E-5 112-113 0.0 0.0 0.0 5.431460273962646 9.086052183014898E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2485 0.0 68.644646 1 GTATCAA 4490 0.0 57.51864 1 TCAACGC 5795 0.0 44.450706 4 ATCAACG 5825 0.0 44.221775 3 CAACGCA 5875 0.0 43.867016 5 AACGCAG 6075 0.0 42.407417 6 TATCAAC 6165 0.0 41.68812 2 GTGGTAT 920 0.0 37.601673 1 ACGCAGA 7075 0.0 36.159508 7 CGCAGAG 7320 0.0 35.111805 8 GCAGAGT 7960 0.0 32.214 9 GTACATG 5675 0.0 31.529886 1 ACATGGG 5555 0.0 31.021454 3 TACATGG 5690 0.0 31.020412 2 TCTCGCG 50 4.4421272E-4 29.747501 68-69 GAGTACT 5100 0.0 27.531021 12-13 CAGAGTA 7725 0.0 27.071188 10-11 TAAGGTG 580 0.0 26.681084 5 CCGCGTA 45 0.009534722 26.442225 14-15 TGGTATC 1220 0.0 25.905037 2 >>END_MODULE