FastQCFastQC Report
Fri 27 May 2016
SRR940078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483279
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.16160437345715414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.060.8263131
GTATCAA9850.055.154221
ATCAACG11400.045.5178833
TCAACGC11500.045.1314434
CAACGCA11500.045.084645
AACGCAG11900.043.569196
TATCAAC12450.043.1520652
GTACTAG705.3405063E-542.642781
CTCGTAC650.001914903236.704083
AGGACCG1304.1358362E-836.6736265
ACGCAGA14900.034.3969047
GTACATG25300.033.979391
TACATGG25850.033.483892
CGCAGAG15600.032.8534558
GGACCGC1152.730859E-531.0928556
ACATGGG26900.031.0415533
CGTTACA400.005379896629.72948534-35
GCAGAGT18000.027.79939
CATGGGG13750.026.8996644
GTAAGGT2005.947004E-826.8454284
Produced by FastQC (version 0.11.5)