FastQCFastQC Report
Fri 27 May 2016
SRR940078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483279
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.6133103238501982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.4423945588366141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.277272548569253No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.11794429304811506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.073.946821
GTATCAA20550.057.1337741
CCCGCTA350.0077965950.994949
ATCAACG26200.043.898483
TCAACGC26850.042.835764
CAACGCA27400.042.1453635
GTGGTAT4500.041.057041
TATCAAC28100.040.9302562
AACGCAG28500.040.501936
TCTAGCG300.001308713639.664787118-119
TGGTATC4750.037.637532
ACGCAGA33500.034.6308987
CGCAGAG33650.034.299728
GTATAAC1407.932431E-834.056531
GTACATG25950.033.5315741
TACATGG26250.032.4638632
TAACGCA750.003878567131.7828034
TATAACG750.003878567131.7828032
ATAACGC953.2536566E-431.3646093
GCAGAGT36500.031.2955289
Produced by FastQC (version 0.11.5)