##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940078_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 483279 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.11281475089958 34.0 33.0 34.0 31.0 34.0 2 33.163990158893725 34.0 33.0 34.0 31.0 34.0 3 33.218906677095426 34.0 33.0 34.0 31.0 34.0 4 36.452744687851116 37.0 37.0 37.0 35.0 37.0 5 36.45371514177111 37.0 37.0 37.0 35.0 37.0 6 36.53657618063272 37.0 37.0 37.0 35.0 37.0 7 36.526679206007294 37.0 37.0 37.0 35.0 37.0 8 36.53067275838594 37.0 37.0 37.0 35.0 37.0 9 38.39979183866876 39.0 39.0 39.0 37.0 39.0 10-11 38.375550148051126 39.0 39.0 39.0 37.0 39.0 12-13 38.351926113073404 39.0 39.0 39.0 37.0 39.0 14-15 39.954956660645294 41.0 40.0 41.0 38.0 41.0 16-17 39.962347836342985 41.0 40.0 41.0 38.0 41.0 18-19 40.001300491020714 41.0 40.0 41.0 38.0 41.0 20-21 39.99557812360976 41.0 40.0 41.0 38.0 41.0 22-23 39.95272296127082 41.0 40.0 41.0 38.0 41.0 24-25 39.91014610607951 41.0 40.0 41.0 38.0 41.0 26-27 39.818852050264965 41.0 40.0 41.0 37.0 41.0 28-29 39.7386592423838 41.0 40.0 41.0 37.0 41.0 30-31 39.58647592798363 41.0 40.0 41.0 36.5 41.0 32-33 39.40327429911086 41.0 40.0 41.0 35.0 41.0 34-35 39.28900283273223 41.0 39.0 41.0 35.0 41.0 36-37 39.18558327591309 41.0 39.0 41.0 35.0 41.0 38-39 39.06789245963512 41.0 39.0 41.0 35.0 41.0 40-41 38.927224232793066 41.0 39.0 41.0 35.0 41.0 42-43 38.757801394225694 41.0 39.0 41.0 35.0 41.0 44-45 38.54228820205306 41.0 38.5 41.0 35.0 41.0 46-47 38.22997688705696 41.0 38.0 41.0 34.5 41.0 48-49 37.93049977342281 41.0 38.0 41.0 34.0 41.0 50-51 37.930901197858795 41.0 38.0 41.0 34.0 41.0 52-53 37.81491333163659 41.0 38.0 41.0 34.0 41.0 54-55 37.64315850678386 40.5 37.0 41.0 34.0 41.0 56-57 37.44123373868925 40.0 37.0 41.0 34.0 41.0 58-59 37.204275377163086 40.0 36.0 41.0 33.0 41.0 60-61 36.948005189548894 39.5 35.5 41.0 33.0 41.0 62-63 36.665133390857044 39.0 35.0 41.0 33.0 41.0 64-65 36.3491699411727 39.0 35.0 41.0 32.5 41.0 66-67 36.02246528402848 37.5 35.0 41.0 32.0 41.0 68-69 35.66965872715346 37.0 35.0 40.0 32.0 41.0 70-71 35.301501823998144 36.5 35.0 39.0 31.5 41.0 72-73 34.94901702743136 36.0 35.0 39.0 31.0 41.0 74-75 34.601786959499584 35.5 35.0 38.5 31.0 40.5 76-77 34.25816660769452 35.0 35.0 37.0 31.0 39.0 78-79 33.94813968742693 35.0 35.0 37.0 31.0 39.0 80-81 33.66325352436171 35.0 35.0 36.5 30.5 39.0 82-83 33.41237877085493 35.0 35.0 36.0 30.0 37.0 84-85 33.19972624508824 35.0 35.0 36.0 30.0 37.0 86-87 33.01334322410037 35.0 35.0 35.0 30.0 36.5 88-89 32.91731380837984 35.0 35.0 35.0 30.0 36.0 90-91 32.930632202102714 35.0 35.0 35.0 31.0 36.0 92-93 32.85623832196309 35.0 35.0 35.0 30.5 36.0 94-95 32.79051438196156 35.0 35.0 35.0 30.0 36.0 96-97 32.7322064480352 35.0 35.0 35.0 30.0 35.5 98-99 32.68187630747457 35.0 35.0 35.0 30.0 35.0 100-101 32.627856786659464 35.0 35.0 35.0 30.0 35.0 102-103 32.14996409941256 35.0 33.5 35.0 28.5 35.0 104-105 32.538842987177176 35.0 34.5 35.0 30.0 35.0 106-107 32.58551788925238 35.0 35.0 35.0 30.0 35.0 108-109 32.57171116477232 35.0 35.0 35.0 30.0 35.0 110-111 32.53231984009237 35.0 35.0 35.0 30.0 35.0 112-113 32.50620035217752 35.0 35.0 35.0 30.0 35.0 114-115 32.45289056631883 35.0 35.0 35.0 30.0 35.0 116-117 32.40190138615583 35.0 35.0 35.0 30.0 35.0 118-119 32.33611743940871 35.0 35.0 35.0 29.0 35.0 120-121 32.26423660039025 35.0 35.0 35.0 29.0 35.0 122-123 32.158395047167375 35.0 35.0 35.0 29.0 35.0 124-125 31.100250579892773 34.5 32.5 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2450830626435092 1101 2 0.1762581478051004 1101 3 0.16225749016763302 1101 4 0.16261050387024767 1101 5 0.17035404992955705 1101 6 0.18647218296820256 1101 7 0.18708852871890258 1101 8 0.1615585757318101 1101 9 0.23059808703403917 1101 10-11 0.08238297293310382 1101 12-13 0.14090028087095163 1101 14-15 0.26054027354164333 1101 16-17 0.2123055134353251 1101 18-19 0.09175075974056313 1101 20-21 0.14741072961054158 1101 22-23 0.231120200910631 1101 24-25 0.1912665392052375 1101 26-27 0.18707402573455312 1101 28-29 0.25040265604371825 1101 30-31 0.19766265935637506 1101 32-33 0.11198340254739492 1101 34-35 -0.04332573683306862 1101 36-37 -9.016365664038517E-4 1101 38-39 0.1742752982795608 1101 40-41 0.027150580537671942 1101 42-43 -0.08208106388552494 1101 44-45 -0.09524458094914223 1101 46-47 -0.11691594256449633 1101 48-49 -0.11329159883312201 1101 50-51 -0.3393420398996838 1101 52-53 -0.35770458467217026 1101 54-55 -0.3947121767436599 1101 56-57 -0.417661273320185 1101 58-59 -0.4332728468274851 1101 60-61 -0.4243177547788619 1101 62-63 -0.4769070132336424 1101 64-65 -0.5027983797906117 1101 66-67 -0.31487617417698743 1101 68-69 -0.3703477131613724 1101 70-71 -0.27800204512978155 1101 72-73 -0.25522914959994836 1101 74-75 -0.22199294589324126 1101 76-77 -0.30310422397568715 1101 78-79 -0.24090790395890593 1101 80-81 -0.3482466183490729 1101 82-83 -0.3252643029848983 1101 84-85 -0.3528378999731956 1101 86-87 -0.44026763176533734 1101 88-89 -0.5156096825595213 1101 90-91 -0.4541626191677963 1101 92-93 -0.4568884490164322 1101 94-95 -0.3939136011818718 1101 96-97 -0.4790938567599454 1101 98-99 -0.462391661945297 1101 100-101 -0.44333111291816607 1101 102-103 -0.3882850040427641 1101 104-105 -0.21683143993818987 1101 106-107 -0.3110627434342774 1101 108-109 -0.434441661461193 1101 110-111 -0.31033698364795725 1101 112-113 -0.3773972997054855 1101 114-115 -0.3237929903680197 1101 116-117 -0.3351836617702375 1101 118-119 -0.6304959242331236 1101 120-121 -0.3690236299260121 1101 122-123 -0.47390188553665524 1101 124-125 -0.5274573279751884 1102 1 0.13279220035264672 1102 2 0.11640109687662203 1102 3 0.16130373028529732 1102 4 0.057041925087382594 1102 5 0.02080909681317422 1102 6 0.0615641116315544 1102 7 0.12138251658431898 1102 8 0.05541544280296051 1102 9 0.030262547412782226 1102 10-11 0.09863136418148599 1102 12-13 0.1459621028970588 1102 14-15 0.28012107883672854 1102 16-17 0.21015668092935158 1102 18-19 0.30050926314191884 1102 20-21 0.22485488294754674 1102 22-23 0.23562470541514102 1102 24-25 0.16339491758361646 1102 26-27 0.23676146827914124 1102 28-29 0.31198336941015015 1102 30-31 0.23284260971488635 1102 32-33 0.3162546380686351 1102 34-35 0.3264917847701696 1102 36-37 0.249305202926152 1102 38-39 0.390319148251983 1102 40-41 0.39540531486382946 1102 42-43 0.3767578770962814 1102 44-45 0.3491730509684956 1102 46-47 0.22922420268279353 1102 48-49 0.10727399226818335 1102 50-51 0.13703803915896628 1102 52-53 0.01992687331642884 1102 54-55 0.1295914636314066 1102 56-57 0.14200042919866007 1102 58-59 0.16264896784432636 1102 60-61 0.09307399922003157 1102 62-63 0.024914323409127803 1102 64-65 0.035185394800308245 1102 66-67 -0.037906602564561354 1102 68-69 0.032581363874847113 1102 70-71 0.019553801533206183 1102 72-73 -0.06344297567091672 1102 74-75 0.17128936703193176 1102 76-77 0.1335339448053432 1102 78-79 0.08254309806352467 1102 80-81 0.012481456664716006 1102 82-83 -0.04921976354749802 1102 84-85 0.06597759919944934 1102 86-87 0.080887451889744 1102 88-89 0.18409959304261037 1102 90-91 0.2906606479768996 1102 92-93 0.25135295565354454 1102 94-95 0.21549874483761755 1102 96-97 0.2613800354996627 1102 98-99 0.17694346399697025 1102 100-101 0.059340563860658335 1102 102-103 0.16784319047828689 1102 104-105 0.17345170034494828 1102 106-107 0.0904988947345089 1102 108-109 0.12431224550699937 1102 110-111 0.182814330931933 1102 112-113 0.15358265984590957 1102 114-115 0.2332832184939022 1102 116-117 0.2214788681065798 1102 118-119 0.22091862807427987 1102 120-121 0.17949746770222674 1102 122-123 -0.11827010579774111 1102 124-125 -0.10428670837599796 1103 1 0.07001537670625879 1103 2 -0.008672346131604058 1103 3 -0.0064909456615538375 1103 4 -0.16555253392012048 1103 5 0.019306339875640788 1103 6 0.033689665278849645 1103 7 0.036177328118263574 1103 8 0.06608727684435678 1103 9 0.06087133345635465 1103 10-11 0.1230144429123925 1103 12-13 0.14255830193890517 1103 14-15 0.16477036022514113 1103 16-17 0.21660271726669578 1103 18-19 0.2321564203325579 1103 20-21 0.19150328301985553 1103 22-23 0.18081362343014717 1103 24-25 0.17412607097781319 1103 26-27 0.2527720440723229 1103 28-29 0.3137629499940786 1103 30-31 0.2027902956951877 1103 32-33 0.36920987173039066 1103 34-35 0.33180792867327114 1103 36-37 0.2863995715422618 1103 38-39 0.4773604119236836 1103 40-41 0.41764724743185155 1103 42-43 0.3933898937282976 1103 44-45 0.2605047421914861 1103 46-47 0.22010314600738923 1103 48-49 0.2536600313172599 1103 50-51 0.25980932633242304 1103 52-53 0.3156140738949347 1103 54-55 0.3715409800374445 1103 56-57 0.208675381743177 1103 58-59 0.21967746373152153 1103 60-61 0.13252136883044585 1103 62-63 0.2834534854808979 1103 64-65 0.2408738382604909 1103 66-67 0.28447995623504596 1103 68-69 0.3032894674633795 1103 70-71 0.20967797671172406 1103 72-73 0.16884031096019214 1103 74-75 0.3571171262292552 1103 76-77 0.3063848893954102 1103 78-79 0.49397083014343224 1103 80-81 0.378973366634888 1103 82-83 0.3420365638501366 1103 84-85 0.4132104926279965 1103 86-87 0.3604171906586089 1103 88-89 0.2219651132450906 1103 90-91 0.35493175436757696 1103 92-93 0.392939776588193 1103 94-95 0.3864881916422789 1103 96-97 0.2569452076952672 1103 98-99 0.2240063086141646 1103 100-101 0.2886155118856095 1103 102-103 0.20923962453776568 1103 104-105 0.2294378704615525 1103 106-107 0.2923005082209045 1103 108-109 0.3067051317042342 1103 110-111 0.3549873400614558 1103 112-113 0.4241918978464483 1103 114-115 0.45166317872168804 1103 116-117 0.37695771652020227 1103 118-119 0.5494263791363849 1103 120-121 0.3559951401238166 1103 122-123 0.45684231849947565 1103 124-125 0.38523918875859664 1104 1 0.017949635015028775 1104 2 0.07814068299740029 1104 3 0.11763568104794331 1104 4 -0.04239724180227711 1104 5 -0.0019029716909670924 1104 6 0.062288846267179565 1104 7 0.02035450837809094 1104 8 0.08438810358255466 1104 9 0.1469448237485267 1104 10-11 0.1352471879260264 1104 12-13 0.10209614535536105 1104 14-15 0.09373048031741149 1104 16-17 0.11498787350807049 1104 18-19 0.14807526974257712 1104 20-21 0.06005301424715981 1104 22-23 0.033512940184564854 1104 24-25 -0.03521860930961651 1104 26-27 0.028851825963563726 1104 28-29 0.037203019753562216 1104 30-31 0.1427441573020829 1104 32-33 0.11392823988827416 1104 34-35 0.04197769146894359 1104 36-37 0.06353899116983541 1104 38-39 0.21807202489100064 1104 40-41 0.20624400008370714 1104 42-43 0.19448154135459816 1104 44-45 0.11173325969206616 1104 46-47 0.32675954743348967 1104 48-49 0.1814059831699737 1104 50-51 0.20410107992250204 1104 52-53 0.2293584583668249 1104 54-55 0.05742461856852543 1104 56-57 0.08925089748873916 1104 58-59 -0.027273318303208782 1104 60-61 0.11872124299846831 1104 62-63 0.19611615643026425 1104 64-65 0.17325570520972633 1104 66-67 0.2566534277627994 1104 68-69 0.23262763539388942 1104 70-71 0.20044477914448322 1104 72-73 0.1534645258900511 1104 74-75 0.1054890369989323 1104 76-77 0.09698501964255257 1104 78-79 0.03041795311659712 1104 80-81 0.11294082692606366 1104 82-83 0.1481394149379227 1104 84-85 0.18424871685175503 1104 86-87 0.2360642871408345 1104 88-89 0.2146304332887965 1104 90-91 0.08991194134948444 1104 92-93 -0.04805164696888653 1104 94-95 0.027248923233834432 1104 96-97 0.20468906234828665 1104 98-99 0.16704526133887043 1104 100-101 0.18433777641975269 1104 102-103 0.20622903919829838 1104 104-105 0.18574988962333805 1104 106-107 0.16708940851560783 1104 108-109 0.07838079425376066 1104 110-111 0.13067803622138285 1104 112-113 0.08964991522009313 1104 114-115 0.09832090469495824 1104 116-117 0.1399796708895522 1104 118-119 0.13298322980472932 1104 120-121 0.12356746591827061 1104 122-123 0.09895795146744746 1104 124-125 -0.05992179891207883 1105 1 0.08058089814134206 1105 2 0.10637564935117894 1105 3 0.09252959901116498 1105 4 -0.13361707807162304 1105 5 0.008480209484289958 1105 6 0.04585684592429118 1105 7 0.1119493821511881 1105 8 0.08549178537930402 1105 9 0.14749666464690137 1105 10-11 0.061008514058634944 1105 12-13 -0.0028072021222484977 1105 14-15 0.031876080591729306 1105 16-17 0.20596661423928708 1105 18-19 0.14872354885620354 1105 20-21 0.1075220468647089 1105 22-23 0.10314401043444121 1105 24-25 0.14256524050393438 1105 26-27 0.22669214570981921 1105 28-29 0.18334174076851895 1105 30-31 0.09666544228772267 1105 32-33 0.09087950644349974 1105 34-35 0.18885577213416127 1105 36-37 0.1676976213185668 1105 38-39 0.2266871721200161 1105 40-41 0.22852658548510618 1105 42-43 0.2635822014206042 1105 44-45 0.29463637143181387 1105 46-47 0.11669568390427543 1105 48-49 0.28609770234188403 1105 50-51 0.28666290753383095 1105 52-53 0.2565888599784145 1105 54-55 0.3402136117535548 1105 56-57 0.2929525176507539 1105 58-59 0.3118570233023874 1105 60-61 0.25436962926566054 1105 62-63 0.2179754852202862 1105 64-65 0.24298857135348584 1105 66-67 0.19691263225467992 1105 68-69 0.10974659729007641 1105 70-71 0.07844507917447885 1105 72-73 0.017923293195345025 1105 74-75 -0.11036647712391812 1105 76-77 -4.604391890481452E-4 1105 78-79 -0.05360116308073515 1105 80-81 0.1263591330423921 1105 82-83 -0.017750388328117594 1105 84-85 -0.04346869774684592 1105 86-87 -0.08853603003373678 1105 88-89 0.026752716945736665 1105 90-91 -0.0441827181164669 1105 92-93 0.06981996162055282 1105 94-95 0.09132140191027105 1105 96-97 0.11347726259688784 1105 98-99 0.1275511333546362 1105 100-101 0.029999128269224684 1105 102-103 0.04287609676119786 1105 104-105 0.012605789499033904 1105 106-107 0.05674801723363032 1105 108-109 -0.0489283652336141 1105 110-111 0.10057056118816377 1105 112-113 0.033935415198655505 1105 114-115 -0.12002057230988328 1105 116-117 -0.1861422270145212 1105 118-119 0.040383791289450244 1105 120-121 -0.047479103024336666 1105 122-123 -0.11637251015013561 1105 124-125 -0.07190692599621329 1106 1 0.16129693329477846 1106 2 0.17874583067539618 1106 3 0.15303446656833586 1106 4 -0.05266435409195225 1106 5 0.040078167785893015 1106 6 -0.03328212128916164 1106 7 0.04580000062937728 1106 8 0.03874574219902627 1106 9 -0.0355435518719176 1106 10-11 0.019434893542864984 1106 12-13 0.1812543781358471 1106 14-15 0.18563891838784485 1106 16-17 0.07395342140992511 1106 18-19 0.23919785268233085 1106 20-21 0.23374922697461642 1106 22-23 0.2010752986391111 1106 24-25 0.2501017214556356 1106 26-27 0.22524499935379794 1106 28-29 0.2163688364937144 1106 30-31 0.2981146468074556 1106 32-33 0.26924511864429945 1106 34-35 0.14133148580242505 1106 36-37 0.2757486431968488 1106 38-39 0.2799061401567329 1106 40-41 0.23249626873371199 1106 42-43 0.3722390952399337 1106 44-45 0.20669489531680085 1106 46-47 0.5014848250325628 1106 48-49 0.34103395072109066 1106 50-51 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-0.8023859555165558 2216 82-83 -0.8156300534321019 2216 84-85 -0.8736812514513872 2216 86-87 -0.8189759482681893 2216 88-89 -0.9209366106917471 2216 90-91 -0.795012766955459 2216 92-93 -0.6944178622897539 2216 94-95 -0.6892228653210779 2216 96-97 -0.7003385751939746 2216 98-99 -0.7586770941200527 2216 100-101 -0.7789549948931977 2216 102-103 -0.7562650895736773 2216 104-105 -0.8141396846309803 2216 106-107 -0.8226829900249015 2216 108-109 -0.7448719127323002 2216 110-111 -0.6762044427899525 2216 112-113 -0.7153133332253958 2216 114-115 -0.7164056904904825 2216 116-117 -0.6993648667687324 2216 118-119 -0.5817925126140011 2216 120-121 -0.6036646116721514 2216 122-123 -0.7794889648547212 2216 124-125 -0.9785543549103757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 0.0 4 11.0 5 26.0 6 57.0 7 138.0 8 231.0 9 359.0 10 728.0 11 1024.0 12 1336.0 13 2071.0 14 3731.0 15 4106.0 16 1885.0 17 1470.0 18 1416.0 19 1076.0 20 952.0 21 873.0 22 869.0 23 1021.0 24 1197.0 25 1539.0 26 1860.0 27 2381.0 28 2953.0 29 3663.0 30 4400.0 31 5434.0 32 6933.0 33 9239.0 34 13635.0 35 27207.0 36 65362.0 37 195207.0 38 117778.0 39 1101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.38293904970436 17.96389464338562 13.629844556154117 23.023321750755905 2 15.98140162394365 19.293849375501793 40.71550361290216 24.0092453876524 3 19.89223616172025 24.648908808369494 28.401399605610838 27.05745542429942 4 12.991310257377165 15.953986217413465 36.238132927784385 34.816570597424985 5 14.051477163580964 37.27830126188189 32.77912098830855 15.891100586228596 6 32.258091219327454 35.65144688944121 16.930355187920753 15.160106703310575 7 29.263220624111536 32.59690572112589 19.71180208533787 18.428071569424702 8 26.817221522143527 35.191473248372056 18.6902389716913 19.301066257793117 9 27.258995321543043 16.32121403992311 19.445910126448698 36.97388051208515 10-11 25.921465654414945 26.38248299636442 25.808073597238863 21.887977751981776 12-13 26.837085824130575 23.50453878608423 27.110116516546345 22.548258873238854 14-15 23.483639885035352 24.439092118631265 25.76130971964435 26.315958276689035 16-17 22.67841143521651 28.092157946031175 25.934501602593947 23.29492901615837 18-19 22.64964958129776 25.819971486449855 29.226285437604364 22.30409349464802 20-21 23.380386898665158 24.876830981689665 29.64705687604883 22.09572524359635 22-23 23.15660312159229 24.790959259558143 29.531078321218178 22.52135929763139 24-25 22.719174638252436 25.147689016075603 29.57763527900033 22.55550106667163 26-27 22.44904082320978 25.482485272482354 29.52766414431415 22.54080975999371 28-29 22.42554477039048 25.426411767992267 29.624314833935816 22.523728627681432 30-31 22.686815159374977 25.049117641277235 29.5371095548426 22.726957644505184 32-33 22.422555087227046 25.188348759205347 29.557150217576183 22.831945935991424 34-35 22.866723294014918 25.291111816941232 29.27837376739817 22.563791121645682 36-37 22.925137551653 25.210491792423745 29.115634434367326 22.74873622155593 38-39 22.718337313198113 25.22203093120559 29.230278976795386 22.82935277880091 40-41 22.90322981135121 25.04164261223848 29.223388560231257 22.83173901617906 42-43 22.879886441702048 25.072525844445 28.93201814284177 23.115569571011182 44-45 22.862791797242366 25.333737518791004 28.86970502393557 22.933765660031057 46-47 23.057615846763305 25.886419528284417 28.16357441930481 22.892390205647466 48-49 23.28607536788479 25.78881969584259 27.867473531849207 23.05763140442341 50-51 23.266583552757503 26.067798188392675 27.6723794546555 22.99323880419432 52-53 23.475156172728383 25.835283552564874 27.624312250273654 23.065248024433092 54-55 23.161776116901418 25.871391059822585 27.604447948286598 23.362384874989395 56-57 23.128924424469474 26.229148625565546 27.618533267531813 23.023393682433166 58-59 23.240345190416527 25.81613364799558 27.68507013167601 23.258451029911882 60-61 23.206472538785054 25.831389123113773 27.917996575431047 23.04414176267013 62-63 23.086067350399407 25.993914482022273 28.00497021903519 22.915047948543126 64-65 22.901261710525635 25.718305924711217 27.925445788866533 23.454986575896623 66-67 22.986140424933165 26.068954386313408 27.69504382588831 23.24986136286511 68-69 22.835182525854897 25.848897312911518 28.128039166187296 23.18788099504629 70-71 22.94245452852443 25.79469033873404 27.875825108117613 23.387030024623918 72-73 22.9863081160158 26.02109754406875 27.626381448397304 23.366212891518153 74-75 22.94929321291967 26.346609667096715 27.406247122518383 23.29784999746523 76-77 23.21847048416217 26.03963725343114 27.42088844973953 23.32100381266716 78-79 23.174486575966068 26.1706067973721 27.486731157208627 23.16817546945321 80-81 23.133428102607397 26.408244513003876 27.25258080735972 23.205746577029004 82-83 23.200573581719876 26.345547809857244 27.30337962129536 23.15049898712752 84-85 23.25738992067688 26.22763573233956 27.168918828041505 23.346055518942055 86-87 23.14110807743361 26.212939330013647 27.347378374994957 23.298574217557785 88-89 23.11646067799346 26.24995085654456 27.180158873031935 23.453429592430044 90-91 23.101586352382736 26.32208964396306 27.386589720006167 23.189734283648043 92-93 23.139365497148628 26.130409952077073 27.290306160454897 23.439918390319402 94-95 23.006410442109445 26.092651373754848 27.33076888059362 23.57016930354209 96-97 23.19396867010914 26.01843871986327 27.231305474195416 23.55628713583218 98-99 22.992205330668206 25.981575859907007 27.144672125211315 23.88154668421347 100-101 22.749949304541072 26.069984563750058 27.383928091078012 23.79613804063086 102-103 22.942648035606762 25.801659083055544 27.38759598492796 23.868096896409735 104-105 22.99893022457007 25.836214691720517 27.336383331367596 23.828471752341816 106-107 23.028153567925706 25.896275021830085 27.191285347150085 23.88428606309412 108-109 23.058411635563044 25.760588647918276 27.101538041330336 24.079461675188348 110-111 22.866501544656398 25.94226109555764 27.367214383409998 23.824022976375964 112-113 22.877435841726545 26.02645478012115 27.325708314581163 23.770401063571136 114-115 22.684133801119252 26.159523826767465 27.11675369540184 24.03958867671144 116-117 22.6538485811923 26.047771923054242 27.341237037673128 23.95714245808033 118-119 22.674296497775092 26.065405351158805 27.159494990983458 24.10080316008264 120-121 22.637130932649672 26.132523863027362 27.243372875709476 23.986972328613493 122-123 22.637775113107658 26.210870129749203 27.110661864742585 24.04069289240055 124-125 22.680484900403393 26.214501282397478 27.045395525731237 24.059618291467892 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 89.0 1 82.5 2 259.0 3 400.0 4 210.0 5 95.5 6 281.5 7 741.5 8 1345.5 9 1484.5 10 1003.0 11 563.5 12 439.0 13 460.5 14 529.5 15 572.5 16 608.0 17 686.5 18 742.0 19 786.5 20 809.0 21 929.0 22 1067.5 23 1257.5 24 1572.5 25 1847.5 26 2210.0 27 2667.5 28 3114.0 29 3631.5 30 4452.5 31 5337.5 32 6099.5 33 6714.5 34 7473.0 35 8093.0 36 9027.0 37 10114.0 38 10614.5 39 11115.0 40 11555.0 41 12412.0 42 13389.5 43 14331.0 44 15582.0 45 16465.5 46 16700.0 47 17140.5 48 18020.0 49 18315.0 50 18216.5 51 18165.5 52 18197.5 53 18564.5 54 18776.0 55 18049.0 56 16411.0 57 15052.5 58 14325.5 59 12942.5 60 11129.0 61 9173.5 62 7231.5 63 5734.0 64 4419.5 65 3336.5 66 2465.5 67 2004.0 68 1578.5 69 1053.0 70 751.0 71 558.0 72 436.0 73 390.5 74 312.5 75 228.5 76 159.0 77 90.0 78 52.0 79 35.5 80 19.5 81 12.0 82 7.5 83 3.5 84 2.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01696742461393936 2 0.0022761179360162556 3 0.0 4 0.13263559972603817 5 0.0395216841617368 6 0.014484386865557992 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.1383962473022826E-4 30-31 1.0345990618255707E-4 32-33 0.0 34-35 5.172995309127853E-4 36-37 0.001241518874190685 38-39 0.002689957560746484 40-41 0.0 42-43 6.207594370953425E-4 44-45 5.172995309127853E-4 46-47 1.0345990618255707E-4 48-49 0.0019657382174685842 50-51 0.001344978780373242 52-53 0.0 54-55 0.0 56-57 0.0015518985927383561 58-59 0.0019657382174685842 60-61 0.001344978780373242 62-63 1.0345990618255707E-4 64-65 0.001344978780373242 66-67 6.207594370953425E-4 68-69 0.0010345990618255707 70-71 0.0018622783112860273 72-73 0.0 74-75 1.0345990618255707E-4 76-77 0.004448775965849954 78-79 8.276792494604565E-4 80-81 0.0 82-83 0.0 84-85 5.172995309127853E-4 86-87 1.0345990618255707E-4 88-89 0.0 90-91 1.0345990618255707E-4 92-93 6.207594370953425E-4 94-95 0.0010345990618255707 96-97 5.172995309127853E-4 98-99 0.0 100-101 2.0691981236511413E-4 102-103 0.0 104-105 0.0 106-107 2.0691981236511413E-4 108-109 4.1383962473022826E-4 110-111 0.0 112-113 3.1037971854767124E-4 114-115 0.0019657382174685842 116-117 7.242193432778995E-4 118-119 1.0345990618255707E-4 120-121 0.0 122-123 1.0345990618255707E-4 124-125 9.311391556430137E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 483279.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.48952191120271 #Duplication Level Percentage of deduplicated Percentage of total 1 81.17894449211693 51.54012375060566 2 11.005238183339193 13.974346215582367 3 3.1564317429832305 6.012010269220486 4 1.4311002369873536 3.6343947941334394 5 0.8221146526313721 2.6097833125880148 6 0.5160742064354235 1.9659182782373017 7 0.3555611432690994 1.580208489744904 8 0.2648684462040383 1.3453096815086005 9 0.1916049035092006 1.0948413347677144 >10 1.0246083305050233 11.372622459322391 >50 0.03291963360054918 1.4003346987190213 >100 0.019230280582552675 2.019072766641903 >500 3.25936959026316E-4 0.1179534157001008 >1k 9.77810877078948E-4 1.3330805332281568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2964 0.6133103238501982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2138 0.4423945588366141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.277272548569253 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 570 0.11794429304811506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 2.0691981236511416E-4 0.0 28-29 0.0 0.0 0.0 7.242193432778995E-4 0.0 30-31 0.0 0.0 0.0 0.0024830377483813695 0.0 32-33 0.0 0.0 0.0 0.006517974089501095 0.0 34-35 0.0 0.0 0.0 0.010139070805890594 0.0 36-37 0.0 0.0 0.0 0.014691306677923104 0.0 38-39 0.0 0.0 0.0 0.024209618046718354 0.0 40-41 0.0 0.0 0.0 0.03972860397410192 0.0 42-43 0.0 0.0 0.0 0.05224725262219132 0.0 44-45 0.0 0.0 0.0 0.06869737770521789 0.0 46-47 0.0 0.0 0.0 0.08804438016135607 0.0 48-49 0.0 0.0 0.0 0.1111159392400663 0.0 50-51 0.0 0.0 0.0 0.13563593700533233 0.0 52-53 0.0 0.0 0.0 0.15777635692839953 0.0 54-55 0.0 0.0 0.0 0.18674513065951553 0.0 56-57 0.0 0.0 0.0 0.22181803885540236 0.0 58-59 0.0 0.0 0.0 0.2576151663945671 0.0 60-61 0.0 0.0 0.0 0.3059309425818213 0.0 62-63 0.0 0.0 0.0 0.3493841031784952 0.0 64-65 0.0 0.0 0.0 0.4004932968326784 0.0 66-67 0.0 0.0 0.0 0.455016667390886 0.0 68-69 0.0 0.0 0.0 0.522989825752826 0.0 70-71 0.0 0.0 0.0 0.5981017176413624 0.0 72-73 0.0 0.0 0.0 0.6734205293422639 0.0 74-75 0.0 0.0 0.0 0.7456355438576888 0.0 76-77 0.0 0.0 0.0 0.8284034688037345 0.0 78-79 0.0 0.0 0.0 0.9330014339542997 0.0 80-81 0.0 0.0 0.0 1.0509457270024147 0.0 82-83 0.0 0.0 0.0 1.176339133295674 0.0 84-85 0.0 0.0 0.0 1.3047328768682274 0.0 86-87 0.0 0.0 0.0 1.4392307549055514 0.0 88-89 0.0 0.0 0.0 1.6073531024522065 0.0 90-91 0.0 0.0 0.0 1.7906840562076978 0.0 92-93 0.0 0.0 0.0 1.967910875498418 0.0 94-95 0.0 0.0 0.0 2.1615878198721648 0.0 96-97 0.0 0.0 0.0 2.3475052712822198 0.0 98-99 0.0 0.0 0.0 2.5616672770801134 0.0 100-101 0.0 0.0 0.0 2.821972401035427 0.0 102-103 0.0 0.0 0.0 3.0929338953275436 0.0 104-105 0.0 0.0 0.0 3.364826528775304 0.0 106-107 0.0 0.0 0.0 3.6318565466324833 0.0 108-109 0.0 0.0 0.0 3.920716604694183 0.0 110-111 0.0 0.0 0.0 4.24547725020123 0.0 112-113 0.0 0.0 0.0 4.590102197695327 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1080 0.0 73.94682 1 GTATCAA 2055 0.0 57.133774 1 CCCGCTA 35 0.00779659 50.99494 9 ATCAACG 2620 0.0 43.89848 3 TCAACGC 2685 0.0 42.83576 4 CAACGCA 2740 0.0 42.145363 5 GTGGTAT 450 0.0 41.05704 1 TATCAAC 2810 0.0 40.930256 2 AACGCAG 2850 0.0 40.50193 6 TCTAGCG 30 0.0013087136 39.664787 118-119 TGGTATC 475 0.0 37.63753 2 ACGCAGA 3350 0.0 34.630898 7 CGCAGAG 3365 0.0 34.29972 8 GTATAAC 140 7.932431E-8 34.05653 1 GTACATG 2595 0.0 33.531574 1 TACATGG 2625 0.0 32.463863 2 TAACGCA 75 0.0038785671 31.782803 4 TATAACG 75 0.0038785671 31.782803 2 ATAACGC 95 3.2536566E-4 31.364609 3 GCAGAGT 3650 0.0 31.295528 9 >>END_MODULE