FastQCFastQC Report
Fri 27 May 2016
SRR940077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756746
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.053.014141
GTATCAA14750.050.1387981
ATCAACG14700.047.852813
TCAACGC14750.047.693764
CAACGCA15200.046.2541925
AACGCAG15800.044.4977076
TATCAAC17050.041.6344832
ACGCAGA19500.035.443467
GTACATG33450.033.698451
CGCAGAG20500.033.4238668
TACATGG34450.032.20732
ACATGGG35850.029.4324823
GCAGAGT24650.027.300659
CATGGGG24450.027.0654774
GTAGGAC2308.101779E-925.9188043
GTGGTAT5250.024.9923781
GGACCGA1751.7312435E-523.8326686
CAGAGTA24250.022.57130210-11
GTGTAGC5650.022.1674181
ATGGGGA13900.021.4322555
Produced by FastQC (version 0.11.5)