##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 756746 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.479850570733113 34.0 31.0 34.0 30.0 34.0 2 31.608040214285904 34.0 31.0 34.0 29.0 34.0 3 31.697944885073724 34.0 31.0 34.0 30.0 34.0 4 34.892065501502486 37.0 35.0 37.0 33.0 37.0 5 34.979998572836855 37.0 35.0 37.0 33.0 37.0 6 34.921524527384356 37.0 37.0 37.0 32.0 37.0 7 34.94588540937118 37.0 37.0 37.0 33.0 37.0 8 34.89847980696297 37.0 37.0 37.0 32.0 37.0 9 36.527916896818745 39.0 39.0 39.0 33.0 39.0 10-11 36.5430732372553 39.0 38.5 39.0 33.0 39.0 12-13 36.51088806548036 39.0 39.0 39.0 33.0 39.0 14-15 37.990270843849856 41.0 39.0 41.0 33.0 41.0 16-17 38.0202809132787 41.0 39.0 41.0 33.0 41.0 18-19 38.03371408636451 41.0 39.5 41.0 33.0 41.0 20-21 38.05402275003766 41.0 39.0 41.0 33.0 41.0 22-23 38.05780737526198 41.0 39.0 41.0 33.0 41.0 24-25 38.10887602973785 41.0 39.0 41.0 33.0 41.0 26-27 38.141201935656085 41.0 39.0 41.0 33.0 41.0 28-29 38.0933800773311 41.0 39.0 41.0 33.0 41.0 30-31 38.03486308483956 41.0 39.0 41.0 33.0 41.0 32-33 37.98755526953562 41.0 39.0 41.0 33.0 41.0 34-35 37.90374445322473 41.0 39.0 41.0 32.0 41.0 36-37 37.77139291122781 41.0 38.5 41.0 32.0 41.0 38-39 37.66513731159464 41.0 38.0 41.0 31.0 41.0 40-41 37.56904033850195 41.0 38.0 41.0 31.0 41.0 42-43 37.397867976837674 40.0 38.0 41.0 31.0 41.0 44-45 37.22493941163878 40.0 38.0 41.0 30.0 41.0 46-47 37.05665639461589 40.0 37.5 41.0 30.0 41.0 48-49 36.90255911494742 40.0 37.0 41.0 30.0 41.0 50-51 36.72880398442806 40.0 37.0 41.0 30.0 41.0 52-53 36.86381626067399 40.0 37.0 41.0 30.0 41.0 54-55 36.67413042156814 40.0 36.5 41.0 30.0 41.0 56-57 36.55449384601967 40.0 36.0 41.0 30.0 41.0 58-59 36.364256963366834 40.0 35.5 41.0 30.0 41.0 60-61 36.119795809954724 39.0 35.0 41.0 30.0 41.0 62-63 35.83826211172573 39.0 35.0 41.0 30.0 41.0 64-65 35.52519471526774 38.5 35.0 41.0 29.0 41.0 66-67 35.20691751261322 37.5 35.0 40.0 29.0 41.0 68-69 34.85518985234147 37.0 35.0 40.0 29.0 41.0 70-71 34.48675975822799 36.5 35.0 39.0 28.5 41.0 72-73 34.113322039363275 36.0 34.0 39.0 28.0 41.0 74-75 33.7412910012078 35.5 34.0 38.5 28.0 40.5 76-77 33.37867857907409 35.0 34.0 37.0 27.0 39.5 78-79 33.031707468556164 35.0 34.0 37.0 27.0 39.0 80-81 32.71542565140747 35.0 34.0 36.5 26.0 39.0 82-83 32.41337317937591 35.0 34.0 36.0 26.0 37.0 84-85 32.172544684742306 35.0 34.0 36.0 26.0 37.0 86-87 31.959635069098482 35.0 33.0 35.0 25.5 37.0 88-89 31.793149220478206 35.0 33.0 35.0 25.0 36.0 90-91 31.63434296316069 35.0 33.0 35.0 25.0 36.0 92-93 31.48780105874362 35.0 33.0 35.0 25.0 36.0 94-95 31.378062123883048 35.0 33.0 35.0 24.0 35.5 96-97 31.270940976232446 35.0 33.0 35.0 24.0 35.0 98-99 31.20084215839925 35.0 33.0 35.0 24.0 35.0 100-101 31.104205374062104 35.0 33.0 35.0 23.0 35.0 102-103 30.383379628039 34.0 31.0 35.0 22.0 35.0 104-105 31.320336678357073 35.0 33.0 35.0 24.5 35.0 106-107 31.443987150245924 35.0 34.0 35.0 25.0 35.0 108-109 31.43190581780412 35.0 34.0 35.0 25.0 35.0 110-111 31.38380843770565 35.0 34.0 35.0 24.0 35.0 112-113 31.32935886017237 35.0 34.0 35.0 24.0 35.0 114-115 31.269373739669586 35.0 34.0 35.0 24.0 35.0 116-117 31.196891030808224 35.0 33.0 35.0 23.5 35.0 118-119 31.11461573632368 35.0 33.0 35.0 23.0 35.0 120-121 31.019097557172422 35.0 33.0 35.0 20.5 35.0 122-123 30.882192968314335 35.0 33.0 35.0 18.5 35.0 124-125 29.62725868389129 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1435450213529954 1101 2 0.416427051205158 1101 3 0.4485816836705041 1101 4 0.3663111214880459 1101 5 0.40673586319473287 1101 6 0.5611770186620717 1101 7 0.5443382590481178 1101 8 0.505084621380611 1101 9 0.5396928801372951 1101 10-11 0.5234324364723406 1101 12-13 0.45457265611119624 1101 14-15 0.5685558716761889 1101 16-17 0.47390832329671184 1101 18-19 0.5282378909043572 1101 20-21 0.40102088878424524 1101 22-23 0.42885793786395965 1101 24-25 0.3481796353863231 1101 26-27 0.36738179773259105 1101 28-29 0.37684101731737485 1101 30-31 0.46044397056556363 1101 32-33 0.5575399782118708 1101 34-35 0.5357542877818773 1101 36-37 0.4760738067210255 1101 38-39 0.44772303521155266 1101 40-41 0.4985791344368451 1101 42-43 0.6377109511863779 1101 44-45 0.6353849728392547 1101 46-47 0.5294752626988526 1101 48-49 0.5115625155496772 1101 50-51 0.5976946409830717 1101 52-53 0.5672470861505587 1101 54-55 0.5371814654249576 1101 56-57 0.4593681187112395 1101 58-59 0.3704724538931927 1101 60-61 0.3931104381389616 1101 62-63 0.3384381493530455 1101 64-65 0.250534446951896 1101 66-67 0.26252085182671436 1101 68-69 0.2666217273662852 1101 70-71 0.2537775507302058 1101 72-73 0.02753112341929409 1101 74-75 0.194048161324055 1101 76-77 0.032720440665087835 1101 78-79 0.09842535652386886 1101 80-81 0.17143383181532101 1101 82-83 0.1335092530371682 1101 84-85 0.07873471112284847 1101 86-87 0.017640462360873244 1101 88-89 -0.06310255323101543 1101 90-91 -0.018717075130449956 1101 92-93 0.20045087575413234 1101 94-95 0.1989606133402937 1101 96-97 0.2362201174439953 1101 98-99 0.34019323260726253 1101 100-101 0.28257685021523926 1101 102-103 0.32009370880212984 1101 104-105 0.35802795397808396 1101 106-107 0.39744966934402726 1101 108-109 0.5223944033182448 1101 110-111 0.43964293790346787 1101 112-113 0.37446510865742866 1101 114-115 0.38260036722346413 1101 116-117 0.4072012323482994 1101 118-119 0.2881723971110972 1101 120-121 0.3557442798158128 1101 122-123 0.4027756821165056 1101 124-125 0.279673301333915 1102 1 0.18954841004741496 1102 2 0.3818116307836199 1102 3 0.36197912911624996 1102 4 0.20832953587696323 1102 5 0.31207352238889285 1102 6 0.2842071072894612 1102 7 0.39556386789596587 1102 8 0.38018702698126816 1102 9 0.48766385280431024 1102 10-11 0.43748039022224816 1102 12-13 0.3575107658899981 1102 14-15 0.5283640008189607 1102 16-17 0.4489527766369861 1102 18-19 0.4358086904128058 1102 20-21 0.46129240326997945 1102 22-23 0.4464856655767804 1102 24-25 0.34648179707545523 1102 26-27 0.3440356481831728 1102 28-29 0.43115322406497825 1102 30-31 0.4969038554988998 1102 32-33 0.48561168075153205 1102 34-35 0.5346092069745509 1102 36-37 0.5621422229718576 1102 38-39 0.5766272487365356 1102 40-41 0.4141526988965154 1102 42-43 0.43537028499139296 1102 44-45 0.40597082597100353 1102 46-47 0.5140446006001014 1102 48-49 0.3526371692325583 1102 50-51 0.36571220819838857 1102 52-53 0.4530264393195438 1102 54-55 0.6222409537693352 1102 56-57 0.47373166790316645 1102 58-59 0.4641258667925783 1102 60-61 0.5001882943242606 1102 62-63 0.5210959936220476 1102 64-65 0.435169417533551 1102 66-67 0.5038326675711104 1102 68-69 0.4413000806842291 1102 70-71 0.3301348904188899 1102 72-73 0.4527284869405577 1102 74-75 0.4133416092275226 1102 76-77 0.43417134183437156 1102 78-79 0.3054359508487394 1102 80-81 0.25666787516082223 1102 82-83 0.3369286691390485 1102 84-85 0.25962838618056594 1102 86-87 0.3381560280121647 1102 88-89 0.5257040491373424 1102 90-91 0.1840378682671826 1102 92-93 0.23656397808525753 1102 94-95 0.3290366727727765 1102 96-97 0.3292428049393443 1102 98-99 0.20632002005047312 1102 100-101 0.3802008075400316 1102 102-103 0.30605948671231076 1102 104-105 0.3876778664678433 1102 106-107 0.26184952597618505 1102 108-109 0.2755841050386607 1102 110-111 0.3267477453809384 1102 112-113 0.34318950725726793 1102 114-115 0.3034815674171014 1102 116-117 0.4246101844597163 1102 118-119 0.25915088262535946 1102 120-121 0.2605024164062648 1102 122-123 0.2732430705874975 1102 124-125 0.12508390124454394 1103 1 -0.09671953430658675 1103 2 0.0952153232506916 1103 3 0.18667019023805054 1103 4 0.038177286357949924 1103 5 0.06710071640991089 1103 6 0.14477762675076633 1103 7 0.09885767431893555 1103 8 0.05903858832505904 1103 9 -0.08675434314063324 1103 10-11 0.006489843381935145 1103 12-13 0.019786923948302615 1103 14-15 0.1067595535790602 1103 16-17 0.1826248019440797 1103 18-19 0.11050946368496994 1103 20-21 -0.07344703361029303 1103 22-23 -0.00561030896813719 1103 24-25 0.1193955346809048 1103 26-27 0.12221475873519694 1103 28-29 0.2525976520655675 1103 30-31 0.18362457105314434 1103 32-33 0.2981533042530842 1103 34-35 0.14606769446900358 1103 36-37 0.20312795998532351 1103 38-39 0.20402060007588574 1103 40-41 0.20776025402453513 1103 42-43 0.24734999122197365 1103 44-45 0.21833670579933084 1103 46-47 0.16471242655835994 1103 48-49 0.257862768610984 1103 50-51 0.07640806064769379 1103 52-53 0.15953219261243134 1103 54-55 0.23600723318327965 1103 56-57 0.14229528711287287 1103 58-59 0.09121397912053197 1103 60-61 0.0407550214220862 1103 62-63 -5.967879378161456E-4 1103 64-65 -0.2172512203234831 1103 66-67 -0.27255200758057896 1103 68-69 -0.21803005843076306 1103 70-71 -0.03959279938427329 1103 72-73 -0.13314542159874065 1103 74-75 -0.18844866382264058 1103 76-77 -0.2069369532670038 1103 78-79 -0.2059550140765296 1103 80-81 -0.20057091424276052 1103 82-83 -0.1408258816971184 1103 84-85 -0.16149276595795214 1103 86-87 -0.13599115268932493 1103 88-89 -0.08591630752981061 1103 90-91 -0.04020874635468985 1103 92-93 -0.13427135934259482 1103 94-95 -0.27192806749657095 1103 96-97 -0.05296962291325613 1103 98-99 -0.18148383151087089 1103 100-101 -0.17688120000769558 1103 102-103 -0.12794413915715808 1103 104-105 -0.005657285966670145 1103 106-107 -0.06715419238995324 1103 108-109 -0.08449386965425276 1103 110-111 -0.006494632893442542 1103 112-113 -0.11166286805722336 1103 114-115 -0.10427381588909768 1103 116-117 -0.005388335593561777 1103 118-119 -0.027595731996512995 1103 120-121 -0.04409836796549982 1103 122-123 -0.0233174407340897 1103 124-125 -0.05764095816051551 1104 1 -0.11609095776867662 1104 2 -0.0795030243887922 1104 3 -0.08917029617110828 1104 4 -0.05485293538740166 1104 5 -0.08325693738122197 1104 6 -0.014320191561111528 1104 7 0.08177076444768971 1104 8 0.0078091534180444455 1104 9 0.12218253096523313 1104 10-11 0.020849643095807835 1104 12-13 -0.015998573190458387 1104 14-15 0.009631960589068456 1104 16-17 0.084638839855387 1104 18-19 0.04213052949326368 1104 20-21 0.02950361016653602 1104 22-23 0.021144429944591536 1104 24-25 0.05413265914442178 1104 26-27 0.015497573470533155 1104 28-29 0.06885078153624136 1104 30-31 -0.02008608702983139 1104 32-33 -0.010940043386405307 1104 34-35 0.11589512651477918 1104 36-37 0.17588144710978781 1104 38-39 -0.013117722527830722 1104 40-41 0.04606721039076689 1104 42-43 0.028204336715532463 1104 44-45 0.08722082597100211 1104 46-47 -0.022539451124039545 1104 48-49 -0.1933398422617003 1104 50-51 0.016699995554702696 1104 52-53 -0.1931122101057383 1104 54-55 -0.08375760944905153 1104 56-57 -0.06500755623476806 1104 58-59 -0.03371896079362813 1104 60-61 0.0329648747840352 1104 62-63 0.03438622350710574 1104 64-65 0.09668163017723685 1104 66-67 0.1989116905596191 1104 68-69 0.0022735002244544944 1104 70-71 0.07785399961429817 1104 72-73 -0.0741213693812881 1104 74-75 -0.18035451146212722 1104 76-77 -0.03989475011965027 1104 78-79 -0.10959637673746414 1104 80-81 -0.1284075558736646 1104 82-83 -0.04915250902187296 1104 84-85 -0.1287875620952903 1104 86-87 -0.04438707543610576 1104 88-89 -0.004234887644262386 1104 90-91 0.02936832803729672 1104 92-93 0.06734702406226845 1104 94-95 -0.08949780998584345 1104 96-97 -0.07364871804916362 1104 98-99 -0.03739406040929438 1104 100-101 -0.1318861177473245 1104 102-103 -0.04334784087389565 1104 104-105 -0.02503765077353748 1104 106-107 -0.040287686667493716 1104 108-109 -0.022063165076282587 1104 110-111 0.037523607449909235 1104 112-113 0.05581522564807173 1104 114-115 -0.05456440959439135 1104 116-117 -0.06452415462074157 1104 118-119 -0.10385199093785857 1104 120-121 -0.018772008881093427 1104 122-123 -0.06858523401020022 1104 124-125 -0.0024125068014342332 1105 1 0.26416850030903305 1105 2 0.4182666586377941 1105 3 0.4459779655875806 1105 4 0.48207830183141454 1105 5 0.34332792969444625 1105 6 0.3947864035309152 1105 7 0.34839908755607496 1105 8 0.3920691227432016 1105 9 0.5301260381226953 1105 10-11 0.4561001543432539 1105 12-13 0.5906572294680217 1105 14-15 0.6623703297097236 1105 16-17 0.5709150412868738 1105 18-19 0.5730929528061495 1105 20-21 0.44164573695589837 1105 22-23 0.45372686859489875 1105 24-25 0.5460677307190593 1105 26-27 0.547038345454169 1105 28-29 0.6559258070986118 1105 30-31 0.61556064098653 1105 32-33 0.6572475844254555 1105 34-35 0.7304081633245971 1105 36-37 0.62705412093392 1105 38-39 0.6365787243024386 1105 40-41 0.6314615099817757 1105 42-43 0.6626847815008148 1105 44-45 0.6867095785272426 1105 46-47 0.7111613413094915 1105 48-49 0.6009789205194878 1105 50-51 0.5048805937142191 1105 52-53 0.6844088788513147 1105 54-55 0.7471153455611699 1105 56-57 0.7068770296548053 1105 58-59 0.5935496997380696 1105 60-61 0.6820414340887382 1105 62-63 0.6233752316870707 1105 64-65 0.5101178520586203 1105 66-67 0.6110602079420318 1105 68-69 0.6103224521672033 1105 70-71 0.684605661168483 1105 72-73 0.4781057522956047 1105 74-75 0.5551036940593477 1105 76-77 0.47158275499281643 1105 78-79 0.6298687306244943 1105 80-81 0.4679849543512873 1105 82-83 0.4914884674607478 1105 84-85 0.3809868499701423 1105 86-87 0.41273204521829143 1105 88-89 0.4223020499835535 1105 90-91 0.46192839961193854 1105 92-93 0.47942524492116334 1105 94-95 0.4530251961491274 1105 96-97 0.3281949680449081 1105 98-99 0.2593211747645263 1105 100-101 0.41138139247762595 1105 102-103 0.31281588611996725 1105 104-105 0.523562809349162 1105 106-107 0.5444228194674707 1105 108-109 0.4932135476026538 1105 110-111 0.5245762659366129 1105 112-113 0.500111621353593 1105 114-115 0.37341999735857456 1105 116-117 0.467061351514225 1105 118-119 0.3717806777737991 1105 120-121 0.45032947373649534 1105 122-123 0.40189342140902085 1105 124-125 0.351316404241512 1106 1 0.002301959743157056 1106 2 0.22505453139213927 1106 3 0.09765357130691754 1106 4 -0.04059287791613997 1106 5 0.1385821430785512 1106 6 0.10393327361806115 1106 7 0.17427837093855914 1106 8 0.22400041440519658 1106 9 0.31708829499498137 1106 10-11 0.36531380807214475 1106 12-13 0.40822070503807595 1106 14-15 0.43119107992645667 1106 16-17 0.3179626650751288 1106 18-19 0.3114742583641217 1106 20-21 0.16162835661683772 1106 22-23 0.250618525617341 1106 24-25 0.28959158409371355 1106 26-27 0.36945400518114724 1106 28-29 0.3195913579392169 1106 30-31 0.3068366648295324 1106 32-33 0.3131345002241446 1106 34-35 0.4141731014978731 1106 36-37 0.4843341600915352 1106 38-39 0.481387644274065 1106 40-41 0.4616298794238958 1106 42-43 0.3571185101435219 1106 44-45 0.37636889899331294 1106 46-47 0.45585494542768146 1106 48-49 0.46442089134209397 1106 50-51 0.47863690393874947 1106 52-53 0.31886695656092456 1106 54-55 0.5139118462642642 1106 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2216 36-37 -0.9988426004389339 2216 38-39 -0.8393633540497802 2216 40-41 -0.8622332055375637 2216 42-43 -0.9195040581456624 2216 44-45 -0.8068724994867651 2216 46-47 -0.8066661862096822 2216 48-49 -0.7305365099128025 2216 50-51 -0.7478541120452178 2216 52-53 -0.9267030375324055 2216 54-55 -0.7543712883228508 2216 56-57 -0.8049172948644241 2216 58-59 -0.8351531752059103 2216 60-61 -0.7634317461059084 2216 62-63 -0.7715825941735304 2216 64-65 -0.906364306522903 2216 66-67 -0.8535574224547489 2216 68-69 -0.9039749615790456 2216 70-71 -1.0342254574638687 2216 72-73 -0.988629946364938 2216 74-75 -0.9854222536357824 2216 76-77 -0.8424666146599193 2216 78-79 -0.8996039446642214 2216 80-81 -1.0188950411069762 2216 82-83 -1.0799998091793839 2216 84-85 -0.9223649764485131 2216 86-87 -0.9499356578342173 2216 88-89 -0.8810564199340831 2216 90-91 -0.9497263510333873 2216 92-93 -1.051634320690635 2216 94-95 -0.9194631082729074 2216 96-97 -0.8645429024358542 2216 98-99 -0.9591983033705098 2216 100-101 -1.061224139503576 2216 102-103 -1.0817735275248097 2216 104-105 -1.014654824429769 2216 106-107 -1.041099546777751 2216 108-109 -0.9314292435366127 2216 110-111 -0.872255630589077 2216 112-113 -1.0040707632276913 2216 114-115 -0.89822150252607 2216 116-117 -0.8911524894307448 2216 118-119 -0.9607400348441395 2216 120-121 -1.0850036735788677 2216 122-123 -1.076758902547315 2216 124-125 -0.9035886118417942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15093.0 3 2240.0 4 1382.0 5 1116.0 6 1116.0 7 1331.0 8 1467.0 9 1704.0 10 1794.0 11 2060.0 12 2505.0 13 2548.0 14 2526.0 15 2542.0 16 2348.0 17 2285.0 18 1937.0 19 1713.0 20 1636.0 21 1614.0 22 1775.0 23 2158.0 24 3513.0 25 6021.0 26 7196.0 27 6024.0 28 6528.0 29 7781.0 30 8542.0 31 10407.0 32 13312.0 33 18056.0 34 27044.0 35 48190.0 36 116821.0 37 275442.0 38 146291.0 39 688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58380402360855 18.554055340483593 13.90202238158702 23.960118254320832 2 15.361710184340522 19.251754631021058 41.245248494942985 24.14128668969544 3 19.715295905891683 24.056042031523642 27.873641079866502 28.35502098271817 4 13.017239554671296 16.449011556533886 36.77573233712907 33.75801655166575 5 14.59386300674604 37.44514877238988 33.3069871211961 14.654001099667985 6 32.44719101123595 35.912095175148714 17.500066093853274 14.140647719762061 7 29.653909709828806 31.939586225130544 19.87692511071452 18.52957895432613 8 26.07914644486509 36.436412602795535 19.061999415336956 18.422441537002424 9 26.018323135439058 18.17231685133124 19.487349098726643 36.32201091450306 10-11 25.449052911994023 27.204941846053167 26.356313975125513 20.989691266827297 12-13 25.65933659212339 23.938491879902397 26.986535680578648 23.41563584739557 14-15 22.773941033152248 25.594019920821577 24.688906495842506 26.943132550183673 16-17 23.19788187878832 28.772027687323327 25.28064379041122 22.749446643477135 18-19 23.113855819515592 27.796915376118257 26.55855741510872 22.530671389257428 20-21 23.903192428153318 26.912640024150257 26.530658180535077 22.653509367161345 22-23 23.748571307623926 26.631156605850233 26.420292194048173 23.19997989247767 24-25 23.363436673882827 26.990715087684965 26.379743788789074 23.266104449643137 26-27 23.229709985468546 27.215319946420628 26.301470525068577 23.253499543042246 28-29 23.17430467637023 27.31842822367708 26.351067400733186 23.156199699219503 30-31 23.405138235449595 27.0056034254903 26.31085795845007 23.278400380610034 32-33 23.13824708487319 27.002000133474386 26.383590844185832 23.476161937466593 34-35 23.547719281784055 27.17930487173913 26.21357362849974 23.059402217977073 36-37 23.685349455704777 26.907312883030215 26.108264367246477 23.299073294018534 38-39 23.269424556696876 27.052277885889296 26.321715658255997 23.35658189915783 40-41 23.506635011903573 26.7931844026507 26.33825180337661 23.361928782069114 42-43 23.347176930421885 26.791952872298598 26.28803272243092 23.572837474848598 44-45 23.405987438339825 26.92615977295179 26.33673310932477 23.33111967938361 46-47 23.380075995374195 27.19649760449364 26.06330745085082 23.360118949281347 48-49 23.442931270106023 26.950069652425146 26.28044154615167 23.326557531317157 50-51 23.3866486048932 27.096823220392185 26.306576578064856 23.209951596649763 52-53 23.494920952952402 26.950404288444407 26.25383604841417 23.30083871018902 54-55 23.479496881612285 26.84542603427517 26.307588407183857 23.367488676928687 56-57 23.319270810617205 27.142849590481894 26.33220595251784 23.20567364638306 58-59 23.438662668134615 26.89387280914499 26.25464075991494 23.412823762805456 60-61 23.485481236354794 26.93154005148835 26.43311007617527 23.149868635981584 62-63 23.277343855839376 27.07346875927256 26.430328819502495 23.218858565385563 64-65 23.211619590081103 26.81637058328613 26.441626113410766 23.530383713222 66-67 23.214944253150836 27.07618185302923 26.25137962712066 23.45749426669927 68-69 23.190128091580853 26.920712765535566 26.517204457428385 23.371954685455197 70-71 23.20621816172719 26.74051427649962 26.526717052757355 23.52655050901583 72-73 23.231586313813263 26.86928841847055 26.3922398155509 23.50688545216529 74-75 23.17236193223831 27.090005966961666 26.20751678579665 23.53011531500337 76-77 23.483447804648545 26.90271942611479 26.197058944026896 23.41677382520977 78-79 23.300838056945047 26.867850560059424 26.46240309078521 23.368908292210318 80-81 23.271722056017822 27.04664362934899 26.402048114026133 23.279586200607056 82-83 23.444202640429353 27.0377348639902 26.331598115999398 23.18646437958105 84-85 23.43626197671368 26.93436440400754 26.256022637384856 23.373350981893925 86-87 23.333390610811435 26.90691043961346 26.440319291098685 23.31937965847643 88-89 23.358676816396777 26.985918769554512 26.323428137875243 23.331976276173467 90-91 23.378374359330234 27.116217743454513 26.327950220248304 23.177457676966952 92-93 23.266352017734196 26.848815836521794 26.446637131702456 23.438195014041554 94-95 23.184028571768458 27.04212934752581 26.421549902121598 23.35229217858413 96-97 23.433693613432716 26.865563135375346 26.41846244949051 23.282280801701425 98-99 23.21948704729991 26.8913189315303 26.266469707634805 23.622724313534984 100-101 23.088050933761394 26.913502086002932 26.517382917179113 23.481064063056568 102-103 23.145642797566254 26.694680574042767 26.57485701142983 23.58481961696115 104-105 22.977861724668006 26.862204469422903 26.55978418188571 23.600149624023377 106-107 23.161172291907086 26.906375536846618 26.37471728391635 23.557734887329957 108-109 23.211415640993106 26.833259315653677 26.22796652286089 23.727358520492324 110-111 22.938894950438844 27.055917302688837 26.428166888164732 23.577020858707584 112-113 23.076653633751285 27.053080711549594 26.499380077351542 23.37088557734758 114-115 22.956569776931357 27.06797920572312 26.380673772104824 23.594777245240703 116-117 22.85722781698517 26.980876483493248 26.66831866360056 23.49357703592102 118-119 22.85367204529202 27.073163316426818 26.427900115397883 23.64526452288328 120-121 22.915232013915414 27.07392961136496 26.603782064075943 23.407056310643682 122-123 22.88492445309113 27.134570244351163 26.511995516880425 23.46850978567728 124-125 22.9717021966962 27.13842668680905 26.305108222361877 23.58476289413287 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 238.0 2 232.5 3 223.0 4 188.0 5 152.0 6 138.5 7 125.0 8 130.5 9 144.0 10 132.0 11 114.0 12 118.0 13 134.5 14 145.0 15 170.0 16 185.5 17 199.0 18 249.0 19 327.5 20 418.0 21 568.0 22 833.5 23 1141.5 24 1547.0 25 2140.5 26 2948.0 27 3873.5 28 4766.0 29 5958.0 30 7362.0 31 8694.5 32 10234.5 33 11756.0 34 13082.5 35 14373.5 36 16036.5 37 17547.5 38 18573.5 39 19311.0 40 20137.5 41 21816.0 42 23473.0 43 24826.0 44 26460.0 45 27635.0 46 28270.5 47 28983.0 48 29306.5 49 29264.0 50 28818.5 51 28263.0 52 28474.5 53 28978.0 54 28881.5 55 27201.5 56 24462.5 57 22127.5 58 20747.0 59 18766.0 60 15935.0 61 13248.0 62 10572.0 63 8309.5 64 6433.5 65 4960.0 66 3751.0 67 2901.0 68 2196.0 69 1586.0 70 1216.5 71 949.5 72 741.5 73 587.5 74 483.5 75 366.0 76 261.5 77 172.0 78 126.0 79 102.0 80 65.0 81 43.0 82 39.0 83 29.0 84 19.0 85 19.5 86 15.5 87 11.5 88 12.5 89 10.0 90 11.0 91 9.5 92 4.5 93 4.0 94 4.0 95 3.5 96 2.5 97 2.5 98 3.0 99 3.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05417934154921202 2 0.19279916907390326 3 0.02259674976808599 4 0.1070372357435652 5 0.02035028926482598 6 0.032507604929527215 7 0.03911484170382136 8 0.09976927529184165 9 0.11509806460820407 10-11 0.046052440316830215 12-13 0.030128999690781318 14-15 0.013280545916331238 16-17 0.029930782587552492 18-19 0.05041321658786435 20-21 0.19484741247393444 22-23 0.1070372357435652 24-25 0.07571893343341095 26-27 0.014734138006675952 28-29 0.006342947303322383 30-31 0.008721552542068276 32-33 0.006144730200093558 34-35 0.008655480174325336 36-37 0.0044268486387770796 38-39 0.009051914380782984 40-41 0.00535186178717826 42-43 0.010373361735641814 44-45 0.010373361735641814 46-47 0.01598951299379184 48-49 0.017443105084136552 50-51 0.011034085413071229 52-53 0.014271631432475363 54-55 0.013875197226017712 56-57 0.01651809193573537 58-59 0.017839539290594203 60-61 0.009250131484011808 62-63 0.019227059013195973 64-65 0.030459361529496024 66-67 0.026561091832662476 68-69 0.03382905228438604 70-71 0.024843210271346002 72-73 0.0151305722131336 74-75 0.010505506471127695 76-77 0.010439434103384755 78-79 0.023059256342286578 80-81 0.01949134848416774 82-83 0.021737808987427748 84-85 0.016055585361534782 86-87 0.02576822341974718 88-89 0.027353960245577777 90-91 0.02821290102623602 92-93 0.031912953619840735 94-95 0.02576822341974718 96-97 0.02708967077460601 98-99 0.032177243090812505 100-101 0.020548506368054803 102-103 0.028675407600436607 104-105 0.024182486593916586 106-107 0.03237546019404133 108-109 0.02246460503260011 110-111 0.020548506368054803 112-113 0.026428947097176594 114-115 0.012619822238901825 116-117 0.011364447251785937 118-119 0.03078972336821073 120-121 0.023125328710029522 122-123 0.017178815613164786 124-125 0.017509177451879493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 756746.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.33989068093015 #Duplication Level Percentage of deduplicated Percentage of total 1 84.4548914010287 59.40547828618187 2 9.591592334166215 13.493431124825985 3 2.5487313513147978 5.378324538796268 4 1.0901050885138186 3.0671149102715076 5 0.5890092029172421 2.0715421471630306 6 0.38088342731333297 1.6074779183638706 7 0.25029537845164257 1.2324024690761404 8 0.17888018559960153 1.0065930160048373 9 0.13575964305754662 0.8594386606394935 >10 0.7376916043274817 9.074475634839795 >50 0.030491524380515927 1.413415276739317 >100 0.011668858929123239 1.390306017097939 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.3214473548588297E-4 0.0 28-29 0.0 0.0 0.0 5.946513096864734E-4 0.0 30-31 0.0 0.0 0.0 0.0011893026193729467 0.0 32-33 0.0 0.0 0.0 0.002312532871002952 0.0 34-35 0.0 0.0 0.0 0.004625065742005904 0.0 36-37 0.0 0.0 0.0 0.010042999896927106 0.0 38-39 0.0 0.0 0.0 0.020218144529340093 0.0 40-41 0.0 0.0 0.0 0.03257367729727015 0.0 42-43 0.0 0.0 0.0 0.04466492059422845 0.0 44-45 0.0 0.0 0.0 0.05854011782024616 0.0 46-47 0.0 0.0 0.0 0.0753224992269533 0.0 48-49 0.0 0.0 0.0 0.09151022932397396 0.0 50-51 0.0 0.0 0.0 0.111794446221057 0.0 52-53 0.0 0.0 0.0 0.13168222891168238 0.0 54-55 0.0 0.0 0.0 0.15718616286045778 0.0 56-57 0.0 0.0 0.0 0.18876875464158382 0.0 58-59 0.0 0.0 0.0 0.2234567477066281 0.0 60-61 0.0 0.0 0.0 0.2591358262878165 0.0 62-63 0.0 0.0 0.0 0.2993738982432679 0.0 64-65 0.0 0.0 0.0 0.3378940886374028 0.0 66-67 0.0 0.0 0.0 0.38579655525103534 0.0 68-69 0.0 0.0 0.0 0.44347773229062326 0.0 70-71 0.0 0.0 0.0 0.5083607974141918 0.0 72-73 0.0 0.0 0.0 0.5792564480023681 0.0 74-75 0.0 0.0 0.0 0.650416388061516 0.0 76-77 0.0 0.0 0.0 0.7248138741400681 0.0 78-79 0.0 0.0 0.0 0.8163901758317851 0.0 80-81 0.0 0.0 0.0 0.924947076033438 0.0 82-83 0.0 0.0 0.0 1.0383933314480684 0.0 84-85 0.0 0.0 0.0 1.1602307775660525 0.0 86-87 0.0 0.0 0.0 1.2812753552711214 0.0 88-89 0.0 0.0 0.0 1.419366603853869 0.0 90-91 0.0 0.0 0.0 1.576420621978841 0.0 92-93 0.0 0.0 0.0 1.7463587518136865 0.0 94-95 0.0 0.0 0.0 1.9062538817516048 0.0 96-97 0.0 0.0 0.0 2.079165268134883 0.0 98-99 0.0 0.0 0.0 2.2658857793764353 0.0 100-101 0.0 0.0 0.0 2.498592658567075 0.0 102-103 0.0 0.0 0.0 2.7533677085838577 0.0 104-105 0.0 0.0 0.0 3.0000819297360013 0.0 106-107 0.0 0.0 0.0 3.2413121443654807 0.0 108-109 0.0 0.0 0.0 3.5040819508791587 0.0 110-111 0.0 0.0 0.0 3.801870112296596 0.0 112-113 0.0 0.0 0.0 4.137583812798482 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 855 0.0 53.01414 1 GTATCAA 1475 0.0 50.138798 1 ATCAACG 1470 0.0 47.85281 3 TCAACGC 1475 0.0 47.69376 4 CAACGCA 1520 0.0 46.254192 5 AACGCAG 1580 0.0 44.497707 6 TATCAAC 1705 0.0 41.634483 2 ACGCAGA 1950 0.0 35.44346 7 GTACATG 3345 0.0 33.69845 1 CGCAGAG 2050 0.0 33.423866 8 TACATGG 3445 0.0 32.2073 2 ACATGGG 3585 0.0 29.432482 3 GCAGAGT 2465 0.0 27.30065 9 CATGGGG 2445 0.0 27.065477 4 GTAGGAC 230 8.101779E-9 25.918804 3 GTGGTAT 525 0.0 24.992378 1 GGACCGA 175 1.7312435E-5 23.832668 6 CAGAGTA 2425 0.0 22.571302 10-11 GTGTAGC 565 0.0 22.167418 1 ATGGGGA 1390 0.0 21.432255 5 >>END_MODULE