FastQCFastQC Report
Fri 27 May 2016
SRR940077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756746
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.36696593044429704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21510.28424332603013425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12560.16597378777026903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.071.731111
GTATCAA25300.058.922171
TATCAAC32850.045.1864472
ATCAACG32400.045.0750853
TCAACGC32950.044.322694
CAACGCA33550.043.4667325
AACGCAG35000.041.9866646
ACGCAGA40450.036.3296247
CGCAGAG41500.035.267088
GGACCGA1501.4781835E-731.7308146
GCAGAGT46200.031.4217479
GTACATG33650.031.1879521
TACATGG34250.030.2853722
GTGGTAT6700.030.2596151
ACATGGG34700.029.3752863
TATAACG1254.8174574E-528.6144542
CATGGGG21550.028.4908524
ACCGAGT2205.153197E-927.0433068
CAGAGTA45150.026.22272310-11
GAGTACT28500.025.88566612-13
Produced by FastQC (version 0.11.5)