##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940077_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 756746 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.10225756066104 34.0 33.0 34.0 31.0 34.0 2 33.160639104798705 34.0 33.0 34.0 31.0 34.0 3 33.21573420936483 34.0 33.0 34.0 31.0 34.0 4 36.44954053275472 37.0 37.0 37.0 35.0 37.0 5 36.45033472261498 37.0 37.0 37.0 35.0 37.0 6 36.52613822867911 37.0 37.0 37.0 35.0 37.0 7 36.5179241119213 37.0 37.0 37.0 35.0 37.0 8 36.52194527622214 37.0 37.0 37.0 35.0 37.0 9 38.392295433342234 39.0 39.0 39.0 37.0 39.0 10-11 38.37149717342411 39.0 39.0 39.0 37.0 39.0 12-13 38.348215913926204 39.0 39.0 39.0 37.0 39.0 14-15 39.96731466040124 41.0 40.0 41.0 38.0 41.0 16-17 39.97599392662796 41.0 40.0 41.0 38.0 41.0 18-19 40.00555073961408 41.0 40.0 41.0 38.0 41.0 20-21 39.99588897727904 41.0 40.0 41.0 38.0 41.0 22-23 39.95503246796151 41.0 40.0 41.0 38.0 41.0 24-25 39.93061608518578 41.0 40.0 41.0 38.0 41.0 26-27 39.84322282509587 41.0 40.0 41.0 37.5 41.0 28-29 39.76392409077815 41.0 40.0 41.0 37.0 41.0 30-31 39.63597098630188 41.0 40.0 41.0 37.0 41.0 32-33 39.482172353735606 41.0 40.0 41.0 35.5 41.0 34-35 39.38598684367014 41.0 40.0 41.0 35.0 41.0 36-37 39.290429681821905 41.0 40.0 41.0 35.0 41.0 38-39 39.17670394029172 41.0 39.0 41.0 35.0 41.0 40-41 39.05435971911315 41.0 39.0 41.0 35.0 41.0 42-43 38.89620031027584 41.0 39.0 41.0 35.0 41.0 44-45 38.70440081612588 41.0 39.0 41.0 35.0 41.0 46-47 38.44399970399579 41.0 39.0 41.0 35.0 41.0 48-49 38.185116274152755 41.0 38.0 41.0 34.0 41.0 50-51 38.209160008774404 41.0 39.0 41.0 35.0 41.0 52-53 38.10280662203699 41.0 38.0 41.0 34.0 41.0 54-55 37.93331514140808 41.0 38.0 41.0 34.0 41.0 56-57 37.74376342920874 40.5 37.0 41.0 34.0 41.0 58-59 37.51840181514009 40.0 37.0 41.0 34.0 41.0 60-61 37.27654589518808 40.0 36.0 41.0 33.5 41.0 62-63 37.00947147391595 39.0 35.5 41.0 33.0 41.0 64-65 36.706605651037464 39.0 35.0 41.0 33.0 41.0 66-67 36.383343948960416 38.5 35.0 41.0 33.0 41.0 68-69 36.032263797892554 37.0 35.0 40.5 32.0 41.0 70-71 35.67257573875514 37.0 35.0 39.5 32.0 41.0 72-73 35.30349020675365 36.0 35.0 39.0 32.0 41.0 74-75 34.94477473286942 36.0 35.0 39.0 31.5 41.0 76-77 34.588708760931674 35.5 35.0 37.5 31.0 39.5 78-79 34.25988905128008 35.0 35.0 37.0 31.0 39.0 80-81 33.96154918559199 35.0 35.0 36.5 31.0 39.0 82-83 33.69797065329715 35.0 35.0 36.0 31.0 38.0 84-85 33.45970576653197 35.0 35.0 36.0 30.5 37.0 86-87 33.25950715299453 35.0 35.0 35.5 30.0 37.0 88-89 33.16514193666038 35.0 35.0 35.0 30.5 36.0 90-91 33.17426058413259 35.0 35.0 35.0 31.0 36.0 92-93 33.09188948471482 35.0 35.0 35.0 31.0 36.0 94-95 33.01971863742921 35.0 35.0 35.0 31.0 36.0 96-97 32.96021386303991 35.0 35.0 35.0 31.0 36.0 98-99 32.913622932925975 35.0 35.0 35.0 31.0 35.5 100-101 32.85659190798498 35.0 35.0 35.0 31.0 35.0 102-103 32.37332671728691 35.0 33.5 35.0 29.5 35.0 104-105 32.769926765387595 35.0 34.5 35.0 30.5 35.0 106-107 32.817718891147095 35.0 35.0 35.0 31.0 35.0 108-109 32.80781860756449 35.0 35.0 35.0 31.0 35.0 110-111 32.77339226107571 35.0 35.0 35.0 31.0 35.0 112-113 32.745089501629344 35.0 35.0 35.0 31.0 35.0 114-115 32.69258707677345 35.0 35.0 35.0 31.0 35.0 116-117 32.64166444223028 35.0 35.0 35.0 30.0 35.0 118-119 32.58797403620237 35.0 35.0 35.0 30.0 35.0 120-121 32.517682287055365 35.0 35.0 35.0 30.0 35.0 122-123 32.41455983910057 35.0 35.0 35.0 30.0 35.0 124-125 31.346326904932432 34.5 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2594395465652397 1101 2 0.21553744001834474 1101 3 0.19164073944530458 1101 4 0.04638327066126635 1101 5 0.13612655816736208 1101 6 0.17705134749127893 1101 7 0.19947820417982598 1101 8 0.15899670145456213 1101 9 0.2135380110523215 1101 10-11 0.2471546592268652 1101 12-13 0.18725475593554108 1101 14-15 0.3460653143797572 1101 16-17 0.3099690816662246 1101 18-19 0.2864738874283006 1101 20-21 0.26508189275381255 1101 22-23 0.3254065551450154 1101 24-25 0.29012560867682424 1101 26-27 0.32946824643541106 1101 28-29 0.3370668705721158 1101 30-31 0.36035412727514426 1101 32-33 0.3035626931725375 1101 34-35 0.27685817451973094 1101 36-37 0.21727538879425623 1101 38-39 0.3184823183245342 1101 40-41 0.1606011576196451 1101 42-43 0.20286080125104888 1101 44-45 0.13678821563143373 1101 46-47 0.13492394820026732 1101 48-49 0.09856082994111404 1101 50-51 0.10009724579325052 1101 52-53 0.07969816013151387 1101 54-55 0.06290385434230927 1101 56-57 0.11252157807211915 1101 58-59 0.058478059606599686 1101 60-61 -0.051435572020309905 1101 62-63 0.025234239899930344 1101 64-65 0.11278890441093381 1101 66-67 0.10059593248518439 1101 68-69 0.06012578004001057 1101 70-71 0.11887841462074533 1101 72-73 -0.021017309001202023 1101 74-75 -0.058219771890563266 1101 76-77 0.04908822528991408 1101 78-79 0.07485528313754486 1101 80-81 0.06764023655789231 1101 82-83 -0.019888875092732405 1101 84-85 -0.015633433194899737 1101 86-87 -0.057028093736910535 1101 88-89 -0.1295642227278364 1101 90-91 -0.02697993113679331 1101 92-93 -5.974611100540983E-4 1101 94-95 -0.07836982162390171 1101 96-97 -0.123013725915186 1101 98-99 -0.10531713268813547 1101 100-101 -0.05891033491623432 1101 102-103 -0.09920056961419732 1101 104-105 0.012861220778439986 1101 106-107 0.0034674989925704836 1101 108-109 -0.03204355585715035 1101 110-111 7.298447257610974E-4 1101 112-113 0.024581021050281038 1101 114-115 0.0279201818109982 1101 116-117 0.08233875555784209 1101 118-119 0.06217684646452426 1101 120-121 0.06499908959943923 1101 122-123 0.030197576656945557 1101 124-125 0.05835109211462708 1102 1 0.07974099141517144 1102 2 0.1478799215853428 1102 3 0.09049193479795292 1102 4 0.0668411167921974 1102 5 0.1348395059591283 1102 6 0.13023336879164304 1102 7 0.152627641879981 1102 8 0.13701208091385553 1102 9 0.14106044718846533 1102 10-11 0.18381912262151445 1102 12-13 0.17261075504182344 1102 14-15 0.16593502652692393 1102 16-17 0.2904480140412886 1102 18-19 0.2782442009756281 1102 20-21 0.12276836404299729 1102 22-23 0.18088665122008507 1102 24-25 0.181302202480353 1102 26-27 0.1555497892223343 1102 28-29 0.1740767201289799 1102 30-31 0.114584176802218 1102 32-33 0.05824076720247007 1102 34-35 0.2026048044445048 1102 36-37 0.19939281077981263 1102 38-39 0.15085482335172173 1102 40-41 0.14550447742303163 1102 42-43 0.09641483416979924 1102 44-45 0.005049228888303503 1102 46-47 0.1648054369915144 1102 48-49 0.13391170876735004 1102 50-51 0.19357726739151815 1102 52-53 0.012908761904064647 1102 54-55 0.12960713876920238 1102 56-57 0.05073608987669331 1102 58-59 0.08783355599447873 1102 60-61 0.0723972368722201 1102 62-63 -0.04232436403244577 1102 64-65 -0.0022009481250009344 1102 66-67 0.0019397732537811407 1102 68-69 0.09539170876571745 1102 70-71 0.04166924652660242 1102 72-73 0.15070408259781942 1102 74-75 0.22481185282084226 1102 76-77 0.045999765470142506 1102 78-79 -0.11289723976406663 1102 80-81 -0.0187016491946963 1102 82-83 -0.01818405458182326 1102 84-85 0.06254276960542882 1102 86-87 0.11380423105641313 1102 88-89 0.08579359175045198 1102 90-91 0.16443591938826785 1102 92-93 0.055163552146815675 1102 94-95 0.09032129515571796 1102 96-97 0.041598622338334224 1102 98-99 0.11270090000718369 1102 100-101 0.16288506800324853 1102 102-103 0.08006625283311308 1102 104-105 0.179200499843013 1102 106-107 0.1401068264670684 1102 108-109 0.1661147725110581 1102 110-111 0.050177785442222955 1102 112-113 0.03632159042542327 1102 114-115 -0.0388878356165705 1102 116-117 0.0327628078405553 1102 118-119 0.05155924759657893 1102 120-121 0.0814538077047473 1102 122-123 0.01137705643650122 1102 124-125 -0.14195542646733372 1103 1 0.0906833474903479 1103 2 0.10249180555751991 1103 3 0.0708271427304723 1103 4 -0.02126196149698245 1103 5 0.018548067450915084 1103 6 7.195312897962935E-4 1103 7 0.0219760953356527 1103 8 0.06328298516130104 1103 9 0.05605138251479502 1103 10-11 0.018263957607459247 1103 12-13 0.030313877729405192 1103 14-15 0.10734994808974818 1103 16-17 0.0992967079883087 1103 18-19 0.09289937338942167 1103 20-21 0.10070328338590429 1103 22-23 0.12117542582419816 1103 24-25 0.13565740819294803 1103 26-27 0.03795646008218512 1103 28-29 -0.06182216251124828 1103 30-31 0.08919152843756706 1103 32-33 0.08577702589715841 1103 34-35 0.0802687085439544 1103 36-37 -0.06629231205577213 1103 38-39 0.0012345221825640351 1103 40-41 -0.021297080220897158 1103 42-43 -0.117788954493804 1103 44-45 -0.3141359679446154 1103 46-47 -0.12023692648340045 1103 48-49 -0.10700049339336459 1103 50-51 -0.18450749655697507 1103 52-53 -0.05757832556079023 1103 54-55 -0.07690092694042505 1103 56-57 -0.18845567421980292 1103 58-59 -0.25297504989570996 1103 60-61 -0.21532984245292397 1103 62-63 -0.09717257699677617 1103 64-65 -0.022545590717868436 1103 66-67 -0.07124821697856731 1103 68-69 -0.16017427835874543 1103 70-71 -0.0913687141068138 1103 72-73 -0.1486220791603614 1103 74-75 -0.1762883950702374 1103 76-77 -0.08889275339917191 1103 78-79 -0.13405354063723252 1103 80-81 -0.004114924316787949 1103 82-83 -0.009968037858421042 1103 84-85 -0.17588820937933036 1103 86-87 -0.07623711495512708 1103 88-89 -0.0828301502948321 1103 90-91 -0.08878065231515109 1103 92-93 -0.07598516399940536 1103 94-95 -0.14742983330366144 1103 96-97 -0.19245495173299787 1103 98-99 -0.2850787174265861 1103 100-101 -0.23919101975758394 1103 102-103 -0.22411598410636202 1103 104-105 -0.18876050356086438 1103 106-107 -0.1747472137874908 1103 108-109 -0.2105062316327917 1103 110-111 -0.20617673579500462 1103 112-113 -0.2203913554085304 1103 114-115 -0.1619870290456049 1103 116-117 -0.17733875350323558 1103 118-119 -0.17179246174188734 1103 120-121 -0.2420717953735405 1103 122-123 -0.19018474983847966 1103 124-125 -0.23044005352075914 1104 1 0.07097662359907986 1104 2 0.11619888710258408 1104 3 0.11484538307382053 1104 4 0.05236554207955635 1104 5 -0.010023999787996729 1104 6 0.08892589752727531 1104 7 0.11290063038572384 1104 8 0.16021610390236418 1104 9 0.1922817115562836 1104 10-11 0.1302990076789854 1104 12-13 0.16223000791539022 1104 14-15 0.245820083998197 1104 16-17 0.22250620369646157 1104 18-19 0.22727437338942025 1104 20-21 0.1618129042728853 1104 22-23 0.2265044673120329 1104 24-25 0.182523466848167 1104 26-27 0.1943249329004928 1104 28-29 0.21484539828989568 1104 30-31 0.25070127450337054 1104 32-33 0.21481404306454266 1104 34-35 0.1722527929502533 1104 36-37 0.1838216900901557 1104 38-39 0.16640798427125958 1104 40-41 0.19219987972187624 1104 42-43 0.166619575549106 1104 44-45 0.2645858668193384 1104 46-47 0.17024724733633434 1104 48-49 0.24459777198574528 1104 50-51 0.12847310072484674 1104 52-53 0.01806321592705018 1104 54-55 0.04760282842437391 1104 56-57 0.07999255539392891 1104 58-59 0.1356783835806823 1104 60-61 0.25907108744693375 1104 62-63 0.2259586819445687 1104 64-65 0.06322649440373596 1104 66-67 0.11892971578252087 1104 68-69 0.022475042099053155 1104 70-71 0.1078869189403946 1104 72-73 0.03062505960931361 1104 74-75 -0.021650072466513848 1104 76-77 -0.11104046441491278 1104 78-79 -0.20689867654567706 1104 80-81 -0.14082808597630248 1104 82-83 0.002397841969909109 1104 84-85 0.12947883282382122 1104 86-87 0.056045610366759036 1104 88-89 0.09594086186539386 1104 90-91 0.07278721249171838 1104 92-93 0.07824185674451911 1104 94-95 0.0963916974545711 1104 96-97 0.08003253038430813 1104 98-99 0.07432087127155285 1104 100-101 0.11196911972739088 1104 102-103 0.021230045936555086 1104 104-105 0.12553670673957384 1104 106-107 0.0989071138233868 1104 108-109 -0.013141693006183175 1104 110-111 -0.04396732950029758 1104 112-113 -0.0861281222182626 1104 114-115 -0.02308323791541511 1104 116-117 -0.06048431859622383 1104 118-119 -0.018555694932160804 1104 120-121 -0.0744908762032992 1104 122-123 -0.015544638965799606 1104 124-125 0.03227230341772014 1105 1 0.14515862768905663 1105 2 0.18441207728663045 1105 3 0.2023581642599126 1105 4 0.1993555727543992 1105 5 0.06551920062099725 1105 6 0.12733336173995724 1105 7 0.09476412731824269 1105 8 0.1340018912234271 1105 9 0.20255778517591239 1105 10-11 0.18461904857878153 1105 12-13 0.1961193228438134 1105 14-15 0.2896403805217105 1105 16-17 0.27110985911568264 1105 18-19 0.2511304572340052 1105 20-21 0.2566556956839321 1105 22-23 0.28248606240406815 1105 24-25 0.24647413146984576 1105 26-27 0.26116095028290687 1105 28-29 0.3671012776354985 1105 30-31 0.3016247151986633 1105 32-33 0.24515018825881896 1105 34-35 0.3672617397805169 1105 36-37 0.3921518281269627 1105 38-39 0.48615491678660305 1105 40-41 0.3914266179631909 1105 42-43 0.44383391603990674 1105 44-45 0.3693053197845728 1105 46-47 0.44829875551629783 1105 48-49 0.349016867077772 1105 50-51 0.3766677581890576 1105 52-53 0.46109427420925186 1105 54-55 0.4304057425348091 1105 56-57 0.3387380819788035 1105 58-59 0.3420050706972475 1105 60-61 0.4085214964448909 1105 62-63 0.3912321993269856 1105 64-65 0.40422534409698585 1105 66-67 0.403381402899079 1105 68-69 0.3723919643894362 1105 70-71 0.47787349869499707 1105 72-73 0.34277996758476803 1105 74-75 0.3328712721142608 1105 76-77 0.3353680222926485 1105 78-79 0.44694846046864 1105 80-81 0.4359868424490472 1105 82-83 0.36828485628483065 1105 84-85 0.40485268251706685 1105 86-87 0.4453669293851661 1105 88-89 0.47846578517827965 1105 90-91 0.47095630758373375 1105 92-93 0.41903620746025894 1105 94-95 0.45708955021530784 1105 96-97 0.45241238723502875 1105 98-99 0.5248864387562122 1105 100-101 0.45589486103618526 1105 102-103 0.5361265183291906 1105 104-105 0.5531824122610445 1105 106-107 0.5794733203673488 1105 108-109 0.49726538777091633 1105 110-111 0.4721816991295569 1105 112-113 0.5381561313604735 1105 114-115 0.617445903188873 1105 116-117 0.5586491169866008 1105 118-119 0.6775332621230561 1105 120-121 0.5583727485410748 1105 122-123 0.5817297730996387 1105 124-125 0.537905611188684 1106 1 0.13793064658758425 1106 2 0.16626227452651676 1106 3 0.1409483242502887 1106 4 0.06620724255284927 1106 5 0.03893433354533471 1106 6 0.07217048846710128 1106 7 0.08759214492257428 1106 8 0.11229098557917183 1106 9 0.10524655266514316 1106 10-11 0.14546973114078554 1106 12-13 0.1287466576787466 1106 14-15 0.12970911374802085 1106 16-17 0.2248103365565015 1106 18-19 0.12002919083364105 1106 20-21 0.12714209629452 1106 22-23 0.0709094706927047 1106 24-25 0.20935113757163748 1106 26-27 0.08769355189981809 1106 28-29 0.11227398179227066 1106 30-31 0.018330373556779023 1106 32-33 -0.017697366671768577 1106 34-35 0.04337559551954939 1106 36-37 0.04957663430922565 1106 38-39 -0.025076971780123358 1106 40-41 0.07612694192606995 1106 42-43 0.14205377940306363 1106 44-45 0.03576043578485155 1106 46-47 0.07092866214364335 1106 48-49 0.04954304750975069 1106 50-51 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-0.3913641830857628 2216 42-43 -0.3911098618401425 2216 44-45 -0.3665044683844414 2216 46-47 -0.3459010471113828 2216 48-49 -0.31892082173349223 2216 50-51 -0.41900025771184346 2216 52-53 -0.38415024882979054 2216 54-55 -0.4191407480670648 2216 56-57 -0.41285575382048023 2216 58-59 -0.5011681905480785 2216 60-61 -0.6129472724664353 2216 62-63 -0.5539426273682864 2216 64-65 -0.5091890278447408 2216 66-67 -0.3151118032172846 2216 68-69 -0.3405116432720803 2216 70-71 -0.38189914869102637 2216 72-73 -0.32929692306444025 2216 74-75 -0.2435069217478656 2216 76-77 -0.2898933389965279 2216 78-79 -0.29792668402746614 2216 80-81 -0.2244690586087117 2216 82-83 -0.2102461332220571 2216 84-85 -0.2361163261641721 2216 86-87 -0.26188668437630014 2216 88-89 -0.2720557294911714 2216 90-91 -0.25225801048524943 2216 92-93 -0.24621146822102702 2216 94-95 -0.19272648525856795 2216 96-97 -0.18130705417958382 2216 98-99 -0.22787967263472808 2216 100-101 -0.29277190951064824 2216 102-103 -0.32666016375038964 2216 104-105 -0.3482413499645034 2216 106-107 -0.3340712037588176 2216 108-109 -0.30084381202566846 2216 110-111 -0.267918279490452 2216 112-113 -0.3480842159374973 2216 114-115 -0.3438587350605289 2216 116-117 -0.41189330658598067 2216 118-119 -0.4206501032802592 2216 120-121 -0.3444594354669306 2216 122-123 -0.38843638684924287 2216 124-125 -0.7034246226504024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 11.0 5 33.0 6 61.0 7 176.0 8 352.0 9 503.0 10 884.0 11 1299.0 12 1836.0 13 2945.0 14 5083.0 15 5324.0 16 2558.0 17 1936.0 18 1765.0 19 1554.0 20 1294.0 21 1260.0 22 1261.0 23 1425.0 24 1808.0 25 2230.0 26 2931.0 27 3683.0 28 4450.0 29 5498.0 30 6715.0 31 8520.0 32 10934.0 33 14521.0 34 21659.0 35 38475.0 36 98077.0 37 303767.0 38 200136.0 39 1775.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17245458330304 18.450049893934086 14.218779944621037 23.15871557814183 2 15.7489253116365 19.228233018073794 40.96885154698301 24.053990123306697 3 19.907472256212785 24.061970595153458 28.454329457968726 27.576227690665036 4 12.976149673601341 16.40224383362601 36.85108995846076 33.77051653431189 5 14.1680007720405 36.99726480856965 33.41071364832762 15.42402077106223 6 32.28528043157348 35.927144222140875 17.15377712697559 14.63379821931006 7 29.25525869974866 32.02263375029402 20.056135083634402 18.665972466322913 8 26.349528111149578 35.48826158314679 18.982062673605146 19.180147632098485 9 26.41560047889252 16.7752455909909 19.77598824440433 37.03316568571225 10-11 25.627291059351485 26.513651872623043 26.277905664516233 21.581151403509235 12-13 26.398553808014842 23.47168006173802 27.246328358524526 22.883437771722612 14-15 23.187701025178857 24.773702140480424 25.729240722778847 26.309356111561872 16-17 22.72479801677181 28.15858953995132 26.144109119836774 22.972503323440097 18-19 22.50669313085236 26.40773786713111 28.87197289447186 22.21359610754467 20-21 23.337024576277905 25.504991106659304 29.104085122352814 22.053899194709984 22-23 23.05568843442846 25.409648680006235 28.944916788460063 22.589746097105238 24-25 22.748451924423783 25.656032539319668 28.998105044493133 22.59741049176342 26-27 22.53728463711734 25.956067161240366 28.969693926363664 22.536954275278628 28-29 22.503683539369273 25.956762185412526 29.045319096921684 22.49423517829652 30-31 22.73181670720209 25.74042396023498 28.92425524829682 22.60350408426611 32-33 22.517264709691233 25.658146855087438 29.05393619523592 22.770652239985413 34-35 22.884666845062885 25.94627630931261 28.772072402435438 22.396984443189062 36-37 22.916376221439187 25.694210895280484 28.668546218948237 22.720866664332092 38-39 22.698035028415077 25.962085477155945 28.660675526295098 22.679203968133884 40-41 23.06865386051189 25.64699757051743 28.60023614279834 22.68411242617234 42-43 22.861881227370034 25.697002933636387 28.458387292861488 22.98272854613209 44-45 22.972421175040306 25.840315035547217 28.392909057271982 22.794354732140498 46-47 23.09434076955808 26.220488776947615 27.871042595534036 22.81412785796027 48-49 23.351514570868833 26.21937078161702 27.545599006253862 22.88351564126029 50-51 23.215447893093707 26.41523645914204 27.48681015543699 22.882505492327258 52-53 23.419879325427555 26.41176828156343 27.297864805364018 22.870487587645 54-55 23.149511196623436 26.311668644432874 27.40093769904301 23.13788245990068 56-57 23.135824868794973 26.577912626010015 27.48542918893061 22.800833316264402 58-59 23.3855227286843 26.20389543972693 27.39044653912685 23.02013529246192 60-61 23.218786938949187 26.329108404025963 27.60682851473792 22.845276142286934 62-63 23.05993230220728 26.3856210575418 27.709513964734512 22.844932675516404 64-65 22.920014377537214 26.13936696549391 27.72201930395805 23.218599353010823 66-67 23.01137377253248 26.336685867423597 27.514367531295385 23.137572828748542 68-69 22.933255102977974 26.2637274782141 27.738078955836187 23.064938462971735 70-71 22.892182417525785 26.2325789756408 27.574591684159543 23.300646922673877 72-73 23.00170730998248 26.368226591220832 27.431397060572504 23.19866903822419 74-75 22.977590895756304 26.50882858977781 27.217190444349885 23.296390070115997 76-77 23.135404291421256 26.425463084711765 27.219218758878792 23.219913864988193 78-79 23.085207601025452 26.42466368898169 27.285527393820864 23.204601316172003 80-81 23.04445613191216 26.603510292753445 27.147219806910112 23.20481376842428 82-83 23.123809045571434 26.62214270045696 27.185872142039734 23.068176111931876 84-85 23.218237090903322 26.450243708733538 27.092204954518934 23.2393142458442 86-87 23.12570491097061 26.31522264119528 27.28787589470422 23.27119655312989 88-89 23.12625372317792 26.53030210929427 27.167570096174938 23.175874071352872 90-91 23.18799595636575 26.63129587906098 27.122016002748616 23.058692161824656 92-93 23.10197485135261 26.34582132184018 27.189703735957043 23.362500090850165 94-95 22.964309382555026 26.419659605452072 27.26856467780524 23.347466334187654 96-97 23.079815023188175 26.400491051459312 27.171227767400712 23.3484661579518 98-99 22.97118187608524 26.27427168429037 27.010516078049967 23.744030361574424 100-101 22.92458022489757 26.281856029346763 27.15044509738756 23.64311864836811 102-103 23.01069315199552 26.009982213318604 27.249103397969726 23.73022123671615 104-105 22.8872699690517 26.1967687969279 27.230867424472677 23.685093809547723 106-107 22.837959179088845 26.24465721257307 27.110339090670628 23.807044517667457 108-109 22.952244024721736 26.13304649002369 26.977058261523396 23.937651223731173 110-111 22.80322591728268 26.378203518750016 27.12290517558071 23.69566538838659 112-113 22.77386885534021 26.289916874189373 27.204228111337446 23.73198615913297 114-115 22.699729032027875 26.308274467231772 27.10267776615616 23.889318734584194 116-117 22.549362926862788 26.216200501624066 27.296687642551888 23.93774892896126 118-119 22.528313680094563 26.331045245726603 27.075747394599638 24.064893679579196 120-121 22.51430632887807 26.369829751217548 27.245883853950897 23.86998006595348 122-123 22.462454327415443 26.382334868416706 27.151550390157848 24.003660414010003 124-125 22.42013268766861 26.41675239630018 27.06836368393615 24.09475123209506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 37.0 2 103.5 3 188.0 4 119.0 5 65.0 6 213.0 7 506.5 8 920.5 9 1055.5 10 780.5 11 522.5 12 440.5 13 510.5 14 585.5 15 616.5 16 666.5 17 742.0 18 837.5 19 919.0 20 1025.5 21 1181.5 22 1388.5 23 1701.5 24 2062.0 25 2613.0 26 3466.5 27 4371.5 28 5189.0 29 6279.0 30 7784.5 31 9117.0 32 10275.0 33 11591.5 34 13039.5 35 14314.5 36 15849.0 37 17366.0 38 18302.0 39 19038.5 40 19814.5 41 21399.0 42 23017.5 43 24322.0 44 26017.0 45 27298.5 46 27685.5 47 28111.0 48 28739.5 49 28901.5 50 28301.0 51 27844.5 52 28061.5 53 28635.0 54 28551.0 55 26847.5 56 24339.5 57 22196.5 58 20693.5 59 18380.0 60 15602.5 61 12971.5 62 10292.0 63 8093.0 64 6290.0 65 4662.0 66 3417.5 67 2705.0 68 1998.0 69 1405.0 70 1088.5 71 849.5 72 660.0 73 562.0 74 450.0 75 292.0 76 197.5 77 109.5 78 54.5 79 32.0 80 20.0 81 13.0 82 8.0 83 4.5 84 2.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0186324077035095 2 0.0019821710322882446 3 0.0 4 0.1420555906473242 5 0.040832723265137844 6 0.009646565690469457 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.3214473548588297E-4 30-31 6.607236774294149E-5 32-33 0.0 34-35 4.625065742005904E-4 36-37 7.267960451723563E-4 38-39 0.002312532871002952 40-41 6.607236774294149E-5 42-43 7.928684129152978E-4 44-45 7.928684129152978E-4 46-47 0.0 48-49 0.0017839539290594203 50-51 0.0011232302516300053 52-53 0.0 54-55 0.0 56-57 0.0013875197226017712 58-59 0.0019821710322882446 60-61 9.910855161441223E-4 62-63 6.607236774294149E-5 64-65 0.0013214473548588297 66-67 6.607236774294149E-4 68-69 8.589407806582393E-4 70-71 0.0012553749871158882 72-73 0.0 74-75 0.0 76-77 0.0035679078581188407 78-79 7.928684129152978E-4 80-81 0.0 82-83 0.0 84-85 3.3036183871470743E-4 86-87 1.9821710322882446E-4 88-89 0.0 90-91 1.3214473548588297E-4 92-93 7.267960451723563E-4 94-95 8.589407806582393E-4 96-97 3.3036183871470743E-4 98-99 0.0 100-101 1.9821710322882446E-4 102-103 0.0 104-105 0.0 106-107 1.9821710322882446E-4 108-109 3.964342064576489E-4 110-111 0.0 112-113 1.9821710322882446E-4 114-115 0.001916098664545303 116-117 5.285789419435319E-4 118-119 6.607236774294149E-5 120-121 6.607236774294149E-5 122-123 1.3214473548588297E-4 124-125 8.589407806582393E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 756746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.44409355223544 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13542005045387 56.069930473872006 2 10.333539882523874 13.938724611253924 3 2.7470788421681185 5.5582272727965965 4 1.196969757705936 3.229141612716629 5 0.6704363323120314 2.2608485358635124 6 0.4206845867719169 1.7023614375737204 7 0.27744428684738615 1.309838490636785 8 0.20307647691378133 1.095704712578516 9 0.15094909832603814 0.9162562598214211 >10 0.8134691977803409 9.752611016028414 >50 0.035260738375472625 1.578008664052968 >100 0.014887212332479211 1.6800738930392973 >500 1.958843721969529E-4 0.09128969551395688 >1k 5.876531165908587E-4 0.8169833242522567 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.36696593044429704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2151 0.28424332603013425 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1256 0.16597378777026903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.3214473548588297E-4 0.0 28-29 0.0 0.0 0.0 5.946513096864734E-4 0.0 30-31 0.0 0.0 0.0 0.0010571578838870638 0.0 32-33 0.0 0.0 0.0 0.002180388135517069 0.0 34-35 0.0 0.0 0.0 0.004492921006520021 0.0 36-37 0.0 0.0 0.0 0.009976927529184165 0.0 38-39 0.0 0.0 0.0 0.02035028926482598 0.0 40-41 0.0 0.0 0.0 0.032705822032756036 0.0 42-43 0.0 0.0 0.0 0.04479706532971433 0.0 44-45 0.0 0.0 0.0 0.058672262555732047 0.0 46-47 0.0 0.0 0.0 0.07512428212372448 0.0 48-49 0.0 0.0 0.0 0.09131201222074514 0.0 50-51 0.0 0.0 0.0 0.11153015675008524 0.0 52-53 0.0 0.0 0.0 0.1315500841761965 0.0 54-55 0.0 0.0 0.0 0.15692187338948604 0.0 56-57 0.0 0.0 0.0 0.1882401756996403 0.0 58-59 0.0 0.0 0.0 0.22286209639694166 0.0 60-61 0.0 0.0 0.0 0.2586733197136159 0.0 62-63 0.0 0.0 0.0 0.29904353640455317 0.0 64-65 0.0 0.0 0.0 0.3380262333728886 0.0 66-67 0.0 0.0 0.0 0.38566441051554945 0.0 68-69 0.0 0.0 0.0 0.44294915334867974 0.0 70-71 0.0 0.0 0.0 0.5074357842657906 0.0 72-73 0.0 0.0 0.0 0.5783975072217098 0.0 74-75 0.0 0.0 0.0 0.6494253025453718 0.0 76-77 0.0 0.0 0.0 0.7250120912432969 0.0 78-79 0.0 0.0 0.0 0.8167866100382427 0.0 80-81 0.0 0.0 0.0 0.924947076033438 0.0 82-83 0.0 0.0 0.0 1.038921910390012 0.0 84-85 0.0 0.0 0.0 1.1613540078176827 0.0 86-87 0.0 0.0 0.0 1.282662874993723 0.0 88-89 0.0 0.0 0.0 1.4211505577829286 0.0 90-91 0.0 0.0 0.0 1.579658167998245 0.0 92-93 0.0 0.0 0.0 1.7510498899234352 0.0 94-95 0.0 0.0 0.0 1.9124646843194415 0.0 96-97 0.0 0.0 0.0 2.0872260969995216 0.0 98-99 0.0 0.0 0.0 2.2763252134798204 0.0 100-101 0.0 0.0 0.0 2.5095606716124035 0.0 102-103 0.0 0.0 0.0 2.7652607347775873 0.0 104-105 0.0 0.0 0.0 3.013891054594276 0.0 106-107 0.0 0.0 0.0 3.257103440256044 0.0 108-109 0.0 0.0 0.0 3.5225161414794397 0.0 110-111 0.0 0.0 0.0 3.821361460780764 0.0 112-113 0.0 0.0 0.0 4.157735884960079 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1355 0.0 71.73111 1 GTATCAA 2530 0.0 58.92217 1 TATCAAC 3285 0.0 45.186447 2 ATCAACG 3240 0.0 45.075085 3 TCAACGC 3295 0.0 44.32269 4 CAACGCA 3355 0.0 43.466732 5 AACGCAG 3500 0.0 41.986664 6 ACGCAGA 4045 0.0 36.329624 7 CGCAGAG 4150 0.0 35.26708 8 GGACCGA 150 1.4781835E-7 31.730814 6 GCAGAGT 4620 0.0 31.421747 9 GTACATG 3365 0.0 31.187952 1 TACATGG 3425 0.0 30.285372 2 GTGGTAT 670 0.0 30.259615 1 ACATGGG 3470 0.0 29.375286 3 TATAACG 125 4.8174574E-5 28.614454 2 CATGGGG 2155 0.0 28.490852 4 ACCGAGT 220 5.153197E-9 27.043306 8 CAGAGTA 4515 0.0 26.222723 10-11 GAGTACT 2850 0.0 25.885666 12-13 >>END_MODULE