FastQCFastQC Report
Fri 27 May 2016
SRR940076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002884
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA250.002049875771.4604649
GGTATCA10000.053.7079931
GTATCAA16150.049.8835871
ATCAACG18200.043.9149673
TCAACGC18200.043.2595184
CAACGCA19150.041.688465
AACGCAG20350.039.230176
TATCAAC21000.037.2261732
CGCAGAG24800.031.9506578
ACGCAGA25100.031.8061357
GTACATG43100.031.5685021
TACATGG44300.030.0398372
ACATGGG45100.028.1696993
GGACGAT2151.21634E-724.9392787
GCAGAGT31500.023.8201569
CATGGGG25900.023.0292284
GTATTAG4400.021.7002011
GGACCGA1953.998154E-521.3866736
GAGTACA33300.020.9670851
GTATAGG4102.3646862E-1120.3770161
Produced by FastQC (version 0.11.5)