FastQCFastQC Report
Fri 27 May 2016
SRR940076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002884
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33330.3323415270360281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.24868279880823704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.1454804344271122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17250.075.73561
GTATCAA33100.058.7536241
ATCAACG42050.045.9508743
TCAACGC42200.045.7875444
CAACGCA43000.044.9844445
TATCAAC44100.044.08532
AACGCAG45050.043.0545126
CGCAGAG51850.037.0619248
ACGCAGA52100.036.9982767
GTACATG41300.035.3884121
TACATGG43250.032.95542
GCAGAGT58550.032.6176269
ACATGGG42750.032.5038343
GTGGTAT8250.028.9235171
TGGTATC7800.027.5247062
GAGTACT38700.027.3642312-13
CAGAGTA58000.027.02880310-11
AGAGTAC55150.026.48379310-11
CATGGGA19800.026.2040774
GTACTTT40200.026.12118514-15
Produced by FastQC (version 0.11.5)