FastQCFastQC Report
Fri 27 May 2016
SRR940075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436285
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.052.6515241
GTATCAA26050.049.4665451
TCAACGC27600.044.047034
ATCAACG27850.043.650123
CAACGCA28000.043.1808555
AACGCAG29500.040.783326
TATCAAC34650.036.48772
GTACATG68950.032.9652441
ACGCAGA37500.032.082887
CGCAGAG37400.032.009418
TACATGG67900.031.882382
ACATGGG70950.029.144563
GAGTACA50750.025.5087551
GCAGAGT46600.025.0465379
AGTACAT51900.024.3602872
GTATAGG5950.024.0635591
CATGGGG34650.023.9060124
GTGTAGC12550.023.7679561
CGCGATA700.003162974521.24284104-105
CATGGGA42800.020.8854144
Produced by FastQC (version 0.11.5)