FastQCFastQC Report
Fri 27 May 2016
SRR940075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436285
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61730.4297893523917607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.31546663788871987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.20044768273706123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.068.676051
GTATCAA56250.062.7293241
ATCAACG70300.049.8462943
TCAACGC71050.049.3201184
CAACGCA71600.048.885015
TATCAAC74000.047.6761172
AACGCAG74450.047.1522146
GTGGTAT10950.041.368611
ACGCAGA87900.039.8667647
CGCAGAG89000.039.440888
GCAGAGT101050.034.5021259
GTACATG72150.033.1268041
TACATGG74000.032.1330572
TGGTATC13150.031.7236542
ACATGGG72700.030.9861973
CATGGGG32850.027.938064
AGAGTAC96600.027.37658110-11
CAGAGTA97850.026.41882510-11
AGTACTT67400.025.9520712-13
GAGTACT65150.025.93513312-13
Produced by FastQC (version 0.11.5)