##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1436285 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.145696710611055 34.0 33.0 34.0 31.0 34.0 2 33.1921909648851 34.0 33.0 34.0 31.0 34.0 3 33.25104557939406 34.0 34.0 34.0 31.0 34.0 4 36.46960108892038 37.0 37.0 37.0 35.0 37.0 5 36.477302206734734 37.0 37.0 37.0 35.0 37.0 6 36.557797373084036 37.0 37.0 37.0 35.0 37.0 7 36.54870934389763 37.0 37.0 37.0 35.0 37.0 8 36.548926570979994 37.0 37.0 37.0 35.0 37.0 9 38.422641049652405 39.0 39.0 39.0 37.0 39.0 10-11 38.402738314471016 39.0 39.0 39.0 37.0 39.0 12-13 38.389545946661 39.0 39.0 39.0 37.0 39.0 14-15 40.0071817222905 41.0 40.0 41.0 38.0 41.0 16-17 40.017389654560205 41.0 40.0 41.0 38.0 41.0 18-19 40.060045186018094 41.0 40.0 41.0 38.0 41.0 20-21 40.05848456260422 41.0 40.0 41.0 38.0 41.0 22-23 40.018680484722736 41.0 40.0 41.0 38.0 41.0 24-25 39.99502988612984 41.0 40.0 41.0 38.0 41.0 26-27 39.916233895083494 41.0 40.0 41.0 38.0 41.0 28-29 39.83981486961153 41.0 40.0 41.0 37.5 41.0 30-31 39.70426969577765 41.0 40.0 41.0 37.0 41.0 32-33 39.546778320458685 41.0 40.0 41.0 35.5 41.0 34-35 39.447652450593026 41.0 40.0 41.0 35.0 41.0 36-37 39.34933596048138 41.0 40.0 41.0 35.0 41.0 38-39 39.23494118507121 41.0 40.0 41.0 35.0 41.0 40-41 39.11444316413525 41.0 39.0 41.0 35.0 41.0 42-43 38.96479876904654 41.0 39.0 41.0 35.0 41.0 44-45 38.769370981386004 41.0 39.0 41.0 35.0 41.0 46-47 38.50074497749402 41.0 39.0 41.0 35.0 41.0 48-49 38.242008375775 41.0 39.0 41.0 35.0 41.0 50-51 38.26086222441925 41.0 39.0 41.0 35.0 41.0 52-53 38.164209749457804 41.0 39.0 41.0 35.0 41.0 54-55 38.020455550256386 41.0 38.0 41.0 34.5 41.0 56-57 37.85142677114918 41.0 38.0 41.0 34.0 41.0 58-59 37.65593875867255 40.5 37.0 41.0 34.0 41.0 60-61 37.435165374560064 40.0 37.0 41.0 34.0 41.0 62-63 37.18175431756232 40.0 36.0 41.0 34.0 41.0 64-65 36.89295787395955 39.0 35.5 41.0 33.0 41.0 66-67 36.58397532523141 39.0 35.0 41.0 33.0 41.0 68-69 36.23741005441121 38.5 35.0 41.0 33.0 41.0 70-71 35.87526361411558 37.0 35.0 40.0 32.0 41.0 72-73 35.50640019216242 37.0 35.0 39.0 32.0 41.0 74-75 35.14192204193458 36.0 35.0 39.0 32.0 41.0 76-77 34.76817623243298 36.0 35.0 38.5 31.5 40.5 78-79 34.422955054184925 35.0 35.0 37.0 31.0 39.0 80-81 34.10301367764754 35.0 35.0 37.0 31.0 39.0 82-83 33.812939284334234 35.0 35.0 36.5 31.0 39.0 84-85 33.55303473892717 35.0 35.0 36.0 31.0 37.0 86-87 33.33944586206776 35.0 35.0 36.0 31.0 37.0 88-89 33.22654278224726 35.0 35.0 35.5 31.0 37.0 90-91 33.20964049614109 35.0 35.0 35.0 31.0 36.0 92-93 33.11689636806066 35.0 35.0 35.0 31.0 36.0 94-95 33.03407088426044 35.0 35.0 35.0 31.0 36.0 96-97 32.96886899187835 35.0 35.0 35.0 31.0 36.0 98-99 32.9171376154454 35.0 35.0 35.0 31.0 36.0 100-101 32.863160514800335 35.0 35.0 35.0 31.0 35.5 102-103 32.39649790953746 35.0 33.5 35.0 29.5 35.0 104-105 32.769603177642324 35.0 35.0 35.0 31.0 35.0 106-107 32.81465099196886 35.0 35.0 35.0 31.0 35.0 108-109 32.80779058473771 35.0 35.0 35.0 31.0 35.0 110-111 32.77193071013065 35.0 35.0 35.0 31.0 35.0 112-113 32.744842771455524 35.0 35.0 35.0 31.0 35.0 114-115 32.696025510257364 35.0 35.0 35.0 31.0 35.0 116-117 32.645564424887816 35.0 35.0 35.0 31.0 35.0 118-119 32.59254535137525 35.0 35.0 35.0 31.0 35.0 120-121 32.52302154516687 35.0 35.0 35.0 30.5 35.0 122-123 32.42974827419349 35.0 35.0 35.0 30.0 35.0 124-125 31.404345237888023 34.5 33.0 35.0 26.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13369864713315138 1101 2 0.05448982962440141 1101 3 0.11397501328537629 1101 4 -0.058858706940640104 1101 5 -0.004476882573953844 1101 6 0.07991447997394374 1101 7 0.009327334215548433 1101 8 0.09101954242571253 1101 9 0.12704557773183467 1101 10-11 0.15148565919292167 1101 12-13 0.13317513079153542 1101 14-15 0.15490203540590386 1101 16-17 0.22241272083530106 1101 18-19 0.16213967641123617 1101 20-21 0.1880404743163595 1101 22-23 0.19477542505940448 1101 24-25 0.20094614947159073 1101 26-27 0.21777729028335102 1101 28-29 0.16454613331986678 1101 30-31 0.1960422714953296 1101 32-33 0.22443783511203463 1101 34-35 0.19956431375869244 1101 36-37 0.1611161136082231 1101 38-39 0.2668999798650873 1101 40-41 0.13100373501271179 1101 42-43 0.2174565851706305 1101 44-45 0.28428625602683866 1101 46-47 0.4238689987582589 1101 48-49 0.4819907691557077 1101 50-51 0.4257329300823045 1101 52-53 0.46123546176274743 1101 54-55 0.45051226215453966 1101 56-57 0.5260283601485654 1101 58-59 0.4689071457767682 1101 60-61 0.4368349910377063 1101 62-63 0.3833195734840942 1101 64-65 0.4857699735709389 1101 66-67 0.5156487486031622 1101 68-69 0.46937037490884137 1101 70-71 0.514999388043833 1101 72-73 0.4876514652147108 1101 74-75 0.4495117394425705 1101 76-77 0.37294323667420315 1101 78-79 0.339326419870865 1101 80-81 0.3370889446163403 1101 82-83 0.38496792692366455 1101 84-85 0.3310865524917901 1101 86-87 0.2548411083574038 1101 88-89 0.2943927663173085 1101 90-91 0.25830207969295316 1101 92-93 0.2745736847689244 1101 94-95 0.34075868407106924 1101 96-97 0.2909925959258288 1101 98-99 0.32030810704660695 1101 100-101 0.3452920267443247 1101 102-103 0.39762538938037295 1101 104-105 0.39139683567444905 1101 106-107 0.4177089943955963 1101 108-109 0.3757861368153357 1101 110-111 0.42272860863987916 1101 112-113 0.4205843605585784 1101 114-115 0.40884407917333476 1101 116-117 0.33124767902859276 1101 118-119 0.35881628412047206 1101 120-121 0.36674418831150746 1101 122-123 0.4022458441035113 1101 124-125 0.2635419551726663 1102 1 0.08247086088311306 1102 2 0.04894556218351909 1102 3 0.07984686823630938 1102 4 -0.0470254528958165 1102 5 0.0020566386805569437 1102 6 0.009299542019057583 1102 7 0.08862992139215464 1102 8 0.10420218313316809 1102 9 0.05900714577693833 1102 10-11 0.1031222629178501 1102 12-13 0.10687765216608369 1102 14-15 0.11622776889870323 1102 16-17 0.10975671884344962 1102 18-19 0.10632019633636247 1102 20-21 0.11161116928384018 1102 22-23 0.12860323709674049 1102 24-25 0.08927273216654896 1102 26-27 0.1274228484225688 1102 28-29 0.07948808968598797 1102 30-31 -0.0103927689862644 1102 32-33 0.047764884645864925 1102 34-35 0.02705400995773033 1102 36-37 3.868223585570263E-4 1102 38-39 0.04602516826791714 1102 40-41 -0.0036848389945873805 1102 42-43 -0.0297390469958998 1102 44-45 -0.06356761895816021 1102 46-47 -0.04549970169568951 1102 48-49 -0.0054834991299301805 1102 50-51 -0.12485906607627584 1102 52-53 -0.08604975900757239 1102 54-55 -0.16535714744988894 1102 56-57 -0.1558654051070576 1102 58-59 -0.22720075180323107 1102 60-61 -0.21131410396179007 1102 62-63 -0.2056954854042914 1102 64-65 -0.1505580941162563 1102 66-67 -0.14852848121939388 1102 68-69 -0.18484095638226705 1102 70-71 -0.2602966100354678 1102 72-73 -0.23012553696831617 1102 74-75 -0.14715725400834856 1102 76-77 -0.23147639367832795 1102 78-79 -0.2453492604912313 1102 80-81 -0.15321859105236513 1102 82-83 -0.2598877309412444 1102 84-85 -0.2563056855337962 1102 86-87 -0.1908055079499391 1102 88-89 -0.08772930538025037 1102 90-91 -0.09067554316348492 1102 92-93 -0.12611857059638965 1102 94-95 -0.13690863320596947 1102 96-97 -0.19775102950043788 1102 98-99 -0.22011124764868129 1102 100-101 -0.1306035258372944 1102 102-103 -0.11826602834261024 1102 104-105 -0.15687652738836988 1102 106-107 -0.14263548823577565 1102 108-109 -0.14641649793436784 1102 110-111 -0.13466367384655342 1102 112-113 -0.2070371620475413 1102 114-115 -0.17076417631646734 1102 116-117 -0.16713616923542673 1102 118-119 -0.2646025770594278 1102 120-121 -0.2038229542344041 1102 122-123 -0.2100622517718591 1102 124-125 -0.15749336007695902 1103 1 0.12398856927647017 1103 2 0.10536139439157921 1103 3 0.07245448771817564 1103 4 0.05941906447937839 1103 5 0.12910687655449493 1103 6 0.10371069441119829 1103 7 0.07988094541504154 1103 8 0.12760627321210904 1103 9 0.15812191400329567 1103 10-11 0.14772707150385855 1103 12-13 0.16635020835268932 1103 14-15 0.20500773487734847 1103 16-17 0.22801570456600473 1103 18-19 0.2207480211990145 1103 20-21 0.16507017454738104 1103 22-23 0.18396936755537752 1103 24-25 0.15553703403972463 1103 26-27 0.19433194024266243 1103 28-29 0.22560801372666361 1103 30-31 0.17078475086161404 1103 32-33 0.1798456741017489 1103 34-35 0.14924749829952333 1103 36-37 0.14720289406897535 1103 38-39 0.1854582541655887 1103 40-41 0.14656155281222283 1103 42-43 0.19495850612176469 1103 44-45 0.11684235345898486 1103 46-47 0.24939884801232637 1103 48-49 0.3555008530487669 1103 50-51 0.30619632260147966 1103 52-53 0.2519745595004892 1103 54-55 0.2556033171245602 1103 56-57 0.21735210612273903 1103 58-59 0.2101578578805814 1103 60-61 0.17325502979627316 1103 62-63 0.20862824612257924 1103 64-65 0.23698039286017547 1103 66-67 0.2162391188216901 1103 68-69 0.2118382657862341 1103 70-71 0.1853058536320802 1103 72-73 0.25449940133978544 1103 74-75 0.24398065776158973 1103 76-77 0.2918541045416916 1103 78-79 0.2932053578206819 1103 80-81 0.28326537155921017 1103 82-83 0.3715783316249883 1103 84-85 0.3275799520911207 1103 86-87 0.3110380464053222 1103 88-89 0.2654533702819464 1103 90-91 0.24302564507885904 1103 92-93 0.20582799983509403 1103 94-95 0.18069566681331395 1103 96-97 0.14812319167847932 1103 98-99 0.24421517702707973 1103 100-101 0.19920888068539 1103 102-103 0.20995648087045993 1103 104-105 0.23507715796666417 1103 106-107 0.2796790665428759 1103 108-109 0.2857123924131315 1103 110-111 0.23090654719207748 1103 112-113 0.23264475828034392 1103 114-115 0.16428577846368597 1103 116-117 0.16535028359939474 1103 118-119 0.2383924550203531 1103 120-121 0.28205523777332786 1103 122-123 0.2927887281585555 1103 124-125 0.27845447801343326 1104 1 0.06858279425986069 1104 2 0.075287253024797 1104 3 0.10010975478338935 1104 4 -0.012485362512379083 1104 5 -0.010297696961700353 1104 6 0.045662595321601884 1104 7 0.06947771015539672 1104 8 0.10388396633132402 1104 9 0.13851694389428104 1104 10-11 0.09270775469848047 1104 12-13 0.13430165015318352 1104 14-15 0.19379373104817432 1104 16-17 0.2012249316984267 1104 18-19 0.24671886771954377 1104 20-21 0.1900580862810628 1104 22-23 0.2021634294082233 1104 24-25 0.15885398105648818 1104 26-27 0.20121553634316314 1104 28-29 0.2622623234988666 1104 30-31 0.2511253225022969 1104 32-33 0.2531738302381896 1104 34-35 0.2454731508217023 1104 36-37 0.2866123517331758 1104 38-39 0.24626894947289912 1104 40-41 0.2179207867824502 1104 42-43 0.279155112615733 1104 44-45 0.26199129555252654 1104 46-47 0.20703863232834152 1104 48-49 0.21850782761212173 1104 50-51 0.10199497848394401 1104 52-53 0.1034366292070601 1104 54-55 0.13712426523537147 1104 56-57 0.1809017095299268 1104 58-59 0.14520479454294843 1104 60-61 0.07388215440065693 1104 62-63 0.14520514214884628 1104 64-65 0.11905324969602304 1104 66-67 0.056291208260205394 1104 68-69 0.09037988361142624 1104 70-71 0.1567018408160763 1104 72-73 0.050112459597897896 1104 74-75 0.046198305165169984 1104 76-77 0.039736826379687784 1104 78-79 -0.04474532693686939 1104 80-81 0.07764029829698416 1104 82-83 0.05121729540415032 1104 84-85 0.025257906023846033 1104 86-87 0.07747869912285665 1104 88-89 0.08955322180385394 1104 90-91 0.022904420525271973 1104 92-93 0.0661358721048515 1104 94-95 0.02680959444978015 1104 96-97 -0.013852861343828238 1104 98-99 0.07489947058390811 1104 100-101 0.031731445449189266 1104 102-103 -0.010583559374914842 1104 104-105 0.04431882072601212 1104 106-107 0.038009591491103834 1104 108-109 -0.06318183647757536 1104 110-111 -0.04804240857707498 1104 112-113 -0.0288036846532691 1104 114-115 -0.06643038415890601 1104 116-117 -0.01905805071076827 1104 118-119 0.008467524375255664 1104 120-121 0.043018929017030416 1104 122-123 -0.09491433029492669 1104 124-125 -0.02709415833244222 1105 1 0.05859240733364146 1105 2 0.06088386441629012 1105 3 0.07908566201722778 1105 4 -0.07356443004422175 1105 5 -0.037408667882154134 1105 6 0.048612607337943814 1105 7 0.04447410525273199 1105 8 0.06351987116189406 1105 9 0.0735421782129535 1105 10-11 0.06777008947377539 1105 12-13 0.09208914114092437 1105 14-15 0.13038589639304377 1105 16-17 0.16164340081642337 1105 18-19 0.12982343993775913 1105 20-21 0.17675824850167743 1105 22-23 0.1639477060244232 1105 24-25 0.1653306393117191 1105 26-27 0.2244222474702866 1105 28-29 0.26849189571708365 1105 30-31 0.21770637273060345 1105 32-33 0.2727178100507359 1105 34-35 0.21932483911778178 1105 36-37 0.24843973697711164 1105 38-39 0.28982503575024054 1105 40-41 0.2884033931270835 1105 42-43 0.35297540821774476 1105 44-45 0.30634917226485214 1105 46-47 0.29979638286908283 1105 48-49 0.2815305384989344 1105 50-51 0.22397647331691672 1105 52-53 0.25724731173529136 1105 54-55 0.2920892676386373 1105 56-57 0.26168517504302 1105 58-59 0.2866596731778941 1105 60-61 0.3522094795628803 1105 62-63 0.32637132816181946 1105 64-65 0.29930183777581476 1105 66-67 0.32469108208861996 1105 68-69 0.3729211897878244 1105 70-71 0.3632405334380451 1105 72-73 0.30184281287836257 1105 74-75 0.3277204753165819 1105 76-77 0.23462327194565802 1105 78-79 0.2457153495832003 1105 80-81 0.14191030550679784 1105 82-83 0.13073063354401881 1105 84-85 0.1282134755984643 1105 86-87 0.14764557607551154 1105 88-89 0.12004408938375377 1105 90-91 0.14495260605759341 1105 92-93 0.14492477501281087 1105 94-95 0.13690001742502744 1105 96-97 0.14835048520748018 1105 98-99 0.1491344801969845 1105 100-101 0.16163771797983628 1105 102-103 0.22480607052174406 1105 104-105 0.23180214805118027 1105 106-107 0.2090362377299826 1105 108-109 0.23447738005691576 1105 110-111 0.2345604813532276 1105 112-113 0.23407182604127996 1105 114-115 0.22717301646594024 1105 116-117 0.2827678324903786 1105 118-119 0.25113004540386186 1105 120-121 0.2884434063061363 1105 122-123 0.37966855242557074 1105 124-125 0.4332137604370878 1106 1 0.045391829627831726 1106 2 0.040434245906361355 1106 3 0.07991574940252377 1106 4 0.011826615515758476 1106 5 0.012712854576889754 1106 6 -0.03808411170154358 1106 7 0.01670600453351767 1106 8 0.004373008425375247 1106 9 -0.058108805783426476 1106 10-11 0.08173496029233718 1106 12-13 0.08049826189019171 1106 14-15 0.08711529374085103 1106 16-17 0.09858389420052305 1106 18-19 0.08029479535813522 1106 20-21 0.07174125286365296 1106 22-23 0.1645385415095646 1106 24-25 0.10734954604723868 1106 26-27 0.10857516444696813 1106 28-29 0.057749193629469175 1106 30-31 0.07543975274723635 1106 32-33 0.09559087524687726 1106 34-35 0.053025803419650686 1106 36-37 0.07286837718017836 1106 38-39 0.06732981349396994 1106 40-41 0.08989142323780897 1106 42-43 0.08905401122006396 1106 44-45 -0.011987005831926467 1106 46-47 0.04690887502774643 1106 48-49 0.10050365183722221 1106 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2216 96-97 -0.22201520718367362 2216 98-99 -0.1911027875366358 2216 100-101 -0.25596722573371977 2216 102-103 -0.2730547900506153 2216 104-105 -0.2474021276846159 2216 106-107 -0.18474097303903392 2216 108-109 -0.20191240361323537 2216 110-111 -0.27858511745323256 2216 112-113 -0.3252417166143573 2216 114-115 -0.2553851348971392 2216 116-117 -0.35359101058570275 2216 118-119 -0.2872892716990876 2216 120-121 -0.32881373122268087 2216 122-123 -0.3145382663727503 2216 124-125 -0.4823144336494458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 12.0 5 52.0 6 133.0 7 265.0 8 569.0 9 1010.0 10 1890.0 11 2904.0 12 3862.0 13 6006.0 14 10697.0 15 11212.0 16 5155.0 17 3803.0 18 3397.0 19 2922.0 20 2501.0 21 2400.0 22 2405.0 23 2624.0 24 2908.0 25 3851.0 26 4861.0 27 6198.0 28 7502.0 29 9429.0 30 11622.0 31 14540.0 32 18882.0 33 25348.0 34 36958.0 35 66121.0 36 161740.0 37 561196.0 38 436799.0 39 4491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8523166056774 18.985129296087518 14.054086048835519 23.108468049399566 2 15.166123932721879 19.924288788766834 41.637824134706634 23.27176314380465 3 19.387166847689866 25.022976021388587 29.0537360403264 26.53612109059515 4 12.26121655532678 16.766525100805538 38.048259757899366 32.92399858596832 5 13.335984737939302 37.93108251835145 34.03428391528414 14.698648828425101 6 31.24867693918934 37.04027118568532 17.596082068765735 14.114969806359607 7 28.056479041415876 32.95244328249616 20.846907124978678 18.144170551109283 8 25.800728963959102 35.87978708960966 19.74984073495163 18.569643211479615 9 26.583164204875775 16.587654957059357 20.356753708351754 36.47242712971311 10-11 24.891543112961564 27.115474992776505 27.151958002764076 20.841023891497855 12-13 25.78464580497603 24.19182126110069 28.05738415425908 21.966148779664202 14-15 22.680874617502795 25.40028615490658 26.583338265037927 25.335500962552697 16-17 21.771062149921498 28.724278259537627 27.219597781777296 22.285061808763583 18-19 21.611623041388025 26.892782421316102 30.135140309896713 21.36045422739916 20-21 22.49598791326234 26.10554311992397 30.265650619480116 21.132818347333572 22-23 22.026965400320968 26.144532596246567 30.074428125337242 21.754073878095227 24-25 21.784325534277666 26.328792683903263 30.204799186790922 21.682082595028145 26-27 21.586662814135078 26.583895257556822 30.11588925596243 21.71355267234567 28-29 21.516409921321078 26.634212467550732 30.16600865917208 21.683368951956112 30-31 21.857356802245796 26.323050540875304 30.066532849027666 21.753059807851237 32-33 21.58484783291315 26.442107857574033 30.0523991441798 21.920645165333017 34-35 21.993989318908998 26.5710676182476 29.87011901956204 21.564824043281362 36-37 22.032811448010712 26.340889263625773 29.734791650856003 21.89150763750751 38-39 21.81495368097839 26.549102340494418 29.84205231038096 21.79389166814623 40-41 22.140684621107184 26.37243970288596 29.74579879250858 21.741076883498277 42-43 21.96819528429642 26.39432834469962 29.475096865829077 22.162379505174883 44-45 22.029918479783912 26.66954217157713 29.315250376061037 21.98528897257792 46-47 22.24408420903123 26.798618378133 28.850303178412613 22.106994234423162 48-49 22.30516945290685 26.95041164361887 28.632529959000998 22.111888944473282 50-51 22.415599163519886 27.04865043256549 28.347165008478637 22.188585395435982 52-53 22.489068147710288 27.106676984956675 28.30205297070323 22.10220189662981 54-55 22.284261132017672 27.090271081296542 28.37083169426681 22.254636092418984 56-57 22.3422717134334 27.197731329192255 28.256488468831716 22.203508488542635 58-59 22.36479227545565 26.96168432720419 28.348870289565976 22.324653107774186 60-61 22.377207548746227 27.084312435379264 28.482000187987595 22.056479827886914 62-63 22.24932151207075 27.093356318605956 28.593758050299318 22.06356411902398 64-65 22.11325502362729 26.89047897216596 28.551177604658758 22.445088399547995 66-67 22.184292261986403 27.19911186800838 28.223673354578132 22.39292251542708 68-69 22.29390873158162 26.99804145323752 28.479483996382292 22.228565818798568 70-71 22.199853160240192 26.865305329573104 28.495477314510364 22.43936419567634 72-73 22.235071730192825 27.065450102173315 28.285054846357095 22.414423321276765 74-75 22.25000226278526 27.223221328944224 28.047049223586157 22.479727184684357 76-77 22.29880863813075 27.112403552814396 28.12165461472128 22.46713319433357 78-79 22.305013893636914 27.079328454488678 28.150854749354053 22.46480290252035 80-81 22.205446690594137 27.317802525264835 28.009970166088205 22.466780618052823 82-83 22.474334829090324 27.206334397421124 27.97940520161389 22.339925571874662 84-85 22.401029047260977 27.23452251650096 27.818635642075364 22.545812794162696 86-87 22.24768151056897 27.224705489180383 28.05514941376333 22.472463586487315 88-89 22.347862715268906 27.227848233463416 27.973626404230355 22.450662647037323 90-91 22.3178239656892 27.273986837547625 28.04928696130462 22.35890223545855 92-93 22.314765110484025 27.079081872432155 28.098248868075732 22.50790414900809 94-95 22.156538921330196 27.036613614859057 28.0931357566027 22.713711707208052 96-97 22.311863611409628 26.975826763402782 28.122958047481738 22.589351577705855 98-99 22.058888034060093 26.999690172911368 27.96955339643594 22.971868396592598 100-101 22.070959652045804 26.975501312069156 28.120750744457386 22.832788291427654 102-103 22.257421055013456 26.780165496402176 28.077714381198714 22.88469906738565 104-105 22.11315999261985 26.77247203723495 28.08774024653881 23.02662772360639 106-107 22.078827193285992 26.876530428237928 27.987281040409222 23.057361338066855 108-109 22.300410400907623 26.762323602281867 27.74569834471518 23.19156765209533 110-111 22.029297806493837 26.9773060360583 27.840714064409223 23.15268209303864 112-113 22.067362236946224 26.967313813223683 28.032217963147144 22.933105986682953 114-115 21.974177269480187 27.026278081732062 27.842568236537545 23.156976412250202 116-117 21.85856096249145 26.985025473926434 27.987864419878537 23.16854914370358 118-119 21.88452874249058 27.019009879344512 27.798175772891 23.298285605273904 120-121 21.799394687537205 27.092536777222875 27.885416731939316 23.222651803300604 122-123 21.77271206614033 27.105030740882956 27.862473843008967 23.259783349967748 124-125 21.836388701288755 27.03718660140503 27.785687927757312 23.3407367695489 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 136.0 1 105.0 2 205.5 3 325.0 4 201.5 5 158.0 6 424.5 7 1107.5 8 2011.0 9 2251.0 10 1652.5 11 1111.0 12 1018.5 13 1107.0 14 1265.5 15 1406.0 16 1533.5 17 1663.5 18 1832.5 19 2053.5 20 2321.5 21 2658.0 22 3150.5 23 3962.0 24 5010.5 25 6199.5 26 7918.5 27 10091.0 28 12080.0 29 14617.0 30 17713.5 31 20725.5 32 24044.5 33 27064.5 34 29996.5 35 33176.5 36 37045.5 37 40537.0 38 42352.0 39 43318.0 40 44388.5 41 46532.0 42 48370.0 43 49791.5 44 51886.5 45 54438.5 46 54856.5 47 54087.5 48 53905.5 49 53077.0 50 51943.5 51 50344.0 52 48763.0 53 47937.0 54 46950.0 55 43692.0 56 38797.0 57 34260.5 58 31204.0 59 27363.5 60 22642.0 61 18529.0 62 14705.0 63 11226.0 64 8153.5 65 5961.0 66 4234.0 67 3265.5 68 2487.0 69 1648.0 70 1232.0 71 996.5 72 792.5 73 728.5 74 580.0 75 407.0 76 273.0 77 155.0 78 93.0 79 41.5 80 23.5 81 10.0 82 7.5 83 8.5 84 4.0 85 1.5 86 1.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01670977556682692 2 0.0026457144647475954 3 3.4812032430889413E-4 4 0.14537504743139418 5 0.03954646884149037 6 0.015386918334453121 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.133082918780047E-4 30-31 1.3924812972355765E-4 32-33 1.3924812972355765E-4 34-35 5.9180455132512E-4 36-37 0.0012532331675120189 38-39 0.0034812032430889413 40-41 1.0443609729266824E-4 42-43 0.0013924812972355765 44-45 0.0010791730053575717 46-47 1.3924812972355765E-4 48-49 0.002123533978284254 50-51 0.0019842858485606967 52-53 3.481203243088941E-5 54-55 0.0 56-57 0.00177541365397536 58-59 0.0022279700755769224 60-61 0.0013924812972355765 62-63 2.0887219458533648E-4 64-65 0.0015317294269591342 66-67 8.354887783413459E-4 68-69 0.0011139850377884612 70-71 0.00177541365397536 72-73 0.0 74-75 1.3924812972355765E-4 76-77 0.0049781206376171856 78-79 0.0011139850377884612 80-81 0.0 82-83 0.0 84-85 3.4812032430889413E-4 86-87 3.4812032430889413E-4 88-89 0.0 90-91 1.7406016215444707E-4 92-93 9.747369080649036E-4 94-95 0.0011139850377884612 96-97 4.5255642160156237E-4 98-99 0.0 100-101 2.784962594471153E-4 102-103 0.0 104-105 0.0 106-107 2.784962594471153E-4 108-109 4.5255642160156237E-4 110-111 0.0 112-113 3.8293235673978355E-4 114-115 0.0021583460107151434 116-117 8.703008107722353E-4 118-119 3.8293235673978355E-4 120-121 1.3924812972355765E-4 122-123 3.8293235673978355E-4 124-125 0.0010443609729266824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1436285.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.4402566420487 #Duplication Level Percentage of deduplicated Percentage of total 1 71.63419176970095 38.28149592518552 2 15.403340351163223 16.463169230219744 3 5.558110074705998 8.910804865111349 4 2.561642990647525 5.475794353820355 5 1.3972875040146258 3.7335701408634616 6 0.875895067594613 2.8084834322256422 7 0.5286025801743262 1.9774060282315578 8 0.364116424866065 1.5566780153942206 9 0.2642361547143166 1.2708763129837048 >10 1.3022934428533925 12.348561591531423 >50 0.07775751884769055 2.802740901244374 >100 0.031225103417229786 3.0548350159351685 >500 7.80610379989465E-4 0.2806097547698728 >1k 3.903051899947325E-4 0.6057861232185089 >5k 1.3010172999824415E-4 0.4291883092652192 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6173 0.4297893523917607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4531 0.31546663788871987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.20044768273706123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.481203243088941E-5 0.0 16-17 0.0 0.0 0.0 1.3924812972355765E-4 0.0 18-19 0.0 0.0 0.0 1.7406016215444707E-4 0.0 20-21 0.0 0.0 0.0 2.436842270162259E-4 0.0 22-23 0.0 0.0 0.0 2.784962594471153E-4 0.0 24-25 0.0 0.0 0.0 3.8293235673978355E-4 0.0 26-27 0.0 0.0 0.0 4.1774438917067296E-4 0.0 28-29 0.0 0.0 0.0 9.747369080649036E-4 0.0 30-31 0.0 0.0 0.0 0.002610902432316706 0.0 32-33 0.0 0.0 0.0 0.006126917707836537 0.0 34-35 0.0 0.0 0.0 0.011905715091364179 0.0 36-37 0.0 0.0 0.0 0.021757520269305886 0.0 38-39 0.0 0.0 0.0 0.038919852257734366 0.0 40-41 0.0 0.0 0.0 0.06025962813786957 0.0 42-43 0.0 0.0 0.0 0.08065947914237076 0.0 44-45 0.0 0.0 0.0 0.10454053338996092 0.0 46-47 0.0 0.0 0.0 0.13312121201572114 0.0 48-49 0.0 0.0 0.0 0.16591414656561893 0.0 50-51 0.0 0.0 0.0 0.20291933703965437 0.0 52-53 0.0 0.0 0.0 0.23787061760026734 0.0 54-55 0.0 0.0 0.0 0.27637272546883107 0.0 56-57 0.0 0.0 0.0 0.3230556609586538 0.0 58-59 0.0 0.0 0.0 0.3764573187076381 0.0 60-61 0.0 0.0 0.0 0.4348719091266705 0.0 62-63 0.0 0.0 0.0 0.49746394343740974 0.0 64-65 0.0 0.0 0.0 0.5579672558022954 0.0 66-67 0.0 0.0 0.0 0.6272432003397654 0.0 68-69 0.0 0.0 0.0 0.7111401984982089 0.0 70-71 0.0 0.0 0.0 0.8070125358128784 0.0 72-73 0.0 0.0 0.0 0.9053913394625719 0.0 74-75 0.0 3.481203243088941E-5 0.0 1.0028302182366313 0.0 76-77 0.0 6.962406486177883E-5 0.0 1.1141242859181848 0.0 78-79 0.0 6.962406486177883E-5 0.0 1.2467233174474426 0.0 80-81 0.0 6.962406486177883E-5 0.0 1.4015672377000388 0.0 82-83 0.0 6.962406486177883E-5 0.0 1.5583606317687644 0.0 84-85 0.0 6.962406486177883E-5 0.0 1.7110113939782146 0.0 86-87 0.0 6.962406486177883E-5 0.0 1.879640879073443 0.0 88-89 0.0 6.962406486177883E-5 0.0 2.06936645582179 0.0 90-91 0.0 6.962406486177883E-5 0.0 2.2903532376930764 0.0 92-93 0.0 6.962406486177883E-5 0.0 2.526448441639368 0.0 94-95 0.0 6.962406486177883E-5 0.0 2.756068607553515 0.0 96-97 0.0 6.962406486177883E-5 0.0 2.9957494508401883 0.0 98-99 0.0 6.962406486177883E-5 0.0 3.2599727769906393 0.0 100-101 0.0 6.962406486177883E-5 0.0 3.568477008393181 0.0 102-103 0.0 6.962406486177883E-5 0.0 3.8964063538921594 0.0 104-105 0.0 6.962406486177883E-5 0.0 4.232829835304274 0.0 106-107 0.0 6.962406486177883E-5 0.0 4.564205572013911 0.0 108-109 0.0 6.962406486177883E-5 0.0 4.915841911598324 0.0 110-111 0.0 6.962406486177883E-5 0.0 5.31917411934261 0.0 112-113 0.0 6.962406486177883E-5 0.0 5.748824223604647 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2595 0.0 68.67605 1 GTATCAA 5625 0.0 62.729324 1 ATCAACG 7030 0.0 49.846294 3 TCAACGC 7105 0.0 49.320118 4 CAACGCA 7160 0.0 48.88501 5 TATCAAC 7400 0.0 47.676117 2 AACGCAG 7445 0.0 47.152214 6 GTGGTAT 1095 0.0 41.36861 1 ACGCAGA 8790 0.0 39.866764 7 CGCAGAG 8900 0.0 39.44088 8 GCAGAGT 10105 0.0 34.502125 9 GTACATG 7215 0.0 33.126804 1 TACATGG 7400 0.0 32.133057 2 TGGTATC 1315 0.0 31.723654 2 ACATGGG 7270 0.0 30.986197 3 CATGGGG 3285 0.0 27.93806 4 AGAGTAC 9660 0.0 27.376581 10-11 CAGAGTA 9785 0.0 26.418825 10-11 AGTACTT 6740 0.0 25.95207 12-13 GAGTACT 6515 0.0 25.935133 12-13 >>END_MODULE