FastQCFastQC Report
Fri 27 May 2016
SRR940074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905860
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24470.27013004216987174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA250.00202843471.6495741
GGTATCA14800.057.690821
GTATCAA29000.051.8841741
GTCGAGT350.007688916851.178271
TCAACGC34200.042.0306474
CAACGCA34550.041.7405175
ATCAACG35200.041.0037733
AACGCAG34500.040.5918856
TATCAAC38700.038.4103742
GTACATG72900.037.184391
CATTCCG2650.035.9643679
TACATGG75900.034.528912
ACACCGA700.002767550834.0527736
ACGCAGA42400.033.0287747
ACATGGG75500.031.993313
CATATAG3950.031.7083243
CGCAGAG43550.031.6092598
GCAGAGT44800.030.048919
CATGGGA57000.029.5086544
CATGGGG15950.029.1681634
Produced by FastQC (version 0.11.5)