FastQCFastQC Report
Fri 27 May 2016
SRR940074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905860
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95351.0525909080873423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69030.7620382840615548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.47623253041308816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.18093303600997945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.13092530854657453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.087.043081
GTATCAA69150.062.02691
TCACGCA401.752077E-459.6347244
ATCAACG89500.047.707783
TCAACGC89500.047.707784
TATCAAC89900.047.4291732
CAACGCA91150.046.8925
AACGCAG93300.045.9850586
TATCACG653.4728866E-545.8728642
TGGTATC9450.039.756482
ACGCAGA109000.039.1345067
CGCAGAG110300.038.4035768
GCAGAGT117100.035.8686529
GTACATG65700.034.5941771
TAACGCA1407.903691E-834.0769844
AGTACGC700.002781946734.0167585
GTGGTAT11750.034.015771
CGACGAA350.002790975933.99610586-87
ACATGGG64700.033.8268053
TACATGG67300.033.4946862
Produced by FastQC (version 0.11.5)