##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 905860 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.12595765350054 34.0 33.0 34.0 31.0 34.0 2 33.15676263440267 34.0 33.0 34.0 31.0 34.0 3 33.20948932506127 34.0 33.0 34.0 31.0 34.0 4 36.447798776852935 37.0 37.0 37.0 35.0 37.0 5 36.480225421146756 37.0 37.0 37.0 35.0 37.0 6 36.57047667409975 37.0 37.0 37.0 35.0 37.0 7 36.560715783895965 37.0 37.0 37.0 35.0 37.0 8 36.56627845362418 37.0 37.0 37.0 35.0 37.0 9 38.431263109089706 39.0 39.0 39.0 37.0 39.0 10-11 38.421753361446584 39.0 39.0 39.0 37.0 39.0 12-13 38.410921665599545 39.0 39.0 39.0 37.0 39.0 14-15 39.9541408164617 41.0 40.0 41.0 38.0 41.0 16-17 39.972966021239486 41.0 40.0 41.0 38.0 41.0 18-19 40.08590786655775 41.0 40.0 41.0 38.0 41.0 20-21 40.09567979599497 41.0 40.0 41.0 38.0 41.0 22-23 40.03280584196233 41.0 40.0 41.0 38.0 41.0 24-25 39.99633552646104 41.0 40.0 41.0 38.0 41.0 26-27 39.887005718322925 41.0 40.0 41.0 37.0 41.0 28-29 39.77920760382399 41.0 40.0 41.0 37.0 41.0 30-31 39.536443821341045 41.0 39.5 41.0 36.5 41.0 32-33 39.24686209789592 41.0 39.0 41.0 35.0 41.0 34-35 39.07538471728523 41.0 39.0 41.0 35.0 41.0 36-37 38.92187976066942 41.0 39.0 41.0 35.0 41.0 38-39 38.75985913938136 41.0 39.0 41.0 35.0 41.0 40-41 38.579814761662945 41.0 39.0 41.0 35.0 41.0 42-43 38.32943225222441 41.0 39.0 41.0 35.0 41.0 44-45 37.95451504647517 41.0 38.0 41.0 34.5 41.0 46-47 37.38435851014505 41.0 38.0 41.0 33.0 41.0 48-49 36.85502781886825 41.0 38.0 41.0 31.0 41.0 50-51 36.76491400437153 41.0 38.0 41.0 31.0 41.0 52-53 36.65090797695008 41.0 38.0 41.0 30.0 41.0 54-55 36.499554014969206 41.0 38.0 41.0 30.0 41.0 56-57 36.335386814739586 41.0 37.0 41.0 29.5 41.0 58-59 36.15058563133376 40.5 37.0 41.0 29.0 41.0 60-61 35.950649658887684 40.0 36.5 41.0 28.0 41.0 62-63 35.70414909588678 40.0 36.0 41.0 28.0 41.0 64-65 35.4293014373082 39.0 35.0 41.0 27.0 41.0 66-67 35.12519594639348 39.0 35.0 41.0 26.0 41.0 68-69 34.76753692623584 38.5 35.0 41.0 26.0 41.0 70-71 34.40282493983618 37.0 35.0 40.5 26.0 41.0 72-73 34.03400028702007 37.0 35.0 39.0 25.0 41.0 74-75 33.651340162939086 36.0 35.0 39.0 24.5 41.0 76-77 33.28678217384585 36.0 35.0 39.0 24.0 41.0 78-79 32.9345395535734 35.5 35.0 37.0 22.5 39.5 80-81 32.60593524385666 35.0 35.0 37.0 22.0 39.0 82-83 32.29424469564833 35.0 35.0 36.5 20.5 39.0 84-85 32.013820016338066 35.0 34.5 36.0 20.0 37.0 86-87 31.799809573223236 35.0 34.0 36.0 19.5 37.0 88-89 31.667850440465415 35.0 34.5 36.0 19.5 37.0 90-91 31.62685183140883 35.0 35.0 35.0 19.5 36.0 92-93 31.526439516040007 35.0 35.0 35.0 19.0 36.0 94-95 31.435012584726117 35.0 35.0 35.0 18.0 36.0 96-97 31.357518269931337 35.0 35.0 35.0 17.5 36.0 98-99 31.299847106616916 35.0 34.0 35.0 16.0 36.0 100-101 31.248647693904136 35.0 34.0 35.0 11.5 35.5 102-103 30.803697591239263 35.0 33.5 35.0 9.0 35.0 104-105 31.13908219813216 35.0 34.0 35.0 7.0 35.0 106-107 31.172215353365864 35.0 34.0 35.0 6.0 35.0 108-109 31.15942474554567 35.0 34.0 35.0 2.0 35.0 110-111 31.11084715077385 35.0 34.0 35.0 2.0 35.0 112-113 31.066940255668648 35.0 34.0 35.0 2.0 35.0 114-115 31.003682136312456 35.0 34.0 35.0 2.0 35.0 116-117 30.93685668867154 35.0 34.0 35.0 2.0 35.0 118-119 30.876371072792708 35.0 34.0 35.0 2.0 35.0 120-121 30.788261431126223 35.0 34.0 35.0 2.0 35.0 122-123 30.685022520036206 35.0 34.0 35.0 2.0 35.0 124-125 29.717767094252977 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17537563845518633 1101 2 0.16057187369274573 1101 3 0.1728128149334225 1101 4 0.06302317350251485 1101 5 0.05952346024778876 1101 6 0.05485176135805858 1101 7 0.1172722574761238 1101 8 0.11879953994341008 1101 9 0.16094442329739422 1101 10-11 0.1585129893946231 1101 12-13 0.16603120741991262 1101 14-15 0.2553270756752397 1101 16-17 0.244481085816048 1101 18-19 0.199869427039431 1101 20-21 0.2195235051922495 1101 22-23 0.24749319868123365 1101 24-25 0.1505174061328063 1101 26-27 0.23949396378918664 1101 28-29 0.32311799278551234 1101 30-31 0.3202524252752781 1101 32-33 0.39648218047396 1101 34-35 0.40051723818617546 1101 36-37 0.45854588394683304 1101 38-39 0.44510646802432774 1101 40-41 0.45475013935298136 1101 42-43 0.4918122655975523 1101 44-45 0.6318892063819277 1101 46-47 0.5342865958454652 1101 48-49 0.48933136394722965 1101 50-51 0.6255531955761526 1101 52-53 0.6272127715611333 1101 54-55 0.683331090095578 1101 56-57 0.6076503897004599 1101 58-59 0.5781352529691546 1101 60-61 0.471149741891459 1101 62-63 0.4101228704222848 1101 64-65 0.4657953746436263 1101 66-67 0.5678074222962266 1101 68-69 0.5314592535529528 1101 70-71 0.6478460801645696 1101 72-73 0.5512562110279831 1101 74-75 0.5546553866445123 1101 76-77 0.5458249391850458 1101 78-79 0.5798130052217232 1101 80-81 0.5622787889852034 1101 82-83 0.5409790799561023 1101 84-85 0.5291348881891267 1101 86-87 0.5277252801232528 1101 88-89 0.4889208899404274 1101 90-91 0.520631444976889 1101 92-93 0.46936534686192033 1101 94-95 0.47878790369326296 1101 96-97 0.4377980018605818 1101 98-99 0.38071386931293816 1101 100-101 0.3836966746854955 1101 102-103 0.337538285662923 1101 104-105 0.36257961127903826 1101 106-107 0.40478053801984615 1101 108-109 0.3580193421794462 1101 110-111 0.3611934459948749 1101 112-113 0.4433909541143102 1101 114-115 0.36454576319853516 1101 116-117 0.32435297152891707 1101 118-119 0.34543117711565685 1101 120-121 0.43306483092383274 1101 122-123 0.39695821901135986 1101 124-125 0.410433943221129 1102 1 0.13220972186352498 1102 2 0.1394377602814032 1102 3 0.10993852750467425 1102 4 0.10985985717087488 1102 5 0.09790089799156476 1102 6 0.12594287046896824 1102 7 0.150413971647545 1102 8 0.10996185617503329 1102 9 0.21642757161222903 1102 10-11 0.19270760885657268 1102 12-13 0.1980372080199686 1102 14-15 0.2389526382315026 1102 16-17 0.27295633334080094 1102 18-19 0.2697156116578867 1102 20-21 0.27151550439216265 1102 22-23 0.2519556449258573 1102 24-25 0.22866562095429543 1102 26-27 0.24501091548435738 1102 28-29 0.250197900776314 1102 30-31 0.06379077911066133 1102 32-33 0.16231476373228304 1102 34-35 0.2359607825406158 1102 36-37 0.1711095403124645 1102 38-39 0.22984134151167268 1102 40-41 0.1772527896180094 1102 42-43 0.06636452082307898 1102 44-45 0.15119873733502232 1102 46-47 0.10875324251013296 1102 48-49 0.027799610771907624 1102 50-51 -0.10325008475188469 1102 52-53 -0.11619036875289623 1102 54-55 -0.12858410142357712 1102 56-57 -0.26128530386889537 1102 58-59 -0.35335029558569886 1102 60-61 -0.27435560864359587 1102 62-63 -0.32588032989775684 1102 64-65 -0.4055905639502342 1102 66-67 -0.3871796764136377 1102 68-69 -0.34079357172957714 1102 70-71 -0.4099108955330024 1102 72-73 -0.28829894448756477 1102 74-75 -0.2735846373578852 1102 76-77 -0.35336137944681667 1102 78-79 -0.3531466907478773 1102 80-81 -0.31926856575026363 1102 82-83 -0.3772735453064229 1102 84-85 -0.4282224475444494 1102 86-87 -0.358066099014664 1102 88-89 -0.3565200541539788 1102 90-91 -0.38113073124072727 1102 92-93 -0.3435159412668938 1102 94-95 -0.2966240375008553 1102 96-97 -0.257806758615466 1102 98-99 -0.3066504671207042 1102 100-101 -0.31780227520949467 1102 102-103 -0.33130699886553217 1102 104-105 -0.3288507317552636 1102 106-107 -0.311045444578415 1102 108-109 -0.36251831158593006 1102 110-111 -0.41662353570329813 1102 112-113 -0.36249683466456517 1102 114-115 -0.33811570294808035 1102 116-117 -0.2840754713153686 1102 118-119 -0.28577794379643606 1102 120-121 -0.23944401996125464 1102 122-123 -0.23838211502204132 1102 124-125 -0.308504750648261 1103 1 0.1028645786689637 1103 2 0.1232464405294067 1103 3 0.15919745871223512 1103 4 0.0050985147833202404 1103 5 0.09578457085841308 1103 6 0.10126419711004786 1103 7 0.1398661909172958 1103 8 0.12519185322256732 1103 9 0.07765540351853417 1103 10-11 0.16075524196354252 1103 12-13 0.11074343772177286 1103 14-15 0.17791455142901924 1103 16-17 0.14886067346481013 1103 18-19 0.1337163989819743 1103 20-21 0.04818591084822543 1103 22-23 0.12724547860364765 1103 24-25 0.024014897600295626 1103 26-27 0.09250663440965923 1103 28-29 0.10733715872336802 1103 30-31 0.18509183215662262 1103 32-33 0.08413938729296433 1103 34-35 0.08062174110768439 1103 36-37 0.12148489706869725 1103 38-39 0.13667177158745858 1103 40-41 0.193436629791222 1103 42-43 0.1622338250254174 1103 44-45 0.1648525115698476 1103 46-47 0.16625860615544497 1103 48-49 0.26017673900732063 1103 50-51 0.18924632308199563 1103 52-53 0.14442689332760494 1103 54-55 0.17382314195798187 1103 56-57 0.15466115809351066 1103 58-59 0.28681661711974016 1103 60-61 0.21741803568274776 1103 62-63 0.18552051647563417 1103 64-65 0.21250135613637156 1103 66-67 0.14736652966843167 1103 68-69 0.2010708116972495 1103 70-71 0.27738051279294496 1103 72-73 0.23555382590905083 1103 74-75 0.16519186101825767 1103 76-77 0.28582728308639815 1103 78-79 0.21024234576427858 1103 80-81 0.1508316212391989 1103 82-83 0.20309374137795544 1103 84-85 0.1896128048913326 1103 86-87 0.15310327407853563 1103 88-89 0.19142758879455002 1103 90-91 0.19450946165367 1103 92-93 0.2168127665374584 1103 94-95 0.18990445378937082 1103 96-97 0.20320377184413374 1103 98-99 0.18710841487909846 1103 100-101 0.19520156299540403 1103 102-103 0.1584849507457271 1103 104-105 0.23240977410045716 1103 106-107 0.17211723626008535 1103 108-109 0.17043946816015776 1103 110-111 0.17070920713697646 1103 112-113 0.1769587307325402 1103 114-115 0.12401085939814749 1103 116-117 0.1541849361248424 1103 118-119 0.12165636385045175 1103 120-121 0.10703014596729332 1103 122-123 0.15004933857504454 1103 124-125 0.13765724128166568 1104 1 0.057289534070100956 1104 2 0.07031804197154656 1104 3 0.03968031154223439 1104 4 -0.00568396129547466 1104 5 -0.060171871961486545 1104 6 0.009859145899326904 1104 7 0.02101412814206327 1104 8 0.04067077636283045 1104 9 0.09903946519595053 1104 10-11 0.08040205330101458 1104 12-13 0.10074378078992652 1104 14-15 0.1623610889357252 1104 16-17 0.19369929108728456 1104 18-19 0.19775825641532663 1104 20-21 0.16147364210734594 1104 22-23 0.1603457075236676 1104 24-25 0.21247782752706712 1104 26-27 0.19891701877074297 1104 28-29 0.19359852675440692 1104 30-31 0.23059046612161893 1104 32-33 0.3059415242956618 1104 34-35 0.3888754930257434 1104 36-37 0.30539905517944277 1104 38-39 0.3006879768794377 1104 40-41 0.3067613968167535 1104 42-43 0.33234182911728993 1104 44-45 0.1942370784930887 1104 46-47 0.4069836806948004 1104 48-49 0.43050461859664324 1104 50-51 0.43493497002119597 1104 52-53 0.35984523375100963 1104 54-55 0.3055258359786137 1104 56-57 0.357913444174919 1104 58-59 0.4248699704549921 1104 60-61 0.3769069109182155 1104 62-63 0.4091740519488809 1104 64-65 0.34442782415617756 1104 66-67 0.3348778353234394 1104 68-69 0.3121363969715176 1104 70-71 0.39851163967826153 1104 72-73 0.4437053591118101 1104 74-75 0.32812428282990425 1104 76-77 0.28959284590529677 1104 78-79 0.2897292873428938 1104 80-81 0.28376273315426914 1104 82-83 0.3578896002334844 1104 84-85 0.35190431301892744 1104 86-87 0.2817253720338506 1104 88-89 0.3800472384907785 1104 90-91 0.4265003329220214 1104 92-93 0.375723104116517 1104 94-95 0.33854262916581135 1104 96-97 0.3588943368461912 1104 98-99 0.35520947028148697 1104 100-101 0.2900310581238372 1104 102-103 0.23157289158830352 1104 104-105 0.2599023981351891 1104 106-107 0.3023782643417725 1104 108-109 0.23779037386007928 1104 110-111 0.2572508774422424 1104 112-113 0.3147695972603195 1104 114-115 0.3713142657530142 1104 116-117 0.3524245286846295 1104 118-119 0.2816575022129548 1104 120-121 0.2527132570340491 1104 122-123 0.3682266486712287 1104 124-125 0.3245026828415689 1105 1 0.13805369349473295 1105 2 0.16922499432395455 1105 3 0.11391015871035393 1105 4 0.0669284150905014 1105 5 0.08695054338163999 1105 6 0.10996178299851778 1105 7 0.09904754138749183 1105 8 0.08947958667148725 1105 9 0.14372780801884488 1105 10-11 0.18106694691803682 1105 12-13 0.22149820092776906 1105 14-15 0.3232930637634155 1105 16-17 0.2505733546174014 1105 18-19 0.18181017430563884 1105 20-21 0.21790793938034625 1105 22-23 0.17261758346013067 1105 24-25 0.11025427343656702 1105 26-27 0.10972013534251346 1105 28-29 0.14498749888505813 1105 30-31 0.13835342686480345 1105 32-33 0.11167646585994362 1105 34-35 0.0676865508621276 1105 36-37 0.1510433464590406 1105 38-39 0.20145363465589128 1105 40-41 0.16588635935796248 1105 42-43 0.3284543553384438 1105 44-45 0.2751892810702472 1105 46-47 0.27645064203732517 1105 48-49 0.15326533181210067 1105 50-51 0.19705251572093374 1105 52-53 0.2039893002778328 1105 54-55 0.12990289621235718 1105 56-57 0.16641209565828774 1105 58-59 0.1418459219083843 1105 60-61 0.2032235875738948 1105 62-63 0.3950936653741195 1105 64-65 0.39085388049421255 1105 66-67 0.38944443706152754 1105 68-69 0.36713077815221595 1105 70-71 0.33936570021167967 1105 72-73 0.33818805314837164 1105 74-75 0.2934224548406945 1105 76-77 0.33327691842552554 1105 78-79 0.2992599286374684 1105 80-81 0.2860836801126183 1105 82-83 0.32947822774322333 1105 84-85 0.2966286162853393 1105 86-87 0.3388511586685503 1105 88-89 0.35106387019590457 1105 90-91 0.24076052171435336 1105 92-93 0.3389426875747148 1105 94-95 0.34051071427219526 1105 96-97 0.3873090806280324 1105 98-99 0.42860248796203493 1105 100-101 0.450935313442983 1105 102-103 0.49140694912501104 1105 104-105 0.40288449283102423 1105 106-107 0.42694037102442195 1105 108-109 0.5229001281303809 1105 110-111 0.5052771735165607 1105 112-113 0.5013896901581312 1105 114-115 0.5631703490613766 1105 116-117 0.6024777201739937 1105 118-119 0.6607468788986033 1105 120-121 0.6570382495422891 1105 122-123 0.5858637478621205 1105 124-125 0.5844408758292801 1106 1 0.14624635190016022 1106 2 0.09331772646905989 1106 3 0.12252798932490805 1106 4 0.008426589583763189 1106 5 0.020432315292211456 1106 6 0.04690464373843639 1106 7 0.11476524430650414 1106 8 0.13643917372927916 1106 9 0.06531195218891384 1106 10-11 0.10916895947176641 1106 12-13 0.11008905361028098 1106 14-15 0.16479879207765435 1106 16-17 0.20617019640081935 1106 18-19 0.18655829034672422 1106 20-21 0.23705471731599204 1106 22-23 0.2859220204306965 1106 24-25 0.24748669731383188 1106 26-27 0.2696758915339501 1106 28-29 0.22973993139527238 1106 30-31 0.34232745610887605 1106 32-33 0.3562961669444604 1106 34-35 0.29599647334553225 1106 36-37 0.45164189684482636 1106 38-39 0.4464664941368284 1106 40-41 0.4246640791831453 1106 42-43 0.5806742794405224 1106 44-45 0.44960880589894003 1106 46-47 0.543150161830134 1106 48-49 0.718526388267918 1106 50-51 0.6303191835866144 1106 52-53 0.5293589878733798 1106 54-55 0.5375678662668548 1106 56-57 0.5377439062372318 1106 58-59 0.407725312566825 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2216 80-81 -0.22478222785373703 2216 82-83 -0.2817439395007142 2216 84-85 -0.3109221011812622 2216 86-87 -0.31690341827355795 2216 88-89 -0.3781592866571408 2216 90-91 -0.27816080436184265 2216 92-93 -0.1825120378087597 2216 94-95 -0.22234925603953215 2216 96-97 -0.20698087483955874 2216 98-99 -0.2365632715629502 2216 100-101 -0.23288457770001258 2216 102-103 -0.31353120030596315 2216 104-105 -0.2907325284455702 2216 106-107 -0.25591959928730645 2216 108-109 -0.2539628110086589 2216 110-111 -0.26887423667641386 2216 112-113 -0.2736028877735883 2216 114-115 -0.34506319868726365 2216 116-117 -0.4175222798260094 2216 118-119 -0.3721180394586483 2216 120-121 -0.3693645213632024 2216 122-123 -0.5197968162150701 2216 124-125 -0.7730851014097126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 5.0 4 21.0 5 61.0 6 166.0 7 394.0 8 717.0 9 1282.0 10 2946.0 11 4440.0 12 5359.0 13 8735.0 14 17373.0 15 18405.0 16 6470.0 17 3895.0 18 3171.0 19 2722.0 20 2551.0 21 2544.0 22 2430.0 23 2037.0 24 2233.0 25 2560.0 26 3004.0 27 3689.0 28 4518.0 29 5398.0 30 6757.0 31 8679.0 32 10885.0 33 14861.0 34 20813.0 35 36966.0 36 81719.0 37 328632.0 38 287240.0 39 2173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36996667822293 20.48947018595274 14.467922545085976 20.672640590738357 2 15.036540669433895 21.603373664223263 42.12002119579616 21.240064470546674 3 17.60610958033065 29.402024162780837 31.28687120248693 21.704995054401582 4 10.91242534083711 18.464732354570085 40.71893885880229 29.903903445790508 5 10.90439013938756 40.008459779051776 34.89625726563596 14.190892815924705 6 26.588100726922637 40.766293931123414 18.49689092627432 14.148714415679626 7 24.994369990947828 36.23109531274149 22.494977148786788 16.2795575475239 8 25.45117347051421 34.782416708983725 20.88435299052834 18.882056829973727 9 27.83266729958272 15.343761729185527 21.854922394188947 34.96864857704281 10-11 23.3197734749299 28.52195703530347 28.111904709336983 20.046364780429645 12-13 25.19191707327843 26.950190978738437 28.91500894177908 18.94288300620405 14-15 22.58748592497737 26.52054401342371 29.1456185282494 21.746351533349525 16-17 19.177356324376834 30.086768374804052 29.617987326960016 21.117887973859094 18-19 19.038206786920718 26.820038416532356 34.95518071225134 19.18657408429559 20-21 20.4663524164882 25.990660808513454 35.10150575144062 18.441481023557724 22-23 20.051222043141323 26.217020290111055 34.82684962356214 18.904908043185483 24-25 19.273232066765285 26.535005409224382 35.31058883271146 18.881173691298876 26-27 19.23095180270682 26.685083787781778 35.10663899498819 18.977325414523214 28-29 19.097805616332803 26.2918139487646 35.51903278438784 19.091347650514763 30-31 19.50038582163449 26.203305370924173 35.33309267777877 18.963216129662563 32-33 19.328415711954854 26.31621070852164 35.02221098671094 19.33316259281256 34-35 19.596226992053367 26.309328584967467 34.96832552211694 19.126118900862224 36-37 19.82139417198994 26.007355526497506 34.651285892413696 19.51996440909886 38-39 19.26691947208929 26.683612357658955 34.45340486954026 19.596063300711496 40-41 19.675071023707318 26.22465183618431 34.692686890185506 19.407590249922862 42-43 19.634011849657064 26.280980604935262 34.07096546996243 20.014042075445246 44-45 19.93588353057869 26.496064752335236 33.66009770917198 19.9079540079141 46-47 20.43927366179505 26.826194805151793 32.48320102797455 20.251330505078606 48-49 20.413625532445725 27.305102923407855 31.76547604525506 20.515795498891364 50-51 20.428121018897336 27.55022399049724 31.159462687753493 20.86219230285193 52-53 20.390424999917204 27.92145355954955 31.056323335088575 20.631798105444666 54-55 20.67146137372221 27.759532378071665 30.890148588082045 20.67885766012408 56-57 20.77203944372535 28.00636972558847 30.409423219692055 20.812167610994127 58-59 20.625636145658312 27.510912450902254 31.002068784318748 20.86138261912069 60-61 20.684361997723688 28.058863985357412 30.62700157089853 20.62977244602037 62-63 20.490904755491478 27.87903843648784 31.06491304376624 20.565143764254444 64-65 20.404958458722565 27.600276426677382 30.928380762292928 21.066384352307125 66-67 20.498315123047075 27.998266826000922 30.374591889960012 21.12882616099199 68-69 20.743445382789645 27.558094607274935 30.716674946183144 20.981785063752277 70-71 20.712228837888098 27.473363312658382 31.00685381022061 20.80755403923292 72-73 20.94098425805312 27.367584394939616 30.655564877574903 21.035866469432364 74-75 20.55546172196777 28.103214738717615 30.159991786801253 21.181331752513362 76-77 20.502472897485152 28.061093815548343 30.51947406769557 20.916959219270936 78-79 20.562630540088225 27.765407594176533 30.21873330301197 21.453228562723268 80-81 20.395756518667344 28.029552027907183 30.01589649614731 21.558794957278167 82-83 20.800841189587796 28.077959066522425 29.93017684851964 21.191022895370146 84-85 20.546995526338304 27.752267878777843 29.948363843098942 21.75237275178491 86-87 20.375003794746966 27.996346003648476 30.14348283256472 21.485167369039832 88-89 20.620570507583956 27.624577749321087 30.14301326915859 21.61183847393637 90-91 20.408607088528225 27.817660163071917 30.31280840926503 21.46092433913483 92-93 20.558986018174 27.69953673575613 30.20280850843044 21.538668737639433 94-95 20.320781232233557 27.576775871528543 30.27434975509217 21.82809314114573 96-97 20.820065474091038 27.449772562812242 29.91810494952556 21.812057013571167 98-99 20.279237409754266 27.505795597553707 29.869957830128275 22.345009162563752 100-101 20.108957534711585 27.75453092787773 30.06543523677841 22.071076300632274 102-103 20.599595964056256 27.346444262910385 29.87790607820193 22.17605369483143 104-105 20.375554721480142 27.27043914070607 29.982833992007595 22.371172145806195 106-107 20.140651339008254 27.569362493197048 29.703584561360124 22.58640160643457 108-109 20.311020907860026 27.549703015547184 29.303017975825064 22.83625810076773 110-111 20.256938158214293 27.567615304793236 29.35569514052944 22.81975139646303 112-113 20.234562020448006 27.66701421910817 29.565820710408964 22.532603050034854 114-115 19.87757253606449 27.902091147191705 29.211479920626378 23.008856396117427 116-117 19.910327960333674 27.843814114045657 29.262581876930838 22.98327604868983 118-119 19.89243335316722 27.694713403992022 29.242584646362506 23.17026859647825 120-121 19.618660509714807 28.10204010666113 29.080227121314067 23.199072262309993 122-123 19.808501571993784 28.10518448848382 28.855358909142293 23.230955030380105 124-125 19.558316613355927 28.16937636094177 28.95554187905868 23.316765146643633 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 267.0 1 217.5 2 371.0 3 524.0 4 300.0 5 204.0 6 522.0 7 1306.0 8 2360.0 9 2719.5 10 2020.0 11 1362.5 12 1282.5 13 1418.5 14 1613.0 15 1747.5 16 1945.0 17 2145.0 18 2354.5 19 2593.5 20 2783.5 21 3206.5 22 3761.0 23 4469.5 24 5253.5 25 6113.0 26 8169.5 27 10290.0 28 11152.5 29 12599.0 30 15489.0 31 18406.5 32 21888.0 33 25360.5 34 28903.0 35 31584.5 36 35402.0 37 39765.5 38 37957.0 39 34551.0 40 33266.5 41 32601.5 42 31226.0 43 30803.5 44 32185.0 45 32651.5 46 30956.0 47 28380.0 48 27222.5 49 26388.5 50 24999.5 51 22565.5 52 20716.0 53 20417.5 54 19734.0 55 18376.5 56 16306.5 57 13743.0 58 12111.5 59 10661.5 60 8860.5 61 7224.0 62 5586.0 63 4025.0 64 2783.5 65 2006.5 66 1451.5 67 1131.5 68 878.5 69 640.0 70 461.5 71 334.0 72 241.0 73 224.0 74 166.0 75 124.5 76 95.5 77 33.5 78 13.0 79 8.0 80 5.5 81 3.0 82 1.0 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016117280816020138 2 0.002207846687126046 3 4.415693374252092E-4 4 0.13788002561102156 5 0.044046541408164615 6 0.013688649460181484 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.207846687126046E-4 30-31 1.103923343563023E-4 32-33 2.207846687126046E-4 34-35 4.967655046033603E-4 36-37 0.001159119510741174 38-39 0.0027598083589075576 40-41 5.519616717815115E-5 42-43 0.0012143156779193252 44-45 9.383348420285696E-4 46-47 1.103923343563023E-4 48-49 0.0018214735168789878 50-51 0.0019870620184134413 52-53 1.103923343563023E-4 54-55 0.0 56-57 0.0013799041794537788 58-59 0.001545492680988232 60-61 9.935310092067206E-4 62-63 2.207846687126046E-4 64-65 0.001545492680988232 66-67 8.279425076722673E-4 68-69 0.001103923343563023 70-71 0.001490296513810081 72-73 0.0 74-75 1.103923343563023E-4 76-77 0.004415693374252092 78-79 8.831386748504184E-4 80-81 0.0 82-83 0.0 84-85 2.7598083589075575E-4 86-87 2.7598083589075575E-4 88-89 0.0 90-91 2.207846687126046E-4 92-93 6.071578389596626E-4 94-95 9.935310092067206E-4 96-97 6.071578389596626E-4 98-99 0.0 100-101 2.7598083589075575E-4 102-103 0.0 104-105 0.0 106-107 3.311770030689069E-4 108-109 4.967655046033603E-4 110-111 0.0 112-113 1.6558850153445344E-4 114-115 0.0019318658512352902 116-117 3.86373170247058E-4 118-119 3.311770030689069E-4 120-121 5.519616717815115E-5 122-123 4.415693374252092E-4 124-125 8.279425076722673E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 905860.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.72319754876171 #Duplication Level Percentage of deduplicated Percentage of total 1 64.72792970703188 22.475606901396294 2 14.538805176592978 10.096676085395947 3 6.639678317338656 6.916525856195396 4 3.693758708511626 5.130364533324324 5 2.396400361913623 4.160534158632539 6 1.5857192341975583 3.303674533554777 7 1.1421869404637632 2.776226793993729 8 0.8679044678786481 2.410913463328257 9 0.6448589783730587 2.015240912742634 >10 3.340663316284305 20.844728848174128 >50 0.2649625446158542 6.3982687724949985 >100 0.15013445443072038 9.598378000087427 >500 0.005407385011178371 1.2688272860153245 >1k 9.542444137373596E-4 0.7884886833968837 >5k 6.36162942491573E-4 1.815545171267401 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9535 1.0525909080873423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6903 0.7620382840615548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4314 0.47623253041308816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1639 0.18093303600997945 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.13092530854657453 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.207846687126046E-4 0.0 22-23 0.0 0.0 0.0 0.0010487271763848717 0.0 24-25 0.0 0.0 0.0 0.0019318658512352902 0.0 26-27 0.0 0.0 0.0 0.0035325546994016735 0.0 28-29 0.0 0.0 0.0 0.00673393239573444 0.0 30-31 0.0 0.0 0.0 0.015178945973991565 0.0 32-33 0.0 0.0 0.0 0.032786523303821785 0.0 34-35 0.0 0.0 0.0 0.05817676020577131 0.0 36-37 0.0 0.0 0.0 0.09653809639458635 0.0 38-39 0.0 0.0 0.0 0.15438367959728877 0.0 40-41 0.0 0.0 0.0 0.22591791226017266 0.0 42-43 0.0 0.0 0.0 0.2948027288985053 0.0 44-45 0.0 0.0 0.0 0.3660609807254984 0.0 46-47 0.0 0.0 0.0 0.45233259002494863 0.0 48-49 0.0 0.0 0.0 0.5533967721281434 0.0 50-51 0.0 0.0 0.0 0.6592078246086592 0.0 52-53 0.0 0.0 0.0 0.7659572119312035 0.0 54-55 0.0 0.0 0.0 0.873479345594242 0.0 56-57 0.0 0.0 0.0 1.0004305301039895 0.0 58-59 0.0 0.0 0.0 1.1498465546552448 0.0 60-61 0.0 0.0 0.0 1.3210098690746914 0.0 62-63 0.0 0.0 0.0 1.4779877685293532 0.0 64-65 0.0 0.0 0.0 1.6343585101450555 0.0 66-67 0.0 0.0 0.0 1.8172786081734484 0.0 68-69 0.0 0.0 0.0 2.031439736824675 0.0 70-71 0.0 0.0 0.0 2.2781666041110107 0.0 72-73 0.0 0.0 0.0 2.5323449539663967 0.0 74-75 0.0 0.0 0.0 2.773441812200561 0.0 76-77 0.0 0.0 0.0 3.030987128253814 0.0 78-79 0.0 0.0 0.0 3.3437286114852185 0.0 80-81 0.0 0.0 0.0 3.6840129821385204 0.0 82-83 0.0 0.0 0.0 4.037047667409976 0.0 84-85 0.0 0.0 0.0 4.385942640143068 0.0 86-87 0.0 0.0 0.0 4.7514516591967855 0.0 88-89 0.0 0.0 0.0 5.161338396661735 0.0 90-91 0.0 0.0 0.0 5.620570507583953 0.0 92-93 0.0 0.0 0.0 6.113417084317665 0.0 94-95 0.0 0.0 0.0 6.619234760338243 0.0 96-97 0.0 0.0 0.0 7.127646656216192 0.0 98-99 0.0 0.0 0.0 7.669341840902568 0.0 100-101 0.0 0.0 0.0 8.264025346079968 0.0 102-103 0.0 0.0 0.0 8.91031726756894 0.0 104-105 0.0 0.0 0.0 9.579570794604024 0.0 106-107 0.0 0.0 5.519616717815115E-5 10.235080476011746 0.0 108-109 0.0 0.0 1.103923343563023E-4 10.903947629876582 0.0 110-111 0.0 0.0 1.103923343563023E-4 11.671174353652884 0.0 112-113 0.0 0.0 1.103923343563023E-4 12.468207007705384 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3180 0.0 87.04308 1 GTATCAA 6915 0.0 62.0269 1 TCACGCA 40 1.752077E-4 59.634724 4 ATCAACG 8950 0.0 47.70778 3 TCAACGC 8950 0.0 47.70778 4 TATCAAC 8990 0.0 47.429173 2 CAACGCA 9115 0.0 46.892 5 AACGCAG 9330 0.0 45.985058 6 TATCACG 65 3.4728866E-5 45.872864 2 TGGTATC 945 0.0 39.75648 2 ACGCAGA 10900 0.0 39.134506 7 CGCAGAG 11030 0.0 38.403576 8 GCAGAGT 11710 0.0 35.868652 9 GTACATG 6570 0.0 34.594177 1 TAACGCA 140 7.903691E-8 34.076984 4 AGTACGC 70 0.0027819467 34.016758 5 GTGGTAT 1175 0.0 34.01577 1 CGACGAA 35 0.0027909759 33.996105 86-87 ACATGGG 6470 0.0 33.826805 3 TACATGG 6730 0.0 33.494686 2 >>END_MODULE