FastQCFastQC Report
Fri 27 May 2016
SRR940073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756648
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10160.1342764402998488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.053.713321
GGTATCA9100.051.1360171
ATCAACG18000.041.700263
TCAACGC18200.041.577594
CAACGCA18300.041.3339655
AACGCAG19700.038.3965266
TATCAAC21100.036.1910552
GTACATG41150.034.2149471
TACATGG40650.034.1955072
ACGCAGA23200.032.6039477
GTATAGG3000.031.8179661
ACATGGG42800.031.734513
CGCAGAG24550.030.5684558
GTGTACG800.005291696629.8293481
TACGTTA400.00536580629.746513102-103
GCAGAGT26800.027.3245059
CATGGGG19550.026.8202534
CATGGGA25450.026.6897224
TATAGGG1451.3154797E-424.6863542
TAGGGTG2901.4370016E-1024.6455045
Produced by FastQC (version 0.11.5)