##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940073_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 756648 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.500870946596038 34.0 31.0 34.0 30.0 34.0 2 31.500524682547233 34.0 31.0 34.0 28.0 34.0 3 31.67880308941542 34.0 31.0 34.0 29.0 34.0 4 34.8518611031814 37.0 35.0 37.0 33.0 37.0 5 34.981710121483175 37.0 35.0 37.0 33.0 37.0 6 35.015973081274254 37.0 37.0 37.0 33.0 37.0 7 34.958443027669404 37.0 37.0 37.0 33.0 37.0 8 34.90435182541948 37.0 37.0 37.0 32.0 37.0 9 36.506444211839586 39.0 39.0 39.0 33.0 39.0 10-11 36.54537644981551 39.0 39.0 39.0 33.0 39.0 12-13 36.51009782620188 39.0 39.0 39.0 33.0 39.0 14-15 38.00461178777979 41.0 40.0 41.0 33.0 41.0 16-17 38.057276963660776 41.0 40.0 41.0 33.0 41.0 18-19 38.091514151890976 41.0 40.0 41.0 33.0 41.0 20-21 38.14113630115985 41.0 40.0 41.0 33.0 41.0 22-23 38.16114890940041 41.0 39.5 41.0 33.0 41.0 24-25 38.23915678096023 41.0 39.0 41.0 33.0 41.0 26-27 38.29960100337277 41.0 39.0 41.0 33.0 41.0 28-29 38.255304976686645 41.0 39.0 41.0 33.0 41.0 30-31 38.19863133187427 41.0 39.0 41.0 33.0 41.0 32-33 38.16334543935886 41.0 39.0 41.0 33.0 41.0 34-35 38.08269234835749 41.0 39.0 41.0 33.0 41.0 36-37 37.93206352227192 41.0 39.0 41.0 32.0 41.0 38-39 37.83978877893023 41.0 38.5 41.0 32.0 41.0 40-41 37.75107051099058 41.0 38.0 41.0 31.5 41.0 42-43 37.57440778274706 40.5 38.0 41.0 31.0 41.0 44-45 37.405026511667245 40.0 38.0 41.0 31.0 41.0 46-47 37.249004821264315 40.0 38.0 41.0 30.0 41.0 48-49 37.093653852253624 40.0 37.0 41.0 30.0 41.0 50-51 36.92367719203645 40.0 37.0 41.0 30.0 41.0 52-53 37.06276234127362 40.0 37.0 41.0 30.0 41.0 54-55 36.871109815924974 40.0 36.5 41.0 30.0 41.0 56-57 36.76147957835083 40.0 36.0 41.0 30.0 41.0 58-59 36.57677347987439 40.0 35.5 41.0 30.0 41.0 60-61 36.34296661063004 39.0 35.0 41.0 30.0 41.0 62-63 36.06294472462757 39.0 35.0 41.0 30.0 41.0 64-65 35.762620795931525 38.5 35.0 41.0 30.0 41.0 66-67 35.44842515938719 37.5 35.0 40.5 30.0 41.0 68-69 35.10989257884776 37.0 35.0 40.0 29.5 41.0 70-71 34.75520982015415 36.5 35.0 39.5 29.0 41.0 72-73 34.389551680570094 36.0 35.0 39.0 29.0 41.0 74-75 34.02377525612967 35.5 34.0 39.0 29.0 40.5 76-77 33.66634022689547 35.0 34.0 37.0 28.5 39.5 78-79 33.324455361013314 35.0 34.0 37.0 28.0 39.0 80-81 32.99462960319726 35.0 34.0 36.5 28.0 39.0 82-83 32.6858010594094 35.0 34.0 36.0 27.0 37.5 84-85 32.44004741967203 35.0 34.0 36.0 27.0 37.0 86-87 32.22052724648714 35.0 34.0 35.0 27.0 37.0 88-89 32.036553324663515 35.0 34.0 35.0 26.0 36.0 90-91 31.879631612057388 35.0 33.5 35.0 26.0 36.0 92-93 31.72369516604815 35.0 33.0 35.0 25.0 36.0 94-95 31.60176198179338 35.0 33.0 35.0 25.0 36.0 96-97 31.501417435848637 35.0 33.0 35.0 25.0 35.0 98-99 31.42075575432698 35.0 33.0 35.0 25.0 35.0 100-101 31.32360357788562 35.0 33.0 35.0 24.5 35.0 102-103 30.612795513898142 34.0 31.5 35.0 24.0 35.0 104-105 31.513970168427065 35.0 33.5 35.0 25.0 35.0 106-107 31.622801487613792 35.0 34.0 35.0 25.0 35.0 108-109 31.60571031708271 35.0 34.0 35.0 25.0 35.0 110-111 31.550557194362504 35.0 34.0 35.0 25.0 35.0 112-113 31.49160838329051 35.0 34.0 35.0 25.0 35.0 114-115 31.42141392034341 35.0 34.0 35.0 24.5 35.0 116-117 31.34026059673719 35.0 34.0 35.0 24.0 35.0 118-119 31.24755963142703 35.0 34.0 35.0 24.0 35.0 120-121 31.145119659339613 35.0 33.5 35.0 23.0 35.0 122-123 31.011604471299734 35.0 33.0 35.0 20.0 35.0 124-125 29.75550454108119 34.5 31.0 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07841653343966115 1101 2 0.16722895065037946 1101 3 0.15380174838438876 1101 4 0.1592394370506014 1101 5 0.17732211357666472 1101 6 0.15703206090852007 1101 7 0.31736256551292286 1101 8 0.1482042602574012 1101 9 0.19893174834408 1101 10-11 0.0236437918344663 1101 12-13 0.0036466317173733387 1101 14-15 0.10706114878558992 1101 16-17 0.055401297741219935 1101 18-19 0.04929643668945971 1101 20-21 -0.0424189821736789 1101 22-23 0.14006355181146546 1101 24-25 -0.02304800347528868 1101 26-27 0.05663215834521651 1101 28-29 -0.048566691626092506 1101 30-31 0.027572051427597444 1101 32-33 -0.04943156178276098 1101 34-35 -0.08580662855899845 1101 36-37 -0.036359418044249026 1101 38-39 0.009735043849232738 1101 40-41 0.042681233928156814 1101 42-43 0.07642941284780136 1101 44-45 0.13976481289292764 1101 46-47 0.09252503436159287 1101 48-49 -0.02084340452398692 1101 50-51 0.02427641583362572 1101 52-53 0.05531355172691832 1101 54-55 0.05444609801229916 1101 56-57 -0.03816448177391152 1101 58-59 -0.007140215314954901 1101 60-61 -0.08675562467526277 1101 62-63 -0.013291254883945669 1101 64-65 -0.03415766463047021 1101 66-67 -0.01898077992284186 1101 68-69 0.07023621911245215 1101 70-71 0.10365663266997416 1101 72-73 0.014581301688735948 1101 74-75 0.15449121731854376 1101 76-77 0.03702879180493568 1101 78-79 -0.07303994250988666 1101 80-81 0.046163107688741434 1101 82-83 0.13990346187047464 1101 84-85 0.03181418883133347 1101 86-87 -0.023625042816476594 1101 88-89 -0.03224639437678434 1101 90-91 -0.16651321971671607 1101 92-93 -0.11400544879301577 1101 94-95 0.09310344310040364 1101 96-97 -0.05517419994565387 1101 98-99 0.01550515930733809 1101 100-101 0.10668248097272226 1101 102-103 -0.03247616312598467 1101 104-105 0.15159074912682158 1101 106-107 0.19941693066965627 1101 108-109 0.16040931585328977 1101 110-111 0.23019140077603595 1101 112-113 0.27658329051195096 1101 114-115 0.16425513103599343 1101 116-117 0.13071602468523835 1101 118-119 -0.09504166839441197 1101 120-121 0.010983399169681718 1101 122-123 -0.06422187714388627 1101 124-125 -0.1121166980000119 1102 1 0.19519275881758347 1102 2 0.2904126428171203 1102 3 0.24280541306601933 1102 4 0.34937342697273266 1102 5 0.37130974527615734 1102 6 0.26177553319435987 1102 7 0.3593311866764637 1102 8 0.35789963359683696 1102 9 0.39792281568260535 1102 10-11 0.36066852843547537 1102 12-13 0.3620713912226421 1102 14-15 0.5958558120929709 1102 16-17 0.5350285309065868 1102 18-19 0.458355644202058 1102 20-21 0.4809124424713005 1102 22-23 0.4389544130116576 1102 24-25 0.5017825049992908 1102 26-27 0.5172368308142978 1102 28-29 0.5773855529914087 1102 30-31 0.5912710894486679 1102 32-33 0.573236784529648 1102 34-35 0.5781781401079655 1102 36-37 0.5681756254738488 1102 38-39 0.5656243017512068 1102 40-41 0.44241439929691495 1102 42-43 0.5207147999297916 1102 44-45 0.4493376484403413 1102 46-47 0.3718585203899991 1102 48-49 0.3800887988658417 1102 50-51 0.511375591280256 1102 52-53 0.4742691174621356 1102 54-55 0.5470594741002444 1102 56-57 0.5443263565220064 1102 58-59 0.49641910671893896 1102 60-61 0.48092302855424407 1102 62-63 0.46835956966942405 1102 64-65 0.4301534897442423 1102 66-67 0.49225032406250335 1102 68-69 0.4298227972159836 1102 70-71 0.298893691762963 1102 72-73 0.2763563818536454 1102 74-75 0.2977149919406301 1102 76-77 0.25385025309673637 1102 78-79 0.4116777624832437 1102 80-81 0.38928209532044633 1102 82-83 0.3768675021819732 1102 84-85 0.31506659377865276 1102 86-87 0.26552062827835243 1102 88-89 0.3147434132274327 1102 90-91 0.22140707345781507 1102 92-93 0.31693992454642483 1102 94-95 0.30652934781867813 1102 96-97 0.25832671622475445 1102 98-99 0.29034082124045923 1102 100-101 0.3367763884395103 1102 102-103 0.22944206866512573 1102 104-105 0.3593970936068942 1102 106-107 0.40708298655605546 1102 108-109 0.5112539104478842 1102 110-111 0.40739421800004294 1102 112-113 0.41257046412624376 1102 114-115 0.3755217366246306 1102 116-117 0.4273370737003539 1102 118-119 0.40672997613146933 1102 120-121 0.3876353689360599 1102 122-123 0.4234905598693999 1102 124-125 0.409600548907914 1103 1 -0.03419154633822785 1103 2 0.08084898577984845 1103 3 -0.013275139824575177 1103 4 0.05569249388101838 1103 5 -0.017006176978043186 1103 6 0.21178530471836865 1103 7 0.21650925937660048 1103 8 0.15386577082456654 1103 9 0.19515703022973696 1103 10-11 0.11607395784953667 1103 12-13 0.1897183638268416 1103 14-15 0.3314483661916796 1103 16-17 0.34662799613772677 1103 18-19 0.2247573693202014 1103 20-21 0.20991032826884037 1103 22-23 0.16659649985385272 1103 24-25 0.2652538833171647 1103 26-27 0.269448748520297 1103 28-29 0.2753088820608056 1103 30-31 0.1974564641421921 1103 32-33 0.17947545844501178 1103 34-35 0.1038517090238642 1103 36-37 0.2773750711619414 1103 38-39 0.10140029400504602 1103 40-41 0.24929577299553074 1103 42-43 0.23367628235881455 1103 44-45 0.12044813772720175 1103 46-47 0.1607713939287052 1103 48-49 0.07054024327476327 1103 50-51 -0.04830843316632638 1103 52-53 0.16031893446814394 1103 54-55 0.24686493194056425 1103 56-57 0.1308065647443044 1103 58-59 0.2053964723160675 1103 60-61 0.17578276276879023 1103 62-63 0.21442986245981643 1103 64-65 0.22119278015394173 1103 66-67 0.32687394272433323 1103 68-69 0.21315479806877136 1103 70-71 0.13539610799434598 1103 72-73 0.10968527358516411 1103 74-75 0.10257099520963919 1103 76-77 0.16823282602783252 1103 78-79 0.13895563797625954 1103 80-81 0.02843859071894883 1103 82-83 0.1266698509010169 1103 84-85 0.13935682580792985 1103 86-87 0.1292300764537444 1103 88-89 0.06400721772588724 1103 90-91 -0.052328846664419615 1103 92-93 -0.04989530090928085 1103 94-95 0.019052222068047797 1103 96-97 -0.010161451348000838 1103 98-99 -0.03696636882456872 1103 100-101 -0.09920680453920383 1103 102-103 -0.08743735072868475 1103 104-105 0.03959176901463479 1103 106-107 0.10075015068212423 1103 108-109 0.04158671967220329 1103 110-111 0.11464491088992546 1103 112-113 0.10405680175905019 1103 114-115 0.052461828299293245 1103 116-117 0.13073245621110274 1103 118-119 0.132863028755569 1103 120-121 0.1485605990110308 1103 122-123 0.17803320813007062 1103 124-125 0.08067439497975926 1104 1 -0.41414798179425816 1104 2 -0.2504201974710476 1104 3 -0.3392300513825255 1104 4 -0.19940976656779696 1104 5 -0.11468632196359607 1104 6 -0.024746062183773176 1104 7 -0.09729751551265764 1104 8 -0.13896372908718035 1104 9 -0.11780169968300669 1104 10-11 -0.06030648287556062 1104 12-13 -0.025787299133398278 1104 14-15 -3.8044064778119946E-4 1104 16-17 -0.08016893368116484 1104 18-19 0.004497058255580555 1104 20-21 -0.16550239412772783 1104 22-23 -0.2109309539879689 1104 24-25 -0.20195970778740246 1104 26-27 -0.24679755473542286 1104 28-29 -0.1839526730901042 1104 30-31 -0.24301788142106773 1104 32-33 -0.19980727630632344 1104 34-35 -0.1887851512295171 1104 36-37 -0.13949499715518243 1104 38-39 -0.07103802901829681 1104 40-41 -0.22668776546137082 1104 42-43 -0.09119920139272608 1104 44-45 -0.16080791589335064 1104 46-47 -0.25763979165538586 1104 48-49 -0.2841082915876427 1104 50-51 -0.15013947850013665 1104 52-53 -0.09557218000070833 1104 54-55 -0.0958119629233849 1104 56-57 -0.24878826777757723 1104 58-59 -0.26221208389812034 1104 60-61 -0.25810309123833264 1104 62-63 -0.11608275274731028 1104 64-65 -0.2059302380252177 1104 66-67 -0.21340388886339667 1104 68-69 -0.2135076068490278 1104 70-71 -0.22208175458792567 1104 72-73 -0.17694209028639563 1104 74-75 -0.31419683418264555 1104 76-77 -0.42277918866013664 1104 78-79 -0.39379525634527823 1104 80-81 -0.43282684735870447 1104 82-83 -0.369093152814024 1104 84-85 -0.35934958274312123 1104 86-87 -0.2940928823192195 1104 88-89 -0.2829023686261891 1104 90-91 -0.2620962154434494 1104 92-93 -0.41445885908215274 1104 94-95 -0.4190964570050433 1104 96-97 -0.3468202402364575 1104 98-99 -0.2617671643510242 1104 100-101 -0.3498600574589368 1104 102-103 -0.5281646031060063 1104 104-105 -0.36933272054408306 1104 106-107 -0.247151691340445 1104 108-109 -0.28103305053774363 1104 110-111 -0.29470898215309305 1104 112-113 -0.36408877786032434 1104 114-115 -0.3658514231568013 1104 116-117 -0.2845545230699109 1104 118-119 -0.26966345650696155 1104 120-121 -0.20808536158286017 1104 122-123 -0.21151861947143047 1104 124-125 -0.11389704097132025 1105 1 -0.022288543445188935 1105 2 0.12623179414760344 1105 3 0.16358890019401073 1105 4 0.10565469807260541 1105 5 -0.18853383430175796 1105 6 -0.05530043501948256 1105 7 -0.07313297599385038 1105 8 0.020546142486921326 1105 9 -0.037250787613736236 1105 10-11 0.055049252453414965 1105 12-13 0.13966407919549795 1105 14-15 0.08119139752258775 1105 16-17 0.08628554255804488 1105 18-19 -0.008431526207047568 1105 20-21 -0.08893031475243163 1105 22-23 -0.07958805076147968 1105 24-25 -0.11851276343442407 1105 26-27 -0.04084734362866982 1105 28-29 0.09742232087977243 1105 30-31 0.14061017637583006 1105 32-33 0.13118462130533004 1105 34-35 0.07764090477617458 1105 36-37 0.20659947547644464 1105 38-39 0.12865396276815488 1105 40-41 0.1724214542301823 1105 42-43 0.18346085695789327 1105 44-45 0.13150100260664743 1105 46-47 0.13149088478812132 1105 48-49 0.002833817130763805 1105 50-51 -0.22001864531537763 1105 52-53 -0.1531871246389258 1105 54-55 0.001758579032639318 1105 56-57 -0.040957182544147486 1105 58-59 -0.0809297965202731 1105 60-61 -0.10984647855263319 1105 62-63 -0.10913919679929762 1105 64-65 -0.24039789873587125 1105 66-67 -0.2513060980908506 1105 68-69 -0.18478848894596211 1105 70-71 -0.28861236768891985 1105 72-73 -0.4108395635074089 1105 74-75 -0.30145334828894477 1105 76-77 -0.33718814218728 1105 78-79 -0.3131630687004261 1105 80-81 -0.38917190045525274 1105 82-83 -0.3600170306342889 1105 84-85 -0.26020911128185986 1105 86-87 -0.3561077195688114 1105 88-89 -0.3838859603983735 1105 90-91 -0.35214168781709176 1105 92-93 -0.30728154409158037 1105 94-95 -0.27609181957800644 1105 96-97 -0.3552912526469747 1105 98-99 -0.37960678614168586 1105 100-101 -0.2370212431804397 1105 102-103 -0.16617629839915793 1105 104-105 -0.22980615339367816 1105 106-107 -0.26410734948383663 1105 108-109 -0.1894291845470093 1105 110-111 -0.17456690195979263 1105 112-113 -0.150378584539812 1105 114-115 -0.2053741100539206 1105 116-117 -0.1445350588804537 1105 118-119 -0.23646893836101057 1105 120-121 -0.21044497506768067 1105 122-123 -0.17184355397904483 1105 124-125 -0.16832021510240835 1106 1 -0.13837055424922795 1106 2 -0.03202035877929177 1106 3 -0.07004486375881669 1106 4 -0.16538278035718434 1106 5 -0.18144406743400765 1106 6 -0.014306291471918087 1106 7 -0.20836564767019894 1106 8 0.10409116921044159 1106 9 0.02420716404033385 1106 10-11 -0.18287194233657544 1106 12-13 -8.483041002094183E-4 1106 14-15 -0.11779288360350648 1106 16-17 -0.11593759626300937 1106 18-19 -0.10687969936644492 1106 20-21 -0.1511461662716087 1106 22-23 -0.011124770779659343 1106 24-25 -0.059641189848029796 1106 26-27 -0.02715704167644617 1106 28-29 -0.09984709026119276 1106 30-31 -0.00970628903711912 1106 32-33 -0.01574202053811291 1106 34-35 -0.039898054654429416 1106 36-37 -0.04684254646324604 1106 38-39 -1.035522619048379E-4 1106 40-41 0.055069794833755736 1106 42-43 0.03653882085299642 1106 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-0.7299760491234153 2216 80-81 -0.8290822528791892 2216 82-83 -0.8515506420029837 2216 84-85 -0.7454563305359656 2216 86-87 -0.7121164489691694 2216 88-89 -0.7208122182651877 2216 90-91 -0.6804421025165652 2216 92-93 -0.7887603995963524 2216 94-95 -0.7123944395749007 2216 96-97 -0.7589006914140803 2216 98-99 -0.7415047422993375 2216 100-101 -0.8159856595179988 2216 102-103 -0.7784467074029848 2216 104-105 -0.6470930844763672 2216 106-107 -0.7828529659550796 2216 108-109 -0.7371619881696283 2216 110-111 -0.7601177909041219 2216 112-113 -0.827236612340208 2216 114-115 -0.8061446696863896 2216 116-117 -0.7794577204885051 2216 118-119 -0.8185639003037402 2216 120-121 -0.8647981427408951 2216 122-123 -0.9849610725604983 2216 124-125 -1.0067056879115803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13175.0 3 2321.0 4 1596.0 5 1083.0 6 1168.0 7 1256.0 8 1360.0 9 1418.0 10 1725.0 11 1944.0 12 2222.0 13 2243.0 14 2466.0 15 2413.0 16 2273.0 17 2217.0 18 1948.0 19 1720.0 20 1566.0 21 1467.0 22 1533.0 23 2205.0 24 3548.0 25 6091.0 26 8080.0 27 6197.0 28 5965.0 29 7512.0 30 8203.0 31 9474.0 32 12366.0 33 17248.0 34 25967.0 35 46869.0 36 115920.0 37 276033.0 38 155160.0 39 696.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59843335960268 19.104603130107318 13.70387217229871 23.593091337991297 2 14.885383461155927 20.21896930536113 40.919475077574255 23.97617215590869 3 19.238588745389894 25.11308807783315 27.99323189995902 27.655091276817934 4 12.4998015337472 16.80453455694394 36.617949817146425 34.07771409216244 5 13.959378449295764 38.72953555542337 32.689839324780735 14.621246670500135 6 31.84961209153265 36.50874446694978 17.476109386485874 14.165534055031703 7 29.128898854537955 32.98716511364718 19.757369350693306 18.12656668112156 8 25.978384971879827 36.96701650960558 18.954228477912004 18.100370040602595 9 26.29700004234238 18.380165770419612 19.388311385866114 35.93452280137189 10-11 25.091728138292318 28.091221732858575 26.151543478807 20.66550665004211 12-13 25.162359874752372 25.046948030958934 26.93200814625179 22.85868394803691 14-15 22.424580481302332 26.484581684128578 24.600770734047845 26.490067100521248 16-17 22.6265232899096 29.82574541724064 25.260370533908322 22.28736075894144 18-19 22.643638871239563 28.457756112267774 26.931598270667394 21.967006745825266 20-21 23.62285323080529 27.614640570304516 26.60341867552642 22.159087523363773 22-23 23.331564818281773 27.484642365241136 26.367500955924285 22.81629186055281 24-25 22.885152320350866 27.67808843349368 26.539504887768476 22.897254358386977 26-27 22.633648578094125 27.971066214617572 26.440840388339247 22.954444818949053 28-29 22.59883757430372 27.89055091312206 26.67803319422554 22.83257831834868 30-31 22.874952252429672 27.638937168823514 26.575735907362446 22.910374671384368 32-33 22.5618600950428 27.73753595825571 26.525545313184594 23.175058633516894 34-35 23.117205136745493 27.81256112970335 26.31511469914934 22.75511903440181 36-37 23.169871818170403 27.50639190981476 26.2929037740849 23.03083249792994 38-39 22.769447939686803 27.665401330189376 26.533735844268445 23.03141488585537 40-41 22.919702433702152 27.549399983743157 26.567116637556342 22.963780944998344 42-43 22.801705052374185 27.42160393880316 26.507880910683014 23.268810098139642 44-45 22.78537563460499 27.68377935770904 26.522556316442696 23.00828869124328 46-47 22.929544810156365 27.838666121194056 26.277526153935632 22.954262914713947 48-49 22.945913703822423 27.62409578036344 26.38302231592583 23.046968199888305 50-51 22.94953692505548 27.67961584671935 26.44120263345374 22.92964459477142 52-53 23.116917620949433 27.55273807061175 26.312958044162578 23.01738626427624 54-55 22.854412384788134 27.498311393566244 26.577938274330144 23.06933794731548 56-57 22.821281683373464 27.854733826947697 26.371841906815142 22.9521425828637 58-59 22.915936894840154 27.40771828714566 26.51044592638613 23.16589889162806 60-61 22.881032219862195 27.50987003408747 26.717227323373642 22.891870422676693 62-63 22.622238965777054 27.818733394138874 26.605464567553632 22.953563072530436 64-65 22.659250299100385 27.489010952692567 26.5859591372689 23.26577961093815 66-67 22.688491866775067 27.787207603788676 26.391108643493087 23.13319188594317 68-69 22.765735090853926 27.499433175151193 26.619879654656593 23.11495207933829 70-71 22.614929974229035 27.44333395243575 26.754742247394645 23.186993825940565 72-73 22.660473575822625 27.500588202637793 26.668975417095155 23.16996280444443 74-75 22.607026803044512 27.77254586296525 26.454830291002406 23.165597042987834 76-77 22.790473591026878 27.60747346083412 26.479819131197953 23.12223381694105 78-79 22.87234077709752 27.481693521896062 26.61817105081398 23.027794650192433 80-81 22.680416459302823 27.843528364399905 26.515408372939003 22.96064680335827 82-83 22.86691901116383 27.723195667447232 26.46127490150435 22.948610419884588 84-85 22.851260834652383 27.651850358704237 26.422573748335353 23.074315058308027 86-87 22.69604191604497 27.565008007646096 26.701120806065585 23.03782927024335 88-89 22.786911034907607 27.670618438133715 26.57590547475458 22.9665650522041 90-91 22.698165935631927 27.850638895278284 26.57469905956942 22.87649610952037 92-93 22.682549408825935 27.501839259616208 26.66949578837641 23.146115543181452 94-95 22.599542606350635 27.529479417285778 26.726066150226053 23.144911826137534 96-97 22.710754180712538 27.56335514574658 26.70401216207284 23.021878511468042 98-99 22.487323502368724 27.492945382819983 26.644937101700844 23.37479401311045 100-101 22.351359621845155 27.491439369054564 26.80599406092803 23.351206948172244 102-103 22.630078167860624 27.32385131053118 26.78290274691354 23.263167774694658 104-105 22.391250034700054 27.466578892057058 26.85638672646973 23.28578434677316 106-107 22.465753062460177 27.602198784270072 26.71975019103194 23.21229796223781 108-109 22.582843304756686 27.49584106352449 26.501992389988942 23.41932324172988 110-111 22.299167968127804 27.797888097033596 26.711666489319867 23.191277445518732 112-113 22.37257910040128 27.763151893789423 26.804078737857083 23.060190267952215 114-115 22.20494369903735 27.730619819472107 26.803593639094903 23.260842842395636 116-117 22.171929553254387 27.761949795888423 26.837053735172418 23.229066915684772 118-119 22.187424398459612 27.77873255132973 26.625953653529127 23.40788939668153 120-121 21.997101052702543 27.913174347290713 26.885059317379206 23.20466528262753 122-123 22.123906672782383 27.844369158291705 26.76138534158338 23.27033882734253 124-125 22.23242958516763 27.84671419479217 26.635335202822873 23.285521017217327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 783.0 1 718.0 2 658.0 3 591.5 4 475.0 5 380.0 6 291.0 7 245.5 8 275.5 9 297.0 10 254.5 11 214.0 12 215.5 13 225.5 14 221.5 15 257.0 16 294.0 17 325.0 18 378.0 19 483.5 20 631.0 21 840.5 22 1119.0 23 1518.0 24 2038.5 25 2626.0 26 3496.0 27 4480.0 28 5461.5 29 6823.5 30 8496.0 31 10119.5 32 11741.5 33 13002.5 34 14178.5 35 15337.0 36 17141.5 37 19120.5 38 20158.0 39 20819.5 40 21228.5 41 22327.5 42 23670.5 43 24932.0 44 26424.0 45 27776.5 46 28172.0 47 28053.5 48 28577.0 49 28546.0 50 27957.0 51 27272.0 52 26720.0 53 26748.0 54 26410.0 55 25085.5 56 22687.0 57 20606.0 58 19150.5 59 16997.0 60 14786.5 61 12395.5 62 10067.5 63 7857.0 64 6034.5 65 4855.0 66 3610.0 67 2877.5 68 2227.5 69 1494.0 70 1089.5 71 828.5 72 659.5 73 579.5 74 462.0 75 341.0 76 237.5 77 147.0 78 96.0 79 75.0 80 57.5 81 34.0 82 25.5 83 25.0 84 18.0 85 16.0 86 12.0 87 6.5 88 7.5 89 6.0 90 4.0 91 5.5 92 6.5 93 3.5 94 1.0 95 2.5 96 4.0 97 3.0 98 3.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05022150326175447 2 0.20683329632801514 3 0.020881572408834755 4 0.11260189678688108 5 0.02022076315539062 6 0.03753396559562703 7 0.0359480233873611 8 0.10374705279072964 9 0.11947431302270012 10-11 0.04453854368213489 12-13 0.02993465918101944 14-15 0.01275361859147186 16-17 0.02986857825567503 18-19 0.0531951449022531 20-21 0.1946083251392986 22-23 0.11035514532517102 24-25 0.0762573878474535 26-27 0.012291052114060964 28-29 0.005352554952897517 30-31 0.008590520294773791 32-33 0.005484716803586344 34-35 0.007929711041329654 36-37 0.003634450893942758 38-39 0.008458358444084964 40-41 0.005749040504963999 42-43 0.009647815100284412 44-45 0.009912138801662067 46-47 0.01552901745593724 48-49 0.01658631226144786 50-51 0.009912138801662067 52-53 0.013546589695604825 54-55 0.014141318023704549 56-57 0.015793341157314893 58-59 0.017577526141614067 60-61 0.008590520294773791 62-63 0.018238335395058206 64-65 0.02815047419672027 66-67 0.024185618676055443 68-69 0.031917086941351855 70-71 0.023326566646578065 72-73 0.014009156173015722 74-75 0.009713896025628827 76-77 0.010242543428384135 78-79 0.02028684408073503 80-81 0.016850635962825513 82-83 0.018833063723157926 84-85 0.013943075247671308 86-87 0.02425169960139986 88-89 0.02563939903363255 90-91 0.024582104228121928 92-93 0.030331144733085926 94-95 0.024846427929499582 96-97 0.02616804643638786 98-99 0.0301989828823971 100-101 0.017511445216269652 102-103 0.026300208287076687 104-105 0.02180670536365655 106-107 0.03039722565843034 108-109 0.019097387424535584 110-111 0.016718474112136686 112-113 0.024912508854843994 114-115 0.011762404711305654 116-117 0.01011038157769531 118-119 0.028414797898097928 120-121 0.02200494813968979 122-123 0.0167845550374811 124-125 0.01500037005318193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 756648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.76702145454144 #Duplication Level Percentage of deduplicated Percentage of total 1 81.30338967749766 52.65778383569434 2 11.086262100060999 14.360483505706407 3 3.3182506346184035 6.447396301316275 4 1.4119763503776857 3.657980103128667 5 0.7404325439287752 2.3977805229137843 6 0.4496226631460626 1.7472432402257456 7 0.314087139221567 1.4239741939191142 8 0.2172940434278573 1.1258790378108874 9 0.16656812767513665 0.9709309348900534 >10 0.9204686171319124 10.654756359906632 >50 0.05342950016522804 2.321548816489076 >100 0.01801389934735557 2.0995415673946574 >500 0.0 0.0 >1k 2.0470340134986234E-4 0.13470158060436846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1016 0.1342764402998488 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.608092534441378E-5 0.0 24-25 0.0 0.0 0.0 1.3216185068882756E-4 0.0 26-27 0.0 0.0 0.0 3.964855520664827E-4 0.0 28-29 0.0 0.0 0.0 9.25132954821793E-4 0.0 30-31 0.0 0.0 0.0 0.003105803491187448 0.0 32-33 0.0 0.0 0.0 0.0063437688330637235 0.0 34-35 0.0 0.0 0.0 0.009846057876317654 1.3216185068882756E-4 36-37 0.0 0.0 0.0 0.020352925006079443 1.3216185068882756E-4 38-39 0.0 0.0 0.0 0.037600046520971445 1.3216185068882756E-4 40-41 0.0 0.0 0.0 0.057490405049639995 1.3216185068882756E-4 42-43 0.0 0.0 0.0 0.07401063638574343 1.3216185068882756E-4 44-45 0.0 0.0 0.0 0.09515653249595585 1.3216185068882756E-4 46-47 0.0 0.0 0.0 0.12258011651388756 1.3216185068882756E-4 48-49 0.0 0.0 0.0 0.15304342309766233 1.3216185068882756E-4 50-51 0.0 0.0 0.0 0.18952009388777874 1.3216185068882756E-4 52-53 0.0 0.0 0.0 0.22573244097651748 2.6432370137765513E-4 54-55 0.0 0.0 0.0 0.263662892124211 2.6432370137765513E-4 56-57 0.0 0.0 0.0 0.30839967858237916 2.6432370137765513E-4 58-59 0.0 0.0 0.0 0.361396580708599 2.6432370137765513E-4 60-61 0.0 0.0 0.0 0.4243717025618253 2.6432370137765513E-4 62-63 0.0 0.0 0.0 0.48397669722248654 3.964855520664827E-4 64-65 0.0 0.0 0.0 0.5428548017043593 3.964855520664827E-4 66-67 0.0 0.0 0.0 0.6129005825694378 3.964855520664827E-4 68-69 0.0 0.0 0.0 0.6958982248020216 3.964855520664827E-4 70-71 0.0 0.0 0.0 0.783323289032681 3.964855520664827E-4 72-73 0.0 0.0 0.0 0.8795371163341474 4.625664774108965E-4 74-75 0.0 0.0 0.0 0.9802444465590341 5.94728328099724E-4 76-77 0.0 0.0 0.0 1.085313117856652 6.608092534441378E-4 78-79 0.0 0.0 0.0 1.2209111766633889 7.929711041329654E-4 80-81 0.0 0.0 0.0 1.3656944840929996 7.929711041329654E-4 82-83 0.0 0.0 0.0 1.518737907190662 0.0010572948055106205 84-85 0.0 0.0 0.0 1.6655697233059494 0.0010572948055106205 86-87 0.0 0.0 0.0 1.8234370539537539 0.0010572948055106205 88-89 0.0 0.0 0.0 2.015269980228587 0.0010572948055106205 90-91 0.0 0.0 0.0 2.236905403833751 0.0010572948055106205 92-93 0.0 0.0 0.0 2.4672635095843773 0.001189456656199448 94-95 0.0 0.0 0.0 2.6919386557553846 0.001189456656199448 96-97 0.0 0.0 0.0 2.9259312123999535 0.001189456656199448 98-99 0.0 0.0 0.0 3.1806070986773243 0.001189456656199448 100-101 0.0 0.0 0.0 3.488412048931604 0.001189456656199448 102-103 0.0 0.0 0.0 3.80949926518011 0.001189456656199448 104-105 0.0 0.0 0.0 4.128405810892251 0.001189456656199448 106-107 0.0 0.0 0.0 4.449162622514036 0.001189456656199448 108-109 0.0 0.0 0.0 4.7925851915289535 0.001189456656199448 110-111 0.0 0.0 0.0 5.1886742580433705 0.001189456656199448 112-113 0.0 0.0 0.0 5.6115921802476185 0.001189456656199448 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1455 0.0 53.71332 1 GGTATCA 910 0.0 51.136017 1 ATCAACG 1800 0.0 41.70026 3 TCAACGC 1820 0.0 41.57759 4 CAACGCA 1830 0.0 41.333965 5 AACGCAG 1970 0.0 38.396526 6 TATCAAC 2110 0.0 36.191055 2 GTACATG 4115 0.0 34.214947 1 TACATGG 4065 0.0 34.195507 2 ACGCAGA 2320 0.0 32.603947 7 GTATAGG 300 0.0 31.817966 1 ACATGGG 4280 0.0 31.73451 3 CGCAGAG 2455 0.0 30.568455 8 GTGTACG 80 0.0052916966 29.829348 1 TACGTTA 40 0.005365806 29.746513 102-103 GCAGAGT 2680 0.0 27.324505 9 CATGGGG 1955 0.0 26.820253 4 CATGGGA 2545 0.0 26.689722 4 TATAGGG 145 1.3154797E-4 24.686354 2 TAGGGTG 290 1.4370016E-10 24.645504 5 >>END_MODULE