FastQCFastQC Report
Fri 27 May 2016
SRR940073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756648
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.5990896691724553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.46098053520263055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.3006682103170827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.12674321481058565No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.075.3292851
GTATCAA36950.061.1409451
TATAACG1103.6379788E-1254.1858562
TAACGCA1350.048.566584
ATCAACG47150.046.8997883
TCAACGC47350.046.7016874
CAACGCA47900.045.9862065
TATCAAC48800.045.6804542
AACGCAG49650.044.344826
ATAACGC1404.0017767E-1142.5746043
GTGGTAT6900.038.8750341
ACGCAGA57150.038.4135447
CGCAGAG57850.037.8458948
GCAGAGT63600.034.23729
TGGTATC7850.033.408862
GTGTAGC3900.032.0968251
GAGTACT41250.030.7927312-13
GTACATG40700.030.4632551
TACATGG41450.030.0538672
GTATAGG2601.8189894E-1229.8041931
Produced by FastQC (version 0.11.5)