##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 756648 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.12814280880938 34.0 33.0 34.0 31.0 34.0 2 33.16992445628615 34.0 33.0 34.0 31.0 34.0 3 33.22728534272211 34.0 33.0 34.0 31.0 34.0 4 36.45717823875831 37.0 37.0 37.0 35.0 37.0 5 36.46866706843869 37.0 37.0 37.0 35.0 37.0 6 36.55001665239319 37.0 37.0 37.0 35.0 37.0 7 36.542942028525815 37.0 37.0 37.0 35.0 37.0 8 36.53807186432793 37.0 37.0 37.0 35.0 37.0 9 38.40673999006143 39.0 39.0 39.0 37.0 39.0 10-11 38.3862443302566 39.0 39.0 39.0 37.0 39.0 12-13 38.36888090631311 39.0 39.0 39.0 37.0 39.0 14-15 39.97106514521944 41.0 40.0 41.0 38.0 41.0 16-17 39.98320288958671 41.0 40.0 41.0 38.0 41.0 18-19 40.03252238821751 41.0 40.0 41.0 38.0 41.0 20-21 40.03470041551686 41.0 40.0 41.0 38.0 41.0 22-23 39.99727284021104 41.0 40.0 41.0 38.0 41.0 24-25 39.966438158826826 41.0 40.0 41.0 38.0 41.0 26-27 39.87977699009315 41.0 40.0 41.0 38.0 41.0 28-29 39.7915100548736 41.0 40.0 41.0 37.0 41.0 30-31 39.63838403061926 41.0 40.0 41.0 36.5 41.0 32-33 39.45459183134033 41.0 40.0 41.0 35.0 41.0 34-35 39.34527019168755 41.0 40.0 41.0 35.0 41.0 36-37 39.24257316480055 41.0 39.0 41.0 35.0 41.0 38-39 39.12503700531819 41.0 39.0 41.0 35.0 41.0 40-41 38.98598621816221 41.0 39.0 41.0 35.0 41.0 42-43 38.81204139837811 41.0 39.0 41.0 35.0 41.0 44-45 38.59177979721086 41.0 39.0 41.0 35.0 41.0 46-47 38.2632102377856 41.0 38.5 41.0 34.5 41.0 48-49 37.96405263742189 41.0 38.0 41.0 34.0 41.0 50-51 37.96286978885823 41.0 38.0 41.0 34.0 41.0 52-53 37.85427371776572 41.0 38.0 41.0 34.0 41.0 54-55 37.68697267421575 41.0 37.5 41.0 34.0 41.0 56-57 37.50058283376154 40.5 37.0 41.0 34.0 41.0 58-59 37.29183781626331 40.0 36.5 41.0 33.0 41.0 60-61 37.056817040420384 40.0 36.0 41.0 33.0 41.0 62-63 36.800557194362504 39.0 35.0 41.0 33.0 41.0 64-65 36.49550451464882 39.0 35.0 41.0 33.0 41.0 66-67 36.17437434579884 38.5 35.0 41.0 32.0 41.0 68-69 35.82973522694833 37.0 35.0 40.5 32.0 41.0 70-71 35.47279514384496 37.0 35.0 39.5 32.0 41.0 72-73 35.110717929605315 36.5 35.0 39.0 31.0 41.0 74-75 34.75776583034647 36.0 35.0 39.0 31.0 41.0 76-77 34.402190318351465 35.5 35.0 37.5 31.0 39.5 78-79 34.07274915152092 35.0 35.0 37.0 31.0 39.0 80-81 33.76701055180216 35.0 35.0 37.0 31.0 39.0 82-83 33.49480339603092 35.0 35.0 36.0 30.0 38.0 84-85 33.25864338503505 35.0 35.0 36.0 30.0 37.0 86-87 33.05774019094744 35.0 35.0 35.5 30.0 37.0 88-89 32.949956915236676 35.0 35.0 35.0 30.0 36.5 90-91 32.949003367483954 35.0 35.0 35.0 31.0 36.0 92-93 32.86475217009759 35.0 35.0 35.0 31.0 36.0 94-95 32.788214599126675 35.0 35.0 35.0 30.5 36.0 96-97 32.721863402797595 35.0 35.0 35.0 30.0 36.0 98-99 32.668698655121005 35.0 35.0 35.0 30.0 35.5 100-101 32.61529932015944 35.0 35.0 35.0 30.0 35.0 102-103 32.13904285744494 35.0 33.5 35.0 28.5 35.0 104-105 32.51721011619669 35.0 34.5 35.0 30.0 35.0 106-107 32.559828348188326 35.0 35.0 35.0 30.0 35.0 108-109 32.5479469978114 35.0 35.0 35.0 30.0 35.0 110-111 32.51110159545786 35.0 35.0 35.0 30.0 35.0 112-113 32.48163478922828 35.0 35.0 35.0 30.0 35.0 114-115 32.42629928315412 35.0 35.0 35.0 30.0 35.0 116-117 32.371529429800916 35.0 35.0 35.0 30.0 35.0 118-119 32.312362551675285 35.0 35.0 35.0 29.0 35.0 120-121 32.23647125215424 35.0 35.0 35.0 29.0 35.0 122-123 32.13812829743817 35.0 35.0 35.0 29.0 35.0 124-125 31.09408469988687 34.5 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09309676147498891 1101 2 0.06448657188592932 1101 3 0.1311962483693918 1101 4 0.05014128125557704 1101 5 0.007732947676238666 1101 6 0.021905189754754417 1101 7 0.024607960310873978 1101 8 0.09737970643721638 1101 9 0.14156703966845186 1101 10-11 0.1180003507931886 1101 12-13 0.10322974403517549 1101 14-15 0.20524870468514678 1101 16-17 0.19419975446611915 1101 18-19 0.15459156050435752 1101 20-21 0.14788324847216217 1101 22-23 0.20580599774751818 1101 24-25 0.14053796709069388 1101 26-27 0.10194209514381214 1101 28-29 0.16426596913562008 1101 30-31 0.08288954010143357 1101 32-33 0.16834440191257016 1101 34-35 0.1452970655082737 1101 36-37 0.27753828233658595 1101 38-39 0.3402327053603429 1101 40-41 0.2847367768220366 1101 42-43 0.35924497095236063 1101 44-45 0.37463063624037574 1101 46-47 0.43568019859059604 1101 48-49 0.49390360728831695 1101 50-51 0.4877823593184232 1101 52-53 0.370620562121168 1101 54-55 0.3939820819881561 1101 56-57 0.36358680821848566 1101 58-59 0.4208910838320108 1101 60-61 0.310874188276685 1101 62-63 0.3486918845104583 1101 64-65 0.3055567980519456 1101 66-67 0.31554727084974843 1101 68-69 0.3353189162140211 1101 70-71 0.3513615833797843 1101 72-73 0.4495343129772138 1101 74-75 0.39520472202604395 1101 76-77 0.4672184964675097 1101 78-79 0.45541231421639594 1101 80-81 0.3475137688315044 1101 82-83 0.35688472390116743 1101 84-85 0.37237575346337337 1101 86-87 0.40380825862236946 1101 88-89 0.2208949600764143 1101 90-91 0.2973093858910829 1101 92-93 0.3822914694695214 1101 94-95 0.3169458288704945 1101 96-97 0.254325064666709 1101 98-99 0.24325605142600892 1101 100-101 0.2594904274399923 1101 102-103 0.1637778878967424 1101 104-105 0.3206430296066287 1101 106-107 0.2167901542834585 1101 108-109 0.23598601089298654 1101 110-111 0.27065823152316426 1101 112-113 0.21998993982173687 1101 114-115 0.19988581074230183 1101 116-117 0.29303197816290805 1101 118-119 0.2815941463868725 1101 120-121 0.28047958671706397 1101 122-123 0.33347014773128336 1101 124-125 0.23789067150814702 1102 1 0.2027818278973399 1102 2 0.16742404325560756 1102 3 0.1650246954605521 1102 4 -0.003691746687621844 1102 5 0.11537380887642001 1102 6 0.16890129603052628 1102 7 0.21298290305022505 1102 8 0.2039188727221415 1102 9 0.11859979276052712 1102 10-11 0.2515150095197143 1102 12-13 0.23145122823123643 1102 14-15 0.32503340463934194 1102 16-17 0.368441623453748 1102 18-19 0.3423108687956997 1102 20-21 0.3268050304235999 1102 22-23 0.31363352866826943 1102 24-25 0.2666127495002186 1102 26-27 0.2828032953270423 1102 28-29 0.3297813149899511 1102 30-31 0.2689402730377566 1102 32-33 0.320271222801253 1102 34-35 0.2878703591408893 1102 36-37 0.2246414660287499 1102 38-39 0.2661889582233812 1102 40-41 0.11261011626316986 1102 42-43 0.18208567182272617 1102 44-45 0.25481100728938344 1102 46-47 0.22982873271794801 1102 48-49 0.2048501258407711 1102 50-51 0.18441715088965793 1102 52-53 0.2520182945994094 1102 54-55 0.21209877919383757 1102 56-57 0.2616445269541714 1102 58-59 0.3295735162644391 1102 60-61 0.2944466802601937 1102 62-63 0.3541167585370246 1102 64-65 0.3825632112448005 1102 66-67 0.3295652506939959 1102 68-69 0.2608703362781526 1102 70-71 0.36834967316448086 1102 72-73 0.3658014911723555 1102 74-75 0.32681088327203867 1102 76-77 0.3367289179059014 1102 78-79 0.38980019786275477 1102 80-81 0.42310412613795734 1102 82-83 0.3732418013175689 1102 84-85 0.34710204755407403 1102 86-87 0.4168430730978585 1102 88-89 0.5047440210017484 1102 90-91 0.48046902858462914 1102 92-93 0.4373384232029167 1102 94-95 0.4115747798553784 1102 96-97 0.4320300577954299 1102 98-99 0.45759068266741565 1102 100-101 0.4677663092540101 1102 102-103 0.5472621984785135 1102 104-105 0.4329701024421766 1102 106-107 0.474889673293994 1102 108-109 0.4222440273840533 1102 110-111 0.3945359227737413 1102 112-113 0.421143169322427 1102 114-115 0.44603789502997415 1102 116-117 0.4236458123360691 1102 118-119 0.5501151037849397 1102 120-121 0.548727983418857 1102 122-123 0.5962982512585455 1102 124-125 0.6983756692177181 1103 1 0.1132191480124689 1103 2 0.11651218240211136 1103 3 0.10521630616303668 1103 4 -0.029508557262182933 1103 5 0.06189692297228788 1103 6 0.04061673148516576 1103 7 0.06752710587152677 1103 8 0.049195673347519175 1103 9 0.1391533051792493 1103 10-11 0.08895904533678589 1103 12-13 0.08390343659566213 1103 14-15 0.1701458659974051 1103 16-17 0.14899096076675988 1103 18-19 0.18545903174918266 1103 20-21 0.12141092709417478 1103 22-23 0.10525355851582674 1103 24-25 0.1874001122547213 1103 26-27 0.1811794457019147 1103 28-29 0.13373789389056157 1103 30-31 0.1972183972604853 1103 32-33 0.16446316218227253 1103 34-35 0.15040500363508613 1103 36-37 0.15654615813691208 1103 38-39 0.2329546726106173 1103 40-41 0.2803982873892039 1103 42-43 0.2893225957469667 1103 44-45 0.23951079942242615 1103 46-47 0.2568547693878074 1103 48-49 0.16864551901136338 1103 50-51 0.05506549150722151 1103 52-53 -0.03354227996619841 1103 54-55 -0.07966005071057936 1103 56-57 -0.037150910632441025 1103 58-59 8.91650436351199E-4 1103 60-61 0.07559230261743721 1103 62-63 -0.09015224263556121 1103 64-65 -0.02685920285462373 1103 66-67 -0.02240615427349013 1103 68-69 0.004848634611661851 1103 70-71 -0.02508297438553342 1103 72-73 -0.04797036150859668 1103 74-75 0.06403128443751172 1103 76-77 -0.004191781735215727 1103 78-79 -0.03850235516996747 1103 80-81 -0.05950589233910364 1103 82-83 0.009885460486806608 1103 84-85 -3.9777478185953896E-4 1103 86-87 0.05810821896006502 1103 88-89 -0.06283272987768385 1103 90-91 -0.024538877466611098 1103 92-93 -0.06000327810824757 1103 94-95 -0.05577500517109257 1103 96-97 -0.08254727937545425 1103 98-99 -0.06463731363717073 1103 100-101 -0.08331766366998039 1103 102-103 -0.07878712995128723 1103 104-105 -0.040602366733104134 1103 106-107 -0.004839966403267226 1103 108-109 -0.053239440973968044 1103 110-111 -0.05979108571860081 1103 112-113 -0.003320729337985995 1103 114-115 -0.00533544825254495 1103 116-117 -0.07427551801250587 1103 118-119 -0.11184457535374293 1103 120-121 -0.03130635016320582 1103 122-123 -0.06652531943398543 1103 124-125 0.0034615253808851776 1104 1 0.13160278289682026 1104 2 0.0342518196799233 1104 3 0.03445836058427432 1104 4 -0.01897687180508001 1104 5 0.015732281712530494 1104 6 0.11638546057999832 1104 7 0.10487767561139094 1104 8 0.10965008770143214 1104 9 0.10507222863565602 1104 10-11 0.11626329546270142 1104 12-13 0.12695248114600588 1104 14-15 0.20204763914321688 1104 16-17 0.2112878281660997 1104 18-19 0.16632528601632401 1104 20-21 0.23713570421332264 1104 22-23 0.11354782581788925 1104 24-25 0.10931647866181038 1104 26-27 0.10226175772220358 1104 28-29 0.10742374703672652 1104 30-31 0.060246219162422676 1104 32-33 0.02825616172166434 1104 34-35 0.1308623022028783 1104 36-37 0.020956896022134686 1104 38-39 -0.09173355247860826 1104 40-41 -0.03553182853897141 1104 42-43 -0.031043613319795327 1104 44-45 -0.09302184169958139 1104 46-47 -0.14558346753960194 1104 48-49 -0.146399961167198 1104 50-51 -0.11969419320946173 1104 52-53 -0.12557345530488107 1104 54-55 -0.14469154029943354 1104 56-57 -0.16767019827466356 1104 58-59 -0.1274978389716992 1104 60-61 -0.22491803104736618 1104 62-63 -0.12637387940541345 1104 64-65 -0.1254110691992878 1104 66-67 -0.03731581811190665 1104 68-69 -0.14440082406013488 1104 70-71 -0.08853352733667919 1104 72-73 -0.116452145760789 1104 74-75 -0.15478584348813484 1104 76-77 -0.11729063278494323 1104 78-79 -0.12077670848186983 1104 80-81 -0.16234748872996363 1104 82-83 -0.0342796026430392 1104 84-85 -0.0795350074001675 1104 86-87 -0.02175975292099963 1104 88-89 0.06695689470097932 1104 90-91 0.05507618760039179 1104 92-93 0.031391863498043904 1104 94-95 -0.044333931382574576 1104 96-97 -0.05279575224877675 1104 98-99 -0.07486981571423001 1104 100-101 -0.009340806257760903 1104 102-103 0.015688093790522828 1104 104-105 0.16411547535833648 1104 106-107 0.13660812420409485 1104 108-109 0.12210677231148992 1104 110-111 0.049275246895831515 1104 112-113 0.08252337674942822 1104 114-115 -0.019907185539402406 1104 116-117 5.956609421957637E-4 1104 118-119 -0.0053557398443331294 1104 120-121 -0.03449405221961399 1104 122-123 0.010568210886845009 1104 124-125 -0.05813796493116996 1105 1 0.1506369983410636 1105 2 0.07703108780123102 1105 3 0.144391179766572 1105 4 -8.340658995464878E-4 1105 5 0.06117633999603811 1105 6 0.0740676279846113 1105 7 0.037112722478553906 1105 8 0.07151768506957268 1105 9 0.01862857515695282 1105 10-11 0.06865049020736791 1105 12-13 0.17025401038529253 1105 14-15 0.15657631421500184 1105 16-17 0.19122429688391662 1105 18-19 0.18400822947159412 1105 20-21 0.15743739914991295 1105 22-23 0.08122216979290187 1105 24-25 0.1741806802839676 1105 26-27 0.09044166723889191 1105 28-29 0.14863832923831666 1105 30-31 0.18928823706193043 1105 32-33 0.13028847059820947 1105 34-35 0.20437003019921463 1105 36-37 0.14969740775413243 1105 38-39 0.14339091975450913 1105 40-41 0.0947293229943611 1105 42-43 0.06902549713738182 1105 44-45 0.12550825543259947 1105 46-47 0.16929751355622358 1105 48-49 0.11277945964732083 1105 50-51 0.022869458675188525 1105 52-53 -0.04112446175868456 1105 54-55 -0.017403501756980688 1105 56-57 0.011207380782877863 1105 58-59 -0.07437822020134632 1105 60-61 -0.009695063303801987 1105 62-63 -0.07991204117212192 1105 64-65 0.07223487551654273 1105 66-67 0.07013468768435871 1105 68-69 0.1312927449968413 1105 70-71 0.09570247176561253 1105 72-73 0.03553160751383189 1105 74-75 0.08965962636858649 1105 76-77 0.13133135293992382 1105 78-79 0.05082959053254399 1105 80-81 0.15657210193478477 1105 82-83 0.19653411793257902 1105 84-85 0.2231981591172385 1105 86-87 0.23112736526731936 1105 88-89 0.2648167777061019 1105 90-91 0.30557485258351846 1105 92-93 0.29267658389957774 1105 94-95 0.2530140728039072 1105 96-97 0.2571845738183569 1105 98-99 0.25537487096055855 1105 100-101 0.2350600931220228 1105 102-103 0.2000929363466284 1105 104-105 0.19113304975957135 1105 106-107 0.24678214832039913 1105 108-109 0.20172380179931793 1105 110-111 0.14887925131721857 1105 112-113 0.1330584322160746 1105 114-115 0.06413228397754267 1105 116-117 0.14396315448734498 1105 118-119 0.1425222859666917 1105 120-121 0.2282635913550024 1105 122-123 0.1786088717464125 1105 124-125 0.3121976035678884 1106 1 0.10098713682027238 1106 2 0.1290697923809745 1106 3 0.08395365507469421 1106 4 0.05242260065112703 1106 5 0.030712961758460722 1106 6 0.008710151028658686 1106 7 0.13389464178192156 1106 8 0.07959819747333796 1106 9 0.09142892560248583 1106 10-11 0.15580027496586268 1106 12-13 0.10338789818131033 1106 14-15 0.14104678733693987 1106 16-17 0.1837502067219816 1106 18-19 0.14507848028987524 1106 20-21 0.12860035836762762 1106 22-23 0.19711022782929888 1106 24-25 0.10241252702135739 1106 26-27 0.16203633768294168 1106 28-29 0.12513473369175898 1106 30-31 -0.007495012788126587 1106 32-33 0.034238351974856585 1106 34-35 -0.07021233166445029 1106 36-37 -0.10231222949789753 1106 38-39 -0.1253037730017894 1106 40-41 0.0026677739514298082 1106 42-43 0.0066765909491124376 1106 44-45 -0.07476870297860927 1106 46-47 -0.1976989922234651 1106 48-49 -0.06470062820970668 1106 50-51 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-0.5208968915589054 2216 62-63 -0.4910123127743873 2216 64-65 -0.45087426434686506 2216 66-67 -0.3557648477204367 2216 68-69 -0.47333502574637976 2216 70-71 -0.44052812891315085 2216 72-73 -0.43078671081155306 2216 74-75 -0.32174072572586 2216 76-77 -0.4463224177185623 2216 78-79 -0.4307472324270236 2216 80-81 -0.4547003923342743 2216 82-83 -0.4591558789136272 2216 84-85 -0.3654409111274859 2216 86-87 -0.3435011821548102 2216 88-89 -0.4166171834034742 2216 90-91 -0.296536360777246 2216 92-93 -0.29846080354082005 2216 94-95 -0.3561407946193569 2216 96-97 -0.36463869171640084 2216 98-99 -0.3987320541911359 2216 100-101 -0.3733866431790105 2216 102-103 -0.5326824434022939 2216 104-105 -0.47700705134990784 2216 106-107 -0.44702071880161043 2216 108-109 -0.48202401526219774 2216 110-111 -0.4416938671192554 2216 112-113 -0.3265722098855761 2216 114-115 -0.37598135827003176 2216 116-117 -0.46088320430066076 2216 118-119 -0.4579811431884231 2216 120-121 -0.5232985923836893 2216 122-123 -0.5583261531779158 2216 124-125 -0.7941871090373773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 14.0 5 34.0 6 92.0 7 227.0 8 338.0 9 553.0 10 965.0 11 1646.0 12 2005.0 13 3513.0 14 6385.0 15 6728.0 16 2970.0 17 2271.0 18 2219.0 19 1802.0 20 1551.0 21 1381.0 22 1397.0 23 1544.0 24 1877.0 25 2307.0 26 2860.0 27 3636.0 28 4443.0 29 5284.0 30 6575.0 31 8340.0 32 10546.0 33 14038.0 34 20773.0 35 38759.0 36 95534.0 37 295444.0 38 206692.0 39 1894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60431749774951 18.66390483580369 14.080182101062388 22.651595565384408 2 15.5725430955298 19.687483892455127 41.1306406144062 23.60933239760888 3 19.302924613459115 25.03703840760619 29.01564401894146 26.644392959993233 4 12.513152548384834 16.48677730469045 37.092502617274405 33.907567529650315 5 13.540599321747063 37.65480495270078 33.27723092992048 15.527364795631682 6 31.431736141493634 36.506039862165046 17.133143966680635 14.929080029660685 7 28.508500650236307 33.20962984108859 20.044194922870346 18.23767458580476 8 26.441885791015107 35.37417663167021 18.999984140577915 19.183953436736765 9 26.959035112760493 16.448203127478035 19.803660354616678 36.7891014051448 10-11 25.44135450037534 26.923549655850543 26.136988401475985 21.498107442298135 12-13 26.25619839079731 24.152379970607203 27.42794535900445 22.163476279591038 14-15 22.96325371903448 25.054384601558453 26.23439168543365 25.74796999397342 16-17 21.96080608155972 28.73125944957232 26.67257430139246 22.635360167475497 18-19 21.76044871591546 26.328953489601503 30.256341125596048 21.654256668886987 20-21 22.75833676954145 25.435539378945034 30.40026538099618 21.405858470517334 22-23 22.371366870724565 25.339788118120975 30.326320825535785 21.962524185618676 24-25 21.987965341876276 25.653011704253498 30.41116873367801 21.947854220192216 26-27 21.777629756505007 25.93484685084742 30.308214651991417 21.979308740656155 28-29 21.681789316411297 25.82368384708051 30.686390540364624 21.808136296143577 30-31 21.9469435899808 25.57148474025223 30.44171823735623 22.039853432410734 32-33 21.74919199388354 25.718152400097004 30.338539859762783 22.194115746256674 34-35 22.24717155810952 25.846021480364957 30.039800725439754 21.86700623608577 36-37 22.30894379860329 25.565595610042347 30.06518325852854 22.060277332825823 38-39 21.99743863901595 25.725950234527094 30.133129975192595 22.143481151264357 40-41 22.18838878844588 25.590829553504403 30.03939744769034 22.181384210359376 42-43 22.19095090858989 25.518889775117476 29.83699635030949 22.45316296598314 44-45 22.221935869275033 25.966639441109535 29.560611226973922 22.250813462641513 46-47 22.461347538117206 26.17807655226053 28.955727674680315 22.404848234941944 48-49 22.663262986498058 26.228007803000665 28.637237124520244 22.471492085981033 50-51 22.71834869842243 26.49769143693926 28.289297611345958 22.494662253292354 52-53 22.836078118329947 26.48011556247497 28.16835327438026 22.51545304481482 54-55 22.51522504519935 26.534134762795908 28.259705966314584 22.69093422569015 56-57 22.579696564594183 26.815818184581612 28.125828090275967 22.478657160548245 58-59 22.66164706006743 26.31559818300286 28.3400935460124 22.682661210917313 60-61 22.539906798231122 26.400471559715477 28.679170224070162 22.380451417983235 62-63 22.38522216120738 26.494142244760265 28.591885378442903 22.528750215589447 64-65 22.34107440108216 26.286699474185394 28.61556191851955 22.7566642062129 66-67 22.36245458546391 26.568412232485418 28.326709760639456 22.74242342141121 68-69 22.412119409889147 26.27486742330376 28.663593860996844 22.649419305810245 70-71 22.35352736360819 26.3106559375762 28.515551432032243 22.82026526678337 72-73 22.494079149089142 26.51873790719066 28.173404277814782 22.813778665905417 74-75 22.510115357700062 26.739633369083183 28.02768531936644 22.722565953850314 76-77 22.627777744735937 26.58493437560508 28.03117563521282 22.756112244446165 78-79 22.590156632962902 26.605334230279464 28.01326652053846 22.791242616219172 80-81 22.455487888688 26.919716962180562 27.92916917774183 22.695625971389603 82-83 22.73553885029763 26.7714313657077 27.810818240449986 22.682211543544685 84-85 22.683145541736522 26.686737712706943 27.70835219399309 22.92176455156345 86-87 22.51521847733286 26.594074375599686 28.031734787469965 22.858972359597487 88-89 22.603112675907422 26.71030650976412 27.826281176980576 22.86029963734788 90-91 22.490951851359913 26.875760345154575 27.868562135686876 22.764725667798636 92-93 22.46651815952469 26.49867870054709 27.970908283513396 23.063894856414827 94-95 22.401582790196375 26.619977704015856 27.935401119557095 23.043038386230677 96-97 22.56247493039985 26.476867904105433 27.905810332078453 23.054846833416264 98-99 22.427799980968693 26.440630253433568 27.7183049449678 23.413264820629937 100-101 22.33286415690317 26.47159500161899 27.903574331423584 23.29196651005425 102-103 22.571525993592793 26.10745022784703 27.936900646007125 23.38412313255305 104-105 22.381888528013373 26.18901139566311 28.04634919166455 23.38275088465897 106-107 22.499983479703165 26.25147856656688 27.823748257108683 23.424789696621268 108-109 22.557563550529842 26.173143970332074 27.627398426204202 23.641894052933885 110-111 22.357291633626204 26.31434960510039 27.847691396792168 23.48066736448124 112-113 22.399627831490662 26.344138246856357 27.801159458214634 23.455074463438343 114-115 22.184643383205696 26.368648410752133 27.77838353280296 23.66832467323922 116-117 22.174548285225097 26.325771217526857 27.843789256381303 23.655891240866747 118-119 22.12551601181268 26.406059913208914 27.62420132572166 23.84422274925675 120-121 22.069420792933027 26.48641076116786 27.780343925465857 23.663824520433256 122-123 22.080676038319236 26.48486374362563 27.559676386567784 23.87478383148735 124-125 22.137821247767267 26.418591680714528 27.534380639657325 23.90920643186088 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 166.0 1 135.5 2 263.0 3 394.0 4 220.0 5 126.5 6 407.5 7 1143.0 8 2047.5 9 2227.0 10 1518.0 11 875.0 12 741.5 13 781.5 14 868.0 15 941.0 16 1028.0 17 1157.5 18 1246.0 19 1316.5 20 1429.5 21 1594.0 22 1807.5 23 2187.0 24 2647.5 25 3162.0 26 3940.0 27 4870.0 28 5880.5 29 7268.0 30 8907.5 31 10314.5 32 11590.5 33 12858.0 34 14004.0 35 14966.5 36 16534.0 37 18476.0 38 19738.0 39 20312.5 40 20678.0 41 21827.0 42 23079.5 43 24356.5 44 25993.5 45 27200.0 46 27514.0 47 27452.5 48 27917.0 49 27898.5 50 27213.5 51 26732.0 52 26314.0 53 26353.0 54 26091.0 55 24703.5 56 22178.5 57 19847.5 58 18589.5 59 16711.0 60 14393.5 61 12081.5 62 9944.0 63 7792.5 64 5877.5 65 4532.5 66 3331.0 67 2675.0 68 2010.5 69 1306.0 70 982.0 71 754.0 72 606.5 73 513.5 74 397.0 75 328.5 76 236.0 77 112.5 78 53.0 79 28.0 80 13.5 81 7.5 82 5.0 83 1.5 84 1.5 85 1.0 86 2.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019163468349879996 2 0.0019824277603324135 3 1.3216185068882756E-4 4 0.1441885791015109 5 0.03872342225182648 6 0.012026728412683308 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.9824277603324136E-4 30-31 6.608092534441378E-5 32-33 1.9824277603324136E-4 34-35 4.625664774108965E-4 36-37 0.0015859422082659309 38-39 0.0025110751630877236 40-41 0.0 42-43 0.0011233757308550343 44-45 7.929711041329654E-4 46-47 1.9824277603324136E-4 48-49 0.002114589611021241 50-51 0.0011233757308550343 52-53 1.3216185068882756E-4 54-55 0.0 56-57 0.0016520231336103447 58-59 0.0019824277603324135 60-61 0.0013216185068882756 62-63 1.9824277603324136E-4 64-65 0.0016520231336103447 66-67 9.25132954821793E-4 68-69 0.0013876994322326896 70-71 0.0017841849842991722 72-73 0.0 74-75 1.9824277603324136E-4 76-77 0.0041630982966980685 78-79 8.590520294773791E-4 80-81 0.0 82-83 0.0 84-85 3.304046267220689E-4 86-87 2.6432370137765513E-4 88-89 0.0 90-91 1.9824277603324136E-4 92-93 8.590520294773791E-4 94-95 0.0012555375815438618 96-97 5.947283280997241E-4 98-99 0.0 100-101 3.964855520664827E-4 102-103 0.0 104-105 6.608092534441378E-5 106-107 3.964855520664827E-4 108-109 7.929711041329654E-4 110-111 0.0 112-113 1.9824277603324136E-4 114-115 0.002180670536365655 116-117 4.625664774108965E-4 118-119 4.625664774108965E-4 120-121 1.9824277603324136E-4 122-123 5.947283280997241E-4 124-125 0.0011233757308550343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 756648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.26400309239961 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85888606954755 48.92475043120349 2 11.724280060404705 14.365526597535858 3 3.6024870013681007 6.621083243764341 4 1.5580588437519884 3.8181168728704953 5 0.8566653705338441 2.624137495476854 6 0.5177689095329816 1.9032357644866174 7 0.34328562344959296 1.472173604761453 8 0.25215778583272624 1.2358556296823024 9 0.18789849021315885 1.0360272316928565 >10 1.0145072291244812 11.218391283988307 >50 0.05809096202786146 2.4368342624880266 >100 0.02483391862184245 2.7580132637512733 >500 4.3189423647445693E-4 0.22371566298841267 >1k 6.478413547116854E-4 1.3621386553096966 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4533 0.5990896691724553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.46098053520263055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2275 0.3006682103170827 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 959 0.12674321481058565 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.6432370137765513E-4 14-15 0.0 0.0 0.0 0.0 2.6432370137765513E-4 16-17 0.0 0.0 0.0 0.0 2.6432370137765513E-4 18-19 0.0 0.0 0.0 0.0 2.6432370137765513E-4 20-21 0.0 0.0 0.0 0.0 3.964855520664827E-4 22-23 0.0 0.0 0.0 6.608092534441378E-5 3.964855520664827E-4 24-25 0.0 0.0 0.0 1.3216185068882756E-4 3.964855520664827E-4 26-27 0.0 0.0 0.0 3.964855520664827E-4 5.94728328099724E-4 28-29 0.0 0.0 0.0 9.25132954821793E-4 6.608092534441378E-4 30-31 0.0 0.0 0.0 0.003105803491187448 6.608092534441378E-4 32-33 0.0 0.0 0.0 0.0063437688330637235 6.608092534441378E-4 34-35 0.0 0.0 0.0 0.009978219727006481 6.608092534441378E-4 36-37 0.0 0.0 0.0 0.02028684408073503 6.608092534441378E-4 38-39 0.0 0.0 0.0 0.03766612744631585 6.608092534441378E-4 40-41 0.0 0.0 0.0 0.05742432412429558 6.608092534441378E-4 42-43 0.0 0.0 0.0 0.07414279823643227 6.608092534441378E-4 44-45 0.0 0.0 0.0 0.09575126082405558 6.608092534441378E-4 46-47 0.0 0.0 0.0 0.12337308761802053 6.608092534441378E-4 48-49 0.0 0.0 0.0 0.1538363942017953 7.929711041329654E-4 50-51 0.0 0.0 0.0 0.1903791459172561 7.929711041329654E-4 52-53 0.0 0.0 0.0 0.2267897357820281 7.929711041329654E-4 54-55 0.0 0.0 0.0 0.2648523487804104 7.929711041329654E-4 56-57 0.0 0.0 0.0 0.309655216163923 7.929711041329654E-4 58-59 0.0 0.0 0.0 0.3628503610661761 9.25132954821793E-4 60-61 0.0 0.0 0.0 0.4254289973673359 9.25132954821793E-4 62-63 0.0 0.0 0.0 0.48496791110265275 9.25132954821793E-4 64-65 0.0 0.0 0.0 0.5439120965098698 9.25132954821793E-4 66-67 0.0 0.0 0.0 0.6137596345989152 9.25132954821793E-4 68-69 0.0 0.0 0.0 0.6971537623835654 9.25132954821793E-4 70-71 0.0 0.0 0.0 0.7845788266142248 9.25132954821793E-4 72-73 0.0 0.0 0.0 0.8810569776170689 0.0010572948055106205 74-75 0.0 0.0 0.0 0.9828216026474662 0.0010572948055106205 76-77 0.0 0.0 0.0 1.089013649675939 0.0010572948055106205 78-79 0.0 0.0 0.0 1.2258011651388756 0.0010572948055106205 80-81 0.0 0.0 0.0 1.372632981254163 0.0010572948055106205 82-83 0.0 0.0 0.0 1.5264032945306139 0.0010572948055106205 84-85 0.0 0.0 0.0 1.674490648227445 0.0010572948055106205 86-87 0.0 0.0 0.0 1.833217030904727 0.0010572948055106205 88-89 0.0 0.0 0.0 2.0280896797454036 0.0010572948055106205 90-91 0.0 0.0 0.0 2.2532934733191654 0.0010572948055106205 92-93 0.0 0.0 0.0 2.4872199490383906 0.001189456656199448 94-95 0.0 0.0 0.0 2.714075765745763 0.001189456656199448 96-97 0.0 0.0 0.0 2.950909802180142 0.0013876994322326894 98-99 0.0 0.0 0.0 3.2071716306657785 0.0014537803575771031 100-101 0.0 0.0 0.0 3.518809274590034 0.0014537803575771031 102-103 0.0 0.0 0.0 3.8447864793140267 0.0014537803575771031 104-105 0.0 0.0 0.0 4.16792220424821 0.0014537803575771031 106-107 0.0 0.0 0.0 4.4897363106755055 0.0014537803575771031 108-109 0.0 0.0 0.0 4.836925492435055 0.0014537803575771031 110-111 0.0 0.0 0.0 5.236516847992726 0.0014537803575771031 112-113 0.0 0.0 0.0 5.662474492762817 0.0014537803575771031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1820 0.0 75.329285 1 GTATCAA 3695 0.0 61.140945 1 TATAACG 110 3.6379788E-12 54.185856 2 TAACGCA 135 0.0 48.56658 4 ATCAACG 4715 0.0 46.899788 3 TCAACGC 4735 0.0 46.701687 4 CAACGCA 4790 0.0 45.986206 5 TATCAAC 4880 0.0 45.680454 2 AACGCAG 4965 0.0 44.34482 6 ATAACGC 140 4.0017767E-11 42.574604 3 GTGGTAT 690 0.0 38.875034 1 ACGCAGA 5715 0.0 38.413544 7 CGCAGAG 5785 0.0 37.845894 8 GCAGAGT 6360 0.0 34.2372 9 TGGTATC 785 0.0 33.40886 2 GTGTAGC 390 0.0 32.096825 1 GAGTACT 4125 0.0 30.79273 12-13 GTACATG 4070 0.0 30.463255 1 TACATGG 4145 0.0 30.053867 2 GTATAGG 260 1.8189894E-12 29.804193 1 >>END_MODULE