FastQCFastQC Report
Fri 27 May 2016
SRR940072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490930
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11800.24036013280915813No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT250.002037398571.565021
GGTATCA6050.058.15891
GTATCAA9950.046.7510151
GTACATG27250.039.6124421
TACATGG27000.038.4212882
ATCAACG11600.037.4845473
TCAACGC11650.037.331294
CAACGCA11650.037.3198625
TAGGACG650.001925607136.662554
AACGCAG12450.034.9218036
ACATGGG28450.034.126583
TATCAAC14100.032.5580252
GTATAGG1551.9275103E-730.7806531
CATGGGA18900.030.2611564
ACGCAGA15700.028.072127
CGCAGAG16050.027.4599538
GTAGCCC4150.027.270493
TAGCCCT5250.027.2350394
ACCGTGG900.00947828726.4704068
CATGGGG10600.024.1678874
Produced by FastQC (version 0.11.5)