FastQCFastQC Report
Fri 27 May 2016
SRR940072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490930
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.6891002790621881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.4964047827592528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.33528201576599514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.1403458741572118No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.13464241337868943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.069.0211261
TATAACG1050.068.080012
ATAACGC1200.064.534183
GTATCAA25500.058.88721
TAACGCA1400.051.0600054
TAGACCG505.322808E-447.612315
ATCAACG32150.045.766073
TCAACGC32350.045.4831284
CAACGCA32550.045.1622125
TATCAAC33400.044.766682
AACGCAG34500.042.9458356
GTGGTAT6000.038.7323571
TGGTATC5700.038.668252
CGCAGAG40500.036.2859468
ACGCAGA40750.036.209347
GTATAAC3150.035.9421121
GCAGAGT45000.032.392929
GTATAGG2351.0913936E-1130.4280381
GTACATG26900.029.4618431
TACATGG26600.029.1131612
Produced by FastQC (version 0.11.5)