##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940072_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490930 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.143869798138226 34.0 33.0 34.0 31.0 34.0 2 33.18202187684599 34.0 33.0 34.0 31.0 34.0 3 33.24318334589453 34.0 33.0 34.0 31.0 34.0 4 36.46254659523761 37.0 37.0 37.0 35.0 37.0 5 36.48164504104455 37.0 37.0 37.0 35.0 37.0 6 36.56185606909335 37.0 37.0 37.0 35.0 37.0 7 36.55635834029292 37.0 37.0 37.0 35.0 37.0 8 36.55326828672112 37.0 37.0 37.0 35.0 37.0 9 38.42723606216772 39.0 39.0 39.0 37.0 39.0 10-11 38.40942802436193 39.0 39.0 39.0 37.0 39.0 12-13 38.39362230867945 39.0 39.0 39.0 37.0 39.0 14-15 39.9932872303587 41.0 40.0 41.0 38.0 41.0 16-17 40.00423583810319 41.0 40.0 41.0 38.0 41.0 18-19 40.0628073248732 41.0 40.0 41.0 38.0 41.0 20-21 40.06219827674006 41.0 40.0 41.0 38.0 41.0 22-23 40.01496751064306 41.0 40.0 41.0 38.0 41.0 24-25 39.99027050699692 41.0 40.0 41.0 38.0 41.0 26-27 39.899124111380445 41.0 40.0 41.0 38.0 41.0 28-29 39.81375450675249 41.0 40.0 41.0 37.0 41.0 30-31 39.64569796101277 41.0 40.0 41.0 36.5 41.0 32-33 39.44470087385167 41.0 40.0 41.0 35.0 41.0 34-35 39.31692705681054 41.0 40.0 41.0 35.0 41.0 36-37 39.21103212270589 41.0 39.5 41.0 35.0 41.0 38-39 39.092048764589656 41.0 39.0 41.0 35.0 41.0 40-41 38.96065121300389 41.0 39.0 41.0 35.0 41.0 42-43 38.785413399058925 41.0 39.0 41.0 35.0 41.0 44-45 38.540717617582956 41.0 39.0 41.0 35.0 41.0 46-47 38.197368260240765 41.0 39.0 41.0 34.5 41.0 48-49 37.8827948994765 41.0 38.0 41.0 34.0 41.0 50-51 37.88901981952621 41.0 39.0 41.0 34.0 41.0 52-53 37.79280651009309 41.0 38.5 41.0 34.0 41.0 54-55 37.64645265109078 41.0 38.0 41.0 34.0 41.0 56-57 37.484713706638416 41.0 37.5 41.0 34.0 41.0 58-59 37.2917157232192 40.0 37.0 41.0 33.0 41.0 60-61 37.07719023078647 40.0 36.5 41.0 33.0 41.0 62-63 36.8316908724258 39.5 36.0 41.0 33.0 41.0 64-65 36.54730205935673 39.0 35.0 41.0 32.5 41.0 66-67 36.23976228790255 39.0 35.0 41.0 32.0 41.0 68-69 35.8986454280651 38.0 35.0 41.0 32.0 41.0 70-71 35.53860326319435 37.0 35.0 40.0 31.5 41.0 72-73 35.17299207626341 37.0 35.0 39.0 31.0 41.0 74-75 34.80774855885768 36.0 35.0 39.0 31.0 41.0 76-77 34.43394679485874 36.0 35.0 38.5 31.0 40.5 78-79 34.09046503574848 35.0 35.0 37.0 31.0 39.0 80-81 33.76946713380727 35.0 35.0 37.0 30.0 39.0 82-83 33.472641720815595 35.0 35.0 36.5 30.0 39.0 84-85 33.214073289471 35.0 35.0 36.0 30.0 37.0 86-87 33.00178844234412 35.0 35.0 36.0 30.0 37.0 88-89 32.872940134031325 35.0 35.0 35.5 29.5 37.0 90-91 32.85426944778278 35.0 35.0 35.0 30.0 36.0 92-93 32.751750758763976 35.0 35.0 35.0 30.0 36.0 94-95 32.669688142912435 35.0 35.0 35.0 30.0 36.0 96-97 32.59544130527774 35.0 35.0 35.0 30.0 36.0 98-99 32.536299472429874 35.0 35.0 35.0 30.0 36.0 100-101 32.47809565518506 35.0 35.0 35.0 30.0 35.0 102-103 32.01606237141751 35.0 33.5 35.0 28.0 35.0 104-105 32.3832358992117 35.0 34.5 35.0 29.0 35.0 106-107 32.42466950481739 35.0 35.0 35.0 30.0 35.0 108-109 32.411891715723215 35.0 35.0 35.0 30.0 35.0 110-111 32.3761106471391 35.0 35.0 35.0 30.0 35.0 112-113 32.34043040759375 35.0 35.0 35.0 29.5 35.0 114-115 32.290228749516224 35.0 35.0 35.0 29.0 35.0 116-117 32.241818589208236 35.0 35.0 35.0 29.0 35.0 118-119 32.18488786588719 35.0 35.0 35.0 29.0 35.0 120-121 32.103946591163705 35.0 35.0 35.0 29.0 35.0 122-123 32.01053612531318 35.0 35.0 35.0 29.0 35.0 124-125 30.99492799380767 34.5 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01826632831707542 1101 2 0.048952646777919995 1101 3 0.07423994491186647 1101 4 0.005804628839896964 1101 5 -0.041594289426811315 1101 6 0.017703918348679792 1101 7 0.037030654586494904 1101 8 0.04792501534920035 1101 9 0.08910907053349604 1101 10-11 0.11295393090417605 1101 12-13 0.07011468589960401 1101 14-15 0.1623482943200969 1101 16-17 0.11954352917078381 1101 18-19 0.10425805955154743 1101 20-21 0.06005857815429749 1101 22-23 0.13848526316732546 1101 24-25 0.07126380564773882 1101 26-27 0.07053631990221021 1101 28-29 0.11079048859407692 1101 30-31 0.046194857598919725 1101 32-33 0.03262833202094839 1101 34-35 0.011731584404380158 1101 36-37 0.03865162322233573 1101 38-39 -0.1370717225575362 1101 40-41 -0.1676922066655564 1101 42-43 -0.2759136423998285 1101 44-45 -0.4822514599777534 1101 46-47 -0.5982261970492999 1101 48-49 -0.7507399782356003 1101 50-51 -0.7931057945468041 1101 52-53 -0.796125532120243 1101 54-55 -0.7622784705762982 1101 56-57 -0.8002386964301351 1101 58-59 -0.9300474200193563 1101 60-61 -0.7785613633949211 1101 62-63 -0.7647067804755423 1101 64-65 -0.7862636378229411 1101 66-67 -0.6578448653385252 1101 68-69 -0.6712894795742841 1101 70-71 -0.6385189977226702 1101 72-73 -0.5523331549199568 1101 74-75 -0.6925063798951072 1101 76-77 -0.52676135235901 1101 78-79 -0.5538588647291789 1101 80-81 -0.5507704542142093 1101 82-83 -0.5654447428676193 1101 84-85 -0.6403253386384335 1101 86-87 -0.5926852024464893 1101 88-89 -0.5879819048786814 1101 90-91 -0.6300367708294488 1101 92-93 -0.7324684215104469 1101 94-95 -0.7693098053525738 1101 96-97 -0.8024256241636891 1101 98-99 -0.8833013612402993 1101 100-101 -0.770325152078307 1101 102-103 -0.7315318629739522 1101 104-105 -0.6763471835797752 1101 106-107 -0.7329730169349844 1101 108-109 -0.706391882660256 1101 110-111 -0.7831658477150967 1101 112-113 -0.8080325668665687 1101 114-115 -0.6605846832879649 1101 116-117 -0.6196350473180701 1101 118-119 -0.7269362916357167 1101 120-121 -0.9166205070012943 1101 122-123 -0.7541313864026868 1101 124-125 -0.6320029276183909 1102 1 0.093237315405311 1102 2 0.08591333800208645 1102 3 0.055079249900465754 1102 4 0.0568240708722314 1102 5 0.07024000288808452 1102 6 0.12579426009237693 1102 7 0.06628162100725632 1102 8 0.060717748189645704 1102 9 0.08243542453701025 1102 10-11 0.07427140114058517 1102 12-13 0.06855650993933438 1102 14-15 0.15439645710620198 1102 16-17 0.2537089717495604 1102 18-19 0.21122440457390468 1102 20-21 0.18604359694167982 1102 22-23 0.1411985452648068 1102 24-25 0.17608901130802934 1102 26-27 0.14936487455875636 1102 28-29 0.0592800588242568 1102 30-31 -0.08748365499228328 1102 32-33 -0.18987026805532992 1102 34-35 -0.17822791925165404 1102 36-37 -0.2782951634455202 1102 38-39 -0.2354775678815173 1102 40-41 -0.24664384640228576 1102 42-43 -0.17007535184870903 1102 44-45 -0.18197255730153472 1102 46-47 -0.17492172784356086 1102 48-49 -0.5156190708145161 1102 50-51 -0.5250062011333512 1102 52-53 -0.5252328541946696 1102 54-55 -0.4515954971653784 1102 56-57 -0.3534844905723631 1102 58-59 -0.44774191473481295 1102 60-61 -0.4996248725911414 1102 62-63 -0.4907599694611804 1102 64-65 -0.46288667015239326 1102 66-67 -0.47840169683099276 1102 68-69 -0.3675276749035419 1102 70-71 -0.5364557671586141 1102 72-73 -0.6135023820268088 1102 74-75 -0.5500685378606107 1102 76-77 -0.437796538460951 1102 78-79 -0.5777982365353864 1102 80-81 -0.4685942558930378 1102 82-83 -0.4880255800084754 1102 84-85 -0.4213975584710852 1102 86-87 -0.5571027936038462 1102 88-89 -0.40745396456547667 1102 90-91 -0.3811803800086224 1102 92-93 -0.3351822446412456 1102 94-95 -0.41843752438315107 1102 96-97 -0.3058950003252221 1102 98-99 -0.4109008234531828 1102 100-101 -0.397465277002901 1102 102-103 -0.4426755262564548 1102 104-105 -0.44140752232073766 1102 106-107 -0.3969886203359181 1102 108-109 -0.39754706995245925 1102 110-111 -0.3910411287142779 1102 112-113 -0.42228663070309125 1102 114-115 -0.39582867578924663 1102 116-117 -0.3064011018055339 1102 118-119 -0.3433828929996636 1102 120-121 -0.3967064230924393 1102 122-123 -0.37976473353236884 1102 124-125 -0.28255356427935396 1103 1 0.17689822665243327 1103 2 0.18900501005927595 1103 3 0.17415174828330748 1103 4 0.079945266407222 1103 5 0.10959081810096905 1103 6 0.0978796459708633 1103 7 0.11566710142722059 1103 8 0.12681384876850643 1103 9 0.17987948749831872 1103 10-11 0.1464131801789179 1103 12-13 0.22864673899508148 1103 14-15 0.21828658996185624 1103 16-17 0.2852103566992241 1103 18-19 0.31608250943200744 1103 20-21 0.2120590884744118 1103 22-23 0.2172049143746193 1103 24-25 0.20181839048568406 1103 26-27 0.13022851921549972 1103 28-29 0.2221609199809862 1103 30-31 0.23081841330979103 1103 32-33 0.33567666266401375 1103 34-35 0.3862344383141547 1103 36-37 0.5249880794928998 1103 38-39 0.3596278375687092 1103 40-41 0.32604527801098016 1103 42-43 0.33231721088867516 1103 44-45 0.3205195227767561 1103 46-47 0.6851900633027128 1103 48-49 0.6962699341779413 1103 50-51 0.5864863931178661 1103 52-53 0.5139272194481563 1103 54-55 0.546915889277038 1103 56-57 0.5823765832197481 1103 58-59 0.5321472892923182 1103 60-61 0.6769603557182222 1103 62-63 0.6159018872006783 1103 64-65 0.6862317720694975 1103 66-67 0.5070107196158986 1103 68-69 0.5556615241477232 1103 70-71 0.6434384127355628 1103 72-73 0.6633880327946358 1103 74-75 0.6040589438530759 1103 76-77 0.5807012472782134 1103 78-79 0.4983532620298945 1103 80-81 0.4643061770073942 1103 82-83 0.46071054976214754 1103 84-85 0.4507931499265112 1103 86-87 0.4736397716214782 1103 88-89 0.4501159878320635 1103 90-91 0.5036365910152512 1103 92-93 0.3463912903805735 1103 94-95 0.38274756128469534 1103 96-97 0.3111830046838264 1103 98-99 0.3734368057120321 1103 100-101 0.47089401377018447 1103 102-103 0.3843135765947139 1103 104-105 0.4334867405045628 1103 106-107 0.511986185190608 1103 108-109 0.5632845632929602 1103 110-111 0.592716811940214 1103 112-113 0.6162514595591375 1103 114-115 0.685734078187302 1103 116-117 0.7803033814506719 1103 118-119 0.6702924482618826 1103 120-121 0.6286995247273026 1103 122-123 0.6613898334842645 1103 124-125 0.6221295559210098 1104 1 0.07606446624420471 1104 2 0.07189556316259882 1104 3 0.08350716827703053 1104 4 0.038769735869990996 1104 5 -0.010147205892380384 1104 6 0.013864370466230014 1104 7 0.039694610561113564 1104 8 0.03251020127336801 1104 9 0.11181201517552353 1104 10-11 0.12422181756202377 1104 12-13 0.1038334120540938 1104 14-15 0.08348522111793955 1104 16-17 0.08666874858315055 1104 18-19 0.08344792573359427 1104 20-21 0.19663755177627706 1104 22-23 0.1938122314074704 1104 24-25 0.21230652331423983 1104 26-27 0.11229275127973182 1104 28-29 0.19877788064049895 1104 30-31 0.138960114004675 1104 32-33 0.14637089357101019 1104 34-35 0.2189808880818802 1104 36-37 0.21207030380917047 1104 38-39 0.27641711606011654 1104 40-41 0.10781827254992749 1104 42-43 0.08646121503268489 1104 44-45 0.36102137710839344 1104 46-47 0.17114343223480688 1104 48-49 0.021563238194644896 1104 50-51 -0.04671413369968036 1104 52-53 -0.006522779725237626 1104 54-55 -0.011195227344714453 1104 56-57 0.08134333750565048 1104 58-59 -0.0561552670953418 1104 60-61 -0.09076325243091077 1104 62-63 -0.06066878405084708 1104 64-65 0.003217780544865434 1104 66-67 -0.029265848575555253 1104 68-69 0.032445181782449595 1104 70-71 0.04284436533300351 1104 72-73 -0.14481125412805085 1104 74-75 -0.13278577171504224 1104 76-77 -0.13173820452293938 1104 78-79 -0.09012425729868312 1104 80-81 -0.10626863824826671 1104 82-83 -0.06741439857557197 1104 84-85 -0.07215179323119969 1104 86-87 0.001159926801207689 1104 88-89 -0.02434355577641867 1104 90-91 0.010891353589158825 1104 92-93 -0.12216635370899098 1104 94-95 -0.11077880990124811 1104 96-97 -0.15958716289485153 1104 98-99 -0.1739988161916699 1104 100-101 -0.11951774153519068 1104 102-103 0.016139197782045756 1104 104-105 -0.007514929220512556 1104 106-107 0.09718900443598244 1104 108-109 -0.007254432777948239 1104 110-111 -0.041581710656174664 1104 112-113 0.005942147122169672 1104 114-115 -0.06242949976351042 1104 116-117 -0.10323742762205512 1104 118-119 -0.1644681418178564 1104 120-121 -0.15761010732700953 1104 122-123 -0.1445101373093216 1104 124-125 -0.1317912671487491 1105 1 0.062242901470789036 1105 2 0.06983081193771312 1105 3 0.08909061059059553 1105 4 0.04680686484776686 1105 5 0.07654102189327716 1105 6 0.06438682300435516 1105 7 0.046820743107183205 1105 8 -6.901495645905698E-5 1105 9 -0.08416446075615625 1105 10-11 0.05461803575219193 1105 12-13 0.0509930087097743 1105 14-15 0.1536932885264477 1105 16-17 0.14756194273501677 1105 18-19 0.1543777424640851 1105 20-21 0.11308691055486264 1105 22-23 0.16723149598014686 1105 24-25 0.11813063574530247 1105 26-27 0.11051382844817681 1105 28-29 0.12722028556666487 1105 30-31 0.13773849128249793 1105 32-33 0.05572805376803558 1105 34-35 0.04785059993031382 1105 36-37 0.17309192496516346 1105 38-39 0.10488745124937537 1105 40-41 0.04791307882614859 1105 42-43 -0.044565652836290326 1105 44-45 -0.03855366524380344 1105 46-47 0.169595521392381 1105 48-49 0.14222066960235935 1105 50-51 0.0597275768853649 1105 52-53 -0.0636910686964427 1105 54-55 -0.11226362516563171 1105 56-57 -0.14197216744479846 1105 58-59 -0.13700032722371702 1105 60-61 -0.015844889718593436 1105 62-63 0.0898962164581576 1105 64-65 0.06679175789264491 1105 66-67 -0.008229400887373117 1105 68-69 0.05583873485124968 1105 70-71 0.01230249212596135 1105 72-73 0.08878232660998009 1105 74-75 0.18061396777652305 1105 76-77 0.08216323558230698 1105 78-79 0.11234404707332146 1105 80-81 0.09349277987820415 1105 82-83 0.07806833817256376 1105 84-85 -0.06573174817732763 1105 86-87 -0.07801714845649599 1105 88-89 -0.11842025175680959 1105 90-91 0.0015986679246964286 1105 92-93 0.07450576849505097 1105 94-95 0.06372399226112435 1105 96-97 0.005981870535322287 1105 98-99 0.09025410147670243 1105 100-101 0.02108894554406504 1105 102-103 0.1036118222087552 1105 104-105 -0.03793803355179648 1105 106-107 -0.14581994629973138 1105 108-109 -0.182683893201812 1105 110-111 -0.10128460574541265 1105 112-113 -0.11554014065351481 1105 114-115 -0.1320933185874651 1105 116-117 -0.13304058452487055 1105 118-119 -0.02942401461247357 1105 120-121 -0.06370699662658552 1105 122-123 -0.04457330668940074 1105 124-125 -0.098276256590065 1106 1 0.1310763236876724 1106 2 0.1362931716331559 1106 3 0.10715525271538695 1106 4 -0.06788045284707067 1106 5 -0.012385089589230347 1106 6 0.03717844241251811 1106 7 0.09858101291256105 1106 8 0.10736240074562886 1106 9 0.021662043566585965 1106 10-11 0.12337008570725061 1106 12-13 0.17178825356516825 1106 14-15 0.17704212014595555 1106 16-17 0.20604481324796353 1106 18-19 0.1704080780432946 1106 20-21 0.1477715241868509 1106 22-23 0.171901011927865 1106 24-25 0.224447877400884 1106 26-27 0.2739881986894659 1106 28-29 0.26456164095313284 1106 30-31 0.22334837012545705 1106 32-33 0.1424796480384316 1106 34-35 0.10705330199016316 1106 36-37 0.44397517705142775 1106 38-39 0.47134680643053173 1106 40-41 0.5540923632009154 1106 42-43 0.5582037582038026 1106 44-45 0.4511045462189216 1106 46-47 0.4612499822197833 1106 48-49 0.6783487019709966 1106 50-51 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-0.42425879724599014 2216 92-93 -0.33722477738183443 2216 94-95 -0.43044241044186293 2216 96-97 -0.32618516012337295 2216 98-99 -0.3465710545397229 2216 100-101 -0.3825768153104008 2216 102-103 -0.3536100851094339 2216 104-105 -0.29662799570773757 2216 106-107 -0.3508113605337684 2216 108-109 -0.26985373021118875 2216 110-111 -0.2997429189341503 2216 112-113 -0.3270535416726972 2216 114-115 -0.491422211773866 2216 116-117 -0.5321460255109329 2216 118-119 -0.529003062009231 2216 120-121 -0.5283046048378033 2216 122-123 -0.48553088355840046 2216 124-125 -0.6445324180580343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 4.0 5 23.0 6 58.0 7 107.0 8 255.0 9 438.0 10 865.0 11 1294.0 12 1647.0 13 2575.0 14 4818.0 15 5012.0 16 2047.0 17 1464.0 18 1315.0 19 1133.0 20 1017.0 21 923.0 22 950.0 23 992.0 24 1147.0 25 1460.0 26 1814.0 27 2161.0 28 2621.0 29 3410.0 30 4013.0 31 5003.0 32 6520.0 33 8674.0 34 12979.0 35 23687.0 36 53463.0 37 185755.0 38 149665.0 39 1614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.685870754411376 18.8463285883386 14.151248999150424 22.3165516580996 2 15.224489879062075 19.953637406220654 41.96873987855282 22.853132836164452 3 19.3818250704277 25.830619091559065 29.168780006884905 25.618775831128332 4 12.126274648993437 16.754890021683565 38.34156411339098 32.77727121593201 5 12.941377848782237 38.03002021453491 34.00524925825699 15.023352678425875 6 30.30769136740627 37.35772100992362 17.798549044614536 14.536038578055575 7 27.628378791273704 33.375430305746235 20.906239178701647 18.08995172427841 8 25.787790520033404 35.625649277901125 19.605646426170736 18.98091377589473 9 26.82378343144644 16.47852035931803 20.451388181614487 36.24630802762104 10-11 24.865052044079604 27.211516916872057 27.0131179597906 20.910313079257737 12-13 25.99902226386654 24.586906483612736 27.9588739738863 21.455197278634426 14-15 22.848267573788526 25.32682867211211 27.106104739983294 24.718799014116065 16-17 21.28388976025095 28.843317784612875 27.533966145886378 22.33882630924979 18-19 21.055751329110056 26.674678671093638 31.25496506630273 21.014604933493576 20-21 22.236978795347607 25.655185056932762 31.499297252154072 20.60853889556556 22-23 21.620903183753285 25.80714154767482 31.344590878536653 21.227364390035238 24-25 21.295805919377507 25.876397857128307 31.61805145336402 21.20974477013016 26-27 21.163302303790765 26.15423787505347 31.469048540525122 21.21341128063064 28-29 21.039927402870276 26.176316917840374 31.517318713417534 21.26643696587181 30-31 21.428920620047663 25.93852484060864 31.450410445481026 21.182144093862668 32-33 21.11413145879398 25.96442055325663 31.475700686147402 21.44574730180199 34-35 21.50944587653803 26.01336039769539 31.271967302774232 21.20522642299235 36-37 21.58424029837673 26.010392668504025 30.999667972370414 21.405699060748834 38-39 21.352037334669625 26.23112947153354 31.018147892387827 21.398685301409014 40-41 21.652679608090768 26.04179821970546 30.931293667121583 21.37422850508219 42-43 21.496097155567867 25.897593110135176 30.87901589655425 21.727293837742707 44-45 21.670418088302693 26.167948260935987 30.52900137495544 21.63263227580588 46-47 21.91290000611085 26.503473000224066 29.7857128307498 21.797914162915283 48-49 21.890331405338294 26.598998420327362 29.556377813696404 21.95429236063794 50-51 22.048103805629253 26.917964831157988 29.213666247383724 21.820265115829034 52-53 22.075244943270935 26.968101358645836 29.00230175381419 21.95435194426904 54-55 21.945083820503942 26.96779581610413 29.185627278838123 21.901493084553806 56-57 22.025156592147475 27.0584101441157 28.979477516932324 21.9369557468045 58-59 22.015456662069866 26.80838668543411 29.182088360449033 21.99406829204699 60-61 22.09912114795656 26.855555182110486 29.330375648393996 21.714948021538955 62-63 21.895732376779534 26.787987672351804 29.42350115800859 21.892778792860067 64-65 21.91702831279224 26.64736982012582 29.38769856587493 22.047903301207004 66-67 22.06254697745286 26.935674754087675 29.002988224318482 21.998790044140982 68-69 22.125047486838632 26.808548343893317 29.2138012794202 21.85260288984785 70-71 21.956714365738147 26.6167948260936 29.240006110913075 22.18648469725518 72-73 22.02595074654228 26.798932638054307 28.980200843297414 22.194915772106004 74-75 22.045912859267105 27.01067361945695 28.796569775731772 22.14684374554417 76-77 22.00684034376903 26.908950909635355 28.87560066896036 22.208608077635255 78-79 21.99422718884059 26.89001511441736 28.888499598714258 22.227258098027793 80-81 21.845171409365896 26.964333000631452 28.84240115698776 22.34809443301489 82-83 22.036237345446395 27.05334772778196 28.675880471757687 22.23453445501395 84-85 22.032852201757493 26.900693790917572 28.651772364877804 22.414681642447132 86-87 21.90567853705486 26.851418997713512 28.836437152125317 22.406465313106313 88-89 22.016886317804982 26.764813720897074 28.79585684313446 22.422443118163486 90-91 22.002083806501354 26.855336367719534 28.874367652315968 22.26821217346314 92-93 21.927776861137083 26.843211664558392 28.81052629434759 22.41848517995694 94-95 21.773736871695007 26.884405732862927 28.777163063936577 22.56469433150549 96-97 21.95742730559658 26.855935224321435 28.732291083159346 22.454346386922648 98-99 21.783655510968977 26.726824598211557 28.608762960096147 22.88075693072332 100-101 21.752432632824707 26.710888947281582 28.77422193433266 22.762456485561046 102-103 21.90403927240136 26.47291874605341 28.77986678345182 22.843175198093416 104-105 21.762980465646834 26.567229543926835 28.791884790092276 22.87790520033406 106-107 21.75508067358009 26.530618480472732 28.706595047349005 23.00770579859817 108-109 21.783823917332157 26.51597941451759 28.544380699022366 23.15581596912789 110-111 21.59584869533335 26.703501517527954 28.51781313018149 23.182836656957203 112-113 21.60254294944656 26.68548824059023 28.658290658877306 23.053678151085908 114-115 21.44761714644222 26.657792244828123 28.650870603189087 23.24372000554057 116-117 21.42311523395726 26.663040843472395 28.611615263493462 23.30222865907688 118-119 21.35959114084171 26.66781415566877 28.49048840255272 23.4821063009368 120-121 21.326178198702664 26.704750885819657 28.60237569752887 23.36669521794881 122-123 21.313650941733997 26.622050620302918 28.48871375625217 23.575584681710918 124-125 21.167055046768567 26.681611152755597 28.46822752012515 23.68310628035068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 129.0 2 346.5 3 519.5 4 262.5 5 130.5 6 314.5 7 850.5 8 1556.0 9 1677.0 10 1093.0 11 571.5 12 463.0 13 510.5 14 550.0 15 574.5 16 664.5 17 735.5 18 766.5 19 835.0 20 921.5 21 1089.5 22 1308.5 23 1580.5 24 1891.5 25 2330.5 26 2906.5 27 3516.0 28 4166.5 29 5086.0 30 6145.5 31 7153.0 32 8232.5 33 9167.5 34 10359.0 35 11453.0 36 12663.0 37 13930.0 38 14562.0 39 15141.0 40 15449.0 41 15820.0 42 16319.5 43 16729.0 44 17468.5 45 18296.0 46 18308.0 47 18044.5 48 18096.5 49 18015.5 50 17671.5 51 16915.0 52 16164.0 53 15727.5 54 15170.0 55 14084.5 56 12555.0 57 11175.5 58 10299.0 59 9038.5 60 7492.0 61 6279.5 62 5006.0 63 3686.5 64 2742.0 65 2104.0 66 1514.0 67 1173.5 68 928.5 69 618.5 70 442.5 71 320.5 72 243.0 73 230.5 74 195.0 75 138.5 76 92.5 77 57.5 78 36.5 79 19.5 80 10.0 81 6.0 82 2.5 83 1.5 84 1.5 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019758417697024015 2 0.0034628154726743117 3 2.036950278043713E-4 4 0.1419754343796468 5 0.03951683539404803 6 0.016499297252154075 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.0554254170655695E-4 30-31 0.0 32-33 1.0184751390218565E-4 34-35 3.0554254170655695E-4 36-37 0.001425865194630599 38-39 0.0038702055282830547 40-41 0.0 42-43 0.0012221701668262278 44-45 0.0010184751390218565 46-47 0.0 48-49 0.001120322652924042 50-51 0.002546187847554641 52-53 0.0 54-55 0.0 56-57 0.0010184751390218565 58-59 0.0018332552502393414 60-61 0.001120322652924042 62-63 2.036950278043713E-4 64-65 7.129325973152995E-4 66-67 6.110850834131139E-4 68-69 9.166276251196707E-4 70-71 0.0010184751390218565 72-73 0.0 74-75 0.0 76-77 0.0038702055282830547 78-79 0.0016295602224349703 80-81 0.0 82-83 0.0 84-85 8.147801112174852E-4 86-87 5.092375695109283E-4 88-89 0.0 90-91 3.0554254170655695E-4 92-93 0.001425865194630599 94-95 0.0012221701668262278 96-97 0.0010184751390218565 98-99 0.0 100-101 2.036950278043713E-4 102-103 0.0 104-105 0.0 106-107 2.036950278043713E-4 108-109 3.0554254170655695E-4 110-111 0.0 112-113 8.147801112174852E-4 114-115 0.0012221701668262278 116-117 0.001425865194630599 118-119 6.110850834131139E-4 120-121 1.0184751390218565E-4 122-123 1.0184751390218565E-4 124-125 4.073900556087426E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 490930.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.0481769153304 #Duplication Level Percentage of deduplicated Percentage of total 1 84.42863289465934 60.829290795139016 2 10.13461672968916 14.60361318219424 3 2.5738590425424483 5.563255549566638 4 1.0114412400821808 2.914899896196086 5 0.5224328512745763 1.8820167247505588 6 0.3177856271259154 1.3737525050590307 7 0.20715612819872548 1.044765496148965 8 0.14614270673055318 0.8423452471526516 9 0.10387322638399799 0.6735488932152409 >10 0.5229084040390429 6.489093472210677 >50 0.02123942096639844 1.0005869985234919 >100 0.008495767120871106 0.9840600535057809 >500 5.663844747246898E-4 0.2754470396501613 >1k 8.495767120870347E-4 1.5233241466874847 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3383 0.6891002790621881 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.4964047827592528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1646 0.33528201576599514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.1403458741572118 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.13464241337868943 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.036950278043713E-4 0.0 20-21 0.0 0.0 0.0 2.036950278043713E-4 0.0 22-23 0.0 0.0 0.0 2.036950278043713E-4 0.0 24-25 0.0 0.0 0.0 2.036950278043713E-4 0.0 26-27 0.0 0.0 0.0 2.036950278043713E-4 0.0 28-29 0.0 0.0 0.0 6.110850834131139E-4 0.0 30-31 0.0 0.0 0.0 0.0016295602224349703 0.0 32-33 0.0 0.0 0.0 0.004990528181207096 0.0 34-35 0.0 0.0 0.0 0.010897683987533865 0.0 36-37 0.0 0.0 0.0 0.021387977919458984 0.0 38-39 0.0 0.0 0.0 0.03880390279673274 0.0 40-41 0.0 0.0 0.0 0.06039557574399609 0.0 42-43 0.0 0.0 0.0 0.07995029841321573 0.0 44-45 0.0 0.0 0.0 0.10714358462509929 0.0 46-47 0.0 0.0 0.0 0.13790153382355935 0.0 48-49 0.0 0.0 0.0 0.17263153606420467 0.0 50-51 0.0 0.0 0.0 0.21825922229238387 0.0 52-53 0.0 0.0 0.0 0.26011855050618216 0.0 54-55 0.0 0.0 0.0 0.3012649461226652 0.0 56-57 0.0 0.0 0.0 0.3506609903652252 0.0 58-59 0.0 0.0 0.0 0.4039272401360683 0.0 60-61 0.0 0.0 0.0 0.46452651090786873 0.0 62-63 0.0 0.0 0.0 0.5297089198052676 0.0 64-65 0.0 0.0 0.0 0.5992707718004604 0.0 66-67 0.0 0.0 0.0 0.6723972867822297 0.0 68-69 0.0 0.0 0.0 0.7628378791273704 0.0 70-71 0.0 0.0 0.0 0.8648890880573605 0.0 72-73 0.0 0.0 0.0 0.9653107367649156 0.0 74-75 0.0 0.0 0.0 1.0679730307783188 0.0 76-77 0.0 0.0 0.0 1.1871346220438759 0.0 78-79 0.0 0.0 0.0 1.3262583260342615 0.0 80-81 0.0 0.0 0.0 1.4900291283889762 0.0 82-83 0.0 0.0 0.0 1.6670401075509746 0.0 84-85 0.0 0.0 0.0 1.8374309983093313 0.0 86-87 0.0 0.0 0.0 2.011793942109873 0.0 88-89 0.0 0.0 0.0 2.209378119080113 0.0 90-91 0.0 0.0 0.0 2.439757195526857 0.0 92-93 0.0 0.0 0.0 2.682256126127961 0.0 94-95 0.0 0.0 0.0 2.9255698368402827 0.0 96-97 0.0 0.0 0.0 3.1674576823579734 0.0 98-99 0.0 0.0 0.0 3.4408164096714398 0.0 100-101 0.0 0.0 0.0 3.747479274030921 0.0 102-103 0.0 0.0 0.0 4.086529647811297 0.0 104-105 0.0 0.0 0.0 4.426292954188988 0.0 106-107 0.0 0.0 0.0 4.775833621901289 0.0 108-109 0.0 0.0 0.0 5.130059275253091 0.0 110-111 0.0 0.0 0.0 5.538060415945247 0.0 112-113 0.0 0.0 0.0 5.984152526836819 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1295 0.0 69.021126 1 TATAACG 105 0.0 68.08001 2 ATAACGC 120 0.0 64.53418 3 GTATCAA 2550 0.0 58.8872 1 TAACGCA 140 0.0 51.060005 4 TAGACCG 50 5.322808E-4 47.61231 5 ATCAACG 3215 0.0 45.76607 3 TCAACGC 3235 0.0 45.483128 4 CAACGCA 3255 0.0 45.162212 5 TATCAAC 3340 0.0 44.76668 2 AACGCAG 3450 0.0 42.945835 6 GTGGTAT 600 0.0 38.732357 1 TGGTATC 570 0.0 38.66825 2 CGCAGAG 4050 0.0 36.285946 8 ACGCAGA 4075 0.0 36.20934 7 GTATAAC 315 0.0 35.942112 1 GCAGAGT 4500 0.0 32.39292 9 GTATAGG 235 1.0913936E-11 30.428038 1 GTACATG 2690 0.0 29.461843 1 TACATGG 2660 0.0 29.113161 2 >>END_MODULE