FastQCFastQC Report
Fri 27 May 2016
SRR940071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947725
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.050.97791
TCAACGC14250.045.635024
ATCAACG14450.044.991483
CAACGCA14700.044.1865585
AACGCAG16450.039.8481186
TATCAAC17550.038.772282
GGTATCA7800.038.2641451
GTACATG35700.033.2737271
TACATGG35600.032.191772
ACGCAGA20150.031.939627
ACATGGG33900.031.6697373
CATGGGG26500.029.0422634
CGCAGAG22300.028.5930148
ATGGGGA15900.026.6098375
GCAGAGT25850.023.041669
TACGACC1602.5636412E-422.3726734
GTAGCAC4450.021.4452763
CAGAGTA25000.019.8072724
GTGTAGC7650.019.5072141
TAAGGTA4304.7293724E-1119.4243374
Produced by FastQC (version 0.11.5)