FastQCFastQC Report
Fri 27 May 2016
SRR940071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947725
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.27413015378933764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19640.20723311087076948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.12377008098340764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.061.015891
GTATCAA27450.056.470771
TCAACGC31300.048.371584
ATCAACG31500.048.0644573
CAACGCA32150.047.02315
AACGCAG33400.045.244156
TATCAAC34350.044.599522
ACGCAGA40050.037.878257
CGCAGAG41450.036.311848
TGGTATC7600.032.9427262
GTGGTAT7600.032.1634751
GCAGAGT46700.031.8475029
ACATGGG31850.027.1368483
GTACATG34600.027.0530511
CAGAGTA44550.026.1734310-11
TACATGG34650.025.6334362
GAGTACT30600.024.78797312-13
AGAGTAC44600.023.47632210-11
GTACTTT31750.023.32802214-15
CACCGTA1552.1080126E-423.0288267
Produced by FastQC (version 0.11.5)