FastQCFastQC Report
Fri 27 May 2016
SRR940070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753596
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12190.16175775879914436No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA250.002046852171.485326
GTATCAA14700.051.122111
GGTATCA9300.050.664031
TCAACGC16750.043.412184
ATCAACG17050.042.995053
CAACGCA17400.042.1106035
TATCAAC18350.040.3060382
AACGCAG18300.039.388546
ACGCAGA21650.032.7434657
GTGGTAT4200.032.661351
GTACATG36650.032.5470471
TACATGG37700.031.6426682
CGCAGAG22250.031.5927628
ACATGGG37500.029.878883
CGTATAG800.00529872729.8212321
CCGTACG400.005373222329.73810468-69
CGCTCCG2100.026.90590548-49
GTATAGG2256.377377E-926.5077631
CGCGGAA2150.026.28192982-83
ATGGGTA3400.026.2813665
Produced by FastQC (version 0.11.5)